CA1073382A - Immobilized proteins and method of preparing same - Google Patents
Immobilized proteins and method of preparing sameInfo
- Publication number
- CA1073382A CA1073382A CA260,069A CA260069A CA1073382A CA 1073382 A CA1073382 A CA 1073382A CA 260069 A CA260069 A CA 260069A CA 1073382 A CA1073382 A CA 1073382A
- Authority
- CA
- Canada
- Prior art keywords
- enzyme
- filler particles
- composite
- microporous
- binder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims abstract description 48
- 108010058683 Immobilized Proteins Proteins 0.000 title abstract 2
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- 239000011230 binding agent Substances 0.000 claims abstract description 38
- 239000000126 substance Substances 0.000 claims abstract description 22
- 230000003100 immobilizing effect Effects 0.000 claims abstract description 4
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- 229940088598 enzyme Drugs 0.000 claims description 138
- 238000006243 chemical reaction Methods 0.000 claims description 60
- 239000000758 substrate Substances 0.000 claims description 39
- 239000000463 material Substances 0.000 claims description 32
- 239000011148 porous material Substances 0.000 claims description 30
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 27
- 239000002131 composite material Substances 0.000 claims description 19
- 239000000543 intermediate Substances 0.000 claims description 17
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical group O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 13
- 108010015776 Glucose oxidase Proteins 0.000 claims description 12
- 239000004366 Glucose oxidase Substances 0.000 claims description 12
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- 235000019420 glucose oxidase Nutrition 0.000 claims description 12
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- WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical group CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 claims description 6
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- 239000011593 sulfur Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S530/811—Peptides or proteins is immobilized on, or in, an inorganic carrier
Landscapes
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US60907775A | 1975-08-29 | 1975-08-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1073382A true CA1073382A (en) | 1980-03-11 |
Family
ID=24439269
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA260,069A Expired CA1073382A (en) | 1975-08-29 | 1976-08-27 | Immobilized proteins and method of preparing same |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US4102746A (enExample) |
| JP (1) | JPS6029474B2 (enExample) |
| BR (1) | BR7605647A (enExample) |
| CA (1) | CA1073382A (enExample) |
| DE (1) | DE2639234C2 (enExample) |
| DK (1) | DK149312C (enExample) |
| FR (1) | FR2322156A1 (enExample) |
| GB (1) | GB1550128A (enExample) |
| IT (1) | IT1075203B (enExample) |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4251631A (en) * | 1978-02-23 | 1981-02-17 | Research Products Rehovot Ltd. | Cross-linked enzyme membrane |
| US4343901A (en) * | 1980-10-22 | 1982-08-10 | Uop Inc. | Magnetic support matrix for enzyme immobilization |
| DE3130924A1 (de) * | 1981-08-05 | 1983-02-17 | Röhm GmbH, 6100 Darmstadt | Oberflaechenreiche systeme zur fixierung von nucleophile gruppen enthaltenden substraten |
| US4557900A (en) * | 1982-09-28 | 1985-12-10 | Cardiovascular Devices, Inc. | Optical sensor with beads |
| US4474879A (en) * | 1982-11-16 | 1984-10-02 | Eli Lilly And Company | Process for 3-hydroxymethyl cephalosporin sulfones |
| US4663163A (en) * | 1983-02-14 | 1987-05-05 | Hou Kenneth C | Modified polysaccharide supports |
| WO1984003053A1 (en) * | 1983-02-14 | 1984-08-16 | Amf Inc | Modified polysaccharide supports |
| DK317483D0 (da) * | 1983-07-08 | 1983-07-08 | Superfos As | Immobiliseret enzympraeparat og fremgangsmade til fremstilling deraf |
| US4724207A (en) * | 1984-02-02 | 1988-02-09 | Cuno Incorporated | Modified siliceous chromatographic supports |
| US4687820A (en) * | 1984-08-22 | 1987-08-18 | Cuno Incorporated | Modified polypeptide supports |
| EP0191355B1 (en) | 1985-01-30 | 1989-04-19 | Kao Corporation | A fastener tape for disposable diaper |
| US4861714A (en) * | 1985-04-04 | 1989-08-29 | Verax Corporation | Weighted collagen microsponge for immobilizing bioactive material |
| US5100783A (en) * | 1985-05-10 | 1992-03-31 | Verax Corporation | Weighted microsponge for immobilizing bioactive material |
| EP0228460A1 (en) * | 1985-07-10 | 1987-07-15 | CLYDE, Robert A. | Process and apparatus for enhancing biological and chemical reactions from high area inorganic base silica on fibers |
| US4861644A (en) * | 1987-04-24 | 1989-08-29 | Ppg Industries, Inc. | Printed microporous material |
| US4833172A (en) * | 1987-04-24 | 1989-05-23 | Ppg Industries, Inc. | Stretched microporous material |
| US4927802A (en) * | 1988-12-09 | 1990-05-22 | Ppg Industries, Inc. | Pressure-sensitive multi-part record unit |
| US5019270A (en) * | 1989-07-06 | 1991-05-28 | Perseptive Biosystems, Inc. | Perfusive chromatography |
| US5228989A (en) * | 1989-07-06 | 1993-07-20 | Perseptive Biosystems, Inc. | Perfusive chromatography |
| US5047283A (en) * | 1989-09-20 | 1991-09-10 | Ppg Industries, Inc. | Electrically conductive article |
| US5155144A (en) * | 1990-10-29 | 1992-10-13 | Manganaro James L | Polysaccharide-based porous sheets |
| US5194279A (en) * | 1991-06-20 | 1993-03-16 | Ppg Industries, Inc. | Glucoamylase removal using nitrogen-functionalized amorphous precipitated silica |
| US5993935A (en) * | 1991-10-11 | 1999-11-30 | 3M Innovative Properties Company | Covalently reactive particles incorporated in a continous porous matrix |
| US5177242A (en) * | 1991-12-17 | 1993-01-05 | Fmc Corporation | Process for preparing optically active cyanohydrins with enzymes |
| US5437861A (en) * | 1993-03-16 | 1995-08-01 | Applied Immune Sciences, Inc. | Removal of selected factors from whole blood or its components; and prevention and treatment of septic shock syndrome |
| WO1994021124A1 (en) * | 1993-03-16 | 1994-09-29 | Applied Immune Sciences, Inc. | Removal of selected factors from whole blood or its components |
| NO931809L (no) * | 1993-05-19 | 1994-11-21 | Norsk Hydro As | Hemofilter |
| DE69404848T2 (de) * | 1993-08-13 | 1998-03-19 | Minnesota Mining & Mfg | Filterpatrone mit darauf angeordneten unlöslichen enzympartikeln |
| US6893816B1 (en) * | 1993-10-28 | 2005-05-17 | Houston Advanced Research Center | Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions |
| US5922531A (en) * | 1994-06-21 | 1999-07-13 | Advanced Research And Technology | Polyelectrolyte treated glass for enzyme immobilization and protein purification |
| US5532311A (en) * | 1995-02-01 | 1996-07-02 | Minnesota Mining And Manufacturing Company | Process for modifying surfaces |
| US6689265B2 (en) | 1995-10-11 | 2004-02-10 | Therasense, Inc. | Electrochemical analyte sensors using thermostable soybean peroxidase |
| US5972199A (en) * | 1995-10-11 | 1999-10-26 | E. Heller & Company | Electrochemical analyte sensors using thermostable peroxidase |
| AU1341097A (en) * | 1996-02-07 | 1997-08-28 | W.L. Gore & Associates, Inc. | Improved filled porous polymers with surface active agents and methods of making same |
| US6146771A (en) * | 1997-07-01 | 2000-11-14 | Terumo Cardiovascular Systems Corporation | Process for modifying surfaces using the reaction product of a water-insoluble polymer and a polyalkylene imine |
| US6197289B1 (en) | 1997-07-01 | 2001-03-06 | Terumo Cardiovascular Systems Corporation | Removal of biologically active agents |
| US6306665B1 (en) | 1999-10-13 | 2001-10-23 | A-Fem Medical Corporation | Covalent bonding of molecules to an activated solid phase material |
| US6939451B2 (en) * | 2000-09-19 | 2005-09-06 | Aclara Biosciences, Inc. | Microfluidic chip having integrated electrodes |
| US6808908B2 (en) * | 2001-05-30 | 2004-10-26 | Porex Technologies Corporation | Functionalized porous substrate for binding chemical and biological moieties |
| US20050061745A1 (en) * | 2002-06-26 | 2005-03-24 | Teledyne Isco, Inc. | Separation system, components of a separation system and methods of making and using them |
| US7473367B2 (en) * | 2002-06-26 | 2009-01-06 | Dionex Corporation | Monolithic column |
| US6749749B2 (en) * | 2002-06-26 | 2004-06-15 | Isco, Inc. | Separation system, components of a separation system and methods of making and using them |
| DE10344819B4 (de) * | 2003-09-26 | 2017-06-29 | Sartorius Stedim Biotech Gmbh | Adsorptionsmembranen, Verfahren zur Herstellung derselben und Vorrichtungen, welche die Adsorptionsmembranen umfassen |
| DE10344820B4 (de) * | 2003-09-26 | 2009-04-16 | Sartorius Stedim Biotech Gmbh | Adsorptionsmembranen, Verfahren zur Herstellung derselben und Verwendung der Adsorptionsmembranen in Vorrichtungen |
| US7928282B2 (en) * | 2004-04-30 | 2011-04-19 | Kimberly-Clark Worldwide, Inc. | Absorbent products with a linked enzyme treatment |
| US9540631B1 (en) | 2004-09-14 | 2017-01-10 | Peter T. Pugliese | Immobilized glucose oxidase for use in oral hygiene |
| US20070055013A1 (en) * | 2005-02-21 | 2007-03-08 | Noriho Kamiya | Substrate and method of immobilizing protein |
| EP2022852B8 (en) * | 2006-05-29 | 2017-08-23 | Kaneka Corporation | Method for production of optically active amine compound, recombinant vector, and transformant carrying the vector |
| JP2014511900A (ja) * | 2011-03-13 | 2014-05-19 | ポーラス パワー テクノロジーズ | 表面自由エネルギーに基づく充填ポリマー微細孔膜 |
| CN103797104A (zh) * | 2011-07-12 | 2014-05-14 | 诺维信公司 | 储存稳定的酶颗粒 |
| EP3539645A1 (en) | 2018-03-16 | 2019-09-18 | HurraH S.à r.l. | Functionalised mixed matrix membranes and method of production |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2772322A (en) * | 1953-08-05 | 1956-11-27 | Us Rubber Co | Microporous vinyl chloride resin and method of making same |
| GB954202A (en) | 1959-06-08 | 1964-04-02 | Electric Storage Battery Co | Microporous thermoplastic material |
| US3519538A (en) * | 1968-09-05 | 1970-07-07 | Corning Glass Works | Chemically coupled enzymes |
| US3796634A (en) * | 1970-03-19 | 1974-03-12 | Us Health Education & Welfare | Insolubilized biologically active enzymes |
| US3696061A (en) * | 1970-04-13 | 1972-10-03 | Amerace Esna Corp | Method for forming flowable powder processable into microporous object |
| US3791927A (en) * | 1971-07-14 | 1974-02-12 | American Cyanamid Co | Entrapped carrier bound enzymes |
| US3766013A (en) * | 1971-08-24 | 1973-10-16 | American Cyanamid Co | Preparation of water-insoluble carrier bound enzymes |
| US3862030A (en) * | 1972-12-13 | 1975-01-21 | Amerace Esna Corp | Microporous sub-micron filter media |
-
1976
- 1976-08-16 US US05/714,291 patent/US4102746A/en not_active Expired - Lifetime
- 1976-08-17 GB GB34237/76A patent/GB1550128A/en not_active Expired
- 1976-08-26 IT IT51030/76A patent/IT1075203B/it active
- 1976-08-27 BR BR7605647A patent/BR7605647A/pt unknown
- 1976-08-27 CA CA260,069A patent/CA1073382A/en not_active Expired
- 1976-08-27 DE DE2639234A patent/DE2639234C2/de not_active Expired
- 1976-08-27 DK DK389376A patent/DK149312C/da not_active IP Right Cessation
- 1976-08-30 JP JP51102726A patent/JPS6029474B2/ja not_active Expired
- 1976-08-30 FR FR7626168A patent/FR2322156A1/fr active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| DK149312C (da) | 1986-09-15 |
| JPS5234978A (en) | 1977-03-17 |
| DK389376A (da) | 1977-03-01 |
| DK149312B (da) | 1986-04-28 |
| US4102746A (en) | 1978-07-25 |
| JPS6029474B2 (ja) | 1985-07-10 |
| GB1550128A (en) | 1979-08-08 |
| IT1075203B (it) | 1985-04-22 |
| FR2322156A1 (fr) | 1977-03-25 |
| DE2639234A1 (de) | 1977-03-10 |
| FR2322156B1 (enExample) | 1981-12-11 |
| BR7605647A (pt) | 1977-08-09 |
| DE2639234C2 (de) | 1984-04-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |