CA2065439A1 - Expression de genes codant les cytochromes p450 pour la metabolisation des herbicides - Google Patents

Expression de genes codant les cytochromes p450 pour la metabolisation des herbicides

Info

Publication number
CA2065439A1
CA2065439A1 CA2065439A CA2065439A CA2065439A1 CA 2065439 A1 CA2065439 A1 CA 2065439A1 CA 2065439 A CA2065439 A CA 2065439A CA 2065439 A CA2065439 A CA 2065439A CA 2065439 A1 CA2065439 A1 CA 2065439A1
Authority
CA
Canada
Prior art keywords
metobolizing
cytochromes
herbicide
expression
enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2065439A
Other languages
English (en)
Other versions
CA2065439C (fr
Inventor
Caroline Dean
Patricia A. Harder
Kenneth J. Leto
Francis T. Lichtner, Jr.
Joan T. Odell
Daniel P. O'keefe
Charles A. Omer
James A. Romesser
Sandra H. Russell
James M. Tepperman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2065439A1 publication Critical patent/CA2065439A1/fr
Application granted granted Critical
Publication of CA2065439C publication Critical patent/CA2065439C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8267Seed dormancy, germination or sprouting
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8278Sulfonylurea
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289Male sterility
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cosmetics (AREA)
  • Saccharide Compounds (AREA)

Abstract

Des séquences ADN codant les enzymes de cytochrome P450 métabolisant les herbicides et des protéines ferro-sulfuriques qui donnent des électrons à ces enzymes, ont été introduites dans des plantes et des microorganismes et les ont rendus capables de produire les produits des gènes codés et de métaboliser les herbicides.
CA002065439A 1989-09-11 1990-08-27 Expression de genes codant les cytochromes p450 pour la metabolisation des herbicides Expired - Fee Related CA2065439C (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US40560589A 1989-09-11 1989-09-11
US07/405,605 1989-09-11
US46449990A 1990-01-12 1990-01-12
US07/464,499 1990-01-12
US07/569,781 1990-08-23
US07/569,781 US5212296A (en) 1989-09-11 1990-08-23 Expression of herbicide metabolizing cytochromes
PCT/US1990/004785 WO1991003561A1 (fr) 1989-09-11 1990-08-27 Expression des cytochromes p450 metabolisant les herbicides

Publications (2)

Publication Number Publication Date
CA2065439A1 true CA2065439A1 (fr) 1991-03-12
CA2065439C CA2065439C (fr) 2000-10-24

Family

ID=27410639

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002065439A Expired - Fee Related CA2065439C (fr) 1989-09-11 1990-08-27 Expression de genes codant les cytochromes p450 pour la metabolisation des herbicides

Country Status (11)

Country Link
US (2) US5212296A (fr)
EP (1) EP0554240B1 (fr)
JP (1) JP3206813B2 (fr)
AT (1) ATE133201T1 (fr)
AU (1) AU648036B2 (fr)
BR (1) BR9007656A (fr)
CA (1) CA2065439C (fr)
DE (1) DE69024979T2 (fr)
DK (1) DK0554240T3 (fr)
ES (1) ES2082862T3 (fr)
WO (1) WO1991003561A1 (fr)

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GB8610785D0 (en) * 1986-05-02 1986-07-09 Vickers Plc Attachment for armoured vehicle
US5420027A (en) * 1991-01-10 1995-05-30 Board Of Regents, The University Of Texas System Methods and compositions for the expression of biologically active fusion proteins comprising a eukaryotic cytochrome P450 fused to a reductase in bacteria
US5240831A (en) * 1991-01-10 1993-08-31 Board Of Regents, The University Of Texas Methods and compositions for the expression of biologically active eukaryotic cytochrome p45os in bacteria
GB9103314D0 (en) * 1991-02-16 1991-04-03 Imp Cancer Res Tech Assay systems
USRE36449E (en) * 1991-03-05 1999-12-14 Rhone-Poulenc Agro Chimeric gene for the transformation of plants
DE4126414A1 (de) * 1991-08-09 1993-02-11 Hoechst Ag Deacetylasegene zur erzeugung von phosphinothricin oder phosphinothricyl-alanyl-alanin, verfahren zu ihrer isolierung und ihre verwendung
CA2125947A1 (fr) * 1991-12-16 1993-06-24 Fateme Sima Sariaslani Expression constitutive de p450soy et de ferredoxine-soy dans les streptomyces et biotransformation de composes chimiques par des organismes recombinants
CA2138516A1 (fr) * 1992-06-25 1994-01-06 Hiroyuki Kawauchi Gene de la vitamine d - hydroxylase
GB9216151D0 (en) * 1992-07-29 1992-09-09 Ici Plc Containment of plant germplasm
US5409823A (en) * 1992-09-24 1995-04-25 Ciba-Geigy Corporation Methods for the production of hybrid seed
EP0628635A1 (fr) * 1993-06-08 1994-12-14 Nunhems Zaden Bv Procédé de préparation de plantes mâles stériles
WO1994029465A1 (fr) * 1993-06-08 1994-12-22 Nunhems Zaden B.V. Procede de generation de plantes males steriles
WO1995030745A1 (fr) * 1994-05-10 1995-11-16 Cornell Research Foundation, Inc. GENE DE CYTOCHROME P4501pr ET SES UTILISATIONS
US5824859A (en) * 1994-07-08 1998-10-20 The Trustees Of The University Of Pennsylvania Blue light photoreceptors and methods of using the same
US6121512A (en) * 1997-10-10 2000-09-19 North Carolina State University Cytochrome P-450 constructs and method of producing herbicide-resistant transgenic plants
US6054638A (en) * 1997-12-03 2000-04-25 E. I. Du Pont De Nemours And Company Soybean ADP ribosylation factor
AU4708799A (en) * 1998-06-26 2000-01-17 Board Of Trustees Of The University Of Illinois, The Maize cytochrome p450 monooxygenase cdna (cyp71c3v2)
US6380465B1 (en) * 1998-07-12 2002-04-30 University Of Kentucky Research Foundation Cytochrome P450 enzymes and related compounds and methods
AU5779499A (en) * 1998-08-28 2000-03-21 Pioneer Hi-Bred International, Inc. Organelle targeting sequences
CN1301759A (zh) * 1999-12-27 2001-07-04 上海博德基因开发有限公司 一种新的多肽——人铁硫蛋白10和编码这种多肽的多核苷酸
RU2532104C2 (ru) * 2001-10-19 2014-10-27 Сумитомо Кемикал Компани, Лимитед Метаболизирующий гербицид белок, его ген и их применение
DE10204843A1 (de) * 2002-02-06 2003-08-14 Gsf Forschungszentrum Umwelt Arabidopsis Thaliana-Array
JP4736480B2 (ja) 2004-05-17 2011-07-27 住友化学株式会社 雑草防除方法
JP4720223B2 (ja) * 2004-05-18 2011-07-13 住友化学株式会社 除草活性化合物耐性植物
US20060211553A1 (en) * 2005-03-16 2006-09-21 Cantor Zachary M Balance platform method and apparatus
CA2688682A1 (fr) * 2007-05-30 2008-12-11 Syngenta Participations Ag Genes de cytochrome p450 conferant une resistance aux herbicides
CN102348802B (zh) 2009-01-22 2014-09-24 先正达参股股份有限公司 突变体羟基苯丙酮酸双加氧酶多肽及其使用方法
CA2749383A1 (fr) 2009-07-10 2011-06-09 Syngenta Participations Ag Nouveaux polypeptides d'hydroxyphenylpyruvate dioxygenase et procedes d'utilisation
BR112012016787A2 (pt) 2010-01-07 2015-09-01 Basf Agro B V Arnhem Nl Wädenswil Branch "planta ou parte de planta, semente, célula de planta, produto de planta, progênie ou planta descendente derivada de uma planta, método para controlar ervas daninhas em um local para o desenvolvimento de uma planta, método para produzir e para tratar uma planta, para preparar e para tratar uma semente, e para produzir um produto de planta".
TWI667347B (zh) 2010-12-15 2019-08-01 瑞士商先正達合夥公司 大豆品種syht0h2及偵測其之組合物及方法
UY35702A (es) 2013-08-12 2015-02-27 Basf Se Hidroxifenilpiruvato dioxigenasas resistentes a herbicidas
AR103649A1 (es) 2015-02-11 2017-05-24 Basf Se Hidroxifenilpiruvato dioxigenasas resistentes a herbicidas
EA201892517A1 (ru) 2016-05-20 2019-05-31 Басф Агро Б.В. Двойные транзитные пептиды для нацеливания полипептидов
CN109790153A (zh) 2016-07-27 2019-05-21 巴斯夫农业公司 具有增加的除草剂耐受性的植物
UA127971C2 (uk) 2017-11-29 2024-02-28 Басф Се Спосіб контролю за небажаною рослинністю на ділянці вирощуванння рослини
WO2023031161A1 (fr) 2021-09-03 2023-03-09 BASF Agricultural Solutions Seed US LLC Plantes présentant une tolérance accrue aux herbicides

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US5169755A (en) * 1986-12-30 1992-12-08 E. I. Du Pont De Nemours And Company Process for inducing cytochrome P-450 enzymes in streptomyces bacteria and determining the mutagenicity of chemicals
AU605543B2 (en) * 1987-02-02 1991-01-17 Sankyo Company Limited Cytochrome p-450 enzymes
FI890917A (fi) * 1988-03-02 1989-09-03 Eidgenoess Tech Hochschule Foerfarande foer framstaellning av transgena vaexter.

Also Published As

Publication number Publication date
DK0554240T3 (da) 1996-06-03
DE69024979D1 (de) 1996-02-29
JP3206813B2 (ja) 2001-09-10
CA2065439C (fr) 2000-10-24
WO1991003561A1 (fr) 1991-03-21
AU6272990A (en) 1991-04-08
ATE133201T1 (de) 1996-02-15
AU648036B2 (en) 1994-04-14
EP0554240A1 (fr) 1993-08-11
US5349127A (en) 1994-09-20
EP0554240B1 (fr) 1996-01-17
DE69024979T2 (de) 1996-10-17
JPH05500002A (ja) 1993-01-14
ES2082862T3 (es) 1996-04-01
US5212296A (en) 1993-05-18
BR9007656A (pt) 1993-03-09

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