CA2021643A1 - Methodes moleculaires pour la production de semences hybrides - Google Patents
Methodes moleculaires pour la production de semences hybridesInfo
- Publication number
- CA2021643A1 CA2021643A1 CA002021643A CA2021643A CA2021643A1 CA 2021643 A1 CA2021643 A1 CA 2021643A1 CA 002021643 A CA002021643 A CA 002021643A CA 2021643 A CA2021643 A CA 2021643A CA 2021643 A1 CA2021643 A1 CA 2021643A1
- Authority
- CA
- Canada
- Prior art keywords
- plant
- gene
- plants
- pollen
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 184
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 78
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 759
- 230000000692 anti-sense effect Effects 0.000 claims abstract description 50
- 108020004491 Antisense DNA Proteins 0.000 claims abstract 5
- 239000003816 antisense DNA Substances 0.000 claims abstract 5
- 241000196324 Embryophyta Species 0.000 claims description 1222
- 210000004027 cell Anatomy 0.000 claims description 395
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 291
- 108020004511 Recombinant DNA Proteins 0.000 claims description 207
- 230000006870 function Effects 0.000 claims description 204
- 102000053602 DNA Human genes 0.000 claims description 203
- 239000012634 fragment Substances 0.000 claims description 184
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 158
- 108020004414 DNA Proteins 0.000 claims description 153
- 230000015572 biosynthetic process Effects 0.000 claims description 147
- 238000013518 transcription Methods 0.000 claims description 133
- 230000035897 transcription Effects 0.000 claims description 133
- 239000002773 nucleotide Substances 0.000 claims description 108
- 125000003729 nucleotide group Chemical group 0.000 claims description 108
- 108091026890 Coding region Proteins 0.000 claims description 89
- 239000013043 chemical agent Substances 0.000 claims description 82
- 230000014509 gene expression Effects 0.000 claims description 68
- 230000009894 physiological stress Effects 0.000 claims description 62
- 210000000349 chromosome Anatomy 0.000 claims description 59
- 241000894007 species Species 0.000 claims description 52
- 230000000694 effects Effects 0.000 claims description 51
- 231100000433 cytotoxic Toxicity 0.000 claims description 50
- 230000001472 cytotoxic effect Effects 0.000 claims description 50
- 102000004169 proteins and genes Human genes 0.000 claims description 49
- 231100000252 nontoxic Toxicity 0.000 claims description 47
- 230000003000 nontoxic effect Effects 0.000 claims description 47
- 239000013612 plasmid Substances 0.000 claims description 47
- 210000001519 tissue Anatomy 0.000 claims description 45
- 238000011161 development Methods 0.000 claims description 43
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims description 39
- 230000001172 regenerating effect Effects 0.000 claims description 39
- 239000000126 substance Substances 0.000 claims description 39
- 239000003550 marker Substances 0.000 claims description 38
- 230000001939 inductive effect Effects 0.000 claims description 37
- 230000018109 developmental process Effects 0.000 claims description 36
- 239000003440 toxic substance Substances 0.000 claims description 31
- 239000003795 chemical substances by application Substances 0.000 claims description 30
- 230000035558 fertility Effects 0.000 claims description 30
- 230000001965 increasing effect Effects 0.000 claims description 29
- 229920001184 polypeptide Polymers 0.000 claims description 29
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 29
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 28
- 108010039491 Ricin Proteins 0.000 claims description 26
- 230000035882 stress Effects 0.000 claims description 26
- 241000219198 Brassica Species 0.000 claims description 21
- 230000036512 infertility Effects 0.000 claims description 21
- 238000002955 isolation Methods 0.000 claims description 21
- 239000000411 inducer Substances 0.000 claims description 20
- 230000008929 regeneration Effects 0.000 claims description 20
- 238000011069 regeneration method Methods 0.000 claims description 20
- 230000001105 regulatory effect Effects 0.000 claims description 19
- 235000011331 Brassica Nutrition 0.000 claims description 18
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 claims description 18
- 230000000295 complement effect Effects 0.000 claims description 16
- 108020004999 messenger RNA Proteins 0.000 claims description 14
- 230000010152 pollination Effects 0.000 claims description 13
- 230000002363 herbicidal effect Effects 0.000 claims description 12
- 239000004009 herbicide Substances 0.000 claims description 12
- 238000000338 in vitro Methods 0.000 claims description 12
- 230000002452 interceptive effect Effects 0.000 claims description 12
- 108010085238 Actins Proteins 0.000 claims description 11
- 102000007469 Actins Human genes 0.000 claims description 10
- AEMOLEFTQBMNLQ-AQKNRBDQSA-N D-glucopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-AQKNRBDQSA-N 0.000 claims description 10
- 230000010154 cross-pollination Effects 0.000 claims description 10
- ZOAMBXDOGPRZLP-UHFFFAOYSA-N indole-3-acetamide Chemical group C1=CC=C2C(CC(=O)N)=CNC2=C1 ZOAMBXDOGPRZLP-UHFFFAOYSA-N 0.000 claims description 10
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 claims description 9
- 230000003915 cell function Effects 0.000 claims description 9
- 229940097043 glucuronic acid Drugs 0.000 claims description 9
- 240000002791 Brassica napus Species 0.000 claims description 8
- 230000023715 cellular developmental process Effects 0.000 claims description 8
- 108700026220 vif Genes Proteins 0.000 claims description 8
- 108091005804 Peptidases Proteins 0.000 claims description 7
- 102000042567 non-coding RNA Human genes 0.000 claims description 7
- KFHRMMHGGBCRIV-UHFFFAOYSA-N 2-azaniumyl-4-methoxybutanoate Chemical compound COCCC(N)C(O)=O KFHRMMHGGBCRIV-UHFFFAOYSA-N 0.000 claims description 6
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 6
- 238000003306 harvesting Methods 0.000 claims description 6
- 108091027963 non-coding RNA Proteins 0.000 claims description 6
- 235000011293 Brassica napus Nutrition 0.000 claims description 5
- 108090000848 Ubiquitin Proteins 0.000 claims description 5
- 102000044159 Ubiquitin Human genes 0.000 claims description 5
- 230000009471 action Effects 0.000 claims description 5
- 229960005091 chloramphenicol Drugs 0.000 claims description 5
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 claims description 5
- 108010066676 Abrin Proteins 0.000 claims description 4
- 241000219193 Brassicaceae Species 0.000 claims description 4
- 239000004365 Protease Substances 0.000 claims description 4
- 238000004113 cell culture Methods 0.000 claims description 4
- 230000001131 transforming effect Effects 0.000 claims description 4
- 108010039918 Polylysine Proteins 0.000 claims description 3
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 229920000656 polylysine Polymers 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 230000001850 reproductive effect Effects 0.000 claims description 3
- 230000035939 shock Effects 0.000 claims description 3
- 239000003053 toxin Substances 0.000 claims description 3
- 231100000765 toxin Toxicity 0.000 claims description 3
- 108700012359 toxins Proteins 0.000 claims description 3
- SLUXPOIDTZWGCG-GONBATFASA-N (e,2s)-2-amino-4-[(2r)-2-amino-3-hydroxypropoxy]but-3-enoic acid Chemical compound OC[C@@H](N)CO\C=C\[C@H](N)C(O)=O SLUXPOIDTZWGCG-GONBATFASA-N 0.000 claims description 2
- XFNJVKMNNVCYEK-UHFFFAOYSA-N 1-naphthaleneacetamide Chemical group C1=CC=C2C(CC(=O)N)=CC=CC2=C1 XFNJVKMNNVCYEK-UHFFFAOYSA-N 0.000 claims description 2
- 102000053642 Catalytic RNA Human genes 0.000 claims description 2
- 108090000994 Catalytic RNA Proteins 0.000 claims description 2
- 239000004471 Glycine Substances 0.000 claims description 2
- 108010025815 Kanamycin Kinase Proteins 0.000 claims description 2
- 102000006382 Ribonucleases Human genes 0.000 claims description 2
- 108010083644 Ribonucleases Proteins 0.000 claims description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 claims description 2
- 108010012715 Superoxide dismutase Proteins 0.000 claims description 2
- 230000001010 compromised effect Effects 0.000 claims description 2
- 239000013600 plasmid vector Substances 0.000 claims description 2
- SLUXPOIDTZWGCG-UHFFFAOYSA-N rhizobitoxine Natural products OCC(N)COC=CC(N)C(O)=O SLUXPOIDTZWGCG-UHFFFAOYSA-N 0.000 claims description 2
- 108091092562 ribozyme Proteins 0.000 claims description 2
- 108700007698 Genetic Terminator Regions Proteins 0.000 claims 43
- 230000010165 autogamy Effects 0.000 claims 6
- 239000005562 Glyphosate Substances 0.000 claims 2
- 229940097068 glyphosate Drugs 0.000 claims 2
- 108010002685 hygromycin-B kinase Proteins 0.000 claims 2
- 108010079535 indoleacetamide hydrolase Proteins 0.000 claims 2
- 102000007698 Alcohol dehydrogenase Human genes 0.000 claims 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 claims 1
- 235000005637 Brassica campestris Nutrition 0.000 claims 1
- 241001301148 Brassica rapa subsp. oleifera Species 0.000 claims 1
- 239000005496 Chlorsulfuron Substances 0.000 claims 1
- 101710088194 Dehydrogenase Proteins 0.000 claims 1
- 102000016911 Deoxyribonucleases Human genes 0.000 claims 1
- 108010053770 Deoxyribonucleases Proteins 0.000 claims 1
- 102000004882 Lipase Human genes 0.000 claims 1
- 108090001060 Lipase Proteins 0.000 claims 1
- 239000004367 Lipase Substances 0.000 claims 1
- 241001599018 Melanogaster Species 0.000 claims 1
- 229920000037 Polyproline Polymers 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- VJYIFXVZLXQVHO-UHFFFAOYSA-N chlorsulfuron Chemical compound COC1=NC(C)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)Cl)=N1 VJYIFXVZLXQVHO-UHFFFAOYSA-N 0.000 claims 1
- 235000019421 lipase Nutrition 0.000 claims 1
- 108010026466 polyproline Proteins 0.000 claims 1
- 230000009466 transformation Effects 0.000 abstract description 26
- 230000008569 process Effects 0.000 abstract description 4
- 239000013598 vector Substances 0.000 description 170
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 69
- 150000002500 ions Chemical class 0.000 description 53
- 239000000047 product Substances 0.000 description 46
- 235000018102 proteins Nutrition 0.000 description 46
- 244000061176 Nicotiana tabacum Species 0.000 description 39
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 39
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 38
- 239000002299 complementary DNA Substances 0.000 description 37
- 108020004705 Codon Proteins 0.000 description 24
- 102000004190 Enzymes Human genes 0.000 description 24
- 108090000790 Enzymes Proteins 0.000 description 24
- 238000010276 construction Methods 0.000 description 20
- 235000012976 tarts Nutrition 0.000 description 19
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 18
- 231100000331 toxic Toxicity 0.000 description 16
- 230000002588 toxic effect Effects 0.000 description 16
- 101150062179 II gene Proteins 0.000 description 15
- 238000012217 deletion Methods 0.000 description 15
- 230000037430 deletion Effects 0.000 description 15
- 208000000509 infertility Diseases 0.000 description 14
- 208000021267 infertility disease Diseases 0.000 description 14
- 229930027917 kanamycin Natural products 0.000 description 14
- 229960000318 kanamycin Drugs 0.000 description 14
- 229930182823 kanamycin A Natural products 0.000 description 14
- 238000011144 upstream manufacturing Methods 0.000 description 14
- 206010021929 Infertility male Diseases 0.000 description 13
- 208000007466 Male Infertility Diseases 0.000 description 13
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 13
- 230000008488 polyadenylation Effects 0.000 description 13
- 101100536883 Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) thi5 gene Proteins 0.000 description 12
- 101100240664 Schizosaccharomyces pombe (strain 972 / ATCC 24843) nmt1 gene Proteins 0.000 description 12
- 230000001413 cellular effect Effects 0.000 description 11
- 108091081024 Start codon Proteins 0.000 description 10
- 238000010367 cloning Methods 0.000 description 10
- 239000000499 gel Substances 0.000 description 10
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 10
- 230000009467 reduction Effects 0.000 description 10
- 239000000523 sample Substances 0.000 description 10
- HLCHESOMJVGDSJ-UHFFFAOYSA-N thiq Chemical compound C1=CC(Cl)=CC=C1CC(C(=O)N1CCC(CN2N=CN=C2)(CC1)C1CCCCC1)NC(=O)C1NCC2=CC=CC=C2C1 HLCHESOMJVGDSJ-UHFFFAOYSA-N 0.000 description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
- 108700026226 TATA Box Proteins 0.000 description 9
- 239000002253 acid Substances 0.000 description 9
- 150000001413 amino acids Chemical class 0.000 description 9
- 229920001817 Agar Polymers 0.000 description 8
- 101100538857 Caenorhabditis elegans ttr-5 gene Proteins 0.000 description 8
- 238000002105 Southern blotting Methods 0.000 description 8
- 239000008272 agar Substances 0.000 description 8
- 230000033228 biological regulation Effects 0.000 description 8
- 239000002609 medium Substances 0.000 description 8
- 108091008146 restriction endonucleases Proteins 0.000 description 8
- 241000589158 Agrobacterium Species 0.000 description 7
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 7
- 238000013459 approach Methods 0.000 description 7
- 238000005520 cutting process Methods 0.000 description 7
- 230000002068 genetic effect Effects 0.000 description 7
- 101150054900 gus gene Proteins 0.000 description 7
- 238000003780 insertion Methods 0.000 description 7
- 230000037431 insertion Effects 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 230000036961 partial effect Effects 0.000 description 7
- 239000011734 sodium Substances 0.000 description 7
- 108020005544 Antisense RNA Proteins 0.000 description 6
- 241000518994 Conta Species 0.000 description 6
- 101150039033 Eci2 gene Proteins 0.000 description 6
- 101100181364 Mus musculus Lamb2 gene Proteins 0.000 description 6
- 102000035195 Peptidases Human genes 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 235000001014 amino acid Nutrition 0.000 description 6
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 239000003184 complementary RNA Substances 0.000 description 6
- 230000001086 cytosolic effect Effects 0.000 description 6
- 230000029087 digestion Effects 0.000 description 6
- 238000009396 hybridization Methods 0.000 description 6
- 102000004594 DNA Polymerase I Human genes 0.000 description 5
- 108010017826 DNA Polymerase I Proteins 0.000 description 5
- 238000000636 Northern blotting Methods 0.000 description 5
- 108700001094 Plant Genes Proteins 0.000 description 5
- 241000209140 Triticum Species 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 235000013339 cereals Nutrition 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 230000000875 corresponding effect Effects 0.000 description 5
- 238000010828 elution Methods 0.000 description 5
- 239000000284 extract Substances 0.000 description 5
- 239000003630 growth substance Substances 0.000 description 5
- 239000003617 indole-3-acetic acid Substances 0.000 description 5
- 230000005764 inhibitory process Effects 0.000 description 5
- 210000003470 mitochondria Anatomy 0.000 description 5
- 239000002674 ointment Substances 0.000 description 5
- 231100000590 oncogenic Toxicity 0.000 description 5
- 230000002246 oncogenic effect Effects 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- SGTNSNPWRIOYBX-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-{[2-(3,4-dimethoxyphenyl)ethyl](methyl)amino}-2-(propan-2-yl)pentanenitrile Chemical compound C1=C(OC)C(OC)=CC=C1CCN(C)CCCC(C#N)(C(C)C)C1=CC=C(OC)C(OC)=C1 SGTNSNPWRIOYBX-UHFFFAOYSA-N 0.000 description 4
- ODPOAESBSUKMHD-UHFFFAOYSA-L 6,7-dihydrodipyrido[1,2-b:1',2'-e]pyrazine-5,8-diium;dibromide Chemical compound [Br-].[Br-].C1=CC=[N+]2CC[N+]3=CC=CC=C3C2=C1 ODPOAESBSUKMHD-UHFFFAOYSA-L 0.000 description 4
- 239000005630 Diquat Substances 0.000 description 4
- 101000740205 Homo sapiens Sal-like protein 1 Proteins 0.000 description 4
- 102100037204 Sal-like protein 1 Human genes 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 102000004142 Trypsin Human genes 0.000 description 4
- 108090000631 Trypsin Proteins 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 229960001192 bekanamycin Drugs 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 239000013599 cloning vector Substances 0.000 description 4
- 229930182824 kanamycin B Natural products 0.000 description 4
- SKKLOUVUUNMCJE-FQSMHNGLSA-N kanamycin B Chemical compound N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SKKLOUVUUNMCJE-FQSMHNGLSA-N 0.000 description 4
- SKKLOUVUUNMCJE-UHFFFAOYSA-N kanendomycin Natural products NC1C(O)C(O)C(CN)OC1OC1C(O)C(OC2C(C(N)C(O)C(CO)O2)O)C(N)CC1N SKKLOUVUUNMCJE-UHFFFAOYSA-N 0.000 description 4
- 210000000056 organ Anatomy 0.000 description 4
- 239000011148 porous material Substances 0.000 description 4
- 230000005849 recognition of pollen Effects 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 3
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 3
- 235000006008 Brassica napus var napus Nutrition 0.000 description 3
- 240000000385 Brassica napus var. napus Species 0.000 description 3
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 3
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 3
- 108091033380 Coding strand Proteins 0.000 description 3
- IMXSCCDUAFEIOE-UHFFFAOYSA-N D-Octopin Natural products OC(=O)C(C)NC(C(O)=O)CCCN=C(N)N IMXSCCDUAFEIOE-UHFFFAOYSA-N 0.000 description 3
- IMXSCCDUAFEIOE-RITPCOANSA-N D-octopine Chemical compound [O-]C(=O)[C@@H](C)[NH2+][C@H](C([O-])=O)CCCNC(N)=[NH2+] IMXSCCDUAFEIOE-RITPCOANSA-N 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- 241000243190 Microsporidia Species 0.000 description 3
- UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 description 3
- 229930193140 Neomycin Natural products 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 108010068086 Polyubiquitin Proteins 0.000 description 3
- 108020004518 RNA Probes Proteins 0.000 description 3
- 239000003391 RNA probe Substances 0.000 description 3
- 235000003846 Ricinus Nutrition 0.000 description 3
- 241000322381 Ricinus <louse> Species 0.000 description 3
- 241000208292 Solanaceae Species 0.000 description 3
- 108020005038 Terminator Codon Proteins 0.000 description 3
- 241000209149 Zea Species 0.000 description 3
- 150000007513 acids Chemical class 0.000 description 3
- 230000009418 agronomic effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 229960003669 carbenicillin Drugs 0.000 description 3
- FPPNZSSZRUTDAP-UWFZAAFLSA-N carbenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)C(C(O)=O)C1=CC=CC=C1 FPPNZSSZRUTDAP-UWFZAAFLSA-N 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 125000001309 chloro group Chemical group Cl* 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 101150027734 cript gene Proteins 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 238000001502 gel electrophoresis Methods 0.000 description 3
- 230000002779 inactivation Effects 0.000 description 3
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 3
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 229960004927 neomycin Drugs 0.000 description 3
- 210000003463 organelle Anatomy 0.000 description 3
- 239000005648 plant growth regulator Substances 0.000 description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 3
- 235000019419 proteases Nutrition 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000005204 segregation Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 231100000167 toxic agent Toxicity 0.000 description 3
- 239000012588 trypsin Substances 0.000 description 3
- 230000005740 tumor formation Effects 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 3
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 description 2
- CXENHBSYCFFKJS-OXYODPPFSA-N (Z,E)-alpha-farnesene Chemical compound CC(C)=CCC\C(C)=C\C\C=C(\C)C=C CXENHBSYCFFKJS-OXYODPPFSA-N 0.000 description 2
- IVQOFBKHQCTVQV-UHFFFAOYSA-N 2-hydroxy-2,2-diphenylacetic acid 2-(diethylamino)ethyl ester Chemical compound C=1C=CC=CC=1C(O)(C(=O)OCCN(CC)CC)C1=CC=CC=C1 IVQOFBKHQCTVQV-UHFFFAOYSA-N 0.000 description 2
- BTJIUGUIPKRLHP-UHFFFAOYSA-N 4-nitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-N 0.000 description 2
- DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical compound CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 description 2
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 2
- 241000208838 Asteraceae Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 2
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 2
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 2
- 241000237074 Centris Species 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 108010053187 Diphtheria Toxin Proteins 0.000 description 2
- 102000016607 Diphtheria Toxin Human genes 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 108700024394 Exon Proteins 0.000 description 2
- 241000950314 Figura Species 0.000 description 2
- 108010060309 Glucuronidase Proteins 0.000 description 2
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 108091092195 Intron Proteins 0.000 description 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
- 101100128278 Mus musculus Lins1 gene Proteins 0.000 description 2
- 101100345589 Mus musculus Mical1 gene Proteins 0.000 description 2
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 241001282736 Oriens Species 0.000 description 2
- 241000209094 Oryza Species 0.000 description 2
- 240000007377 Petunia x hybrida Species 0.000 description 2
- 235000014676 Phragmites communis Nutrition 0.000 description 2
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 2
- 235000011613 Pinus brutia Nutrition 0.000 description 2
- 241000018646 Pinus brutia Species 0.000 description 2
- 241000219000 Populus Species 0.000 description 2
- 108091030071 RNAI Proteins 0.000 description 2
- 239000005708 Sodium hypochlorite Substances 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 101710162629 Trypsin inhibitor Proteins 0.000 description 2
- 229940122618 Trypsin inhibitor Drugs 0.000 description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
- 102000004243 Tubulin Human genes 0.000 description 2
- 108090000704 Tubulin Proteins 0.000 description 2
- 240000004543 Vicia ervilia Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 210000004899 c-terminal region Anatomy 0.000 description 2
- YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 239000003145 cytotoxic factor Substances 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 230000001066 destructive effect Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 210000004124 hock Anatomy 0.000 description 2
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 230000003050 macronutrient Effects 0.000 description 2
- 235000021073 macronutrients Nutrition 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 210000004379 membrane Anatomy 0.000 description 2
- 230000023409 microsporogenesis Effects 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 238000003976 plant breeding Methods 0.000 description 2
- 230000008119 pollen development Effects 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 235000019833 protease Nutrition 0.000 description 2
- 230000008707 rearrangement Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 239000002753 trypsin inhibitor Substances 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- BRZYSWJRSDMWLG-DJWUNRQOSA-N (2r,3r,4r,5r)-2-[(1s,2s,3r,4s,6r)-4,6-diamino-3-[(2s,3r,4r,5s,6r)-3-amino-4,5-dihydroxy-6-[(1r)-1-hydroxyethyl]oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol Chemical compound O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H]([C@@H](C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-DJWUNRQOSA-N 0.000 description 1
- WLNBMPZUVDTASE-HXIISURNSA-N (2r,3r,4s,5r)-2-amino-3,4,5,6-tetrahydroxyhexanal;sulfuric acid Chemical compound [O-]S([O-])(=O)=O.O=C[C@H]([NH3+])[C@@H](O)[C@H](O)[C@H](O)CO.O=C[C@H]([NH3+])[C@@H](O)[C@H](O)[C@H](O)CO WLNBMPZUVDTASE-HXIISURNSA-N 0.000 description 1
- AOFUBOWZWQFQJU-SNOJBQEQSA-N (2r,3s,4s,5r)-2,5-bis(hydroxymethyl)oxolane-2,3,4-triol;(2s,3r,4s,5s,6r)-6-(hydroxymethyl)oxane-2,3,4,5-tetrol Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O.OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O AOFUBOWZWQFQJU-SNOJBQEQSA-N 0.000 description 1
- 102100025230 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial Human genes 0.000 description 1
- SVTBMSDMJJWYQN-UHFFFAOYSA-N 2-methylpentane-2,4-diol Chemical compound CC(O)CC(C)(C)O SVTBMSDMJJWYQN-UHFFFAOYSA-N 0.000 description 1
- JGSARLDLIJGVTE-UHFFFAOYSA-N 3,3-dimethyl-7-oxo-6-[(2-phenylacetyl)amino]-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid Chemical compound O=C1N2C(C(O)=O)C(C)(C)SC2C1NC(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-UHFFFAOYSA-N 0.000 description 1
- 101150110188 30 gene Proteins 0.000 description 1
- BSFODEXXVBBYOC-UHFFFAOYSA-N 8-[4-(dimethylamino)butan-2-ylamino]quinolin-6-ol Chemical compound C1=CN=C2C(NC(CCN(C)C)C)=CC(O)=CC2=C1 BSFODEXXVBBYOC-UHFFFAOYSA-N 0.000 description 1
- 101150034533 ATIC gene Proteins 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 108010000700 Acetolactate synthase Proteins 0.000 description 1
- 108010087522 Aeromonas hydrophilia lipase-acyltransferase Proteins 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 101100099490 Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) thiY gene Proteins 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 241001136792 Alle Species 0.000 description 1
- 102000004118 Ammonia-Lyases Human genes 0.000 description 1
- 108090000673 Ammonia-Lyases Proteins 0.000 description 1
- 241000131859 Araotes Species 0.000 description 1
- 241000132092 Aster Species 0.000 description 1
- 235000021533 Beta vulgaris Nutrition 0.000 description 1
- 241000335053 Beta vulgaris Species 0.000 description 1
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
- 108010006654 Bleomycin Proteins 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 235000011303 Brassica alboglabra Nutrition 0.000 description 1
- 235000011302 Brassica oleracea Nutrition 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 1
- 235000012905 Brassica oleracea var viridis Nutrition 0.000 description 1
- 101100426970 Caenorhabditis elegans ttr-1 gene Proteins 0.000 description 1
- 101100426973 Caenorhabditis elegans ttr-3 gene Proteins 0.000 description 1
- 101100372800 Caenorhabditis elegans vit-2 gene Proteins 0.000 description 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- 241000905957 Channa melasoma Species 0.000 description 1
- 241000871189 Chenopodiaceae Species 0.000 description 1
- 102000012286 Chitinases Human genes 0.000 description 1
- 108010022172 Chitinases Proteins 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- LMKYZBGVKHTLTN-NKWVEPMBSA-N D-nopaline Chemical compound NC(=N)NCCC[C@@H](C(O)=O)N[C@@H](C(O)=O)CCC(O)=O LMKYZBGVKHTLTN-NKWVEPMBSA-N 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 1
- 241001057636 Dracaena deremensis Species 0.000 description 1
- 101100130497 Drosophila melanogaster Mical gene Proteins 0.000 description 1
- 101100285518 Drosophila melanogaster how gene Proteins 0.000 description 1
- 101100310856 Drosophila melanogaster spri gene Proteins 0.000 description 1
- 241001058146 Erium Species 0.000 description 1
- 241000620209 Escherichia coli DH5[alpha] Species 0.000 description 1
- 241000277301 Esociformes Species 0.000 description 1
- 241000220485 Fabaceae Species 0.000 description 1
- 244000228957 Ferula foetida Species 0.000 description 1
- 241000245654 Gladiolus Species 0.000 description 1
- 102000053187 Glucuronidase Human genes 0.000 description 1
- 241000720950 Gluta Species 0.000 description 1
- 101150087426 Gnal gene Proteins 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 101100244921 Homo sapiens PRDX1 gene Proteins 0.000 description 1
- 101150017040 I gene Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241000758791 Juglandaceae Species 0.000 description 1
- 240000007049 Juglans regia Species 0.000 description 1
- 235000009496 Juglans regia Nutrition 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- STECJAGHUSJQJN-USLFZFAMSA-N LSM-4015 Chemical compound C1([C@@H](CO)C(=O)OC2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-USLFZFAMSA-N 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 101710138460 Leaf protein Proteins 0.000 description 1
- 241000408529 Libra Species 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 241000219071 Malvaceae Species 0.000 description 1
- 108091027974 Mature messenger RNA Proteins 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 241001024304 Mino Species 0.000 description 1
- 101100118976 Mus musculus Clint1 gene Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108090000913 Nitrate Reductases Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108091092724 Noncoding DNA Proteins 0.000 description 1
- 108091093105 Nuclear DNA Proteins 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 101150072055 PAL1 gene Proteins 0.000 description 1
- 240000000220 Panda oleosa Species 0.000 description 1
- 235000016496 Panda oleosa Nutrition 0.000 description 1
- 241000199790 Paragus <genus> Species 0.000 description 1
- 102100029139 Peroxiredoxin-1 Human genes 0.000 description 1
- 241000312117 Phago Species 0.000 description 1
- WVHAUDNUGBNUDZ-SDQGTYQYSA-N Phenyl D-glucopyranosiduronic acid Chemical compound O1[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H](O)C1OC1=CC=CC=C1 WVHAUDNUGBNUDZ-SDQGTYQYSA-N 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 244000038538 Polygala oleifera Species 0.000 description 1
- 102100037935 Polyubiquitin-C Human genes 0.000 description 1
- RWCOTTLHDJWHRS-YUMQZZPRSA-N Pro-Pro Chemical compound OC(=O)[C@@H]1CCCN1C(=O)[C@H]1NCCC1 RWCOTTLHDJWHRS-YUMQZZPRSA-N 0.000 description 1
- 241001208007 Procas Species 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Chemical compound OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 101150064691 Q gene Proteins 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 241001632427 Radiola Species 0.000 description 1
- 101100355632 Rattus norvegicus Ran gene Proteins 0.000 description 1
- 101100313003 Rattus norvegicus Tanc1 gene Proteins 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 241000589180 Rhizobium Species 0.000 description 1
- 101710141795 Ribonuclease inhibitor Proteins 0.000 description 1
- 229940122208 Ribonuclease inhibitor Drugs 0.000 description 1
- 102100037968 Ribonuclease inhibitor Human genes 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 241000218998 Salicaceae Species 0.000 description 1
- 240000002625 Salsola soda Species 0.000 description 1
- 101100536893 Schizosaccharomyces pombe (strain 972 / ATCC 24843) thi9 gene Proteins 0.000 description 1
- 241000220261 Sinapis Species 0.000 description 1
- 241000207763 Solanum Species 0.000 description 1
- 235000002634 Solanum Nutrition 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 241000212346 Spermolepis Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 235000021536 Sugar beet Nutrition 0.000 description 1
- 241000950638 Symphysodon discus Species 0.000 description 1
- 101710137500 T7 RNA polymerase Proteins 0.000 description 1
- 206010057040 Temperature intolerance Diseases 0.000 description 1
- 241000011102 Thera Species 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 235000010726 Vigna sinensis Nutrition 0.000 description 1
- 244000042314 Vigna unguiculata Species 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000007152 anther development Effects 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 229960001561 bleomycin Drugs 0.000 description 1
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000000081 body of the sternum Anatomy 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 108091092356 cellular DNA Proteins 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 229930002868 chlorophyll a Natural products 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 229930002869 chlorophyll b Natural products 0.000 description 1
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 229960001338 colchicine Drugs 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000002844 continuous effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 101150074785 cut-3 gene Proteins 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000009585 enzyme analysis Methods 0.000 description 1
- 239000002532 enzyme inhibitor Substances 0.000 description 1
- 229940125532 enzyme inhibitor Drugs 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 108010052305 exodeoxyribonuclease III Proteins 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 108010060641 flavanone synthetase Proteins 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 230000008303 genetic mechanism Effects 0.000 description 1
- 229960004198 guanidine Drugs 0.000 description 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-O guanidinium Chemical compound NC(N)=[NH2+] ZRALSGWEFCBTJO-UHFFFAOYSA-O 0.000 description 1
- 101150118163 h gene Proteins 0.000 description 1
- 230000008543 heat sensitivity Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 208000006278 hypochromic anemia Diseases 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000010211 insect pollination Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- HOQADATXFBOEGG-UHFFFAOYSA-N isofenphos Chemical compound CCOP(=S)(NC(C)C)OC1=CC=CC=C1C(=O)OC(C)C HOQADATXFBOEGG-UHFFFAOYSA-N 0.000 description 1
- 101150085091 lat-2 gene Proteins 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000021121 meiosis Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000002923 metal particle Substances 0.000 description 1
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 1
- 229960000485 methotrexate Drugs 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 230000000394 mitotic effect Effects 0.000 description 1
- VYQNWZOUAUKGHI-UHFFFAOYSA-N monobenzone Chemical compound C1=CC(O)=CC=C1OCC1=CC=CC=C1 VYQNWZOUAUKGHI-UHFFFAOYSA-N 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- GWUSZQUVEVMBPI-UHFFFAOYSA-N nimetazepam Chemical compound N=1CC(=O)N(C)C2=CC=C([N+]([O-])=O)C=C2C=1C1=CC=CC=C1 GWUSZQUVEVMBPI-UHFFFAOYSA-N 0.000 description 1
- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000000574 octyl gallate Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- -1 oxygen radicals Chemical class 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- HCTVWSOKIJULET-LQDWTQKMSA-M phenoxymethylpenicillin potassium Chemical compound [K+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)COC1=CC=CC=C1 HCTVWSOKIJULET-LQDWTQKMSA-M 0.000 description 1
- WVHAUDNUGBNUDZ-UHFFFAOYSA-N phenyl beta-D-glucopyranosiduronic acid Natural products O1C(C(O)=O)C(O)C(O)C(O)C1OC1=CC=CC=C1 WVHAUDNUGBNUDZ-UHFFFAOYSA-N 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- SNWQKAWITMVCQW-UHFFFAOYSA-N phthalylsulfacetamide Chemical compound C1=CC(S(=O)(=O)NC(=O)C)=CC=C1NC(=O)C1=CC=CC=C1C(O)=O SNWQKAWITMVCQW-UHFFFAOYSA-N 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 230000000063 preceeding effect Effects 0.000 description 1
- 108010077112 prolyl-proline Proteins 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 230000010153 self-pollination Effects 0.000 description 1
- 230000002226 simultaneous effect Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 230000020347 spindle assembly Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- VLPFTAMPNXLGLX-UHFFFAOYSA-N trioctanoin Chemical compound CCCCCCCC(=O)OCC(OC(=O)CCCCCCC)COC(=O)CCCCCCC VLPFTAMPNXLGLX-UHFFFAOYSA-N 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 235000020234 walnut Nutrition 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002158333A CA2158333A1 (fr) | 1990-02-02 | 1990-07-20 | Methodes moleculaires pour la production de semences hybrides |
| CA002021643A CA2021643A1 (fr) | 1990-02-02 | 1990-07-20 | Methodes moleculaires pour la production de semences hybrides |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| WOPCT/CA90/00037 | 1990-02-02 | ||
| CA002021643A CA2021643A1 (fr) | 1990-02-02 | 1990-07-20 | Methodes moleculaires pour la production de semences hybrides |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002158333A Division CA2158333A1 (fr) | 1990-02-02 | 1990-07-20 | Methodes moleculaires pour la production de semences hybrides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2021643A1 true CA2021643A1 (fr) | 1992-01-21 |
Family
ID=4145535
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002021643A Abandoned CA2021643A1 (fr) | 1990-02-02 | 1990-07-20 | Methodes moleculaires pour la production de semences hybrides |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2021643A1 (fr) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996040950A1 (fr) * | 1995-06-07 | 1996-12-19 | Pioneer Hi-Bred International, Inc. | Procedes et produits d'assemblage de production de plantes males steriles |
| US5728926A (en) * | 1988-02-03 | 1998-03-17 | Pioneer Hi-Bred International, Inc. | Antisense gene systems of pollination control for hybrid seed production |
| US5728558A (en) * | 1988-02-03 | 1998-03-17 | Pioneer Hi-Bred International, Inc. | Molecular methods of hybrid seed production |
| US6162964A (en) * | 1988-02-03 | 2000-12-19 | Pioneer Hi-Bred International, Inc. | Molecular methods of hybrid seed production |
| US6184439B1 (en) | 1988-02-03 | 2001-02-06 | Pioneer Hi Bred International, Inc. | Antisense gene systems of pollination control for hybrid seed production |
| US7267964B2 (en) | 2000-03-31 | 2007-09-11 | Merial Limited | DNA molecules encoding ligand gated ion channels from Dermacentor variabilis |
-
1990
- 1990-07-20 CA CA002021643A patent/CA2021643A1/fr not_active Abandoned
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6184439B1 (en) | 1988-02-03 | 2001-02-06 | Pioneer Hi Bred International, Inc. | Antisense gene systems of pollination control for hybrid seed production |
| US6198023B1 (en) | 1988-02-03 | 2001-03-06 | Pioneer Hi-Bred International, Inc. | Molecular methods of hybrid seed production |
| US5728558A (en) * | 1988-02-03 | 1998-03-17 | Pioneer Hi-Bred International, Inc. | Molecular methods of hybrid seed production |
| US5741684A (en) * | 1988-02-03 | 1998-04-21 | Pioneer Hi-Bred International, Inc. | Molecular methods of hybrid seed production |
| US6191343B1 (en) | 1988-02-03 | 2001-02-20 | Pioneer Hi-Bred International | Molecular methods of hybrid seed production |
| US6162964A (en) * | 1988-02-03 | 2000-12-19 | Pioneer Hi-Bred International, Inc. | Molecular methods of hybrid seed production |
| US5728926A (en) * | 1988-02-03 | 1998-03-17 | Pioneer Hi-Bred International, Inc. | Antisense gene systems of pollination control for hybrid seed production |
| US6737560B1 (en) | 1988-02-03 | 2004-05-18 | Pioneer Hi-Bred International, Inc. | Molecular methods of hybrid seed production |
| US6255564B1 (en) | 1988-02-03 | 2001-07-03 | Paladin Hybrids, Inc. | Molecular methods of hybrid seed production |
| US6198026B1 (en) | 1988-02-03 | 2001-03-06 | Pioneer Hi-Bred International, Inc. | Molecular methods of hybrid seed production |
| US6013859A (en) * | 1994-07-14 | 2000-01-11 | Pioneer Hi-Bred International, Inc. | Molecular methods of hybrid seed production |
| WO1996040950A1 (fr) * | 1995-06-07 | 1996-12-19 | Pioneer Hi-Bred International, Inc. | Procedes et produits d'assemblage de production de plantes males steriles |
| US7267964B2 (en) | 2000-03-31 | 2007-09-11 | Merial Limited | DNA molecules encoding ligand gated ion channels from Dermacentor variabilis |
| US7655440B2 (en) | 2000-03-31 | 2010-02-02 | Merial Limited | DNA molecules encoding ligand gated ion channels from Dermacentor variabilis |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA1341346C (fr) | Systemes de controle de la pollinisation utilisant des genes antisens pour la production de semences hybrides | |
| EP0456706B1 (fr) | Procedes moleculaires de production de semences hybrides | |
| AU718262B2 (en) | Methods and constructs for producing male sterile plants | |
| US6184439B1 (en) | Antisense gene systems of pollination control for hybrid seed production | |
| KR100238904B1 (ko) | 재조합 식물 비루스 핵산 | |
| US5741684A (en) | Molecular methods of hybrid seed production | |
| CA2087703C (fr) | Methode cryptocytotoxique binaire pour la production de semences hybrides | |
| US6255564B1 (en) | Molecular methods of hybrid seed production | |
| RU1820914C (ru) | Способ получени двудольных растений, устойчивых к сульфонилмочевине | |
| Chatterjee et al. | DAG, a gene required for chloroplast differentiation and palisade development in Antirrhinum majus. | |
| JP2004524015A (ja) | ストレス耐性に関連するテンサイ遺伝子 | |
| US5728926A (en) | Antisense gene systems of pollination control for hybrid seed production | |
| JP2002532114A (ja) | トランスジェニック植物およびその作出法 | |
| CA2021643A1 (fr) | Methodes moleculaires pour la production de semences hybrides | |
| US8148603B2 (en) | Transgenic ficus, method for producing same and use thereof | |
| AU655574C (en) | Molecular methods of hybrid seed production | |
| EP1279734A1 (fr) | Procédés moléculaires de production de semences hybrides | |
| CA2158333A1 (fr) | Methodes moleculaires pour la production de semences hybrides |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Dead |