CA2065439A1 - Expression de genes codant les cytochromes p450 pour la metabolisation des herbicides - Google Patents

Expression de genes codant les cytochromes p450 pour la metabolisation des herbicides

Info

Publication number
CA2065439A1
CA2065439A1 CA2065439A CA2065439A CA2065439A1 CA 2065439 A1 CA2065439 A1 CA 2065439A1 CA 2065439 A CA2065439 A CA 2065439A CA 2065439 A CA2065439 A CA 2065439A CA 2065439 A1 CA2065439 A1 CA 2065439A1
Authority
CA
Canada
Prior art keywords
metobolizing
cytochromes
herbicide
expression
enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2065439A
Other languages
English (en)
Other versions
CA2065439C (fr
Inventor
Caroline Dean
Patricia A. Harder
Kenneth J. Leto
Francis T. Lichtner, Jr.
Joan T. Odell
Daniel P. O'keefe
Charles A. Omer
James A. Romesser
Sandra H. Russell
James M. Tepperman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2065439A1 publication Critical patent/CA2065439A1/fr
Application granted granted Critical
Publication of CA2065439C publication Critical patent/CA2065439C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8267Seed dormancy, germination or sprouting
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8278Sulfonylurea
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289Male sterility
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cosmetics (AREA)
  • Saccharide Compounds (AREA)

Abstract

Des séquences ADN codant les enzymes de cytochrome P450 métabolisant les herbicides et des protéines ferro-sulfuriques qui donnent des électrons à ces enzymes, ont été introduites dans des plantes et des microorganismes et les ont rendus capables de produire les produits des gènes codés et de métaboliser les herbicides.
CA002065439A 1989-09-11 1990-08-27 Expression de genes codant les cytochromes p450 pour la metabolisation des herbicides Expired - Fee Related CA2065439C (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US40560589A 1989-09-11 1989-09-11
US07/405,605 1989-09-11
US46449990A 1990-01-12 1990-01-12
US07/464,499 1990-01-12
US07/569,781 1990-08-23
US07/569,781 US5212296A (en) 1989-09-11 1990-08-23 Expression of herbicide metabolizing cytochromes
PCT/US1990/004785 WO1991003561A1 (fr) 1989-09-11 1990-08-27 Expression des cytochromes p450 metabolisant les herbicides

Publications (2)

Publication Number Publication Date
CA2065439A1 true CA2065439A1 (fr) 1991-03-12
CA2065439C CA2065439C (fr) 2000-10-24

Family

ID=27410639

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002065439A Expired - Fee Related CA2065439C (fr) 1989-09-11 1990-08-27 Expression de genes codant les cytochromes p450 pour la metabolisation des herbicides

Country Status (11)

Country Link
US (2) US5212296A (fr)
EP (1) EP0554240B1 (fr)
JP (1) JP3206813B2 (fr)
AT (1) ATE133201T1 (fr)
AU (1) AU648036B2 (fr)
BR (1) BR9007656A (fr)
CA (1) CA2065439C (fr)
DE (1) DE69024979T2 (fr)
DK (1) DK0554240T3 (fr)
ES (1) ES2082862T3 (fr)
WO (1) WO1991003561A1 (fr)

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GB8610785D0 (en) * 1986-05-02 1986-07-09 Vickers Plc Attachment for armoured vehicle
US5240831A (en) * 1991-01-10 1993-08-31 Board Of Regents, The University Of Texas Methods and compositions for the expression of biologically active eukaryotic cytochrome p45os in bacteria
US5420027A (en) * 1991-01-10 1995-05-30 Board Of Regents, The University Of Texas System Methods and compositions for the expression of biologically active fusion proteins comprising a eukaryotic cytochrome P450 fused to a reductase in bacteria
GB9103314D0 (en) * 1991-02-16 1991-04-03 Imp Cancer Res Tech Assay systems
USRE36449E (en) * 1991-03-05 1999-12-14 Rhone-Poulenc Agro Chimeric gene for the transformation of plants
DE4126414A1 (de) * 1991-08-09 1993-02-11 Hoechst Ag Deacetylasegene zur erzeugung von phosphinothricin oder phosphinothricyl-alanyl-alanin, verfahren zu ihrer isolierung und ihre verwendung
EP0618972A1 (fr) * 1991-12-16 1994-10-12 E.I. Du Pont De Nemours And Company EXPRESSION CONSTITUTIVE DE P450SOY ET FERREDOXINE-SOY CHEZ $i(STREPTOMYCES) ET BIOTRANSFORMATION DE PRODUITS CHIMIQUES PAR DES ORGANISMES RECOMBINES
WO1994000576A1 (fr) * 1992-06-25 1994-01-06 Taisho Pharmaceutical Co., Ltd. Gene hydroxylase de vitamine d
GB9216151D0 (en) * 1992-07-29 1992-09-09 Ici Plc Containment of plant germplasm
US5409823A (en) * 1992-09-24 1995-04-25 Ciba-Geigy Corporation Methods for the production of hybrid seed
EP0701619A1 (fr) * 1993-06-08 1996-03-20 Nunhems Zaden Bv Procede de generation de plantes males steriles
EP0628635A1 (fr) * 1993-06-08 1994-12-14 Nunhems Zaden Bv Procédé de préparation de plantes mâles stériles
WO1995030745A1 (fr) * 1994-05-10 1995-11-16 Cornell Research Foundation, Inc. GENE DE CYTOCHROME P4501pr ET SES UTILISATIONS
US5824859A (en) * 1994-07-08 1998-10-20 The Trustees Of The University Of Pennsylvania Blue light photoreceptors and methods of using the same
US6121512A (en) * 1997-10-10 2000-09-19 North Carolina State University Cytochrome P-450 constructs and method of producing herbicide-resistant transgenic plants
US6054638A (en) * 1997-12-03 2000-04-25 E. I. Du Pont De Nemours And Company Soybean ADP ribosylation factor
WO2000000502A1 (fr) * 1998-06-26 2000-01-06 The Board Of Trustees Of The University Of Illinois ADNc DE CYTOCHROME P450 MONOOXYGENASE DU MAIS (CYP71C3v2)
US6380465B1 (en) * 1998-07-12 2002-04-30 University Of Kentucky Research Foundation Cytochrome P450 enzymes and related compounds and methods
WO2000012732A2 (fr) * 1998-08-28 2000-03-09 Pioneer Hi-Bred International, Inc. Sequences de ciblage d'organelle
CN1301759A (zh) * 1999-12-27 2001-07-04 上海博德基因开发有限公司 一种新的多肽——人铁硫蛋白10和编码这种多肽的多核苷酸
BRPI0213411B1 (pt) * 2001-10-19 2015-11-03 Sumitomo Chemical Co dna codificando uma proteína de metabolização de herbicida, seu uso, vetor e micro-organismo transgênico compreendendo o mesmo, métodos de produção de um transformante, da referida proteína, de obtenção e detecção do referido dna e de identificação de uma célula compreendendo o referido dna
DE10204843A1 (de) * 2002-02-06 2003-08-14 Gsf Forschungszentrum Umwelt Arabidopsis Thaliana-Array
JP4736480B2 (ja) 2004-05-17 2011-07-27 住友化学株式会社 雑草防除方法
JP4720223B2 (ja) * 2004-05-18 2011-07-13 住友化学株式会社 除草活性化合物耐性植物
US20060211553A1 (en) * 2005-03-16 2006-09-21 Cantor Zachary M Balance platform method and apparatus
US8097774B2 (en) * 2007-05-30 2012-01-17 Syngenta Participations Ag Cytochrome P450 genes conferring herbicide resistance
WO2011068567A1 (fr) 2009-07-10 2011-06-09 Syngenta Participations Ag Nouveaux polypeptides d'hydroxyphénylpyruvate dioxygénase et procédés d'utilisation
CA2749524C (fr) 2009-01-22 2021-07-06 Syngenta Participations Ag Polypeptides de hydroxyphenylpyruvate dioxygenase mutante et procedes d'utilisation
EP2521441A4 (fr) 2010-01-07 2013-10-23 Basf Agro B V Arnhem Nl Zuerich Branch Plantes tolérantes aux herbicides
TWI667347B (zh) 2010-12-15 2019-08-01 瑞士商先正達合夥公司 大豆品種syht0h2及偵測其之組合物及方法
AR100017A1 (es) 2013-08-12 2016-09-07 Basf Se Hidroxifenilpiruvato dioxigenasas resistentes a herbicidas
US11248234B2 (en) 2015-02-11 2022-02-15 Basf Se Herbicide-resistant hydroxyphenylpyruvate dioxygenases
CR20180597A (es) 2016-05-20 2019-05-07 Basf Agro Bv Péptidos de tránsito dual para el direccionamiento a polipéptidos
WO2018019860A1 (fr) 2016-07-27 2018-02-01 BASF Agro B.V. Plantes ayant une tolérance accrue aux herbicides
UA127971C2 (uk) 2017-11-29 2024-02-28 Басф Се Спосіб контролю за небажаною рослинністю на ділянці вирощуванння рослини
AU2022340861A1 (en) 2021-09-03 2024-03-14 BASF Agricultural Solutions Seed US LLC Plants having increased tolerance to herbicides

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MX7065E (es) * 1980-06-06 1987-04-10 Sankyo Co Un procedimiento microbiologico para preparar derivados de ml-236b
US4720454A (en) * 1984-04-18 1988-01-19 White Perrin C Genetic probe used in the detection of adrenal hyperplasia
EP0215665B1 (fr) * 1985-09-13 1991-11-21 Sankyo Company Limited Dérivés de l'hydroxy-ML-236B, leur production et utilisation
JPS63162563A (ja) * 1986-12-25 1988-07-06 大日本インキ化学工業株式会社 セメント分散剤
US5169755A (en) * 1986-12-30 1992-12-08 E. I. Du Pont De Nemours And Company Process for inducing cytochrome P-450 enzymes in streptomyces bacteria and determining the mutagenicity of chemicals
DE3850589T2 (de) * 1987-02-02 1995-04-27 Sankyo Co Cytochrom-P-450-Enzyme.
EP0331083A3 (fr) * 1988-03-02 1990-11-28 Schweizerische Eidgenossenschaft Eidgenössische Technische Hochschule (Eth) Procédé pour la production de plantes transgéniques

Also Published As

Publication number Publication date
BR9007656A (pt) 1993-03-09
ATE133201T1 (de) 1996-02-15
ES2082862T3 (es) 1996-04-01
US5349127A (en) 1994-09-20
DK0554240T3 (da) 1996-06-03
DE69024979D1 (de) 1996-02-29
EP0554240B1 (fr) 1996-01-17
EP0554240A1 (fr) 1993-08-11
AU648036B2 (en) 1994-04-14
US5212296A (en) 1993-05-18
AU6272990A (en) 1991-04-08
JP3206813B2 (ja) 2001-09-10
WO1991003561A1 (fr) 1991-03-21
CA2065439C (fr) 2000-10-24
JPH05500002A (ja) 1993-01-14
DE69024979T2 (de) 1996-10-17

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