CA2010582C - Gene de tryptophane-decarboxylase produisant de la tryptamine d'origine vegetale - Google Patents
Gene de tryptophane-decarboxylase produisant de la tryptamine d'origine vegetaleInfo
- Publication number
- CA2010582C CA2010582C CA 2010582 CA2010582A CA2010582C CA 2010582 C CA2010582 C CA 2010582C CA 2010582 CA2010582 CA 2010582 CA 2010582 A CA2010582 A CA 2010582A CA 2010582 C CA2010582 C CA 2010582C
- Authority
- CA
- Canada
- Prior art keywords
- tryptophan decarboxylase
- tdc
- sequence
- coli
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000121 Aromatic-L-amino-acid decarboxylases Proteins 0.000 title claims abstract description 31
- APJYDQYYACXCRM-UHFFFAOYSA-N tryptamine Chemical compound C1=CC=C2C(CCN)=CNC2=C1 APJYDQYYACXCRM-UHFFFAOYSA-N 0.000 title description 9
- 102000003823 Aromatic-L-amino-acid decarboxylases Human genes 0.000 claims abstract description 27
- 240000001829 Catharanthus roseus Species 0.000 claims abstract description 19
- 241000196324 Embryophyta Species 0.000 claims description 33
- 108090000623 proteins and genes Proteins 0.000 claims description 25
- 241000588724 Escherichia coli Species 0.000 claims description 16
- 239000002773 nucleotide Substances 0.000 claims description 9
- 125000003729 nucleotide group Chemical group 0.000 claims description 9
- 108020004414 DNA Proteins 0.000 claims description 8
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 8
- 239000012634 fragment Substances 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004031 Carboxy-Lyases Human genes 0.000 claims description 5
- 108090000489 Carboxy-Lyases Proteins 0.000 claims description 5
- 239000013598 vector Substances 0.000 claims description 5
- 239000013604 expression vector Substances 0.000 claims 5
- 244000005700 microbiome Species 0.000 claims 5
- 241000894006 Bacteria Species 0.000 claims 2
- 102000053602 DNA Human genes 0.000 claims 2
- 108700005078 Synthetic Genes Proteins 0.000 claims 2
- 239000013612 plasmid Substances 0.000 claims 1
- 230000010076 replication Effects 0.000 claims 1
- 239000002299 complementary DNA Substances 0.000 abstract description 23
- 238000010367 cloning Methods 0.000 abstract description 2
- 238000011161 development Methods 0.000 abstract description 2
- 239000013600 plasmid vector Substances 0.000 abstract description 2
- 230000001131 transforming effect Effects 0.000 abstract description 2
- 238000002955 isolation Methods 0.000 abstract 1
- 101710096582 L-tyrosine decarboxylase Proteins 0.000 description 38
- 150000001413 amino acids Chemical class 0.000 description 22
- 235000018102 proteins Nutrition 0.000 description 18
- 102000004169 proteins and genes Human genes 0.000 description 18
- 229940024606 amino acid Drugs 0.000 description 12
- 235000001014 amino acid Nutrition 0.000 description 12
- 108010035075 Tyrosine decarboxylase Proteins 0.000 description 11
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 10
- 102100038238 Aromatic-L-amino-acid decarboxylase Human genes 0.000 description 10
- NGVDGCNFYWLIFO-UHFFFAOYSA-N pyridoxal 5'-phosphate Chemical compound CC1=NC=C(COP(O)(O)=O)C(C=O)=C1O NGVDGCNFYWLIFO-UHFFFAOYSA-N 0.000 description 10
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 9
- 239000000284 extract Substances 0.000 description 9
- 229960004799 tryptophan Drugs 0.000 description 9
- 108091034057 RNA (poly(A)) Proteins 0.000 description 7
- 239000000758 substrate Substances 0.000 description 7
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 6
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 6
- 241000863480 Vinca Species 0.000 description 6
- CJCSPKMFHVPWAR-JTQLQIEISA-N alpha-methyl-L-dopa Chemical compound OC(=O)[C@](N)(C)CC1=CC=C(O)C(O)=C1 CJCSPKMFHVPWAR-JTQLQIEISA-N 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- APJYDQYYACXCRM-UHFFFAOYSA-O tryptaminium Chemical compound C1=CC=C2C(CC[NH3+])=CNC2=C1 APJYDQYYACXCRM-UHFFFAOYSA-O 0.000 description 6
- 241000282324 Felis Species 0.000 description 5
- 102000008214 Glutamate decarboxylase Human genes 0.000 description 5
- 108091022930 Glutamate decarboxylase Proteins 0.000 description 5
- 241001599018 Melanogaster Species 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 230000002255 enzymatic effect Effects 0.000 description 5
- 108020004999 messenger RNA Proteins 0.000 description 5
- 235000007682 pyridoxal 5'-phosphate Nutrition 0.000 description 5
- 239000011589 pyridoxal 5'-phosphate Substances 0.000 description 5
- 229960001327 pyridoxal phosphate Drugs 0.000 description 5
- LDCYZAJDBXYCGN-VIFPVBQESA-N 5-hydroxy-L-tryptophan Chemical compound C1=C(O)C=C2C(C[C@H](N)C(O)=O)=CNC2=C1 LDCYZAJDBXYCGN-VIFPVBQESA-N 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 4
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000002243 precursor Substances 0.000 description 4
- 229960004441 tyrosine Drugs 0.000 description 4
- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 101001041236 Mus musculus Ornithine decarboxylase Proteins 0.000 description 3
- 238000012300 Sequence Analysis Methods 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229960005190 phenylalanine Drugs 0.000 description 3
- 229930001515 protoalkaloid Natural products 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- 108010044467 Isoenzymes Proteins 0.000 description 2
- WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 2
- 102000006335 Phosphate-Binding Proteins Human genes 0.000 description 2
- 108010058514 Phosphate-Binding Proteins Proteins 0.000 description 2
- RADKZDMFGJYCBB-UHFFFAOYSA-N Pyridoxal Chemical compound CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 238000000376 autoradiography Methods 0.000 description 2
- 229940083181 centrally acting adntiadrenergic agent methyldopa Drugs 0.000 description 2
- 230000001086 cytosolic effect Effects 0.000 description 2
- VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 238000002169 hydrotherapy Methods 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 239000002858 neurotransmitter agent Substances 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- LDCYZAJDBXYCGN-UHFFFAOYSA-N oxitriptan Natural products C1=C(O)C=C2C(CC(N)C(O)=O)=CNC2=C1 LDCYZAJDBXYCGN-UHFFFAOYSA-N 0.000 description 2
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- QZAYGJVTTNCVMB-UHFFFAOYSA-N serotonin Chemical compound C1=C(O)C=C2C(CCN)=CNC2=C1 QZAYGJVTTNCVMB-UHFFFAOYSA-N 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000004809 thin layer chromatography Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
- QIVBCDIJIAJPQS-HBGPJKQGSA-N (2S)-2-amino-3-(1H-indol-3-yl)(214C)propanoic acid Chemical compound N[14C@@H](CC1=CNC2=CC=CC=C12)C(=O)O QIVBCDIJIAJPQS-HBGPJKQGSA-N 0.000 description 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- APJYDQYYACXCRM-ZQBYOMGUSA-N 2-(1H-indol-3-yl)(114C)ethanamine Chemical compound N[14CH2]CC1=CNC2=CC=CC=C12 APJYDQYYACXCRM-ZQBYOMGUSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- 101710113919 3,4-dihydroxyphenylacetaldehyde synthase 2 Proteins 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 108020005350 Initiator Codon Proteins 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- KVOFSTUWVSQMDK-KKUMJFAQSA-N Leu-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC(C)C)CC1=CN=CN1 KVOFSTUWVSQMDK-KKUMJFAQSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 108091036407 Polyadenylation Proteins 0.000 description 1
- BAKAHWWRCCUDAF-IHRRRGAJSA-N Pro-His-Lys Chemical compound C([C@@H](C(=O)N[C@@H](CCCCN)C(O)=O)NC(=O)[C@H]1NCCC1)C1=CN=CN1 BAKAHWWRCCUDAF-IHRRRGAJSA-N 0.000 description 1
- 108010076504 Protein Sorting Signals Proteins 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 101000884043 Sus scrofa Aromatic-L-amino-acid decarboxylase Proteins 0.000 description 1
- 241001104043 Syringa Species 0.000 description 1
- 101150067147 TDC gene Proteins 0.000 description 1
- 241000205578 Thalictrum Species 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- JXLYSJRDGCGARV-WWYNWVTFSA-N Vinblastine Natural products O=C(O[C@H]1[C@](O)(C(=O)OC)[C@@H]2N(C)c3c(cc(c(OC)c3)[C@]3(C(=O)OC)c4[nH]c5c(c4CCN4C[C@](O)(CC)C[C@H](C3)C4)cccc5)[C@@]32[C@H]2[C@@]1(CC)C=CCN2CC3)C JXLYSJRDGCGARV-WWYNWVTFSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 229940060532 allent Drugs 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
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- 125000003118 aryl group Chemical group 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
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- SRGKFVAASLQVBO-BTJKTKAUSA-N brompheniramine maleate Chemical compound OC(=O)\C=C/C(O)=O.C=1C=CC=NC=1C(CCN(C)C)C1=CC=C(Br)C=C1 SRGKFVAASLQVBO-BTJKTKAUSA-N 0.000 description 1
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- WORJEOGGNQDSOE-UHFFFAOYSA-N chloroform;methanol Chemical compound OC.ClC(Cl)Cl WORJEOGGNQDSOE-UHFFFAOYSA-N 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
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- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229960003638 dopamine Drugs 0.000 description 1
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- 230000009088 enzymatic function Effects 0.000 description 1
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- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- DNDNWOWHUWNBCK-NMIPTCLMSA-N indolylmethylglucosinolate Chemical class O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1S\C(=N\OS(O)(=O)=O)CC1=CNC2=CC=CC=C12 DNDNWOWHUWNBCK-NMIPTCLMSA-N 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 229960004502 levodopa Drugs 0.000 description 1
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 125000000896 monocarboxylic acid group Chemical group 0.000 description 1
- 229930014716 monoterpenoid indole alkaloid Natural products 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 229960003581 pyridoxal Drugs 0.000 description 1
- 235000008164 pyridoxal Nutrition 0.000 description 1
- 239000011674 pyridoxal Substances 0.000 description 1
- 150000003230 pyrimidines Chemical class 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 238000011451 sequencing strategy Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 229940076279 serotonin Drugs 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 229960003048 vinblastine Drugs 0.000 description 1
- JXLYSJRDGCGARV-XQKSVPLYSA-N vincaleukoblastine Chemical compound C([C@@H](C[C@]1(C(=O)OC)C=2C(=CC3=C([C@]45[C@H]([C@@]([C@H](OC(C)=O)[C@]6(CC)C=CCN([C@H]56)CC4)(O)C(=O)OC)N3C)C=2)OC)C[C@@](C2)(O)CC)N2CCC2=C1NC1=CC=CC=C21 JXLYSJRDGCGARV-XQKSVPLYSA-N 0.000 description 1
- 229960004528 vincristine Drugs 0.000 description 1
- OGWKCGZFUXNPDA-XQKSVPLYSA-N vincristine Chemical compound C([N@]1C[C@@H](C[C@]2(C(=O)OC)C=3C(=CC4=C([C@]56[C@H]([C@@]([C@H](OC(C)=O)[C@]7(CC)C=CCN([C@H]67)CC5)(O)C(=O)OC)N4C=O)C=3)OC)C[C@@](C1)(O)CC)CC1=C2NC2=CC=CC=C12 OGWKCGZFUXNPDA-XQKSVPLYSA-N 0.000 description 1
- OGWKCGZFUXNPDA-UHFFFAOYSA-N vincristine Natural products C1C(CC)(O)CC(CC2(C(=O)OC)C=3C(=CC4=C(C56C(C(C(OC(C)=O)C7(CC)C=CCN(C67)CC5)(O)C(=O)OC)N4C=O)C=3)OC)CN1CCC1=C2NC2=CC=CC=C12 OGWKCGZFUXNPDA-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US31487989A | 1989-02-24 | 1989-02-24 | |
| US314,879 | 1989-02-24 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2010582A1 CA2010582A1 (fr) | 1990-08-24 |
| CA2010582C true CA2010582C (fr) | 1998-07-28 |
Family
ID=23221876
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2010582 Expired - Lifetime CA2010582C (fr) | 1989-02-24 | 1990-02-21 | Gene de tryptophane-decarboxylase produisant de la tryptamine d'origine vegetale |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0425597A1 (fr) |
| JP (1) | JP2919069B2 (fr) |
| CA (1) | CA2010582C (fr) |
| WO (1) | WO1990010073A1 (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5814520A (en) * | 1989-02-24 | 1998-09-29 | National Research Council Canada | Tryptamine producing tryptophan decarboxylase gene of plant origin |
| US7119262B1 (en) | 1997-07-31 | 2006-10-10 | Sanford Scientific, Inc. | Production of transgenic poinsettia |
| AU8682198A (en) * | 1997-07-31 | 1999-02-22 | Sanford Scientific, Inc. | Transgenic plants using the tdc gene for crop improvement |
| CN105274083B (zh) * | 2015-11-20 | 2018-11-23 | 中国科学院华南植物园 | 一种谷氨酸脱羧酶及其编码基因和应用 |
| CN113403351B (zh) * | 2021-06-28 | 2022-12-23 | 新泰市佳禾生物科技有限公司 | 一种将l-色氨酸转化为色胺和d-色氨酸的方法 |
-
1990
- 1990-02-21 JP JP2503856A patent/JP2919069B2/ja not_active Expired - Lifetime
- 1990-02-21 CA CA 2010582 patent/CA2010582C/fr not_active Expired - Lifetime
- 1990-02-21 WO PCT/CA1990/000057 patent/WO1990010073A1/fr not_active Ceased
- 1990-02-21 EP EP19900903729 patent/EP0425597A1/fr not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO1990010073A1 (fr) | 1990-09-07 |
| JP2919069B2 (ja) | 1999-07-12 |
| EP0425597A1 (fr) | 1991-05-08 |
| CA2010582A1 (fr) | 1990-08-24 |
| JPH03505161A (ja) | 1991-11-14 |
Similar Documents
| Publication | Publication Date | Title |
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