CA2000661C - Procede de production de plants transgeniques a valeur nutritive amelioree par l'expression d'albumines modifiees - Google Patents

Procede de production de plants transgeniques a valeur nutritive amelioree par l'expression d'albumines modifiees

Info

Publication number
CA2000661C
CA2000661C CA002000661A CA2000661A CA2000661C CA 2000661 C CA2000661 C CA 2000661C CA 002000661 A CA002000661 A CA 002000661A CA 2000661 A CA2000661 A CA 2000661A CA 2000661 C CA2000661 C CA 2000661C
Authority
CA
Canada
Prior art keywords
recombinant dna
plant
nucleic acid
albumin
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002000661A
Other languages
English (en)
Other versions
CA2000661A1 (fr
Inventor
Ann De Clercq
Enno Krebbers
Joel Vandekerckhove
Luiz Barreto De Castro
Eugen Gander
Marc Van Montagu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer CropScience NV
Empresa Brasileira de Pesquisa Agropecuaria EMBRAPA
Original Assignee
Plant Genetic Systems NV
Empresa Brasileira de Pesquisa Agropecuaria EMBRAPA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP88402650A external-priority patent/EP0318341B1/fr
Application filed by Plant Genetic Systems NV, Empresa Brasileira de Pesquisa Agropecuaria EMBRAPA filed Critical Plant Genetic Systems NV
Publication of CA2000661A1 publication Critical patent/CA2000661A1/fr
Application granted granted Critical
Publication of CA2000661C publication Critical patent/CA2000661C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253—Methionine or cysteine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA002000661A 1988-10-14 1989-10-13 Procede de production de plants transgeniques a valeur nutritive amelioree par l'expression d'albumines modifiees Expired - Fee Related CA2000661C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP88402611 1988-10-14
EP88402611.3 1988-10-14
EP88402650.1 1988-10-20
EP88402650A EP0318341B1 (fr) 1987-10-20 1988-10-20 Procédé pour la production de plantes transgéniques de valeur nutritionnelle augmentée par expression des albumines de stockage 2S modifiées dans ces plantes
CA000581160A CA1337048C (fr) 1987-10-20 1988-10-25 Procede pour la production de peptides a activite biologique par l'expression de genes modifies qui codent pour des proteines de stockage dans les graines chez des plantes transgeniques

Publications (2)

Publication Number Publication Date
CA2000661A1 CA2000661A1 (fr) 1990-04-14
CA2000661C true CA2000661C (fr) 1999-04-13

Family

ID=27168086

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002000661A Expired - Fee Related CA2000661C (fr) 1988-10-14 1989-10-13 Procede de production de plants transgeniques a valeur nutritive amelioree par l'expression d'albumines modifiees

Country Status (4)

Country Link
JP (1) JP2947843B2 (fr)
AU (1) AU634987B2 (fr)
CA (1) CA2000661C (fr)
WO (1) WO1990004032A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI20030315A0 (fi) * 2003-02-28 2003-02-28 Joseph Atabekov Menetelmiä ja konstruktioita valikoitujen aminohappojen pitoisuuksien lisäämiseksi siemenissä

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD240911A1 (de) * 1983-12-31 1986-11-19 Adw Der Ddr Zi Fuer Genetik Un Verfahren zur herstellung von ernaehrungsphysiologisch verbesserten pflanzensamenproteinen
ATE92530T1 (de) * 1986-05-29 1993-08-15 Calgene Inc Transformation und expression eines fremden gens in -i(brassica) spezies.
WO1989003887A1 (fr) * 1987-10-20 1989-05-05 Plant Genetic Systems N.V. Procede de production de peptides biologiquement actifs par l'expression de genes de proteines de graines de stockage modifies, dans des plantes transgeniques
AU626821B2 (en) * 1987-10-20 1992-08-13 Plant Genetic Systems N.V. A process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants
EP0318341B1 (fr) * 1987-10-20 1996-07-31 Plant Genetic Systems, N.V. Procédé pour la production de plantes transgéniques de valeur nutritionnelle augmentée par expression des albumines de stockage 2S modifiées dans ces plantes

Also Published As

Publication number Publication date
CA2000661A1 (fr) 1990-04-14
JP2947843B2 (ja) 1999-09-13
WO1990004032A1 (fr) 1990-04-19
AU4495189A (en) 1990-05-01
AU634987B2 (en) 1993-03-11
JPH03502644A (ja) 1991-06-20

Similar Documents

Publication Publication Date Title
US5589615A (en) Process for the production of transgenic plants with increased nutritional value via the expression of modified 2S storage albumins
US5487991A (en) Process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants
US5623067A (en) Seed-specific promoter region
EP0318341B1 (fr) Procédé pour la production de plantes transgéniques de valeur nutritionnelle augmentée par expression des albumines de stockage 2S modifiées dans ces plantes
AU678154B2 (en) Oil-body protein cis-elements as regulatory signals
Vandekerckhove et al. Enkephalins prouduced in transgenic plants using modified 2S seed storage proteins
US7365242B2 (en) Suppression of specific classes of soybean seed protein genes
AU670409B2 (en) A high sulfur seed protein gene and method for increasing the sulfur amino acid content of plants
US7297847B1 (en) Amino acid-enriched plant protein reserves, particularly lysine-enriched maize γ-zein, and plants expressing such proteins
WO1991013993A1 (fr) Expression de proteines par l'intermediaire de sequences regulatrices specifiques de semences
CA2919815C (fr) Gene capable d'augmenter la teneur en proteines dans une semence, et son procede d'utilisation
WO1993020216A1 (fr) Elements cis de proteines des corps oleiferes, servant de signaux de regulation
De Clercq et al. Expression and processing of an Arabidopsis 2S albumin in transgenic tobacco
JPH08507219A (ja) 硫黄含有アミノ酸が豊富な改良された飼料作物および改良法
EP0319353B1 (fr) Procédé pour la production de peptide biologiquement actif par l'expression de gènes de protéines de réserves modifiées dans les plantes transgèniques
CA2000661C (fr) Procede de production de plants transgeniques a valeur nutritive amelioree par l'expression d'albumines modifiees
EP0723019A1 (fr) Procédé pour la production de peptide biologiquement actif par l'expression de gènes de protéines de réserves modifiées dans les plantes transgéniques
AU626821B2 (en) A process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants
DE3855455T2 (de) Verfahren zur Herstellung transgenetischer Pflanzen mit erhöhtem Nährwert durch Expression von modifizierten 2S-Speicheralbuminen in diesen Pflanzen
CA2133417C (fr) Elements cis de proteine de substance huileuse, utilises comme signaux regulateurs

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed