CA1050424A - Composition and method for determination of pregnancy - Google Patents
Composition and method for determination of pregnancyInfo
- Publication number
- CA1050424A CA1050424A CA251,114A CA251114A CA1050424A CA 1050424 A CA1050424 A CA 1050424A CA 251114 A CA251114 A CA 251114A CA 1050424 A CA1050424 A CA 1050424A
- Authority
- CA
- Canada
- Prior art keywords
- coupling agent
- neutral
- composition
- globulin
- human chorionic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims description 20
- 230000035935 pregnancy Effects 0.000 title abstract description 8
- 229940084986 human chorionic gonadotropin Drugs 0.000 claims abstract description 31
- 239000002245 particle Substances 0.000 claims abstract description 28
- 102000011022 Chorionic Gonadotropin Human genes 0.000 claims abstract description 26
- 108010062540 Chorionic Gonadotropin Proteins 0.000 claims abstract description 26
- 239000007822 coupling agent Substances 0.000 claims abstract description 23
- 125000000524 functional group Chemical group 0.000 claims abstract description 20
- 230000004520 agglutination Effects 0.000 claims abstract description 14
- 239000004816 latex Substances 0.000 claims abstract description 13
- 229920000126 latex Polymers 0.000 claims abstract description 13
- DVKJHBMWWAPEIU-UHFFFAOYSA-N toluene 2,4-diisocyanate Chemical compound CC1=CC=C(N=C=O)C=C1N=C=O DVKJHBMWWAPEIU-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000004793 Polystyrene Substances 0.000 claims abstract description 9
- 230000007935 neutral effect Effects 0.000 claims abstract description 9
- 229920002223 polystyrene Polymers 0.000 claims abstract description 9
- 230000009257 reactivity Effects 0.000 claims abstract description 7
- 210000001124 body fluid Anatomy 0.000 claims abstract description 4
- 239000010839 body fluid Substances 0.000 claims abstract description 4
- 210000002966 serum Anatomy 0.000 claims description 16
- 230000008569 process Effects 0.000 claims description 13
- 238000002360 preparation method Methods 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 18
- 239000011780 sodium chloride Substances 0.000 description 16
- 239000000243 solution Substances 0.000 description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 11
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- 239000000725 suspension Substances 0.000 description 7
- 238000003756 stirring Methods 0.000 description 6
- 239000000872 buffer Substances 0.000 description 5
- 241000283973 Oryctolagus cuniculus Species 0.000 description 4
- 239000013049 sediment Substances 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 239000003381 stabilizer Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- XUGUHTGSMPZQIW-UHFFFAOYSA-N [[4-(4-diazonioiminocyclohexa-2,5-dien-1-ylidene)cyclohexa-2,5-dien-1-ylidene]hydrazinylidene]azanide Chemical compound C1=CC(N=[N+]=[N-])=CC=C1C1=CC=C(N=[N+]=[N-])C=C1 XUGUHTGSMPZQIW-UHFFFAOYSA-N 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 229910000397 disodium phosphate Inorganic materials 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000007986 glycine-NaOH buffer Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- PJPOCNJHYFUPCE-UHFFFAOYSA-N picen-1-ol Chemical compound C1=CC=CC2=C(C=CC=3C4=CC=C5C=CC=C(C=35)O)C4=CC=C21 PJPOCNJHYFUPCE-UHFFFAOYSA-N 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 241000269350 Anura Species 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 241000588832 Bordetella pertussis Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102000006771 Gonadotropins Human genes 0.000 description 1
- 108010086677 Gonadotropins Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 239000005062 Polybutadiene Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- MTAZNLWOLGHBHU-UHFFFAOYSA-N butadiene-styrene rubber Chemical compound C=CC=C.C=CC1=CC=CC=C1 MTAZNLWOLGHBHU-UHFFFAOYSA-N 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 229940015047 chorionic gonadotropin Drugs 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- LEOJDCQCOZOLTQ-UHFFFAOYSA-N dibutylcarbamothioyl n,n-dibutylcarbamodithioate Chemical compound CCCCN(CCCC)C(=S)SC(=S)N(CCCC)CCCC LEOJDCQCOZOLTQ-UHFFFAOYSA-N 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 239000002622 gonadotropin Substances 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- QYSGYZVSCZSLHT-UHFFFAOYSA-N octafluoropropane Chemical compound FC(F)(F)C(F)(F)C(F)(F)F QYSGYZVSCZSLHT-UHFFFAOYSA-N 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229940066827 pertussis vaccine Drugs 0.000 description 1
- 229920002857 polybutadiene Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/811—Test for named disease, body condition or organ function
- Y10S436/814—Pregnancy
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
- Y10S436/817—Steroids or hormones
- Y10S436/818—Human chorionic gonadotropin
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/826—Additives, e.g. buffers, diluents, preservatives
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S530/812—Peptides or proteins is immobilized on, or in, an organic carrier
- Y10S530/815—Carrier is a synthetic polymer
- Y10S530/816—Attached to the carrier via a bridging agent
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Endocrinology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Reproductive Health (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL47223A IL47223A (en) | 1975-05-02 | 1975-05-02 | Method for the determination of pregnancy and serodiagnostic composition therefor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1050424A true CA1050424A (en) | 1979-03-13 |
Family
ID=11048219
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA251,114A Expired CA1050424A (en) | 1975-05-02 | 1976-04-27 | Composition and method for determination of pregnancy |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US4088749A (en, 2012) |
| JP (1) | JPS51133416A (en, 2012) |
| AT (1) | AT351174B (en, 2012) |
| AU (1) | AU503343B2 (en, 2012) |
| BE (1) | BE841172A (en, 2012) |
| CA (1) | CA1050424A (en, 2012) |
| CH (1) | CH621876A5 (en, 2012) |
| DE (1) | DE2618449A1 (en, 2012) |
| ES (1) | ES447474A1 (en, 2012) |
| FI (1) | FI761207A7 (en, 2012) |
| FR (1) | FR2309867A1 (en, 2012) |
| GB (1) | GB1516591A (en, 2012) |
| IL (1) | IL47223A (en, 2012) |
| IT (1) | IT1061499B (en, 2012) |
| NL (1) | NL7604637A (en, 2012) |
| ZA (1) | ZA762527B (en, 2012) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1146852A (en) * | 1979-01-31 | 1983-05-24 | Koichi Kondo | Reagent for latex agglutination |
| IL63855A (en) * | 1981-09-16 | 1984-10-31 | Teva Pharma | Method and kit for detecting pregnancy |
| US4543339A (en) * | 1982-03-16 | 1985-09-24 | Oneill Christopher | Early pregnancy detection by detecting enhanced blood platelet activation |
| DE3210080A1 (de) * | 1982-03-19 | 1983-09-22 | Behringwerke Ag, 3550 Marburg | Anti-streptolysin o-latex-reagenz und verfahren zu seiner herstellung |
| JPS61247966A (ja) * | 1985-04-25 | 1986-11-05 | Daikin Ind Ltd | 抗原−抗体反応測定用試薬 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL125235C (en, 2012) * | 1965-04-15 | |||
| US3639558A (en) * | 1968-02-19 | 1972-02-01 | Louis Csizmas | Immunological reagent particles having proteinaceous materials covalently bonded thereto |
| US3882225A (en) * | 1968-12-09 | 1975-05-06 | Miles Lab | Direct agglutination immunological reagent |
| US3951748A (en) * | 1974-11-11 | 1976-04-20 | Medical Products, Inc. | Sensitized matrix for detection of disease |
-
1975
- 1975-05-02 IL IL47223A patent/IL47223A/xx unknown
-
1976
- 1976-04-26 US US05/680,579 patent/US4088749A/en not_active Expired - Lifetime
- 1976-04-27 CA CA251,114A patent/CA1050424A/en not_active Expired
- 1976-04-27 BE BE166492A patent/BE841172A/xx unknown
- 1976-04-27 DE DE19762618449 patent/DE2618449A1/de not_active Withdrawn
- 1976-04-27 GB GB17130/76A patent/GB1516591A/en not_active Expired
- 1976-04-27 FR FR7612446A patent/FR2309867A1/fr active Granted
- 1976-04-28 ZA ZA762527A patent/ZA762527B/xx unknown
- 1976-04-28 CH CH534276A patent/CH621876A5/de not_active IP Right Cessation
- 1976-04-29 NL NL7604637A patent/NL7604637A/xx not_active Application Discontinuation
- 1976-04-29 AT AT313476A patent/AT351174B/de not_active IP Right Cessation
- 1976-04-29 FI FI761207A patent/FI761207A7/fi not_active Application Discontinuation
- 1976-04-29 ES ES447474A patent/ES447474A1/es not_active Expired
- 1976-04-30 IT IT68063/76A patent/IT1061499B/it active
- 1976-04-30 JP JP51049813A patent/JPS51133416A/ja active Pending
- 1976-05-03 AU AU13566/76A patent/AU503343B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| IL47223A0 (en) | 1975-07-28 |
| BE841172A (fr) | 1976-10-27 |
| IT1061499B (it) | 1983-02-28 |
| US4088749A (en) | 1978-05-09 |
| FR2309867B1 (en, 2012) | 1981-10-30 |
| ZA762527B (en) | 1977-04-27 |
| GB1516591A (en) | 1978-07-05 |
| AU1356676A (en) | 1977-11-10 |
| AU503343B2 (en) | 1979-08-30 |
| CH621876A5 (en, 2012) | 1981-02-27 |
| ATA313476A (de) | 1978-12-15 |
| JPS51133416A (en) | 1976-11-19 |
| AT351174B (de) | 1979-07-10 |
| FI761207A7 (en, 2012) | 1976-11-03 |
| NL7604637A (nl) | 1976-11-04 |
| ES447474A1 (es) | 1977-07-01 |
| DE2618449A1 (de) | 1977-03-31 |
| IL47223A (en) | 1979-01-31 |
| FR2309867A1 (fr) | 1976-11-26 |
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