CA2114124A1 - Methode a une etape d'amplification et de sequencage d'acides nucleiques - Google Patents

Methode a une etape d'amplification et de sequencage d'acides nucleiques

Info

Publication number
CA2114124A1
CA2114124A1 CA002114124A CA2114124A CA2114124A1 CA 2114124 A1 CA2114124 A1 CA 2114124A1 CA 002114124 A CA002114124 A CA 002114124A CA 2114124 A CA2114124 A CA 2114124A CA 2114124 A1 CA2114124 A1 CA 2114124A1
Authority
CA
Canada
Prior art keywords
nucleic acid
primer
strands
steps
primers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002114124A
Other languages
English (en)
Inventor
Keith Gregg
Christopher Lyle Cooper
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UNIVERSITY PARTNERSHIPS Pty Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2114124A1 publication Critical patent/CA2114124A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002114124A 1991-07-24 1992-07-23 Methode a une etape d'amplification et de sequencage d'acides nucleiques Abandoned CA2114124A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPK7400 1991-07-24
AUPK740091 1991-07-24

Publications (1)

Publication Number Publication Date
CA2114124A1 true CA2114124A1 (fr) 1993-02-04

Family

ID=3775575

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002114124A Abandoned CA2114124A1 (fr) 1991-07-24 1992-07-23 Methode a une etape d'amplification et de sequencage d'acides nucleiques

Country Status (4)

Country Link
EP (1) EP0595982A4 (fr)
JP (1) JPH07500004A (fr)
CA (1) CA2114124A1 (fr)
WO (1) WO1993002212A1 (fr)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5480783A (en) * 1994-03-31 1996-01-02 The Perkin-Elmer Corporation Method for reducing background signals in DNA replication/detection assays
US6403303B1 (en) 1996-05-14 2002-06-11 Visible Genetics Inc. Method and reagents for testing for mutations in the BRCA1 gene
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
DE655506T1 (de) * 1994-10-17 1995-09-28 Harvard College DNS Polymerase mit Veränderten Nukleotiden Bindungstelle.
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
AU7236296A (en) * 1995-09-08 1997-03-27 Life Technologies, Inc. Cloned dna polymerases from thermotoga and mutants thereof
US5795722A (en) * 1997-03-18 1998-08-18 Visible Genetics Inc. Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
US5830657A (en) * 1996-05-01 1998-11-03 Visible Genetics Inc. Method for single-tube sequencing of nucleic acid polymers
US5888736A (en) * 1995-12-22 1999-03-30 Visible Genetics, Inc. Method, compositions and kit for detection and identification of microorganisms
US5789168A (en) * 1996-05-01 1998-08-04 Visible Genetics Inc. Method for amplification and sequencing of nucleic acid polymers
US6083699A (en) * 1996-05-01 2000-07-04 Visible Genetics Inc. Method for bi-directional sequencing of nucleic acid polymers
US6214555B1 (en) 1996-05-01 2001-04-10 Visible Genetics Inc. Method compositions and kit for detection
US6413718B1 (en) 1996-05-01 2002-07-02 Visible Genetics Inc. Method for sequencing of nucleic acid polymers
WO1997041259A1 (fr) * 1996-05-01 1997-11-06 Visible Genetics Inc. Procede de sequencage de polymeres d'acide nucleique
US5928906A (en) * 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
AU4236597A (en) * 1996-08-27 1998-03-19 Visible Genetics Inc. Apparatus and method for performing sequencing of nucleic acid polymers
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
DE19653494A1 (de) * 1996-12-20 1998-06-25 Svante Dr Paeaebo Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen unter Zugabe einer zweiten thermostabilen DNA Polymerase und dessen Anwendung
DE19653439A1 (de) * 1996-12-20 1998-07-02 Svante Dr Paeaebo Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
US6197557B1 (en) 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
US7270958B2 (en) 1998-09-10 2007-09-18 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20020187477A1 (en) 2001-06-06 2002-12-12 Hong Xue Method for detecting single nucleotide polymorphisms (SNPs) and point mutations
US7222059B2 (en) 2001-11-15 2007-05-22 Siemens Medical Solutions Diagnostics Electrophoretic trace simulator
ATE469984T1 (de) 2003-12-01 2010-06-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
DE102012201717A1 (de) * 2012-02-06 2013-08-08 AusBio Laboratories Co, Ltd. Probenträger-Zentrifuge

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8801070D0 (sv) * 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
DK175170B1 (da) * 1988-11-29 2004-06-21 Sangtec Molecular Diagnostics Fremgangsmåde og reagenskombination til bestemmmelse af nukleotidsekvenser
DE3923895A1 (de) * 1989-07-19 1991-01-24 Basf Ag Verfahren zur sequenzierung von desoxyribonukleinsaeuren
US5108892A (en) * 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
AU5675490A (en) * 1990-04-13 1991-11-11 Consejo Superior De Investigaciones Cientificas (in vitro) dna synthesis reactions using phi 29 dna polymerase and a dna fragment encoding said polymerase

Also Published As

Publication number Publication date
WO1993002212A1 (fr) 1993-02-04
EP0595982A1 (fr) 1994-05-11
EP0595982A4 (en) 1995-10-11
JPH07500004A (ja) 1995-01-05

Similar Documents

Publication Publication Date Title
CA2114124A1 (fr) Methode a une etape d'amplification et de sequencage d'acides nucleiques
US6087099A (en) Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US5925520A (en) Multiplex sequencing method using primers of different lengths to detect polymorphisms
JP2622255B2 (ja) 択一的ヌクレオチド配列の識別方法
Binns et al. The identification of polymorphicmicrosatellite loci in the horse and their use in thoroughbred parentage testing
US5599675A (en) DNA sequencing by stepwise ligation and cleavage
EP0385410B1 (fr) Oligonucléotide à double brin partiel et son procédé de formation
US6514699B1 (en) Multiplex polynucleotide capture methods and compositions
US5639611A (en) Allele specific polymerase chain reaction
EP0705349B1 (fr) Approche d'analyse de sequences d'acides nucleiques par extension d'amorces parallele
EP0861332B1 (fr) Procede de sequenage
US5650274A (en) DNA analyzing method
EP0184056B1 (fr) Production d'ADN à grande échelle
JP3628614B2 (ja) 多重核酸検出のためのプローブ／移動度モディファイア複合体
EP0550693B1 (fr) Clonage direct d'acides nucleiques amplifies par reaction en chaine de la polymerase (pcr)
US5817797A (en) Sequencing DNA; a modification of the polymerase chain reaction
CN100408692C (zh) 筛选核酸以寻找核苷酸变异的方法
AU708678B2 (en) Sequencing of nucleic acids
WO1996030545A9 (fr) Detection d'une mutation par une extension differentielle d'amorce de sequences cibles mutantes et sauvages
US6528256B1 (en) Methods for identification and isolation of specific nucleotide sequences in cDNA and genomic DNA
EP1021559B1 (fr) Procede et trousse d'amplification, de sequencage et de typage de genes hla de classe 1 classiques
EP0648222B1 (fr) Procede d'allongement d'amorce de nucleotide simple brin destine a detecter des alleles specifiques et trousses permettant d'appliquer ledit procede
US5831065A (en) Kits for DNA sequencing by stepwise ligation and cleavage
AU660484B2 (en) Single step amplification and sequencing of nucleic acids
US20030082613A1 (en) Parallel primer extension approach to nucleic acid sequence analysis

Legal Events

Date Code Title Description
FZDE Dead