CA1087970A - Method of preparing blood smear slides - Google Patents
Method of preparing blood smear slidesInfo
- Publication number
- CA1087970A CA1087970A CA290,401A CA290401A CA1087970A CA 1087970 A CA1087970 A CA 1087970A CA 290401 A CA290401 A CA 290401A CA 1087970 A CA1087970 A CA 1087970A
- Authority
- CA
- Canada
- Prior art keywords
- blood
- diluent
- venous blood
- slide
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000004369 blood Anatomy 0.000 title claims abstract description 131
- 239000008280 blood Substances 0.000 title claims abstract description 131
- 238000000034 method Methods 0.000 title claims abstract description 53
- 239000003085 diluting agent Substances 0.000 claims abstract description 45
- 239000002356 single layer Substances 0.000 claims abstract description 8
- 230000001133 acceleration Effects 0.000 claims abstract description 7
- 210000000601 blood cell Anatomy 0.000 claims abstract description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 31
- 238000005534 hematocrit Methods 0.000 claims description 26
- 238000009987 spinning Methods 0.000 claims description 22
- 238000007865 diluting Methods 0.000 claims description 12
- 239000011780 sodium chloride Substances 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000000243 solution Substances 0.000 claims description 4
- 239000008363 phosphate buffer Substances 0.000 claims 3
- 239000007975 buffered saline Substances 0.000 claims 2
- 238000004458 analytical method Methods 0.000 abstract description 10
- 238000000926 separation method Methods 0.000 abstract description 10
- 238000002156 mixing Methods 0.000 abstract description 5
- 230000002489 hematologic effect Effects 0.000 abstract 2
- 238000000386 microscopy Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 23
- 239000000523 sample Substances 0.000 description 15
- 239000012895 dilution Substances 0.000 description 10
- 238000010790 dilution Methods 0.000 description 10
- 210000003743 erythrocyte Anatomy 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 8
- 238000003909 pattern recognition Methods 0.000 description 5
- 238000013459 approach Methods 0.000 description 4
- 210000000265 leukocyte Anatomy 0.000 description 4
- 238000009826 distribution Methods 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 238000009827 uniform distribution Methods 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 210000001772 blood platelet Anatomy 0.000 description 2
- 239000012470 diluted sample Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 241000249931 Doronicum maximum Species 0.000 description 1
- 101100286668 Mus musculus Irak1bp1 gene Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000000644 isotonic solution Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- IOVGROKTTNBUGK-SJCJKPOMSA-N ritodrine Chemical compound N([C@@H](C)[C@H](O)C=1C=CC(O)=CC=1)CCC1=CC=C(O)C=C1 IOVGROKTTNBUGK-SJCJKPOMSA-N 0.000 description 1
- 239000012898 sample dilution Substances 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
- G01N2001/2846—Cytocentrifuge method
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US74139176A | 1976-11-12 | 1976-11-12 | |
| US741,391 | 1985-06-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1087970A true CA1087970A (en) | 1980-10-21 |
Family
ID=24980553
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA290,401A Expired CA1087970A (en) | 1976-11-12 | 1977-11-08 | Method of preparing blood smear slides |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JPS5387289A (enrdf_load_stackoverflow) |
| CA (1) | CA1087970A (enrdf_load_stackoverflow) |
| DE (1) | DE2750885A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2370970A1 (enrdf_load_stackoverflow) |
| GB (1) | GB1589935A (enrdf_load_stackoverflow) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3577267A (en) * | 1968-03-19 | 1971-05-04 | Us Health Education & Welfare | Method of preparing blood smears |
-
1977
- 1977-11-08 CA CA290,401A patent/CA1087970A/en not_active Expired
- 1977-11-10 FR FR7734025A patent/FR2370970A1/fr active Granted
- 1977-11-11 JP JP13558577A patent/JPS5387289A/ja active Pending
- 1977-11-11 GB GB4710477A patent/GB1589935A/en not_active Expired
- 1977-11-14 DE DE19772750885 patent/DE2750885A1/de not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5387289A (en) | 1978-08-01 |
| DE2750885A1 (de) | 1978-05-18 |
| FR2370970A1 (fr) | 1978-06-09 |
| GB1589935A (en) | 1981-05-20 |
| FR2370970B1 (enrdf_load_stackoverflow) | 1984-03-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry | ||
| MKEX | Expiry |
Effective date: 19971021 |