CA2086761A1 - Production of outer membrane (om) proteins in gram-positive bacteria and recovery of protective epitopes - Google Patents
Production of outer membrane (om) proteins in gram-positive bacteria and recovery of protective epitopesInfo
- Publication number
- CA2086761A1 CA2086761A1 CA002086761A CA2086761A CA2086761A1 CA 2086761 A1 CA2086761 A1 CA 2086761A1 CA 002086761 A CA002086761 A CA 002086761A CA 2086761 A CA2086761 A CA 2086761A CA 2086761 A1 CA2086761 A1 CA 2086761A1
- Authority
- CA
- Canada
- Prior art keywords
- outer membrane
- membrane protein
- protein
- gram
- cloned
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 210000002418 meninge Anatomy 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 210000004898 n-terminal fragment Anatomy 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 230000012846 protein folding Effects 0.000 description 1
- 108010009004 proteose-peptone Proteins 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 238000011552 rat model Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000013606 secretion vector Substances 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 229960004906 thiomersal Drugs 0.000 description 1
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- 238000005829 trimerization reaction Methods 0.000 description 1
- BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/22—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Neisseriaceae (F)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Public Health (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Communicable Diseases (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI903414A FI903414A (fi) | 1990-07-06 | 1990-07-06 | Produktion av proteiner i grampositiva bakterier. |
| FI903414 | 1990-07-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2086761A1 true CA2086761A1 (en) | 1992-01-07 |
Family
ID=8530757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002086761A Abandoned CA2086761A1 (en) | 1990-07-06 | 1991-07-05 | Production of outer membrane (om) proteins in gram-positive bacteria and recovery of protective epitopes |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0538318A1 (fi) |
| JP (1) | JPH06511140A (fi) |
| AU (2) | AU668075B2 (fi) |
| CA (1) | CA2086761A1 (fi) |
| FI (1) | FI903414A (fi) |
| IL (1) | IL98727A0 (fi) |
| WO (1) | WO1992001001A1 (fi) |
| ZA (1) | ZA915234B (fi) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6153406A (en) * | 1993-07-23 | 2000-11-28 | North American Vaccine, Inc. | Method for the high level expression, purification and refolding of the outer membrane protein P2 from Haemophilus influenzae type B |
| US5439808A (en) * | 1993-07-23 | 1995-08-08 | North American Vaccine, Inc. | Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis |
| DE4401419C1 (de) * | 1994-01-19 | 1994-12-22 | Forschungszentrum Juelich Gmbh | Sekretion von Proteinen der äußeren Membran Gram-negativer Bakterien mittels Gram-positiver Wirtsorganismen |
| US5747287A (en) * | 1995-04-28 | 1998-05-05 | North American Vaccine, Inc. | Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis |
| US5811102A (en) | 1995-06-07 | 1998-09-22 | National Research Council Of Canada | Modified meningococcal polysaccharide conjugate vaccines |
| SE9702191D0 (sv) * | 1997-06-09 | 1997-06-09 | Sven Pettersson | Coposition and methods for the treatment of inflammatory dideases |
| FR2785293B1 (fr) * | 1998-10-30 | 2002-07-05 | Pasteur Merieux Serums Vacc | Acides nucleiques et polypeptides specifiques des souches pathogenes du genre neisseria |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2534529B2 (ja) * | 1986-07-24 | 1996-09-18 | ブリティシュ・テレコミュニケ−ションズ・パブリック・リミテッド・カンパニ | 放射発生器 |
| NZ224065A (en) * | 1987-03-30 | 1991-02-26 | Texas A & M Univ Sys | Vaccine against brucella abortus |
-
1990
- 1990-07-06 FI FI903414A patent/FI903414A/fi not_active Application Discontinuation
-
1991
- 1991-07-04 IL IL98727A patent/IL98727A0/xx unknown
- 1991-07-05 AU AU81873/91A patent/AU668075B2/en not_active Expired - Fee Related
- 1991-07-05 EP EP91912587A patent/EP0538318A1/en not_active Withdrawn
- 1991-07-05 WO PCT/FI1991/000212 patent/WO1992001001A1/en not_active Application Discontinuation
- 1991-07-05 JP JP3511658A patent/JPH06511140A/ja active Pending
- 1991-07-05 CA CA002086761A patent/CA2086761A1/en not_active Abandoned
- 1991-07-05 ZA ZA915234A patent/ZA915234B/xx unknown
-
1996
- 1996-07-24 AU AU60692/96A patent/AU6069296A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| FI903414A (fi) | 1992-01-07 |
| EP0538318A1 (en) | 1993-04-28 |
| AU668075B2 (en) | 1996-04-26 |
| ZA915234B (en) | 1993-02-24 |
| AU6069296A (en) | 1996-11-07 |
| AU8187391A (en) | 1992-02-04 |
| JPH06511140A (ja) | 1994-12-15 |
| WO1992001001A1 (en) | 1992-01-23 |
| FI903414A0 (fi) | 1990-07-06 |
| IL98727A0 (en) | 1992-07-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |