CA2044674A1 - Process for producing yogurt - Google Patents

Abstract

ABSTRACT

An improved process for producing yogurt which comprises adding highly purified whey proteins with a purity of at least 90% (by weight) to the raw material for production of yogurt or fermented products of said material in a ratio of at least 0.6%
(by weight) of their total protein content, whereby whey separation in the final product is effectively prevented and the final product having good texture and taste is obtained without injury to the concept of yogurt as a healthy food.

Description

20~7~

A PROCESS FOR PRO~UCING YOGURT

[FIELD OF THE INVENTION]
The presen~ invention relates to a process for producing yogurt. More particularly, the present invention relates to an improved process for producing yogurt which comprises adding highly purified whey proteins to the raw material for production of yogurt or fermented products of said material whereby whey separation in the final product is effectively prevented and the final product having good texture and taste is obtained.

[PRIOR ART]
In general, yogurt is paste-like or liquid, and made by fermenting animal milk or raw material for production of yogurt containing milk solids equal or more than those of anlmal milk with lactic acid bacteria and/or yeast. Depending upon the composition of the product, yogurt can be classified into, for example:
(1) Natural yogurt consisting only of animal milk components;

(2) Flavored yogurt with added chocolate, coffee extract, sugar, flavoring materials, or stabilizers;

(3) Fruit yogurt with fruit.
Yogurt can also be categorized, according to its properties and manufacturing processes, as, ~or example:
(1~ Solid type yogurt (set yogurt);
(2) Paste-like yogurt (stirred yogurt);

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(3) Liquid type yo~urt (drinking yoyur-t).
Ac-tually, in the food industry, b~ cornbining these compositions, properties, and manufacturing processes, many kinds of yogurt suiting the preferences largely held almost wholly in common by various age groups, from children -to adults to the aged, have been manufactured. However, it is not so simple or easy to produce delicious yogurt that has an appropriate solidity and viscosity, a smooth feel to the tongue, and that melts pleasantly in the mouth. What is more, for instance, one often sees yogurt in which, prior to the product's expiration date, the whey separation is observed, thereby its quality is seriously impaired. Thus, in order to solve these problems, it has been quite common practice (a) to add milk components such as skim milk powder, casein, or whey proteins with low purity; (b) to add thickeners such as sodium algina~e, gelatin, agar, or gum; or (c) to add milk components and thickeners. However, it is required in the above method (a) taht the addition of great amounts of milk components in order to obtain a sufficient effect, and the addition of such large amounts of milk components causes -the deterioration of taste in yogurt, and an increase in manufacturing costs. On the other hand, although an amount of addition of milk components in the above methods (b) and ~c) are lower than that of method (a), the concept as healthy food of the yogurt prepared by the above methods (b) and (c) is remarkabl~/ lost because of including additives other than milk components.
In view of the above prior art, the inventors of the .

present invention have made efforts to ~evelop a new process for producing yogurt which does no-t cause whey separation and does not contain in~redients other than milk components. As a result, the inventors discovered that whey separation ln the final product was effecti~ely prevented by addition of highly purified whey protelns to raw material for production of yogurt or fermented products of said raw material.

[SUMMARY OF THE INVENTION]
Accordingly, it is an aim of the present invention to provide an improved process for producing yogurt without whey separation which causes deterioration of quality in yogurt.
It is a further aim of the present invention to provide a yogurt without injury to the concept of yogurt as a healthy food.
The present invention relates to an improved process for producing yogurt which comprises adding highly purified whey proteins with a purity of at least 90 percent (by weight) (hereinafter referred to as percent for abbreviation) to the raw material for production of yogurt or fermented products of said material in a ratio of at least 0.6% of their total protein content, whereby whey separation in the final product is effectively prevented.

[DETAILED DESCRIPTION OF TEIE INVENTION]
The aforementioned highl~l purified whey proteins used in the present invention can be prepared, for example, by the 2 ~ 7 ~

following procedure.
The sweet whey or acid whey produced when making cheese or casein, the whey powder made by processiny them, whe~ protein concentrates, or any desired mix-tures of these (hereina~ter these shall all be referred to as "whey"), are contact with a weakly acidic cation exchanger having a carboxymethyl group, for example CM-Sephadex C-50 (trademark; sold by Pharmacia Co.~ or a weakly basic ion exchanger having a diaminoamylethyl group, for example D~AE-Sephadex A-50 (trademar~; sold by Pharmacia Co.), and the proteins in the whey are adsorbed with the ion exchanger. The ion exchanger is then separated and washed with water to completely remove the unadsorbed whey components.
Then, the ion exchanger is treated with aqueous solution of salt selected from a group consisting of sodium chloride, potassium chloride, magnesium chloride, and any desired mixture thereof, and the adsorbed protein is eluted from the ion exchanger.
The eluate is concentrated with an ultrafiltration device, and adjusted the salt content to 2 percent or less, thus the required highly purified whey proteins are obtained. The highly purified whey proteins can be used in the process for the present invention as the solution. The powdered form of highly purified whey proteins prepared by spray drying or freeze drying in order to prolong shelf life is also used in the present invention. The highly purified whey proteins have acceptable taste, and also have excellent gelling, whipping, and emulsifying properties.
Additionally, in using these highly purified whey proteins 2 ~ 7 ll for the present invention, their purity must be at least ~0 percent. In case of their purity is less than 90 percent, the sufficient effect in the final product cannot be obtained.
In producing yogurt with the process of the presen-t invention, highly purified whey proteins prepared by according to the aforementioned procedures are added either to the raw material for production of yogurt, or to fermented products of said raw material. When adding highly purified whey pro-teins to said raw material, powdered form of highly purified whey proteins are added and dissolved, while solution of highly purified whey proteins are added and mixed. When adding highly purified whey proteins to fermented products of said raw material, powdered form of highly purified whey proteins are added and mixed af~er dissolving them in water and solution of highly purified whey proteins are added and mixed with fermented products of said raw material. The amount of highly purified whey proteins added must be at least 0.6 percent of the total amount of proteins in the raw material for production of the yogurt or fermented products of said raw material, and preferably from 1.5 to 15.0 percent.
The form of the yogurt to which highly purified whey proteins are added with the process of the present invention may be solid, paste-like, or liquid, and the lactic acid bacteria used for fermentation may be of any kind, as long as they are used in the manufacture of fermented milk products. There is no limitation in particular on the species.

20~6 (Test 1) First the effec-t of the pur:ity of the whey proteins used with the present invention was tested.
1) Preparation of sample The compositon of the whey proteins used is showrl in table 1. Highly purified whey proteins were prepared by the same method as used in Example 1, while the MILACT~AL 75 (Trade name:
made by the Milei company of Germany), HIPROTAL (made by the Domo Co. of Holland), and whey powder (made by Morinaga), are all commercial products. MILACTEAL 75 and HIPROTAL are both manufactured ~y ultrafiltrating cheese whey to concentrate its whey proteins, and finally spray drying. The whey powder is produced by spray drylng cheese whey as is.
The raw material for production of yogurt was prepared adding powder form of highly purified whey proteins (0.3%), MILACTEAL 75 (0.36%), HIPROTAL (0.6%), and whey powder (2.25%) separately to each 1 kilogram of commercial milk (total protein content: 3%. The ratio of whey protein to the total protein in the raw material was in all cases, 9%). These mixtures were pasteurized or 10 minutes at 90~, yogurt starter CH-l (made by CHR.Hansen Laboratory) was added at rate of 0.01%, and mixed uniformly. Each lOOml of the mixture was poured into beakers with 120ml volume, and were covered with aluminum foil, then fermented for 3 hours at 42~ in an incubator to prepare four kinds of natural yogurt. To obtain a control sample, natural yogurt was also prepared by the above same method using same raw material without adding any additional ingredients.

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2) Experimental Method ~ ach yogurt sample was stored for 14 days at 5~ in a cooling room. Immediately a~ter manufacturiny, on days 4, ~, 10, and 14, to examine the effect of the addition of the whey proteins, each sample was subjected to organoleptic test on taste and visual observation of whey separation by 10 panelists (consisting of 5 men and 5 women).
The taste was rated on the following basis: good, fair, or poor, and the whey separation was rated on the basis noted on the margin of Table 2. The table shows evaluations by more than 80% of the panelists.
3) Results The resul-ts of the test are shown in Table 2. The taste of the control sample without adding whey proteins was evaluated as good even after they were stored for 14 days, but immediately after manufacturing, slight whey separation was observed. Whey separation of control sample steadily proceeded after 4th day, and on the 10th day yogurt had no merchandise value. The sample containing highly purified whey proteins on the other hand, still had good taste and no whey separation was observed after being stored for 14 days.
The taste of the sample containing MILACTEAL ?5 slightly deteriorated after 14 days of storage, whey separation was markedly observed, and its value as merchandise was lost. The evaluation of the samples with HIPROTAL and whey powder to prevent whey separation was no better than that of the control sample, and the taste of the sample prepared by adding whey - 2 ~

proteins with low purity quickly deteriorated.
These results indicate that diferences in both the purity of the whey proteins al~d their manufacturing procedure result in slight differences in the content of lactose, or the content and the type of ash and whey proteins which coe~ist in the products of these processes, and that i-t is not possible to prevent whey separation and produce good tasting yogurt even if the ratio of the whey protein to the total protein content of the raw material for production of yogurt is coordinated. In the case of adding 4 kinds of whey proteins to the fermented products the similar results were obtained. Accordingly, it is essential that the purity of highly purified whey proteins to be used in the present invention are at least 90 percent.
Next, the results of a experiment carried out to determine the optimum proportions of the highly purified whey proteins added to the raw material for production of yogurt, or fermented products of said raw material are described.
(Test 2) The effect of adding highly purified whey proteins to raw material for production of yogurt were tested.
(1) Preparation of samples Raw material for production of yogurt was prepared by adding powdered form of highly purified-whey proteins (purity of 90 percent), obtained by the same method as described below in Example 1, to lKg each of ordinary marketed milk (total protein amount of 3.0 percent) in the proportions of 0, 0.02, 0.05, 0.1, 0.2, 0.3, 0.4, and 0.5 percent (the proportions of highly - a -purified whey proteins to the total amount of proteins in the raw material were 0, 0.6, 1.5, 3.0, 6.0, 9.0, 12.0, and 15.0 percent, respectively), the raw materials were pasteurized at gO~ for 10 minutes, cooled to 42~, and O.O1 percent of the ~ogurt star~er CH-1 (sold b~ CHR.Hansen Laboratory) was added to each raw material and mixed uniformly. Each 100ml of thus obtained liquid mixture was poured into beakers with 120ml volume, were covered with aluminum foil, and fermented for three hours at 42~ in an incubator, thereby eight kinds of solid type natural ~ogurt samples were prepared.
(2) Experimental Method ~ ach sample was examined by the same method as used in Test 1 supra.
(3) Results The results of this test are shown in Table 3. The taste of all samples were acceptable after being preserved for 14 days, but the control sample to which highly purified whey proteins had not been added was already observed slight whey separation at immediately after manufacturing. Whey separation of control sample proceeded after the fourth day, and on the tenth day the yogurt no longer had value as merchandise.
On the other hand,in the case of samples to which the highly purified whey proteins had been added, when the added protein proportion was 0.6 percent of the total protein amount in the raw material, slight whey separation was observed on the first day, but even after preserving for 14 days, whey separation was not observed to the extent that the yo~urt would 2 ~

no longer have value as merchandise. A~d if the proportion added addition was at least 1.5 percent, no whey separation was observed in all samples even after 14 days of preservation.
(Test 3) Next, ano-ther experiment on the effect of adding highly purified whey proteins to fermented products was also carried out.
~ 1) Preparation of samples Nine kg of ordinary marketed milk (total protein content 3.0 percent) were pasteurized at 90C for 10 minutes, and cooled to 42~; 0.01 percent of the yogurt starter CH-1 (made by CHR.
Hansen Laboratory) was added and uniformly mixed. After fermenting this liquid mixture for 3 hours at 42~ in an incubator, it was sufficiently stirred and produced a paste-like natural yogurt. Each lkg of the yogurt was poured in~o beakers of 1.2 liter capacity. Eighteen percent of aqueous solution containing highly purified whey proteins (purity of 90~), which were prepared by the same method as in Example 1, were added to the yogurt in the ratios of 0, 0.1, 0.25, 0.50, 1.0, 1.5, 2.0, and 2.5 percent and mixed uniformly (the proportions of highly purified whey proteins to the total amount of proteins in the yogurt were 0, 0.6, 1.5, 3.0, 6.0, 9.0, 12.0, and 15.0 percent, respectively). Each lOOml of the mixture wa5 poured into beakers with 120ml volume, were covered with alminum foil, thereby eight kinds of yogurt samples were prepared.
(2) Experimental Method Each sample was examined by the same method as used in 2 ~ 7 l~

Test l supra.
(3) Results The results of -this tes-t are shown in Table 4. The taste of all samples were acceptable after being preserved for 14 days, but the control sample to which highly purifled whey proteins had not been added was already observed sligh-t whey separation at immediately af-ter manufacturing. Whey separation of control sample proceeded after the fourth day, and on the seventh day the yogurt no lon~er had value as merchandise. On the other hand, in the case of samples to which the highly purified whey proteins had been added, when the added protein proportion was 0.6 percent of the total protein amount in the yogurt, slight whey separation was observed on the fourth day, but even after preserving for 14 days, no whey separation was observed to the extent that the yogurt would no longer have value as merchandise. And if the proportion added was at least 1.5 percent, no whey separation was observed even after 14 days of preservation.
From the results of the foregoing two experiments tTest 2 and Test 3) it was comfirmed that the amount of highly purified whey proteins added, whether the proteins are added to the raw material for production of yogurt, or to fermented products of the raw material, should be at least 0.6 percent of the total protein content, and that 1.5 to 15.0 percent was preferable.

[EFFECTS OF THE INVENTION]
The effects of the present invention are as follows.

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(1) Whey sep.lration ln the fermented products can be effectively preventecl by using highly purif-ied whey proteins.
(2) The ~ogurt having good -texture, acceptable taste, and no whey separat.ion can be prepared without injury to the concept of yogurt as a healthy food.
(3) The value of whey proteins which were by-products of dairy plant in the past, can be remarkably enhanced.
The present invention will be illustrated b-y the following examples, but it is to be understood that the present invention not limi-ted to these examples.

Preparation of Highly Purified Whey Proteins Powder The pH of 16kg of acid whey (pH 4.6) was adjusted to 4.0 with diluted hydrochloric acid. Separately, 30g of the weakly acidic cation exchanger (CM-Sephadex C-50 (trade name; sold by Pharmacia Co.)) was swollen in warm water. To adsorbe whey protein with the ion exchanger, these were mixed and kept by stirring for 2 hours. The ion exchanger was then separated by using a container with a strainer of stainless steel. The ion exchanger was washed with water to remove unadsorbed whey components, and packed into a column. About 1500ml of a 5%
sodium chloride solution was passed through the column at a velocity of 2L/hrs. to elute the components which had been adsorbed with the ion exchanger. About 1500ml of eluate were obtained. The eluate was then ultrafiltrated by a filtration device (sold by Danish Sugar Corporation) equipped with an ultrafiltration membrane having a molecular weight cut-off of 20,000 at a circula-ting flow rate of aL/min. and an average pressure of 3k~/cm2. Water was then added to obtained permeate, and the sodium chloride frac-tion was removed by diafiltration, thereby approximately 2400ml of aqueous solution containing 18%
whey proteins were obtained. The a~ueous solution was freeze dried, thus about 40g of powder containing 90% whey proteins was obtained.

Producing Flavored Yogurt One hundred kg of skim milk (3.3% total protein), 8kg of sugar, 20g of vanilla flavoring, and 0.1kg of the highly purified whey proteins powder (purity of 90 percent) obtained by the same method as in Example 1 were put into a 150 liter fermenter and dissolved uniformly (the proportion of highly purified whey proteins to the total protein in the mixture was 2.7 percent). After pasteurizing for 10 minutes at 80~, the mixture was cooled to 42~, and then 0.01 percent yogurt starter CH-1 (sold by CHR.Hansen Laboratory) was added, and sufficiently mixed. The mixture was poured into 100ml yogurt cups in 90ml amounts, and fermented for three hours at 42~ in a incubation chamber to prepare solid type flavored yogurt. After fermentation was complete, the yogurt was stored at 5~ in a cooling room. Even after 14 days, the taste and texture of the yogurt were acceptable, and no whey separation was observed.

Producing Natural Yogurt One hundred kg of skim milk (3.3% total protein) and 0.3kg ~ 13 -2 ~ 7 ~

of the highly purified whey proteins powder ~purity of 90 percent) obtained by the same method as in ~xample 1 were put into a 150 liter fermerlter and dissolved uniformly (the propor-tion of highly purified whey proteins to the total protein in the mixture was 8.2 percent). After pasteurizing for 10 minu-tes at 80C, the mixture was cooled to 42~, and then o.O1 percent yogurt starter CH-1 (sold by CHR. Hansen Laboratory) was added, and the mixture was fermented for three hours. After fermentation was complete, the fermented product was sufficiently stirred while being cooled to 20C, and poured into lOOml yogurt cups in 90ml amounts. In this way, paste like natural yogurt was prepared and stored at 5~C in cooling room.
Even after 14 days, the taste and texture of the yogurt were acceptable, and no whey separation was observed.

Producing Fruit Yogurt One hundred kg of skim milk (3.3% total protein) and 3kg of sugar were put into a 150 liter fermenter and dissolved uniformly. After pasteurizing at 80C for 10 minutes, the mixture was cooled to 42~C, 0.01% yogurt starter CH-1 (sold by CHR. Hansen Laboratory~ was added, and the mixture was fermented for three hours. After fermentation was complete, 2kg of an aqueous solution containing 18 percent highly purified whey proteins obtained by the same method as in Example 1, and 5 kg of strawberry conserves were added, and cooled to 20C while mixing these in uniformly. The mixture was then poured into lOOml yogurt cups in 90ml amounts. In this way, paste-like 1 7 l~

fruit yogurt (the proportion of highly purified whey proteins to the total protein in the yoyurt was 10.9 percent) was prepared and stored at 5~ in a cooling roorn. Even after 14 days, the taste and texture of ~he yogur-t were acceptable, and no whey separation was observed.
EXAMPL~ 5 Preparation of Highly Pu_ified Whey Proteins The procedure of Example 1 was repeated except tha-t weakly basic ion exchanger (DEAE-Sephadex A-50 (trade mark; sold by Pharmacia Co.)) was used, and 40g of highly purified whey proteins obtained in powdered form were dissolved in 3000ml of water. Then the procedure of Example 1 was repeated in twice.
Thus about 30g of powder containing 92 percent of whey proteins was obtained.

Preparation of Natural Yogurt The procedure of Example 3 was repeated except that highly purified whey proteins prepared by the same procedure as used in Example 5 were used. Even after 14 days, the taste and texture of the obtained yogurt were acceptable, and no whey separation was observed.
Having now described the invention, it will be apparent to one of ordinary skill in the art that may changes had modifications can be made thereto without departing from -the spirit or scope of the invention as set forth herein.

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Claims

The embodiments of the invention in which an exclusive pro-perty or privilege is claimed are defined as follows:

(1) In a process for producing a yogurt, the improvement comprises adding highly purified whey proteins with a purity of at least 90 percent (by weight) to the raw material for production of yogurt or fermented products of said material in a ratio of at least 0.6 percent (by weight) of total protein content in said material or said products, whereby whey separation in the final product is effectively prevented.
(2) A process according to claim 1, wherein said highly purified whey proteins are prepared by the steps of;
(a) contacting a whey selected from group consisting of sweet whey, acid whey, whey powder, whey protein concentrate, and mixture thereof with a weakly acidic cation exchanger having carboxymenthyl group, or with weakly basic ion exchanger having a diaminoamylethyl group, to adsorbe the protein in the whey to the ion exchanger, (b) separating the ion exchanger from the whey, (c) washing the ion exchanger with water to remove unadsorbed whey components, (d) treating the ion exchanger with a salt solution to elute the whey proteins, (e) desalting and concentrating the eluat to obtain highly purified whey proteins.