CA1246475A - Streptomycetes plasmid psg5, a process for obtaining it, and its use - Google Patents
Streptomycetes plasmid psg5, a process for obtaining it, and its useInfo
- Publication number
- CA1246475A CA1246475A CA000477966A CA477966A CA1246475A CA 1246475 A CA1246475 A CA 1246475A CA 000477966 A CA000477966 A CA 000477966A CA 477966 A CA477966 A CA 477966A CA 1246475 A CA1246475 A CA 1246475A
- Authority
- CA
- Canada
- Prior art keywords
- plasmid
- psg5
- foreign dna
- streptomyces
- replicon
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000013612 plasmid Substances 0.000 title claims abstract description 92
- 238000000034 method Methods 0.000 title claims description 20
- 241001655322 Streptomycetales Species 0.000 title abstract description 17
- 241000948169 Streptomyces viridosporus Species 0.000 claims abstract description 13
- 239000013600 plasmid vector Substances 0.000 claims abstract description 9
- 244000005700 microbiome Species 0.000 claims abstract description 6
- 241000588724 Escherichia coli Species 0.000 claims description 13
- 230000009089 cytolysis Effects 0.000 claims description 11
- 241000187747 Streptomyces Species 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- 230000010076 replication Effects 0.000 claims description 7
- 108091008146 restriction endonucleases Proteins 0.000 claims description 6
- 238000002955 isolation Methods 0.000 claims description 5
- 238000005520 cutting process Methods 0.000 claims description 4
- 239000006166 lysate Substances 0.000 claims description 3
- 238000010276 construction Methods 0.000 abstract description 9
- 239000013605 shuttle vector Substances 0.000 abstract description 6
- 108020004414 DNA Proteins 0.000 description 32
- 239000012634 fragment Substances 0.000 description 23
- 108090000623 proteins and genes Proteins 0.000 description 15
- 210000004027 cell Anatomy 0.000 description 12
- 238000010367 cloning Methods 0.000 description 12
- NSFFHOGKXHRQEW-UHFFFAOYSA-N Thiostrepton B Natural products N1C(=O)C(C)NC(=O)C(=C)NC(=O)C(C)NC(=O)C(C(C)CC)NC(C(C2=N3)O)C=CC2=C(C(C)O)C=C3C(=O)OC(C)C(C=2SC=C(N=2)C2N=3)NC(=O)C(N=4)=CSC=4C(C(C)(O)C(C)O)NC(=O)C(N=4)CSC=4C(=CC)NC(=O)C(C(C)O)NC(=O)C(N=4)=CSC=4C21CCC=3C1=NC(C(=O)NC(=C)C(=O)NC(=C)C(N)=O)=CS1 NSFFHOGKXHRQEW-UHFFFAOYSA-N 0.000 description 10
- 229930188070 thiostrepton Natural products 0.000 description 10
- 229940063214 thiostrepton Drugs 0.000 description 10
- NSFFHOGKXHRQEW-AIHSUZKVSA-N thiostrepton Chemical compound C([C@]12C=3SC=C(N=3)C(=O)N[C@H](C(=O)NC(/C=3SC[C@@H](N=3)C(=O)N[C@H](C=3SC=C(N=3)C(=O)N[C@H](C=3SC=C(N=3)[C@H]1N=1)[C@@H](C)OC(=O)C3=CC(=C4C=C[C@H]([C@@H](C4=N3)O)N[C@H](C(N[C@@H](C)C(=O)NC(=C)C(=O)N[C@@H](C)C(=O)N2)=O)[C@@H](C)CC)[C@H](C)O)[C@](C)(O)[C@@H](C)O)=C\C)[C@@H](C)O)CC=1C1=NC(C(=O)NC(=C)C(=O)NC(=C)C(N)=O)=CS1 NSFFHOGKXHRQEW-AIHSUZKVSA-N 0.000 description 10
- NSFFHOGKXHRQEW-OFMUQYBVSA-N thiostrepton A Natural products CC[C@H](C)[C@@H]1N[C@@H]2C=Cc3c(cc(nc3[C@H]2O)C(=O)O[C@H](C)[C@@H]4NC(=O)c5csc(n5)[C@@H](NC(=O)[C@H]6CSC(=N6)C(=CC)NC(=O)[C@@H](NC(=O)c7csc(n7)[C@]8(CCC(=N[C@@H]8c9csc4n9)c%10nc(cs%10)C(=O)NC(=C)C(=O)NC(=C)C(=O)N)NC(=O)[C@H](C)NC(=O)C(=C)NC(=O)[C@H](C)NC1=O)[C@@H](C)O)[C@](C)(O)[C@@H](C)O)[C@H](C)O NSFFHOGKXHRQEW-OFMUQYBVSA-N 0.000 description 10
- 239000000203 mixture Substances 0.000 description 8
- 229930193140 Neomycin Natural products 0.000 description 7
- 239000000872 buffer Substances 0.000 description 7
- 229960004927 neomycin Drugs 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 6
- 241000187398 Streptomyces lividans Species 0.000 description 6
- 229960005091 chloramphenicol Drugs 0.000 description 6
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 6
- 229940088598 enzyme Drugs 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000003776 cleavage reaction Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 230000007017 scission Effects 0.000 description 5
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 101100122010 Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50) glmM gene Proteins 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 102000012410 DNA Ligases Human genes 0.000 description 3
- 108010061982 DNA Ligases Proteins 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000004098 Tetracycline Substances 0.000 description 3
- 229960001701 chloroform Drugs 0.000 description 3
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 3
- 230000002779 inactivation Effects 0.000 description 3
- 229960002180 tetracycline Drugs 0.000 description 3
- 229930101283 tetracycline Natural products 0.000 description 3
- 235000019364 tetracycline Nutrition 0.000 description 3
- 150000003522 tetracyclines Chemical class 0.000 description 3
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 102000016943 Muramidase Human genes 0.000 description 2
- 108010014251 Muramidase Proteins 0.000 description 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 238000007169 ligase reaction Methods 0.000 description 2
- 229960000274 lysozyme Drugs 0.000 description 2
- 239000004325 lysozyme Substances 0.000 description 2
- 235000010335 lysozyme Nutrition 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 2
- 101150066142 tsr gene Proteins 0.000 description 2
- ITZMJCSORYKOSI-AJNGGQMLSA-N APGPR Enterostatin Chemical compound C[C@H](N)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N1[C@H](C(=O)N[C@@H](CCCN=C(N)N)C(O)=O)CCC1 ITZMJCSORYKOSI-AJNGGQMLSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 101100215133 Aspergillus oryzae (strain ATCC 42149 / RIB 40) aclI gene Proteins 0.000 description 1
- 108091036055 CccDNA Proteins 0.000 description 1
- 108010031746 Dam methyltransferase Proteins 0.000 description 1
- 241001508687 Mustela erminea Species 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 241000971019 Streptomyces geysiriensis Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/886—Streptomyces
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEP3412093.9 | 1984-03-31 | ||
| DEP3412092.0 | 1984-03-31 | ||
| DE19843412092 DE3412092A1 (de) | 1984-03-31 | 1984-03-31 | Das streptomyceten-plasmid psg5, verfahren zu seiner gewinnung und seine verwendung |
| DE19843412093 DE3412093A1 (de) | 1984-03-31 | 1984-03-31 | Hybridplasmide mit einem streptomyceten- und einem escherichia coli-replicon |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1246475A true CA1246475A (en) | 1988-12-13 |
Family
ID=25819922
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000477966A Expired CA1246475A (en) | 1984-03-31 | 1985-03-29 | Streptomycetes plasmid psg5, a process for obtaining it, and its use |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US4880746A (en, 2012) |
| EP (1) | EP0158872B1 (en, 2012) |
| JP (1) | JP2526203B2 (en, 2012) |
| AU (1) | AU582149B2 (en, 2012) |
| CA (1) | CA1246475A (en, 2012) |
| DE (1) | DE3567676D1 (en, 2012) |
| DK (1) | DK167775B1 (en, 2012) |
| ES (1) | ES541716A0 (en, 2012) |
| GR (1) | GR850797B (en, 2012) |
| PT (1) | PT80188B (en, 2012) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3614903A1 (de) * | 1986-05-02 | 1987-11-05 | Hoechst Ag | Gentamycin-resistenzgene und ihre verwendung als marker |
| DE3809692A1 (de) * | 1988-03-23 | 1989-10-12 | Hoechst Ag | Verwendung von psg5 als temperatursensitives plasmid |
| GB2338236B (en) | 1998-06-13 | 2003-04-09 | Aea Technology Plc | Microbiological cell processing |
| JP4258874B2 (ja) * | 1999-02-10 | 2009-04-30 | チッソ株式会社 | 放線菌由来の環状プラスミドｄｎａ |
| JP5582541B2 (ja) * | 2011-11-08 | 2014-09-03 | 三井造船株式会社 | 活性汚泥処理法、活性汚泥処理剤、活性汚泥処理装置および活性汚泥処理システム |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4237224A (en) * | 1974-11-04 | 1980-12-02 | Board Of Trustees Of The Leland Stanford Jr. University | Process for producing biologically functional molecular chimeras |
| DE3117131A1 (de) * | 1981-04-30 | 1982-11-25 | Hoechst Ag, 6000 Frankfurt | "plasmid psg 2 und verfahren zu seiner gewinnung" |
| US4513086A (en) * | 1981-10-15 | 1985-04-23 | Eli Lilly And Company | Cloning vectors for use in streptomyces and related organisms |
| US4416994A (en) * | 1981-10-19 | 1983-11-22 | Eli Lilly And Company | Plasmid pEL7 and related cloning vectors for use in streptomyces and related organisms |
| DE3412093A1 (de) * | 1984-03-31 | 1985-10-10 | Hoechst Ag, 6230 Frankfurt | Hybridplasmide mit einem streptomyceten- und einem escherichia coli-replicon |
-
1985
- 1985-03-23 DE DE8585103461T patent/DE3567676D1/de not_active Expired
- 1985-03-23 EP EP85103461A patent/EP0158872B1/de not_active Expired
- 1985-03-29 AU AU40599/85A patent/AU582149B2/en not_active Ceased
- 1985-03-29 CA CA000477966A patent/CA1246475A/en not_active Expired
- 1985-03-29 GR GR850797A patent/GR850797B/el unknown
- 1985-03-29 PT PT80188A patent/PT80188B/pt not_active IP Right Cessation
- 1985-03-29 DK DK145185A patent/DK167775B1/da not_active IP Right Cessation
- 1985-03-29 JP JP60064033A patent/JP2526203B2/ja not_active Expired - Lifetime
- 1985-03-29 ES ES541716A patent/ES541716A0/es active Granted
-
1987
- 1987-10-14 US US07/110,105 patent/US4880746A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ES8603572A1 (es) | 1985-12-16 |
| DK145185D0 (da) | 1985-03-29 |
| PT80188A (de) | 1985-04-01 |
| JPS60256384A (ja) | 1985-12-18 |
| AU4059985A (en) | 1985-10-03 |
| US4880746A (en) | 1989-11-14 |
| GR850797B (en, 2012) | 1985-11-25 |
| PT80188B (pt) | 1987-09-18 |
| JP2526203B2 (ja) | 1996-08-21 |
| EP0158872A1 (de) | 1985-10-23 |
| AU582149B2 (en) | 1989-03-16 |
| DK167775B1 (da) | 1993-12-13 |
| EP0158872B1 (de) | 1989-01-18 |
| DE3567676D1 (en) | 1989-02-23 |
| DK145185A (da) | 1985-10-01 |
| ES541716A0 (es) | 1985-12-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |