CA2110059A1 - Three highly informative microsatellite repeat polymorphic dna markers - Google Patents

Three highly informative microsatellite repeat polymorphic dna markers

Info

Publication number
CA2110059A1
CA2110059A1 CA002110059A CA2110059A CA2110059A1 CA 2110059 A1 CA2110059 A1 CA 2110059A1 CA 002110059 A CA002110059 A CA 002110059A CA 2110059 A CA2110059 A CA 2110059A CA 2110059 A1 CA2110059 A1 CA 2110059A1
Authority
CA
Canada
Prior art keywords
segments
markers
pcr
assay
differences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002110059A
Other languages
French (fr)
Other versions
CA2110059C (en
Inventor
Mihael H. Polymeropoulos
Carl R. Merrill
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Health and Human Services
Original Assignee
Mihael H. Polymeropoulos
Carl R. Merrill
The United States Of America, As Represented By The Secretary Of The Dep Artment Of Health And Human Services
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/799,828 external-priority patent/US5378602A/en
Application filed by Mihael H. Polymeropoulos, Carl R. Merrill, The United States Of America, As Represented By The Secretary Of The Dep Artment Of Health And Human Services filed Critical Mihael H. Polymeropoulos
Publication of CA2110059A1 publication Critical patent/CA2110059A1/en
Application granted granted Critical
Publication of CA2110059C publication Critical patent/CA2110059C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

The invention relates to polymorphic markers (two tetranucleotide, one dinucleotide repeat polymorphisms and 27 markers characterized by primer pairs 1A-27A) that are useful for human individualization. Applications are in forensic medicine and for paternity and prenatal screening as well as genetic mapping. These markers ae characterized by sets of oligonucleotide primers according to the invention useful in PCR amplification and DNA segment resolution. The invention further relates to an assay for measuring the subtle differences in genetic material regarding an added or omitted set of dinucleotide or tetranucleotide re-peat polymorphisms which comprises obtaining an amount of nucleotide segments effective for testing, amplifying the segments by the PCR procedure using at least one primer nucleotide sequence according to the present invention, resolving the amplified segments using gel electrophoresis, and comparing the resolved segments by autoradiography to observe the differences in migra-tion patterns due to structural differences. The assay according to the invention is easy to perform and results can be obtained within 24 hours. It is not uncommon for results to be available within 3-4 hours. Accordingly, the invention also relates to an im-proved PCR procedure and a PCR assay kit which comprise nucleotides according to the invention.
CA002110059A 1991-05-29 1992-05-27 Three highly informative microsatellite repeat polymorphic dna markers Expired - Lifetime CA2110059C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US70750191A 1991-05-29 1991-05-29
US707,501 1991-05-29
US07/799,828 US5378602A (en) 1991-05-29 1991-11-27 Highly informative microsatellite repeat polymorphic DNA markers twenty-[seven]six
US799,828 1991-11-27
PCT/US1992/004195 WO1992021693A1 (en) 1991-05-29 1992-05-27 Three highly informative microsatellite repeat polymorphic dna markers

Publications (2)

Publication Number Publication Date
CA2110059A1 true CA2110059A1 (en) 1992-12-10
CA2110059C CA2110059C (en) 2007-08-14

Family

ID=38434395

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002110059A Expired - Lifetime CA2110059C (en) 1991-05-29 1992-05-27 Three highly informative microsatellite repeat polymorphic dna markers

Country Status (1)

Country Link
CA (1) CA2110059C (en)

Also Published As

Publication number Publication date
CA2110059C (en) 2007-08-14

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