CA2025079A1

CA2025079A1 - Bleaching wood pulp with enzymes

Info

Publication number: CA2025079A1
Application number: CA002025079A
Authority: CA
Inventors: Beat Gysin; Theophile Griessmann
Original assignee: Individual
Current assignee: Individual
Priority date: 1989-09-12
Filing date: 1990-09-11
Publication date: 1991-03-13
Also published as: NO903924L; PT95273A; NO178201C; JPH03104993A; NO903924D0; AU646403B2; DE69015294T2; FI904456A7; EP0418201A3; EP0418201B1; NO178201B; ES2067719T3; BR9004525A; EP0418201A2; AU6232390A; DE69015294D1; FI904456A0

Abstract

- 16 - Case 150-5361 Abstract Wood pulp may be delignified enzymatically with very good results when treating it with a lignin peroxidase in the absence of a peroxide and when the enzyme is firstly chemically modified in such a way that it does not adsorb to the pulp.

Description

2~2 ~rel~

- 1 - Case 150-5361 BLEAC~ING UOOD_PULP ~IT~ ~NZYH~S

This invention relates to a novel enzymatic process for bleaching wood pulp in which a crude lignin peroxidase is used in the presence of oxygen rather than hydrogen peroxide as co-substrate to reduce the lignin content of wood pulp. The lignin peroxidase can be used in a modified form.

Uood is a complex material which is composed of cellulose, hemi-cellulose and lignin along with other minor components. The lignin is associated with and even covalently bound to a matrix of cellulose and hemicellulose. In paper making processes, lignin should be removed from the wood pulp since it reduces the strength, confers a brownish colour and imparts other undesirable characteristics to the finished product. Conventionally, wood chips are first treated with sodium sulphide (Na2S) and sodium hydroxide (NaOH) to degrade the lignin substantially. Th;s is called the sulphate or Kraft process. Alterna-tively other treatments may be of use e.g. the sulphite process. The pulps obtained therefrom are called "chemical pulps".

Chemical pulp e.g~ Kraft pulp usually contains about 4-12~ by weight of residua:L lignin which gives the pulp a characteristic brown colour. At this stage of delignification, the kappa number which reflects the lignin content of the pulp is usually from 10 to 45, more frequently from 12 to 30. To obtain a pulp of high brightness and brightness stability, the lignin content should be further reduced in one or more treatments or stages commonly referred to as bleaching.
Many industrial bleaching processes already exist but almost all of them are divided into two main parts: A complementary delignification followed by a "true bleaching" for improving the brightness level. The complementary delignification typically starts with an oxygen stage or 7 ~ ~

- 2 - Case 150-5361 a chlorination-extraction step (C-E) stage or both. Chlorination and extraction are usually carried out in sequence, first forming chlori-nated lignin compounds which are then solubilized in the subsequent extraction step. The objective is exclusively to delignify the pulp as very little brightening occurs at the C-E stage. A complementary pro-cess for brightening the lignin may fureher include the use of compo-nents other than chlorine such as chlorine containing chemicals e.g.
hypochlorite and chlorine dioxide; or oxygen and hydrogen peroxide.

The effluents resulting from the complementary treatment (called E-1 effluents) contain a very large number of chlorinated organic compounds which are hazardous for the environment e.g. dioxines. Also, due to their highly corrosive nature, it is quite difficult to recycle the effluents. Thus, from the environmental point of view, it is clear that new techniques for bleaching which may reduce pollution are highly desirable.

In nature, there exist a number of microorganisms which delignify wood, and degrade and modify lignin. The enzymes involved in such a digestion belong to the classes of oxidases, peroxidases and hemi-cellulases. Thus, an enzymatic treatment may be usefully substituted for at least one of the chemical treatments involving chlorine com-pounds in pulp bleaching.

Lignin peroxidases (also called ligninases) and MnII-dependent peroxidase are enzymes of particular interest which are secreted by many microbial strains, especially filamentous fungi. Phanerochaete chrysosporium is a fungus which produces essentially both types of peroxidases. These enzymes are able to modify the lignin content of wood so that lignin is released from the hemicellulose matrix or made releasable upon washing or extraction.

However, the optimization of the experimental conditions in an enzymatic bleaching process has, however, not yet been achieved. This remains a major challenge since an en~ymatic process must be able to compete with a chemical process cn an industrial level.

r,~ ~

- 3 - Case 150-5361 Lignin peroxidases have been described up to now as enzymes which require the presence of H202 to be effective in degrading lignin with the optional presence of oxygen. In EP 345715 A1 it is claimed that this system works without the use of oxygen but in the presence of ~-hydroxy acids and detergents. At the same time it is claimed that the peroxide needs to be produced in situ enzymatically. In DE 3636208 A1 it is claimed that certain oxidation and reducing agents have to be present and the redox potential has to be maintained at a certain level throughout the course of the reaction. The processes described in these two patents are commercially not feasible because of the high costs of the co-substrates needed. In a recent publication (Holz-forschung 1989, 43(6), 375-384) it is shown that lignin peroxidases in the presence of hydrogen peroxide alone do not degrade lignin. In yet another publication (Enzyme Microbiol. Technol. 1985, 7(11), 564-566) it is shown that immobilized lignin peroxidases in combination with hydrogen peroxide alone do not delignify lignocellulosic material.

It has now been found that, surprisingly, very good results may be achieved in enzymatically delignifying wood pulp when treating the pulp with a lignin peroxidase in the absence of a peroxide and when the enzymes are firstly chemically modified in such a way that they do not adsorb to the pulp.

Thus, the invention provides a process for bleaching wood pulp which comprises treating the pulp with at least one lignin peroxidase in the substantial absence of added peroxide and in the presence of oxygen. In addition, the invention provides an enzymatic composition (enzyme preparation) comprising at least one lignin peroxidase derived from a fungal culture which is chemically modified so that it cannot be adsorbed onto pulp.

By "bleaching process" as used herein is meant a process for delignifying wood pulp or improving the whiteness or brightness of wood pulp or both.

By "lignin" as used herein is meant not only natural, unmodified forms but also the forms as found in chemically treated pulps which ~ 4 - Case 150-5361 are, in whole or in part, chemically modified by various agents such as those used in the Kraft, organosolv or sulphite pulping process and in the effluent of these processes.

By "substantial absence of added peroxide" is meant the absence of a substantial amount of added peroxide which would be effective in in-ducing degradation of lignin. Thus, the scope of the invention intends to encompass a process in which a peroxide is added to the reaction medium in an uneffective amount.

The term "lignin-degrading enzyme" as used herein is meant to en-compass any enzyme which modifies the lignin or hemicellulose compo-nent of wood so that lignin is released from the hemicellulose matrix or made releasable upon washing or extraction. Suitable lignin-degrading enzymes are hemicellulases, oxidases and peroxidases, the latter being particularly preferred. Examples of hemicellulases are mannanases, xylanases, galactomannanases and arabinosidases, while laccases fall under the group of oxidases. Preferred peroxidases are MnII-dependent peroxidases, and lignin peroxidases (also called ligninases). Lignin-degrading enzymes are secreted by many microbial strains particularly filamentous fungi.

The term "lignin peroxidases" as used herein is meant to encompass the crude enzyme preparation produced by the fungus under ligninolytic conditions as well as the individual lignin peroxidase isoenzymes from natural or recombinant producers.

Of preferred use is the lignin peroxidase of a white-rot fungus e.g. P. chrysosporium either from its native origin or in recombinant form. The recombinant form of a lignin peroxidase of P. chrysosporium may be obtained as described in PCT patent application No. 88/2023.

For use in the preferred process of the invention the enzymatic composition may be in a substantially purified form. It is, however, preferred that the enzymatic composition be a crude extract, a fil-trate or a supernatant of a culture of a white-rot fungus, e.g.
P. chrysosporium.

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- 5 - Case 150--5361 Strains of P. chrysosporium are publicly available and methods for culturing them in a N- or C-limited medium are already known. As an example, a suitable culture medium is the nitrogen-limited BIII/glu-cose medium which contains 1.08 x 10-3 M ammonium tartrate, 1.47 x 10-2 M KH2P04, 2.03 x 10_3 M MgS04 7H20, 6.8 x 10-4 M CaCl2-2H20, 2.96 x 10-6 M thiamine HCl and 10 ml L~1 of a trace element solution. The trace element solution contains 7.8 x 10-3 M nitrilo-acetic acid, 1.2 x 10-2 M MgS04-7H02, 1.7 x 10-2 M NaCl, 3.59 x 10- 4 M FeS04 7H20, 7.75 x 10-4 M CoCl2, 9.0 x 10-4 M CaCl2, 3.48 x 10-4 M ZnSOq, 4 x 10-5 M
CuS04 5H20, 2.1 x 10-5 M AlK(S04)z 12H20, 1.6 x 10-4 M H3B03, 4.1 x 0- 5 M NaMoO4 2H20 and 2.9 x 10-3 M MnS04 H20.

Eor use in the process of the invention, the lignin-degrading enzyme may be chemically modified by covalent or non-covalent linkage to water-soluble or insoluble polymeric compounds which prevent the enzyme from being adsorbed onto pulp during the treatment. Suitable polymeric compounds are for example, polyethylene glycol (PEG), poly-propylene glycol (PPG), polyacrylamides and polymeric sugars of various degrees of polymerization and composition like CM-cellulose, cellulose, agarose, alginate and chitosan. PEG is a preferred poly-meric compound.

Alternatively, the enzyme may be deglycosylated so that the carbohydrate residues which are usually involved in the mechanism of adsorption are at least partially removed. Deglycosylation may be performed by known methods, for example, by treating a sample of lignin-degrading enzyme with an enzyme such as an endoglycosidase capable of degrading carbohydrate residues on a glycoprotein.

The composition of the invention may be produced by chemical modi-fication of a crude extract, filtrate, or supernatant obtained from a fungal culture, preferably after concentration. Alternatively, the enzymes may be purified from a fungal material before any chemical treatment. It is particularly advantageous to use lignin peroxidases from a species or strain which does not produce cellulases especially ~2~

- 6 - Case 150-5361 when the enzyme is not purified. Of preferred use is the lignin perox-idase of a white-rot fungus, e.g. P. chrysosporium as indicated above.

The process of the invention may be applied to a wide variety of wood pulps the residual lignin content of which is to be reduced. Un-bleached wood pulps which may be treated with the process of the in-vention are advantageously mechanical pulps, e.g. groundwood pulp, including the thermomechanical pulps such as thermomechanical pulps (TMP), chemimechanical pulps (CMP), chemithermomechanical pulps (CTMP) and chemical pulps (CP) such as sulphite and Kraft pulps, these latter being preferred.

As a general rule, the enzyme concentration may range from 0.001 to 1000 VAO units/g pulp (a VAO unit is determined by the conversion of veratrylalcohol to veratrylaldehyde at 310 nm = 9.3 ~mol.cm~l at 30C, pH 3.5), preferably from 0.1 to 50 VAO units/g pulp, more preferably from 1 to 20 VAO units/g pulp. Optimal enzyme concentration depends upon the commercial origin and type of pulp.

Wood pulp is advantageously submitted to alkaline extraction be-fore being enzymatically treated. The enzymatic treatment is advanta-geously carried out at a pulp consistency of from 0.1 % to 15 %, pre-ferably of from 1 % to 5 %. The pulp consistency is determined by a standard procedure as the dry weight of pulp after drying for 2 to 10 hours at about 105C. To reach an optimal pulp consistency the un-bleached wood pulp may be diluted with deionized water, fresh water or tap water during the bleaching process. ~owever, for economical reasons, fresh water or tap water is preferred since it has been found that the characteristics of the water do not influence the final re-sults. By "fresh water" is meant water pumped directly e.g. from lakes, ponds or rivers.

It is preferable to wash the pulp with an alkaline solution before the enzymatic treatment or to perform the enzymatic treatment after an alkaline stage e.g. oxygen bleaching stage or E-stage.

- 7 - Case 150-5361 The period of time necessary for treating the pulp may greatly vary with respect to the quality of the substrate and the nature of the enzyme modification from a few minutes to several hours. Optimal temperature and p~ conditions should be adapted to the particular enzyme of use. However, temperature is generally in the range from 20 to 50C, preferably from 40 to 50 C. The pH of the system is usually in the range of from 2 to 5, preferably from 3 to 4. The reaction time is usually 30 to 60 minutes.

Following the enzymatic treatment, removal of the solubilized lignin from pulp may be carried out either by washing, filtration or by extraction, preferably by extraction. Suitable extractants include, for example, bases such as alkali metal hydroxides, dimethylformamide, dioxane, acetone and alcohol. A dilute aqueous sodium hydroxide ex-traction is generally preferred. A typical extraction step may be carried out at a pulp consistency from 1 to ~0%, preferably from 1 to 5% at a temperature between 40 and 60C. The final pH is preferably from 10 to 11. Reaction time may be from 30 minutes to 3 hrs, prefer-ably from 45 minutes to 2 hrs.

The extent of delignification of the pulp may be indicated by the Kappa number as measured in a standard method described in TAPPI Test Methods ~Tappi, Atlanta, Ga.) Vol. 1, 1988 "Kappa number of pulp - T
236 cm 85". The Kappa number is the volume (in millilitres) of ~.lN
potassium permanganate solution consumed by one gram of moisture-free pulp under the conditions specified in the above method. A lower Kappa number is desirable as it indicates that a smaller amount of lignin is present in the pulp.

Another similar process of particular interest involves also the treatment of aqueous waste water released from the pulping process of wood or from the bleaching process of wood pulp in order to further degrade the lignin component. A typical waste water which may be treated with a lignin peroxidase in the exclusive presence of oxygen as a co-substrate is the E1 effluent of the Kraft process.

The invention is further illustrated as follows:

- 8 - Case 150-5361 Example 1 Treatment of wood pulp with recombinant lignin peroxidase (ligninase) 1) Production of recombinant ligninase The recombinant apo-ligninase of P. chrysosporium is recovered from the pellet fraction of a culture lysate of E. coli (pBSR3) NRRL-18068 by extraction in 4Murea 50 mM sodium acetate lOmM
dithiothreitol (DTT). The supernatant extract is then separated from the pellet by appropriate centrifugation and applied on a DEAE-Sepharose anion exchange column. A gradient of O to lM NaCl is run in the extraction buffer. Fractions are collected and analysed for their immunoreactivity with an anti-ligninase antibody. The most strongly immunoreactive fractions are pooled and applied to a sizing column (S-300 Sephacryl; Pharmacia) in 4M urea 50mM KH2PO4 4mM DTT pH 7.
Again, the fractions are checked for their immunoreactivity and the most strongly reactive fractions are pooled and dialysed against Tris-HCl pH 8 lmM DTT 20% (v/v) glycerol.

Protoheme IX (Sigma) dissolved in O.lN KOH is added to the dialysed solution. This is then dialysed against 50m~ Tris HCl pH 8.
lmM reduced glutathione lOO~M oxidized glutathione overnight at 4C.
Finally the sample is dialysed against lOmM sodium acetate pH 6.

2) Bleaching process ~ith the recombinant ligninase 2.5 g of Kraft pulp obtained from hardwood are extracted first with 2.5% sodium hydroxide for one hour at 50C and then washed with tap water to neutrality. The consistency of the pulp is adjusted to 2.5% (approximately corresponding to 2.5 g pulp dîluted in 100 ml tap water) and the pH is lowered to pH 3.5 with hydrochloric acid. The mixture is then flushed with oxygen whilst stirring.

50 VAO units/g pulp of the reconstituted ligninase are added to the mixture and the reaction is performed for one hour at 40C. A
control sample with heat denatured enzyme is also prepared as well as a sample without enzyme.

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- 9 - Case 150-5361 The reaction is stopped by washing with tap water and the Kappa number of the enzymatically treated preparation and the control sample is measured. Surprisingly, the preparation treated with the recombi-nant ligninase in the exclusive presence of oxygen as co-substrate exhibits a lower Kappa number in comparison with the control sample, revealing that a significant delignification has been achieved.

Example 2 Treatment of wood pulp with a filtrate from a culture of P. chrysosporium A concentrated ligninolytic enæyme mixture essentially containing ligninases and Mn-dependent peroxidases is obtained by ultrafiltration (MU cut off 10,000) of a culture of P. chrysosporium ATCC 24 725 produced by the method of Linko, Enzyme Microb. Technol. 1988, 10, 410-417. Such a mixture has an enzymatic activity of 125 VAO units/ml.
The protein content of the mixture is 5 mg/ml as determined by the method of Bradford et al, Anal. Biochem. (1976) 72: 248.

7.5 g of Kraft pulp obtained from hardwood are extracted first with 2.5% sodium hydroxide for one hour at 50~C and then washed with tap water to neutrality. The consistency of the pulp is adjucted to 2.5% (approximately corresponding to 2.5 g pulp diluted in 100 ml tap water~ and the pH is lowered to pH 3.5 with hydrochloric acid.

The preparation is divided into three samples. One is supplemented with lOO~M H202, another one is supplemented with lOO~M H202 and flushed with oxygen, yet another one is only flushed with oxygen.

50 VAO units/g pulp of the enzymatic mixture are added to each sample and the reaction is performed for one hour at 40C whilst stirring. The reaction is stopped by washing with tap water and the Kappa number of the samples is measured.

Surprisingly, better results in delignifying the pulp are obtained in the exclusive presence of oxygen as a co-substrate than in the presence of hydrogen peroxide supplemented or not with oxygen.

- 10 - Case 150-53~1 Example 3 Modification of Enzyme Preparation Crude lignin peroxidase from Phanerochaete chrysosporium was either produced according to published procedures (e.g. H. Janshekar, A. Fiechter; J. of Biotechnology 1988, 8, 97-112) or purchased from Cultor Ltd., Helsinki; Finland.

a) hodification ~ith activated methoxypolyethylene glycol 1 ml of a crude lignin peroxidase preparation (activity: 110 VA0 units; protein content 5 mg (Bradford et al., Anal. Biochem. 1976, 72, 248)) was diluted in 9 ml acetate buffer 50 mM. After adjusting the pH
to 7.5, 1 g of cyanuric chloride activated methoxypolyethylene glycol (Sigma Nr. M-3277) was added. This solution was stirred over night at 4C. The enzymatic activity after the treatment was 75% of the origi-nal mixture. No further purification was carried out.

b) hodification wi~h ConA-Sepharose (Concanavalin A-Agarose) 1 ml of a crude lignin peroxidase preparation (as in a)) was mixed with 1 g of ConA-Sepharose (Pharmacia) in 10 ml acetate buffer (100 mM) at pH 7 overnight at 4C. The solid complex was then washed with 100 ml of the same buffer. The yield with respect to activity was 50%.

c) Deglycosylation 1 ml of a crude lignin peroxidase preparation (as in a)) was dil-uted in 1 ml acetate buffer (100 mM, pH 5). Then 10 units of endogly-cosidase F (Boehringer) are added to the preparation and the reaction is carried out at 37C for 2 hours. The yield with respect to activity was 30%-- 11 - Cas~ 150-5361 Example 4 Treatment of Wood Pulp 2.5 g of the appropriate pulp are extracted first with sodium hydroxide (2.5% of g dry pulp, 10% consistency) for one hour at 50C
and then washed with tap water to neutrality. After addition of 100 ml of tap water the pH is lowered to 3.5 with hydrochloric acid. The mixture is then flushed with oxygen whilst stirring.

The enzyme preparation prepared as described in Example 3 is then added to the pulp suspension and the reaction is then performed for one hour at 40C. The reaction is terminated by filtration and a sub-sequent sodium hydroxide extraction as described above.

The degree of delignification is measured by determination of the Kappa number. The lignin is also analytically detectable in the com-bined filtrate/alkaline extract e.g. by gel filtration high perfor-mance liquid chromatography using W/Vis spectroscopy for detection.

Better results in delignifying the pulp are obtained with a modi-fied enzyme preparation than with a non-modified enzyme preparation although the enzymatic activity of the modified preparation per g of pulp was 10 times lower than of the non-modified preparation (Table 1).

Better results are obtained when oxygen alone is used than when hydrogen peroxide alone is used (Table 2).

Delignification of hardwood kraft pulp, softwood kraft pulp, mixed mechanical pulp and softwood sulfite pulp can be achieved (Table 3).

~ ~ ~ 5'~ '~ ;'`f 9 - 12 - Case 150-5361 Table 1 Delignification of Hardwood Draft Pulp ¦ TYPE OF ¦ DELIGNIFICATION
¦ ENZYME PREPARATION¦ (~ Kappa Number Decrease)¦
.__ no enzyme O
PEG alone 2 ¦ ConA-Sepharose alone ¦ (a)PEG modified BSA, Heme ¦ (b)PEG modified, heat denatured 2 (b)PEG modified 15 (b)ConA modified 16 (c)recombinant 18 ¦ (c)non-modified 6 .. ... .. _ .~
All reaction mixtures were flushed with oxygen before and during the course of the reaction (1 hour).
a: PEG modified bovine serum albumine prepared in the same way as the modified enzyme preparation, bovine Heme (Sigma Nr. H-2250), 10 ~g/ml.
b: 5 VAO-units/g pulp.
c: 50 VAO-units/g pulp.
Table 2 Effect of Hydrogen Peroxide vs. Oxygen alone ._ ._ TYPE OF DELIGNIFICATION
ENZYME PREPARATION (% Kappa Number Decrease) __ ____ no enzyme O O
non-modified 50 units/g 0 6 recombinant 50 units/g 0 18 PEG-modified 5 units/g 0 15 ConA-modified 5 units/g 0 16 Hydrogen peroxide concentration was 100 ~Mol/litre; oxygen was as in Table 1.
Table 3 Delignification of Different Pulp Types . ~
PULP TYPEDELIGNIFICATION
(~ Kappa Number Decrease) . _ hardwood kraft 15 softwood kraft 8 mixed mechanical 4 softwood sulfite _ _ _ Enzyme concentration was 5 VAO-units/g pulp; A PEG-modified enzyme preparation was used.

- 13 - Gase 150-5361 Example 5 Treatment of Wood Pulp Example 4 is repeated using 50 VAO units~g pulp of the enzymatic mixture as prepared in Example 3 c). When added at the same concentra-tion, the enzymatic mixture treated with endoglycosidase F is more effective in delignifying the pulp than a non-modified mixture.

Example 6 Modification of Lignin 200 ~g Organosolv lignin (87/64003; Organocell, Munich BRD) from a 2% stock solution in dioxan in 1 ml of sodium tartrate buffer (100 mM) pH 3.5 were incubated with 1 VAO unit of ConA-Sepharose modified en-zyme preparation at 40C for one hour whilst flushed wi~h oxygen.
After that time the p~ was adjusted to 10.5 with sodium hydroxide and the sample was filtered through a 0.45 ~m filter to remove the enæyme/ConA complex. A sample treated in the same way with ConA-Sepharose but no enzyme was prepared at the same time.

The reaction products were analysed by gel permeation high perfor-mance liquid chromatography (HPLC) on two serially connected TSK (GMP
W&L, 7.8 x 300 mm) columns (Toya Soda, Japan). The flow rate was 1 ml/min. and sodium carbonate (10 mMt pH 10.5) with 0.05% polyethylene glycol (PEG 6000) was used as eluent. Absorption at 250, 310 and 360 nm was recorded using a diode array W-detector.

The enzyme treated lignin was extensively modified. Substantial brightening of the lignin suspension was observed after the enzyme treatment. The UV absorption spectra at 250~ 310 and 360 nm of the individual lignin components after separation by gel permeation chro-matography was extensively altered.

Claims

1. A process for bleaching wood pulp which comprises treating the pulp with at least one lignin peroxidase in the substantial absence of added peroxide and in the presence of added oxygen.

2. A process according to claim 1 in which the enzymatic composi-tion is selected from a crude extract, a filtrate or a supernatant of a white-rot fungus.

3. A process according to claim 2 in which the enzymatic composi-tion is selected from a crude extract, a filtrate or a supernatant of Phanerochaete chrysosporium.

4. A process according to claim 1 in which the lignin peroxidase is one obtained in recombinant form.

5. A process according to any one of claims 1 to 4 for bleaching Kraft pulp, organosolv or sulphite pulp.

6. A process for treating waste water released from the pulping treatment of wood or from the bleaching treatment of wood pulp which comprises treating the waste water with at least one lignin peroxidase in the absence of a peroxide and in the presence of oxygen.

7. A process according to claim 6 which comprises treating the waste water with an enzymatic composition which is selected from a crude extract, a filtrate or a supernatant of Phanerochaete chrysosporium.

8. A process according to claim 6 or 7 in which the waste water is the E1 effluent.

9. A process substantially as described in Example 1, 2, 4, 5 or 6.

10. An enzymatic composition for treating wood pulp comprising at least one lignin peroxidase derived from a fungus culture which is chemically modified so that it cannot be adsorbed onto the pulp.

- 15 - Case 150-5361

11. A composition according to claim 10 which is derived from a crude extract, a filtrate or a supernatant of a white-rot fungus culture.

12. A composition according to claim 11 in which the enzyme is a peroxidase from P.chrysosporium.

13. A composition according to any one of claims 10 to 12 in which the lignin peroxidase is associated with a water-soluble or insoluble polymeric compound by covalent or non-covalent linkage.

14. A composition according to claim 13 in which the soluble polymeric compound is selected from polyethylene glycol, polypropylene glycol, polymeric sugars and polyacrylamides.

15. A composition according to any one of claims 10 to 12 compri-sing a lignin peroxidase which is at least partially deglycosylated.

16. A composition substantially as described in Example 3.

17. A process for bleaching wood pulp which comprises treating the pulp with a composition according to any one of claims 10 to 16.

18. A process according to claim 17 in the substantial absence of added peroxide and in the presence of added oxygen.

19. A process for treating waste water released from the pulping treatment of wood or from the bleaching treatment of wood pulp which comprises treating the waste water with a composition according to any one of claims 10 to 16 in the absence of a peroxide and in the pre-sence of oxygen.

20. A process substantially as described in Example 4, 5 or 6.

6300/DH/Gro