CA1132365A - Cuvette array for analysers - Google Patents
Cuvette array for analysersInfo
- Publication number
- CA1132365A CA1132365A CA351,588A CA351588A CA1132365A CA 1132365 A CA1132365 A CA 1132365A CA 351588 A CA351588 A CA 351588A CA 1132365 A CA1132365 A CA 1132365A
- Authority
- CA
- Canada
- Prior art keywords
- chamber
- cell
- ring
- cells
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/07—Centrifugal type cuvettes
Abstract
ABSTRACT
A ring of cells for analytical devices operating on the centrifugal principle and photometrically measuring a sample for analysis, the system comprising a number of individual cells disposed in a circle and made of thermoplastics, each cell having three interconnected chambers, i.e. a sample chamber, a reagent chamber and a photometric measuring chamber, the major axes of the sample chamber and the reagent chamber being at right angles to the plane of rotation and the major axis of the measuring chamber coinciding with the plane of rotation.
In order to simplify and thus reduce the cost of the ring of cells, each cell at its upper edge has a laterally projecting tongue which completely or partly overlaps the adjacent cell and is secured thereto. The above ring of cells is primarily intended for use in a centrifugal analyser for clinical chemistry.
A ring of cells for analytical devices operating on the centrifugal principle and photometrically measuring a sample for analysis, the system comprising a number of individual cells disposed in a circle and made of thermoplastics, each cell having three interconnected chambers, i.e. a sample chamber, a reagent chamber and a photometric measuring chamber, the major axes of the sample chamber and the reagent chamber being at right angles to the plane of rotation and the major axis of the measuring chamber coinciding with the plane of rotation.
In order to simplify and thus reduce the cost of the ring of cells, each cell at its upper edge has a laterally projecting tongue which completely or partly overlaps the adjacent cell and is secured thereto. The above ring of cells is primarily intended for use in a centrifugal analyser for clinical chemistry.
Description
` ~3Z365 RAN 4090/ll7 The invention relates to a ring of cells for analy-iical devices operating on the centrifugal principle and photometrically measuring a sample for analysis, the system comprising a number of individual cells disposed in a circle and made of thermoplastics, each cell having three 10 interconnected chambers, i.e. a sample chamber, a reagent chamber and a photometric measuring chamber, the major axes of the sample chamber and the reagent chamber being at right angles to the plane of rotation and the major axis of the measuring chamber coinciding with the plane of rotation.
Some known analytical devices operate on the centri-fugal principle and make photometric measurements of the sample for analysis. They are preferably used when very small samples have to be analysed in a short time and where 20 the nature of the analysis is such that photometric methods can be used. This applies particularly to clinical chemical analysis, where it is frequently necessary e.g. to determine glucose, urea, uric acid, cholesterol or total protein, albumin, bilirubin or metal ions in the blood, serum, plas-26 ma, urine or liquid obtained by puncture, e.g. liquor orsimilar biological solutions. The amount of material avai-lable for investigation is frequently only a few micro-litres and the time available for analysis is often limited to a few minutes. In such cases it is advantageous to use 30 automatic analytical devices operating by the aforementioned centrifugal method.
The devices comprise a ring of cells containing a predetermined number of individual cells. Each individual 35 cell has at least three chambers, i.e. a sample chamber, a reagent chamber and a photometric measuring chamber. The sample and the reagent are poured into the appropriate cell Ve/8.4.80 chambers when the rotor is motionless, so that the two liquids initially remain separate. After the cells have been filled, the analyzer is started up, whereupon the centrifuge first rotates at high speed. As a result of centrifugal force, the sample for analysis and the rea-gent liquid are expelled from the two chambers into which they were previously poured, and are combined and raised to the photometric measuring chamber. After a predeter-mined time, the photometric scanning of the individual mea-suring chamber begins, and usually the peripheral speed ofthe centrifugal part of the analyzer is reduced. The extinc-tion recorded by the measuring part is evaluated by a computer and is usually immediately expressed as the result of the analysis.
Known centrifuaal analyzers of this kind, e.g. as described in the "Cobas-Bio" pamphlet published in 1978 by Messrs. F. Hoffmann-Laroche & Co. of Basle, comprise a number of individual cells combined to form the aforemen-tioned ring of cells by means of a separate ring. The ring has a number of radial slots in which the cells with their oblong measuring chambers are inserted and retained. The advantage of the device is that individual cells can be thrown away after use and destroyed if necessary, which is particularly important in the analysis of pathogenic liquids.
German Offenleyungsschrift 27 26 219 discloses a cell system for centrifugal analyzers having a different con-struction. According to the last citation, the cell systemcomprises two superposed discs of flexible thermoplastics, the lower disc being formed with a number of compartments and the top disc being stuck or welded to the bottom disc and, in the process, covering at least part of each com-35 partment. Cell systems of this kind form an inseparableunit which, as before, has good mechanical stability but where it is impossible to remove individual cells from the .
1~3;~3.6~
unit and destroy them separately.
An object of the invention is to simplify and thus reduce the cost of annular cell systems of the kind des-6 cribed in the "Cobas-Bio" pamphlet. The object of the simplification is to do without the retaining ring without qiving up the advantage of separating individual cells.
To this end, according to the invention, each cell at 10 its upper edge has a laterally projecting tongue which completely or partly overlaps the adjacent cell and is secured thereto. According to a particularly advantageous feature, the tongue is secured, preferably by welding, to the top edge of the next-but-one side wall of the next cell. As a result of this feature, the ring of cells has sufficient dimensional stability and strength to withstand normal handling and the stress during centrifuging. It has been found that the required strength can also be obtained if the tongue covers only the rear-part of the neighbouring 20 cell in the radial direction but leaves the front part uncovered. This is advantageous in that it facilitates the process of filling individual cells when the ring is at a standstill.
Thus the present invention provides a ring of cells for analytical devices operating on the centrifugal principle and photometrically measuring a sample for analysis. The system comprises a number of individual cells disposed side by side in a circle and made of thermoplastics, each cell having an upper edge, a sample chamber, a reagent chamber 30 and a photometric measuring chamber. These chambers are interconnected with each other, by connecting apertures, the major axes of the sample chamber and the reagent chamber being at right angles to the place of rotation ~.~
, :
, ~3Z365 and the major axis of the measuring chamber coinciding with the plane of rotation. Each cell has at its upper edge a laterally projecting tongue which completely or partly overlaps, an adjacent cell and is rigidly secured thereto.
According to another proposed feature of the inven-tion, each cell has a web above the connecting aperture between the sample chamber and reagent chamber on the one hand and the measuring chamber on the other hand.
The web can be constructed with thick portions at its ends and so that the lateral end surfaces of the web of neighbouring cells abut and are interconnected if required. The webs prevent the sample or reagent liquid from accidentally reaching the relatively sensitive com-ponents of the centrifugal analyzer. The webs also support one another and further increase the strength of the ring of cells. The webs also combine to form a convenient handle for holding ~32365 the device. In spite of the resulting considerable radial strength, which easi1y withstands stresses during centri-fuging, it is possible to break individual cells from the ring and use them as partial segments in the analy-zer when only a few samples needs to 'oe analyzed.
The invention will now be described in detail with re-ference to the accompanying drawings, in which:
Fig. l is a perspective view of an embodiment of a single cell in a ring of cells according to the invention, and Fig. 2 is a plan view of a portion of a ring of cells 15 according to the invention.
Fig. l shows an individual cell (general reference l). The cell is made up of a total of three chambers, i.e.
. a reagent chamber 2, a sample chamber 3 and a photometric ; 20 chamber 4. The major axes of the reagent chamber and the sample chamber are at right angles to the plane of rota-tion of the analyzer whereas the major axis of the mea-suring chamber 4 is in the plane of rotation. Fig. l also shows that the back wall of the reagent chamber, in centri-25 fugal direction, is oblique. The corresponding wall of the sample chamber (not shown in the drawing) is likewise ob-lique. If the ring of cells, i.e. each individual cell, is centrifuged, the liquid in the reagent chamber and the sample chamber rises along the oblique wall and the liquids 30 mix and enter the measuring chamber 4.
According to the invention, the upper edge 6 of each cell l has a laterally projecting tongue 7 which partly or completely covers and is secured to the neighbouring 35 cell in the ring. Fig. 2 shows a preferred embodiment in which tongue 7 is secured to the top edge 80f the next-but-one side wall 9 of the next cell l', preferably by welding. One result of this overlap is that the reagent and cell chambers, which are open at the top, are covered by the overlapping tongue. Another result is that the entire ring of cells has higher stability, since the top edges of the chamber walls abut the under-surface of the tongue.
The drawing also shows that in the embodiment depic-ted therein tongue 7 covers only the rear part, in the 10 centrifugal direction, of the corresponding adjacent cell.
According to another proposed feature of the invention, each cell l has a web ll above the connecting aperture l0 between the sample chamber and reagent chamber on the 15 one hand and the measuring chamber on the other hand. The web preferably has thick ends 12, which increases the sta-bility and also enables the lateral end-faces 13 of the webs to abut and be interconnected if required, likewise by welding or sticking. The webs thus form a handle which 20 simplifies manipulation of the ring of cells.
Preferably the ring of cells is made of transparent, unstabilized polymethyl methacrylate (PMMA).
Some known analytical devices operate on the centri-fugal principle and make photometric measurements of the sample for analysis. They are preferably used when very small samples have to be analysed in a short time and where 20 the nature of the analysis is such that photometric methods can be used. This applies particularly to clinical chemical analysis, where it is frequently necessary e.g. to determine glucose, urea, uric acid, cholesterol or total protein, albumin, bilirubin or metal ions in the blood, serum, plas-26 ma, urine or liquid obtained by puncture, e.g. liquor orsimilar biological solutions. The amount of material avai-lable for investigation is frequently only a few micro-litres and the time available for analysis is often limited to a few minutes. In such cases it is advantageous to use 30 automatic analytical devices operating by the aforementioned centrifugal method.
The devices comprise a ring of cells containing a predetermined number of individual cells. Each individual 35 cell has at least three chambers, i.e. a sample chamber, a reagent chamber and a photometric measuring chamber. The sample and the reagent are poured into the appropriate cell Ve/8.4.80 chambers when the rotor is motionless, so that the two liquids initially remain separate. After the cells have been filled, the analyzer is started up, whereupon the centrifuge first rotates at high speed. As a result of centrifugal force, the sample for analysis and the rea-gent liquid are expelled from the two chambers into which they were previously poured, and are combined and raised to the photometric measuring chamber. After a predeter-mined time, the photometric scanning of the individual mea-suring chamber begins, and usually the peripheral speed ofthe centrifugal part of the analyzer is reduced. The extinc-tion recorded by the measuring part is evaluated by a computer and is usually immediately expressed as the result of the analysis.
Known centrifuaal analyzers of this kind, e.g. as described in the "Cobas-Bio" pamphlet published in 1978 by Messrs. F. Hoffmann-Laroche & Co. of Basle, comprise a number of individual cells combined to form the aforemen-tioned ring of cells by means of a separate ring. The ring has a number of radial slots in which the cells with their oblong measuring chambers are inserted and retained. The advantage of the device is that individual cells can be thrown away after use and destroyed if necessary, which is particularly important in the analysis of pathogenic liquids.
German Offenleyungsschrift 27 26 219 discloses a cell system for centrifugal analyzers having a different con-struction. According to the last citation, the cell systemcomprises two superposed discs of flexible thermoplastics, the lower disc being formed with a number of compartments and the top disc being stuck or welded to the bottom disc and, in the process, covering at least part of each com-35 partment. Cell systems of this kind form an inseparableunit which, as before, has good mechanical stability but where it is impossible to remove individual cells from the .
1~3;~3.6~
unit and destroy them separately.
An object of the invention is to simplify and thus reduce the cost of annular cell systems of the kind des-6 cribed in the "Cobas-Bio" pamphlet. The object of the simplification is to do without the retaining ring without qiving up the advantage of separating individual cells.
To this end, according to the invention, each cell at 10 its upper edge has a laterally projecting tongue which completely or partly overlaps the adjacent cell and is secured thereto. According to a particularly advantageous feature, the tongue is secured, preferably by welding, to the top edge of the next-but-one side wall of the next cell. As a result of this feature, the ring of cells has sufficient dimensional stability and strength to withstand normal handling and the stress during centrifuging. It has been found that the required strength can also be obtained if the tongue covers only the rear-part of the neighbouring 20 cell in the radial direction but leaves the front part uncovered. This is advantageous in that it facilitates the process of filling individual cells when the ring is at a standstill.
Thus the present invention provides a ring of cells for analytical devices operating on the centrifugal principle and photometrically measuring a sample for analysis. The system comprises a number of individual cells disposed side by side in a circle and made of thermoplastics, each cell having an upper edge, a sample chamber, a reagent chamber 30 and a photometric measuring chamber. These chambers are interconnected with each other, by connecting apertures, the major axes of the sample chamber and the reagent chamber being at right angles to the place of rotation ~.~
, :
, ~3Z365 and the major axis of the measuring chamber coinciding with the plane of rotation. Each cell has at its upper edge a laterally projecting tongue which completely or partly overlaps, an adjacent cell and is rigidly secured thereto.
According to another proposed feature of the inven-tion, each cell has a web above the connecting aperture between the sample chamber and reagent chamber on the one hand and the measuring chamber on the other hand.
The web can be constructed with thick portions at its ends and so that the lateral end surfaces of the web of neighbouring cells abut and are interconnected if required. The webs prevent the sample or reagent liquid from accidentally reaching the relatively sensitive com-ponents of the centrifugal analyzer. The webs also support one another and further increase the strength of the ring of cells. The webs also combine to form a convenient handle for holding ~32365 the device. In spite of the resulting considerable radial strength, which easi1y withstands stresses during centri-fuging, it is possible to break individual cells from the ring and use them as partial segments in the analy-zer when only a few samples needs to 'oe analyzed.
The invention will now be described in detail with re-ference to the accompanying drawings, in which:
Fig. l is a perspective view of an embodiment of a single cell in a ring of cells according to the invention, and Fig. 2 is a plan view of a portion of a ring of cells 15 according to the invention.
Fig. l shows an individual cell (general reference l). The cell is made up of a total of three chambers, i.e.
. a reagent chamber 2, a sample chamber 3 and a photometric ; 20 chamber 4. The major axes of the reagent chamber and the sample chamber are at right angles to the plane of rota-tion of the analyzer whereas the major axis of the mea-suring chamber 4 is in the plane of rotation. Fig. l also shows that the back wall of the reagent chamber, in centri-25 fugal direction, is oblique. The corresponding wall of the sample chamber (not shown in the drawing) is likewise ob-lique. If the ring of cells, i.e. each individual cell, is centrifuged, the liquid in the reagent chamber and the sample chamber rises along the oblique wall and the liquids 30 mix and enter the measuring chamber 4.
According to the invention, the upper edge 6 of each cell l has a laterally projecting tongue 7 which partly or completely covers and is secured to the neighbouring 35 cell in the ring. Fig. 2 shows a preferred embodiment in which tongue 7 is secured to the top edge 80f the next-but-one side wall 9 of the next cell l', preferably by welding. One result of this overlap is that the reagent and cell chambers, which are open at the top, are covered by the overlapping tongue. Another result is that the entire ring of cells has higher stability, since the top edges of the chamber walls abut the under-surface of the tongue.
The drawing also shows that in the embodiment depic-ted therein tongue 7 covers only the rear part, in the 10 centrifugal direction, of the corresponding adjacent cell.
According to another proposed feature of the invention, each cell l has a web ll above the connecting aperture l0 between the sample chamber and reagent chamber on the 15 one hand and the measuring chamber on the other hand. The web preferably has thick ends 12, which increases the sta-bility and also enables the lateral end-faces 13 of the webs to abut and be interconnected if required, likewise by welding or sticking. The webs thus form a handle which 20 simplifies manipulation of the ring of cells.
Preferably the ring of cells is made of transparent, unstabilized polymethyl methacrylate (PMMA).
Claims (6)
1. A ring of cells for analytical devices operating on the centrifugal principle and photometrically measuring a sample for analysis, the system comprising a number of individ-ual cells disposed side by side in a circle and made of thermo-plastics, each cell having an upper edge, a sample chamber, a reagent chamber and a photometric measuring chamber, these chambers being interconnected with each other by connecting apertures, the major axes of the sample chamber and the reagent chamber being at right angles to the plane of rotation and the major axis of the measuring chamber coinciding with the plane of rotation, and each cell having at its upper edge a laterally projecting tongue which completely or partly overlaps an adjac-ent cell and is rigidly secured thereto.
2. A ring of cells according to claim 1, characterised in that the tongue is secured by welding, to the upper edge of the next-but-one side wall of the adjacent cell.
3. A ring of cells according to claim 1 or 2, character-ised in that the tongue covers only the back part, in centrifugal direction, of the next cell.
4. A ring of cells according to claim 1, characterised in that each cell has a web above the connecting aperture between the sample chamber and reagent chamber on the one hand and the measuring chamber on the other hand.
5. A ring of cells according to claim 4, characterised in that the web has thick portions at its ends.
6. A ring of cells according to claim 4, characterised in that the ends of the webs of neighbouring cells abut and are interconnected if required.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEG7913501.6 | 1979-05-10 | ||
| DE19797913501 DE7913501U1 (en) | 1979-05-10 | 1979-05-10 | CUVETTE WREATH FOR ANALYZER |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1132365A true CA1132365A (en) | 1982-09-28 |
Family
ID=6703866
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA351,588A Expired CA1132365A (en) | 1979-05-10 | 1980-05-09 | Cuvette array for analysers |
Country Status (12)
| Country | Link |
|---|---|
| JP (1) | JPS6111637Y2 (en) |
| AT (1) | AT384489B (en) |
| AU (1) | AU518853B2 (en) |
| BE (1) | BE883205A (en) |
| CA (1) | CA1132365A (en) |
| CH (1) | CH644451A5 (en) |
| DE (1) | DE7913501U1 (en) |
| ES (1) | ES250623Y (en) |
| FR (1) | FR2456317A3 (en) |
| GB (1) | GB2049930B (en) |
| IT (1) | IT8021728V0 (en) |
| NL (1) | NL177348C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2611906B1 (en) * | 1987-03-02 | 1990-06-08 | Isa Biolog | METHOD AND APPARATUS FOR PERFORMING MEDICAL ANALYSIS |
| US5292484A (en) * | 1992-07-16 | 1994-03-08 | Spectrum Systems, Inc. | Cuvette and cuvette cartridge for a chemical analyzer |
| EP2589949A1 (en) * | 2011-11-03 | 2013-05-08 | Qiagen GmbH | Vessel with optimized measurement window for optical analysis |
| EP3222987B1 (en) | 2014-11-21 | 2019-04-10 | Sekisui Medical Co., Ltd. | Container for specimen dilution |
| CN109765395A (en) * | 2019-01-16 | 2019-05-17 | 美德太平洋（天津）生物科技股份有限公司 | The test turntable of more test positions |
-
1979
- 1979-05-10 DE DE19797913501 patent/DE7913501U1/en not_active Expired
-
1980
- 1980-04-02 CH CH260580A patent/CH644451A5/en not_active IP Right Cessation
- 1980-05-05 AU AU58075/80A patent/AU518853B2/en not_active Ceased
- 1980-05-06 IT IT2172880U patent/IT8021728V0/en unknown
- 1980-05-08 FR FR8010272A patent/FR2456317A3/en active Granted
- 1980-05-09 JP JP6375680U patent/JPS6111637Y2/ja not_active Expired
- 1980-05-09 CA CA351,588A patent/CA1132365A/en not_active Expired
- 1980-05-09 GB GB8015390A patent/GB2049930B/en not_active Expired
- 1980-05-09 BE BE0/200538A patent/BE883205A/en not_active IP Right Cessation
- 1980-05-09 ES ES1980250623U patent/ES250623Y/en not_active Expired
- 1980-05-09 NL NL8002711A patent/NL177348C/en not_active IP Right Cessation
- 1980-05-09 AT AT249380A patent/AT384489B/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| NL8002711A (en) | 1980-11-12 |
| AU518853B2 (en) | 1981-10-22 |
| IT8021728V0 (en) | 1980-05-06 |
| DE7913501U1 (en) | 1979-10-11 |
| FR2456317A3 (en) | 1980-12-05 |
| BE883205A (en) | 1980-11-10 |
| ES250623Y (en) | 1981-01-16 |
| CH644451A5 (en) | 1984-07-31 |
| ATA249380A (en) | 1987-04-15 |
| JPS55161242U (en) | 1980-11-19 |
| NL177348C (en) | 1985-09-02 |
| AT384489B (en) | 1987-11-25 |
| NL177348B (en) | 1985-04-01 |
| GB2049930A (en) | 1980-12-31 |
| FR2456317B3 (en) | 1981-06-26 |
| JPS6111637Y2 (en) | 1986-04-12 |
| ES250623U (en) | 1980-07-16 |
| AU5807580A (en) | 1980-11-13 |
| GB2049930B (en) | 1983-06-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |