EP0595982A4 - Single step amplification and sequencing of nucleic acids - Google Patents

Single step amplification and sequencing of nucleic acids

Info

Publication number
EP0595982A4
EP0595982A4 EP92916411A EP92916411A EP0595982A4 EP 0595982 A4 EP0595982 A4 EP 0595982A4 EP 92916411 A EP92916411 A EP 92916411A EP 92916411 A EP92916411 A EP 92916411A EP 0595982 A4 EP0595982 A4 EP 0595982A4
Authority
EP
European Patent Office
Prior art keywords
nucleic acid
primer
strands
steps
primers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP92916411A
Other languages
English (en)
Other versions
EP0595982A1 (fr
Inventor
Keith Institute Of Biote Gregg
Christopher Lyle Cooper
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UNIVERSITY PARTNERSHIPS Pty Ltd
PARTNERSHIPS Pty Ltd, University of
Original Assignee
UNIVERSITY PARTNERSHIPS Pty Ltd
PARTNERSHIPS Pty Ltd, University of
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UNIVERSITY PARTNERSHIPS Pty Ltd, PARTNERSHIPS Pty Ltd, University of filed Critical UNIVERSITY PARTNERSHIPS Pty Ltd
Publication of EP0595982A1 publication Critical patent/EP0595982A1/fr
Publication of EP0595982A4 publication Critical patent/EP0595982A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Abstract

Procédé d'amplification et de sequençage d'ADN et d'ARN. Le procédé comprend les étapes suivantes: 1) à fusionner un acide nucléïque à double brin pour obtenir une paire de brins d'acide nucléïque complémentaires, 2) à hybrider une amorce à chacun des brins, les amorces étant choisies de telle sorte que la recircularisation de l'amorce sur le brin codant est en 3' par rapport à la position de l'amorce du brin non codant, une des amorces étant marquée de manière à être visualisée independamment de l'autre amorce, 3) à faire amplifier l'acide nucléïque à une polymerase en présence d'un analogue de didésoxynucléotide à l'un des nucléotides présents dans l'acide nucléïque, l'analogue de didésoxy étant présent dans une telle concentration que la majorité des brins d'acide nucléïque nouvellement synthétisés sont terminés par l'incorporation de didésoxynucléotides sans s'étendre assez loin pour agir comme gabarits de synthèse du brin opposé au moyen de la seconde amorce, 4) à répéter les étapes 1 à 3 séquentiellement un certain nombre de fois, 5) à répéter les étapes 1 à 4 en utilisant au moins deux autres analogues de didésoxynucléotides des autres 3 nucléotides présents dans l'acide nucléïque et 6) à séparer par électrophorèse les produits de réaction de chacune des répétitions des étapes 1 à 4 et à visualiser les brins marqués. L'autre des nucléotides d'au moins une partie du brin de l'acide nucléïque, sur lequel l'amorce marquée recircularisée entre les sites de liaison, peut être déterminé en comparant les gels séparés et visualisé pour chacun des analogues de nucléotides utilisés.
EP92916411A 1991-07-24 1992-07-23 Single step amplification and sequencing of nucleic acids Withdrawn EP0595982A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU7400/91 1991-07-24
AUPK740091 1991-07-24
PCT/AU1992/000372 WO1993002212A1 (fr) 1991-07-24 1992-07-23 Amplification et sequençage d'acides nucleiques en une etape

Publications (2)

Publication Number Publication Date
EP0595982A1 EP0595982A1 (fr) 1994-05-11
EP0595982A4 true EP0595982A4 (en) 1995-10-11

Family

ID=3775575

Family Applications (1)

Application Number Title Priority Date Filing Date
EP92916411A Withdrawn EP0595982A4 (en) 1991-07-24 1992-07-23 Single step amplification and sequencing of nucleic acids

Country Status (4)

Country Link
EP (1) EP0595982A4 (fr)
JP (1) JPH07500004A (fr)
CA (1) CA2114124A1 (fr)
WO (1) WO1993002212A1 (fr)

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US5480783A (en) * 1994-03-31 1996-01-02 The Perkin-Elmer Corporation Method for reducing background signals in DNA replication/detection assays
US6403303B1 (en) * 1996-05-14 2002-06-11 Visible Genetics Inc. Method and reagents for testing for mutations in the BRCA1 gene
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
EP0655506B1 (fr) * 1994-10-17 1996-09-18 President And Fellows Of Harvard College Polymérase d'ADN ayant les nucléotiden modifiées pour le site d'adhésion de l'ADN
US5948614A (en) * 1995-09-08 1999-09-07 Life Technologies, Inc. Cloned DNA polymerases from thermotoga maritima and mutants thereof
US5795722A (en) * 1997-03-18 1998-08-18 Visible Genetics Inc. Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
US5830657A (en) * 1996-05-01 1998-11-03 Visible Genetics Inc. Method for single-tube sequencing of nucleic acid polymers
US5888736A (en) * 1995-12-22 1999-03-30 Visible Genetics, Inc. Method, compositions and kit for detection and identification of microorganisms
US5789168A (en) * 1996-05-01 1998-08-04 Visible Genetics Inc. Method for amplification and sequencing of nucleic acid polymers
US6214555B1 (en) 1996-05-01 2001-04-10 Visible Genetics Inc. Method compositions and kit for detection
US6083699A (en) * 1996-05-01 2000-07-04 Visible Genetics Inc. Method for bi-directional sequencing of nucleic acid polymers
DE69709087T2 (de) * 1996-05-01 2002-04-25 Visible Genetics Inc Verfahren zum nachweis und zur identifizierung von mikroorganismen
US6413718B1 (en) 1996-05-01 2002-07-02 Visible Genetics Inc. Method for sequencing of nucleic acid polymers
US5928906A (en) * 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
EP0927268A1 (fr) * 1996-08-27 1999-07-07 Visible Genetics Inc. Appareil et procede pour la mise en oeuvre d'un sequen age de polymeres nucleotidiques
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
DE19653439A1 (de) * 1996-12-20 1998-07-02 Svante Dr Paeaebo Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung
DE19653494A1 (de) * 1996-12-20 1998-06-25 Svante Dr Paeaebo Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen unter Zugabe einer zweiten thermostabilen DNA Polymerase und dessen Anwendung
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
US6197557B1 (en) 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
US7270958B2 (en) 1998-09-10 2007-09-18 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20020187477A1 (en) 2001-06-06 2002-12-12 Hong Xue Method for detecting single nucleotide polymorphisms (SNPs) and point mutations
US7222059B2 (en) 2001-11-15 2007-05-22 Siemens Medical Solutions Diagnostics Electrophoretic trace simulator
ATE469984T1 (de) 2003-12-01 2010-06-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
DE102012201717A1 (de) * 2012-02-06 2013-08-08 AusBio Laboratories Co, Ltd. Probenträger-Zentrifuge

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0409078A2 (fr) * 1989-07-19 1991-01-23 BASF Aktiengesellschaft Procédé de séquence d'acides déoxyribonucléiques

Family Cites Families (5)

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Publication number Priority date Publication date Assignee Title
SE8801070D0 (sv) * 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
IL92474A (en) * 1988-11-29 1994-04-12 Orion Yhtymae Oy Reaction method and combination for determining nucleotide sequences
US5108892A (en) * 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
AU5675490A (en) * 1990-04-13 1991-11-11 Consejo Superior De Investigaciones Cientificas (in vitro) dna synthesis reactions using phi 29 dna polymerase and a dna fragment encoding said polymerase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0409078A2 (fr) * 1989-07-19 1991-01-23 BASF Aktiengesellschaft Procédé de séquence d'acides déoxyribonucléiques

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
D.B. OLSEN AND F. ECKSTEIN: "Incomplete primer extension during in vitro DNA amplification catalyzed by Taq polymerase; exploitation for DNA sequencing", NUCLEIC ACIDS RESEARCH, vol. 17, no. 21, 11 November 1989 (1989-11-11), IRL PRESS LIMITED,OXFORD,ENGLAND, pages 9613 - 9620 *
G. RUANO AND K.K. KIDD: "Coupled amplification and sequencing of genomic DNA", PROC. NATL.ACAD SCI., vol. 88, no. 7, 1 April 1991 (1991-04-01), NATL. ACAD SCI.,WASHINGTON,DC,US;, pages 2815 - 2819 *
K.L. NAKAMAYE ET AL.: "Direct sequencing of polymerase chain reaction amplified DNA fragments through the incorporation of deoxynucleoside alpha-triphosphates", NUCLEIC ACIDS RESEARCH, vol. 16, no. 21, IRL PRESS LIMITED,OXFORD,ENGLAND, pages 9947 - 9959 *
S.-J. DENG ET AL.: "Simultaneous amplification and sequencing of genomic DNA (SAS): sequencing of 16S rRNA genes using total genomic DNA from Butyrivibrio fibrisolvens, and detection and genotyping of nonculturable mycoplasma-like organisms directly from total DNA isolated from infected plants", J. MICROBIOL. METHODS, vol. 17, AMSTERDAM, NL, pages 103 - 113 *
See also references of WO9302212A1 *

Also Published As

Publication number Publication date
JPH07500004A (ja) 1995-01-05
EP0595982A1 (fr) 1994-05-11
WO1993002212A1 (fr) 1993-02-04
CA2114124A1 (fr) 1993-02-04

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