CA1049910A - Integral element for analysis of liquids - Google Patents

Abstract

Abstract of the Disclosure The present invention concerns a multilayer element for the analysis of liquids which comprises, in the form of an intergral arrangement, (1) a reagent layer including a composition comprising material that is reactive or otherwise interactive in the presence of a substance to be analyzed (analyte) to provide a diffusible, detectable species, and (2) a registration layer that is permeable to the detectable species and within which such species, e.g., a dye, can be detected. Also within the element, between the reagent layer and the registration layer, there is a radiation-blocking layer, such as an opaque reflecting layer, to enchance detection of the diffusible species within the registration layer. In operation, a detectable species formed within the reagent layer will permeate the registration layer whereby an analytical result produced within the element can be detected.

Description

BACKGROUND OF THE INVENTION
Field of the Inven~ion Chemical analysis of liquids such as water, food- -stuffs like mllk, and biological liquids is often desirable or necessary. Various elements to facilitate liquid analy-ses are known. Such elements have often included a reagent for a substance under analysis, termed analyte herein, which reagent, upon contacting a liquid sample containing the analyte, effects ~ormation of a colored material or another detectable change in response to the presence of the analyte.
Such elements include, for example, pH test strips and similar indicators wherein a paper or other highly absorbent carrier is impregnated with a material, chemically reactive or otherwise, that responds to contact with liquid contain-ing hydrogen ion or other analyte and either generates color or changes color. Depending on the selection of responsive material, the change is usually qualitative or, at best, semiquantitative. In certain fields, it is often required that analytical techniques yield rapid, quantita^
tive results. Much recent development work has attempted to provide elements useful in diagnostic chemical analysis, where testing of biological liquids including body fluids such as blood, serum, urine and the like must produce highly quantitative results, rapidly and conveniently~
Solution chemical techniques have enjoyed broad acceptance in the clinical laboratory environment, particu-larly in automated analysis. Such techniques, however, require analyzer equipment having intricate solution handling and transport capabilities. Analytical equipment of the "wet chemistry" variety, illustrated for example in U.S.
Patent Nos. 2,797,149; 3,036,893; and 3,526,48~ is often ' . ~

.
.
. .

expensive and may require skilled personnel, both for opera-tion and to maintain the high level of cleanliness that is needed to avoid sample to sample contamination.
As an alternative to solution chemistry, various multi-layer integral elements for non-solution, essentially dry chemical analysis have been proposed, The term uinte-gral", as used herein to describe analytical elements, ref-ers to elements containing two or more desirably discrete layers that are superposed, in substantially continuous intimate contact with adjacent layers in the element, and not separable without damage to the element. Although essentially dry analysis offers 5ub5tantial storage t handling and other conveniences as compared to wet chemistry, variations of the "dry" approach have enjoyed only limited success and have been used primarily for qualitative and semi-quantitative test purposes.
DESCRIPTION OF RELATED ART
A basic variety of integral analytical element is described in U.S, Patent No. 3,092,465~ Such multi-2Q layer elements use an absorbent fibrous carrier impreg-nated with one or more reagents, typically including a color former, over which is coated a semi-permeable membrane.
Upon contact with a test liquid, analyte passes through the membrane and into the fibrous carrier to generate color in an amount related to the concentration of analyte. The membrane prevents passage and absorption of certain inter-fering components, such as red blood cells, that could impair accurate reading of the color provided as a test result.
Analytical elements that rely on absorbent filter 3Q papers or other fibrous media to receive and distribute a liquid sample have not been popular, compared to wet chemical procedures, in applications such as clinical laboratory testing, presumably due to their inability to produce highly accurate, quantitative results. It is descri~ed in the literature that diagnostic elements using impregnated bibulous materials, such as fibrous filter papers, can pro-duce non-uniform test results~ In U.S. Patent ~o. 3,050,373, it is mentioned that precipitation can occur in the impreg-nating solutions, thereby impairing uniform distribution of reagent within the bibulous carrier or matrix. Also, elements using fibrous, bibulous materials are susceptible to the occurrence of a non-uniformity in test result that is termed "banding". "Banding" is exemplified by a test result occurring to a greater extent in one portion of the region of the element experiencing a test result, such as at the periphery of the region penetrated by an applied sample. It is apparently the result of extensive and extremely non-uniform migration of sample components or reagent chemicals within the bibulous material, possibly due to chromatographing, to provide high local concentra-tions of such chemicals. Gelatin and gelatin-like materials are described in U.S. Patent Nos. 3,061,523 and 3,104,209 as useful constituents of the impregnating solution, due apparently to their ability to restrain the high rate of such migration and consequently to encourage improvements in test result uniformity. However, gelatin and gelatin-like materials in the fibrous, reagent containing bibulous matrix decrease the rate of sample uptake as compared to the ; more highly absorbent gelatin-free matrix. Such decreased absorption can leave surface liquid on the element and necessitate washing the element to remove the excess prior to making a test determination. As a result, an upper limit on the amount of gelatin to be impregnated into a bibulous matrix is typically specified. Such properties - : , , . , : , ~ -.

10499~0 also characterize analytical elements using, without more, la.yers solely of gelatin or similar materials, as discussed in U.S. Patent No. 3,526,480.
Integral analytical elements adapted for automated test procedures have also been descri~ed, such as in U.S~
Paten~ Nos. 3,368, 872 and 3,526,480. Such descriptions refer to means for avoiding chromatographic effects (often called ringing, targeting, doughnuting or banding) in the element by immobilizing reagent or including a means to decrease the tendency of an applied sample to exert a washing effect on incorporated reagent, as by use of ~imple porous members over an absorbent, reagent containing material, such as fibrous filter paper. However, there i5 no suggestion in such descriptions of using within an element a means that not only takes up a liquid ~ample but also provides a uniform apparent concentration of a sample component such as analyte to 5ubstantially the entire portion of a reagent layer surface that is contacted by an applied sample. Such uniform apparency of concentration is extremely . 2~ important in obtaining test results appropriate for inter-pretation by automated readout, wh.ether densitometric, colorimetric, fluorimetric, or otherwise~ This is true even in the absence of gross non-uniformities such as those introduced by chromatrographic effects.
A means to provide somewhat uniform concentration .of analyte to the reagent areas of an element for dry analy-. sis has been by a technique that can be termed sample con-finement. Usually, as is described in U.S. Patent No~
3,368,872, a barrier is included on the element to confine 3Q. an applied sample in a predetermined region of the element's : surface, ~ith the result that e~ces.s liquid is usually pre-~- . ., :

sent on the element after sample application~ This can create inconveniences, as in the handling and cleanup of excess sample remaining on the element and, more seriously, can require extremely precise sample volume delivery when applying sample to the element.
There has been some recognition of the need to -promote or avoid, as desired, the migration of material between layers of integral analytical elements, as is discussed in U.S. Patent Nos. 2,761,813; 2,672,431; 2,672,432;

2,677,647; 2,923,669; 3,814,670 and 3,843,452.- However, this has been in the context of elements for determining the presence of micro-organiSms, and the elements described for such purposes-typically include at least one layer comprising a fibrous matrix and require non-discrete layers, the interface of which is a blend of the adjacent layers.
Until very recently, there was no suggestion in art relating to analytical elements of a layer or other means to receive sample constituents and to encourage them 2~ to distribute within that layer to achieve therein an apparent concentrational uniformity of analyte, analyte products or other substances to be provided, in such uni-form apparent concentration, to an associa~ed layer for analytical reactions or similar activity. In fact, as was apparently well recognized, the structural and chemical characteristics of bibulouS and other fibrous mate~ials used in most known analytical elements (such as absorbent cellulosic filter papers, glass fiber papers, wood, etc~) might impair such a result for reasons of physical restraint,

3~ non-uniform permeation of sample constituents or undesirable chemical binding. Additionally, the choice of fibrous mat-erials can frustrate highly accurate measurement due to severe non-uniformity in properties ~uch as structure and texture. It is known, for example, that in the preparati~.n of papers, starting fibers are often processed to form small-er constituent fibers, called tendrils, that increase the strength of the resultant paper. The term "fibrous", as used herein to describe material~ such as papers and the like, refers to materials prepared using preformed fi~ers or strands that are present in the finished material~ Exem-plary fibers used in preparing fibrous materials are de-scribed in U.S. Patent No. 3,867,258.
Non-uniformity in the detectable color response or other test result obtained when using integral analytical elements incorporating fibrous materials has been recog-nized as a problem associated with the use of such elements.
Improved devices using such materials to provide absorbent layers have sought to overcome the gross effect of such non-uniformity, but they have not succeeded in avolding the problem. As an example,, U.S. Patent No. 3,723,064 describes an analytical element that includes regions of different effective permeability to an analyte or reaction product of an analyte and produces a plurality of spaced-apart, threshold color indications as an analytical result~
Although the desirability of a smoothly continuous response i5 manifest, an element made as described in the '0~4 ; patent can only yield an approximate analytical result, the accuracy of which varies indirectly with increased spacing between thresholds. If the difference in permeability bet-ween regions were decreased, to narrow the interval between thresholds in the interest of increased response precision 3a over the intended dynamic range, the complexity of elements made in accordance with the '064 patent-would incrèase dramatically. No suggestion is made as to how one might ': : . . .

improve the uniformity and preci~ion of a continuously vary-ing test res~lt and, however o~tLmized, elements of the '064 patent would produce a discontinuous respon~e that would apparently ~ non-uniform wit~in each region of permea-bility due to non-uniformities associated with the use of filter papers and other fibrous materials.
U.S. Patent No. 3,791,933 describes a multi-compon-ent device for the assay of enzyme substrates and metabo-lites, such as in body fluids. However, such device is not an integral element. Rather, it is a clamped array adapted to receive a test sample, filter out or otherwise remove large sample constituents (such as proteins) and effect a test reaction to produce a detectable result, such as the generation of a color. Although glass fiber paper is disclosed as assisting in distributing a reaction mixture across a plastic viewing window, such material apparently merely assists the outward diffusion of liquid sample within the glass fiber layer to enlarge the region of the element exhibiting a test result and thereby render the result more easily visible. There is no suggestion of any means to form within the region of diffu~ion a concentra-tional uniformity of analyte, which, of course, is extremely important for the production of an analytical result that ; is of a uniform nature and, as such, precisely detectable.
Improved multilayer integral analytical elements are described in French Patent Application 7,323,599, filed June 28, 1973, now French Patent No~ 2,191,734. Such ele-ments can receive a liquid sample and spread the sample with-in a spreading layer of the element to obtain in the element 3~ a uniform apparent COncentratiQn of analyte, other appro-priate sample constituent or analyte product and produce in the presence of analyte an analytical result t~at, by v~rtue _g_ 104991(~
of its uniformlty, can be measured quantitatively by automa-ted devices, using techniques such as spectrophotometry, fluorimetry, etc. Element~ disclosed in French Patent No .
2,191,734, include spreading layers and reagent layers that contain a reactive or other~ise interactive material that, by virtue of its activity, promotes in the element a radio-metrically detectable change, such as a color change.
However, color formation or the providing of another analytical result often requires a seri~s of reactions ~hat can be difficult to control and may be subject to chemical or other interferences. As an example, the fluid under analysis or byproducts of the analytical determination can provide within the element constituents that interfere with detection of the test result. It is considered desirable, therefore, to have an element for dry analysis of liquids in which the materials characterizing the test result can be detected without interference from such constituents, which may provide unwanted color, fluorescence or the like.
Accordingly, although the analytical element of French Patent No. 2,191,734 represent a substantial advance in devices for the essentially dry analysis of liquids, elements of different or expanded capabilities would be desirable.
SUMMARY OF THE INYENTION
The present invention provides novel integral elements for analysis of liquids, such as biological liquids~
As referred to herein, the terms "integral element" and "integral analytical element" refer to composite elements including an "integral" array of at least two superposed layers. Elements of this invention are capable of performing internally a variety of sample handling and/or processing functions. They do not require expertise in their use and, .

especially in their preferred embodiments, they can produce quantitative analytical result~ without specialized spotting or other procedures such as sample confinement, washing or other removal of excess sample. Further, the results pro-duced by elements of this invention are substantially con-sistent and free from deleterious internal variations so that automated means of measuring electromagnetic radiation ~radiometric techniques) can be used to detect such re-sults, if necessary or desirable, with minimal risk of inconsistency.
Stated re particularly, the present invention provides integral analytical elements composed of multiple, superposed layers which can provide quic~ly within the element a detectable change in response to the presence of an analyte in liquid applied to the element~ Elements of this invention can be used for diagnostic analysis of biological li~uids, such as blood, blood serum or urine, and include, in fluid contact, (1) a reagent layer that is permeable to at least analyte or an analyte precursor and which has therein a composition containing material that is interactive in the presence of analyte to provide a detectable chemical species, such as a dye, that i5 diffusible within the element and (2) a registration layer that is permeable to the detectable species and within which the detectable species can be detected, such as by radio-metric techniques. Registration layers as described herein ; are generally prepared without the inclusion of any such Chemically reactive materials or other materials as would interfere with such detection. The various layers of the 3~ present elements can be carried on a radiation-transmissive support. As used herein, the term "radiation-transmissive"
describes supports and ot~er layers of an analytical element, :.~ .- .
. . .

that permlt effective pas~age of electromagnetic radiation used to detect an analytical result produced in the element.
Such transmissiveness i~cludes transml~sion of electro-magnetic radiation of a wavelength or wavelengths within the region between about 200 nm and 900 nm, and also of detec-table radiation as is produced by radioactivity. Radiation-transmissive layers and supports can be transparent, if desired, and this may be beneficial for measurements to be made at low levels of radiation. When the element includes a support, the registration layer is interposed between the support and the reagent layer and usually is adjacent to the support.
~ he elements of this invention can include a radia-tion-blocking layer, which is usually interposed between the reagent layer and the registration layer. The radiation-blocking layer is a layer that contains one or more opacify-ing agents and inhibits passage in or through such layer of electromagnetic radiation ! such as at the wavelength or wavelengths used for excitation and/or detection of a 2~ species within the registration layer. Further, the subject elements can include a sample spreading layer that is in contact ~ith other layers of the element, such as the re-agent layer and the registration layer. The sample spreading layer, synonymously referred to herein as a s~reading layer or a metering layer, is capable of distributing or metering within the layer substance(s) including an analyte or an analyte precursor in a liquid sample applied to the element to provide, at any given time, a uniform apparent concentra-tion of such substance at the surface of the spreading layer 3Q facing, i.e~, closer to, the reagent layer. The applied sample need not be confined to obtain such uniform concentra-tion which, although it will be uniform at any point in time, , :

.- : - . -can change over a period of time without deleterious effects.
In various preferred embodiments, the spreading layer is isotropically porous; that is, it is porous in every direction within the layer. Reference herein to isotropic porosity identifies the fact of porosity in all direction within the spreading layer. It ~ill be understood that the degree of such porosity may be variable, if necessary or desirable, for example, regarding pore size, percentage of void volume or otherwise. The term isotropic porosity (or isotropically porous) as used herein should not be confused with the terms isoporous or ionotropic, often used with reference to filter membranes to signify those mem-branes having pores that are continuous betwe~n membrane surfaces. Likewise, isotropic porosity should not be confused with the term isotropic, used in contradistinction to the term anisotropic, which signifies filter membranes having a thin "skin" along at least one surface of the membrane. See for example, Membrane Science and Technology, ~ames Flinn ed., Plenum Press, New York (1970)~ The re-agent layer is preferably of substantially uniform permea-bility to at least one substance spreadable within the spreading layer and to the diffusible detectable species provided in the reagent layer by virtue of the interaction described herein. The registration layer i5 preferably of substantially uniform permeability to the detectable species.
Uniform permeability of a layer refers to permea-bility such that, when a homogeneous liquid is provided uniformly to a surface of the layer, identical measurements of the concentration of such fluid within the layer, but 3~ made through different regions of a surface of the layer, will usually yield substantially equal results. By virtue of uniform permeability, undesirable concentration gradients ,, - , can be avoided within, f or e~ample, a reagent layer. It iQ not necessary that all pos5ihle measurement techniques produce such results. The desira~ility of a particular technique and of specific measurement parameters will depend on the physical characteristics of the layer, such as its tendency to transmit, absorb, or scatter radiation. The selection in any instance of an appropriate measurement technique ~e.g., colorimetric, densitometric, fluorimetric) and of appropriate measurement parameters (e.g~, aperture siæe and configuration) will be apparent to those familiar with analytical procedures.
As di~cussed elsewhere herein, uniform permeability is not considered characteristic of fibrous materials such as filter paper. It is believed that factors such as variable wicking action within a fibrous material, dif-ferences in fiber size or spacing and the like, can effect the formation within such fibrous materials, and also in associated materials in fluid contact therewith, of varia-tions in the a~parent concentration or permeant liquids. ~his of course, introduces undesirable bias between test measure-ments made within regions having different apparent con centrations. Uniform permeability of reagent, registra-tion or other layers within an analytical element is desirable as a means of facilitating the convenient detec-tion of analytical results. The analytical significance of results produced in elements not having uniformly permeable layers can be limited. Also, the efficiency of result detection in such elements may be impaired, for example if irregularly occurring concentrational or other 3~ discontinuities, seen by a means of detection, are present ~ithin an element. -Reference herein to fluid contact ~et~ien a spread-.., , , . ~ , . .. . .
.. , . . . .,. : ,, , . ~ : :. . . . . .

iO499~0 ing layer, a reagent layer and/or other layer~ of an integral analytical element as described herein identifies the ability of a fluid, whether liquid or gaseous, to pass in such ele-ment between superposed regions of such layers. Stated in another manner, fluid contact refers to the ability to transport components of a fluid between the layers in fluid contact. In the case of analysis for nitrogen containing compounds, ammonia or other nitrogen containing gaseous materials may comprise fluid passing between such layers~
Although layers in fluid contact can be contiguous, they may also be separated by intervening layers. However, layers in the element that physically intervene layers in mutual contact will also be in fluid contact therewith and will not prevent the passage of fluid between such lay-ers.
As used in the specification and claims herein, the term "diffusible" denotes the capability of a material to move effectively within an analytical element by diffu-sion when that material is carried in liquid present in the 2~ element, such as the solvent or dispersion medium of a liquid sample applied to the element. Similarly, the term "permeable" denotes the ability of a substance or layer to be penetrated effectively by a material carried, i.e.l distributed in as by dissolution or dispersion, in a liquid.
In operation, an exemplary analytical element of this invention can receive a liquid sample which, if analyte positive, initiates a chemical reaction or other interaction within the reagent layer to provide a diffusible, preferably radiometrically detectable species that differs from the 3~ reagent layer into the regi~tration layer where it can be detected. If necessary or de~irable, a radiation-blocking layer can be provided in the element between the reagent -~ ~ ', ' "' ' .
.

lC~4~3910 layer and the registration layer~ Also, a metering layer can be included such that an applied sample will pass through the metering layer prior to entering the reagent layer, and analyte or an analyte precursor will be distributed within the metering layer to provide a uniform apparent concentration of such material at the surface of the meter-ing layer facing the reagent layer. It is pos~ible to obtain such uniform apparent concentration over a wide range of sample volumes applied to the element. Due to fluid contact between the metering layer and the reagent layer and also to the preferred uniform permeability of the reagent layer to substance spread within the spreading layer or to products formed by virtue of the action of such substance, u~iformly metered constituents are provided from the spreading layer to the reagent layer and can pene-trate the reagent layer essentially without the occurrence therein, at any instant in time, of significant variations in the apparent concentration of such substance or products thereof. Due to the presence of an interactive (e.g., 2Q chemically reactive~ material, and a uniform apparent concentration of substance provided from the metering layer to the reagent layer, a uniform quantitative detectable change can be produced in the element. Such a change, which can be the generation or destruction of coloration or fluorescence, can be detected quantitatively by radiometric techniques and, if desired, by automatic radiometric sensing devices such as photometric or fluorimetric devices.
BRIEF DESCRIPTION OF THE DRAWINGS
In the accompanying drawings, each of Fig. l, Fig.
3~ 2, Fig. 3 and Fig. 4 is an enlarged ectional view of a pre-ferred embodiment illustrating an integral analytical element of this invention ., . . ,. , , ~ . .

DESCRIPTION OF PREFERRED EMBODIME~TS
~ _. . ..
The integral elements of this invention include a reagent layer in fluid contact with a registration layer that is preferably radiation-transmissive. Such elements can have the layers on a support, preferably radiation-transmissive, or if the layers demonstrate appropriate durability and integrity, a support is not needed~
In one preferred embodiment, an integral analyt;cal element of this invention comprises a radiation-transmlssive support having thereon, in fluid contact, non-fibrous layers including (1) a reagent layer that is permeable to at least analyte or an analyte precursor and which contains a compo-sition having material interactive in the presence of anal-yte to provide a diffusible, detectable species, (2) a radiation-blocking layer that is permeable to the detec-table species, and (3) a radiation-transmissive registration layer that is permeable to the detectable species and within which the detectable species can be detected. Optionally, the registration layer can include a mordant for the detec-2Q table species. The registration layer i5 prefera~ly inter-posed between the support and the radiation-~locking layer, with the radiation- blocking layer interposed ~et~een the registration layer and the reagent layer~ Also, the reagent layer is preferably of substantially uniform permeability to analyte (also to an analyte precursor if appropriate) and to the diffusible, detectable species. The registration layer is of such permeability as regards the detectable species. The radiation-blocking layer, although u~ually not considered disruptive of the apparent concentration of 3~ detectable species provided to the radiation-~locking layer from the reagent layer, is de~irably of uniform permeability to the detectable species. Preferred radiat~on-~locking '~ ,' ' . , .

104g910 layers include an opacifying agent such as a pigment, a polymer in appropriate form, such as a blushed polymer, or both.
In accordance with another preferred embodiment of the present invention, there is provided an integral analy-tical element with a support having thereon, in fluid con-tact, a reagent layer, a registration layer and, optionally, a radiation-blocking layer, all as described above with respect to the foregoing preferred embodiment. Additionally, however, there is included in elementsof this preferred embodiment a metering layer r preferably isotropically porous and positioned in the element such that the reagent layer is interposed between the registration layer and the metering layer. In one aspect of the various embodiments of this invention, the layers are preferably non-fibrous~
Reagent layers in the elements of this invention are desirably uniformly permeable, and optionally porous if appropriate, to substance spreadable within the metering or spreading layer and to reaction products thereof or products formed as a result of the interaction of such sub~
stance. As used herein the term permeability includes permeability arising from porosity, ability to s~ell or any other characteristic. Reagent layers can include a matrix in which the interactive material is distributed, i.e., dissolved or dispersed. The choice of a matrix material is, of course, variable and dependent on the intended use of the element. Desirable matrix materials can include hydro-philic materials including both naturally occurring sub-stances like gelatin, gelatin derivatives, hydrophilic 3~ cellulose derivatives, polysaccharides such as dextran, ~um arabic, agarose and the like, and also synthetic substances ~-such as water-soluble polyvinyl compounds like poly~vinyl , ~ .,. .. . ~ . .
.:, . . , . . ~, ..
.. . I . :

alcohol) and poly ~e'~ acrylamide polymers, etc. Organophilic materials such as cellulose esters and the like can also be useful, and the choice of materials in any instance will reflect the u~e for which a particular element is intended. To enhance permeability of the reagent layer, if not porous, it is often useful to use a matrix material that is swellable in the solvent or dispersion medium of liquid under analysis. Also, it may be necessary to select material that is compatible ~ith the application of an adjacent layer, such as by coating means, durin~
manufacture of the element. As an example, where the forma- .
tion of discrete layers i5 desired and the intended analys-sis will be of aqueous liquids, it may be appropriate to select an essentially water soluble matrix for the reagent layer and essentially organosoluble or organo dispersible ingredients for an adjacent layer, s.uch as a spreading layer. In such manner, mutual solvent action is minimized and a clearly delineated layer structure can be formed. In many cases, to facilitate the formation within the spreading layer of such apparent concentrational uniformity as is discussed herein, it may be desirable to have the reagent layer of lower permeability than is the spreading layer it-self. Relative permeability can be determined by well-known techniques.
Within the reagent layer is distributed a material that can interact in the presence of analyte, such as the analyte of choice for which the element is optimized. Op-tionally, the interactive material can also interact with a precursor or reaction product of such an analyte, if appro-priate in view of the analysis of choice, such. as in ele-ments intended to determine cholesterol, which in serum is present in e~terified for~, and triglycerides, ~hich are , ....................................... . . .

often analyzed on the basis of the glycerol component of triglycerides. The term "interactive" is meant herein ~o refer to chemlcal reactivity, catalytic activity as in the formation of an enzyme-substrate complex, or any other form of chemical or physical interaction able to produce or promote within the element, such as in the reagent layer, the formation of a diffusible species that is radiometri-cally detectable, that is, by suitable measurement of light or other electromagnetic radiation. The distribution of interactive material can be obtained by dissolving or dis-persing it in the matrix material. Although uniform dis-tributions are often preferred, they may not be necessary if the interactive material is, for example, an enzyme.
Reagents or other interactive materials soluble in the li-quid under analysis may advantageously be immobilized in the reagent layer, particularly ~hen the reagent layer is porous. The particular interactive materials that may be distributed within a reagent layer will depend on the analy-sis of choice. In the case of many analyses, enzymes such 2Q as oxidase materials like glucose oxidase or cholesterol oxidase may desirably be included as interactive materials within a reagent layer of an element intended for the analy-sis of analyte that is a substrate for such enzyme. As an example, an oxidative enzyme can be incorporated into a reagent layer together with peroxidase or a peroxidative material and a material or composition that, upon oxidation in the presence of peroxidase (or another substance having peroxidative activity) and the hydrogen peroxide formed upon interaction of an oxidase and its substrate, provides 3Q a dye or other detectable species. In the practice of this invention, the detectable species is diffusible such that it can move into the permeable registration layer. Such ~0~9910 diffusivity can be imparted to detectable species not in-herently diffusible by means known to those Rkilled in chemical synthesis, usually by the addition of chemical groups that impart the desired solubility. Where aqueous liquids are to be analyzed, solubilizing groups such as hydroxyl groups, carboxyl groups, sulfonic acid groups and the like can be useful for purposes of solu~ilization.
Materials or composition~ that contain an oxidizable material and can provide a detectable species include certain dye-providing compositions. In one aspect, dye-providing compositions can include a compound that, when oxidized, can couple with itself or with its reduced form to provide a dye. Such autocoupling compounds include a variety of hydroxylated compounds such as orthoaminophenols, 4-alkoxynaphthols, 4-amino-5-pyrazolones, cresols, pyrogallol, guaiacol, orcinol, catechol, phloroglucinol, p,p-dihydroxy-diphenyl, gallic acid, pyrocatechoic acid, salicyclic acid, etc. Compounds of this type are well Known and described in the literature, such as in The Theory of the Photogràphic 2Q Process, Mees and James Ed, (1966), especially at Chapter 17.
In another aspect, the detectable species can be provided by oxidation of a leuco dye to provide the corresponding dyestuff form. Representative leuco dyes include such compounds as leucomalachite green and ~eucophenolphthalein.
~ther leuco dyes, termed oxichromic compounds, are described in U.S. Patent No. 3,880,658 and it is further described that such compounds can be diffusi~le with appropriate substituent groups thereon. The non-stabilized oxichromic compounds described in U.S. Patent No. 3,880,658 are 3~ considered preferable in the practice of this invention. In yet another aspect, the detectable species can be provided by dye-providing compositions that include an oxidizable - . ~ ~ . : , .. . . . . .

iC~49910 compound capable of undergoing oxidative condensation with couplers such as those containing phenolic groups or acti-vated methylene groups, together with such a coupler. Re-presentative such oxidizable compounds include such compounds as benzidene and its homologs, p-phenylenediamines, p-aminophenols, 4-amino-antipyrine, etc. A wide range of such couplers, including a num~er of autocoupling com-pounds, is described in the literature, such as in Mees and James (supra) and in Kosar, Light-Sensitive Systems, 1965 pages 215-249.
Preferred dye-providing compositions include 4-methoxy-l-naphthol, an autocoupling species, and the combina-tion of 4-aminoantipyrine (HCl) as an oxidizable compound together with 1,7-dihydroxynaphthalene as a coupler.
To facilitate the detection of any change produced in an element as described herein, such as change in colora-tion, optical denæity or fluorescence, the elements of this invention include a layer to receive any reaction products or other materials produced in a reagent layer, the relative presence or absence of which relates to detection of an analytical result. Such a layer, referred to herein as a registration layer, is permeable to detectable species - formed in the element and is in fluid contact with a reagent layer. The registration layer may be separated from re-agent layer(s) by a radiation-blocking layer, such as a reflecting and/or opaque layer, to facilitate result detec-tion by various radiometric techniques. The registration layer, which is also desirably swellable in liquid under analysis, can include hydrophilic colloids such as those useful in reagent layers. The registration layer should also be radiation-transmissive to facilitate result detec-tion. Additionally, where the detectable species produced ,.. , , , . . , . , -- ~ , :

i~ the element is a dye, the registration lay~r may contain mordant material for the dye, such as those de~cri~ed as useful image dye mordants in color photographic films and papers. Exemplary mordants are materials such as vinyl-pyridine compounds of the type disclosed in u.s. Patent No. 2,484,430; polymers containing quaternary ammonium groups such as those disclosed in U.S~ Patent Nos. 3,625,694;
3,758,445; 3,709,690; 3,488,7Q6 and 3,557,006. An exemplary mordant is N,N-dimethyl-N-benzyl-3-maleimidopropylammonium chloride.
As mentioned previously, elements of this invention can include a radiation-blocking layer, preferably inter-posed bçtween a reagent layer and the registration layer.
Radiation-blocking layers are permeable to the detectable species formed in the element and serve to inhibit passage of electromagnetic radiation, such as at the wavelength or wavelengths used for detection. Using such a layer, color or other potential interferants to result detection can be ; kept from the registration layer. Such layers include an 2~ opacifying agent that, by virtue of its absorbance, reflec-tance or the like, provides a radiation inhibitin~ effect when incorporated into the layer~ In one aspect, the radia-tion-blocking layer can include a matrix containing an opacifying agent, such as a pigment like carbon or other inorganic pigment such as a metal salt like titanium dioxide, zinc oxide, barium sulfate, etc. Blushed polymers, which are generally reflective in nature, can comprise the opaci-fying agent and layers of such blushed polymers as are use-ful in spreading layers can be used also as radiation-3~ blocking layers. It will be appreciated that if a micropor-ous, blushed polymer layer is used as a radiation-blocking layer, such layer can also serve as a filtering layer.

-23_~

.
, . . .
. , . ~ ,: . .

In one preferred aspect, blushed polymer layers can also incorporate a reflective inorganic pigment, such as the highly reflective pigments mentioned elsewhere herein, to enhance spreading and/or reflectivity. The amount of pigment that can be included in a layer together with blushed polymer is highly variable, and amounts of from about 5 per cent by weight to aboutl,oo~ per cent by weight of pigment based on the weight of blushed polymer are pre-ferred, with a pigment concentration of from about 100 weight per cent to about 600 weight per cent pigment based on the blushed polymer being most preferred.
In one embodiment, the integral elements of this invention can include an isotropically porous spreading layer in fluid contact with a reagent layer~ The spreading layex is a layer, isotropically porous or otherwise, that can accept a liquid sample, whether applied directly to the spreading layer or provided to it from a layer or layers in fluid contact with the spxeading layer, and within which the solvent or dispersion medium of the sample and at least one solute, dispersoid tconstituent of the dispersed or internal phase) or reaction product of solute or dispersoid is distributed such that a uniform apparent concentration of such substance, i.e. solute, dispersoid or reaction product thereof (which can be an analyte or an analyte precursor~, is provided at the surface of the spreading layer facing the reagent layer(s) of the element. It will be appreciated that such an apparent concentration can be achieved with concentration gradients present through the thickness of or otherwise in the spreading layer~ Such - 3~ gradients do not present any difficulty to obtaining quanti-tative test results and can be accommodated using known calibration techniques.

. , .
. .,.-. . ,:
. . .
. ~ , .

The mechanism of spreading is not fully understood, but it is believed that spreading re~ults rom and is limited by a combination of forces such as hydrostatic pressure of a liquid sample, capillary action within the spreading layer, surface tension of the sample, wicking action of layers in fluid contact with the spreading layer, and the like. As will be appreciated, the extent of spreading is dependent in part on the volume of liquid to ~e spread.
Ho~ever, it should be emphasized that the uniform apparent concentration obtained with spreading is substantially in-dependent of liquid sample volume and will occur with varying degrees of spreading. As a result, elements of this inven-tion do not require precise sample application techniques.
However, a particular liquid sample volume may be desirable for reasons of preferred spread times or the like. Be-cause the elements of this invention are able to produce quantitative results using very small sample volumes that can be entirely taken up within a conveniently sized region of the spreading layer (e.g. one square centimeter), there 2Q is no need to remove excess moisture from the element after application of a liquid sample. Further, because spreading occurs in the spreading layer and the spread substance i5 :
provided to the fluid contacting reagent layer and without apparent su~stantial lateral hydrostatic pressure, there is not the "ringing'l problem often seen with prior analytical elements.
The spreading layer need only produce a uniform apparent concentration of spread substance per unit area at its surface facing a reagent layer vith which the spreading 3n layer is in fluid contact, and it is ~ery convenient to de-termine whether a particular layer can be suitable for spreading purposes. Such uniformity of apparent concentra-.. . ..
. , .. ~ ,.. . .

tion can be determined by densitometric or other analytical techniques, as by scanning the appropriate surface or re-agent layer or other associated layer to determine the apparent concentration of spread substance or of any reac-tion product based on th0 concentration of spread substance.
The following test is intended only as an example and the selection of materials or test parameters does not indicate, expressly or by implication, that other materials or para-meters would not be suitable for ~imilar purposes.
In conducting such a test one can apply to a transparent photographic film support material, such as subbed poly (ethylene terephthalate~, a transparent gelatin layer at a gelatin coverage of about 200 mg/dm2. The gelatin may vary in hardness, but for testing purposes a layer of gelatin hardened to swell the layer thickness by about 300%
when immersed for 5 minutes in 22C water is suitable. When dry, the gelatin layer will have a thickness of about 30 microns. Over the gelatin layer can be applied, such as by coating from solution or dispersion, the layer to be evaluat-ed for spreading purposes. Spreading layers can be designed to have widely varying dry thicknesses, and a thickness of from about 100 to about 200 microns, i5 convenient for test purposes. After drying the layers, a sample of test solu-tion or dispersion can be applied to the surface of the spreading layer under evaluation, preferably in a small quantity so that not all portions of the layer are wetted by the applied sample, but desirably sufficient to create a wetted region such as one having a circular area of about ; 8-10 millimeters in diameter. The selection of a test solu-3~ tion or dispersion is a matter of choice and will depend in part on the type of sample or analyte to which the layer will be exposed under conditions of actual usage. For lo~

.

10~9910 molecular weight materials, aqueous dye solutions can be used and a .0005 w~ight per cent solution of Solatine PinkR
is acceptable. For higher molecular weight materials such as proteins, an aqueous dispersion of bovine albumin dyed with Solatine Pink~ can be used. After applying the liquid sample to the layer under evaluation and allowing the liquid sample to disappear from the surface of and be taken up into the layer, the test element can be turned over and the bottom surface of the proposed spreading layer can be viewed through the transparent support material and gelatin layer, If, prior to substantial evaporation of solvent or disper-, sion medium, the test element exhibits a well-defined colored spot of a substantially uniform color density when scanned by a densitometer having an aperture of about 5 microns by 100 microns, then spreading and the achievement of a uniform apparent concentration at the bottom surface of the test layer and/or in the gelatin layer has taken place. By substantially uniform density is meant a density across the spot, with the exception of its periphery, having 2~ maximum and minimum values not more than about + 10 from the mean density. Due to edge effects,,,non-characteristic density gradients may arise at the spot periphery but need have no effect on the significance of an analytical result.
Peripheral area can vary between spots, but it will usually not be more than about 20% of the entire spot and may be less.
As mentioned herein, useful spreading or metering layers can be isotropically porous layers. Such layers can be prepared using a variety of components~ In one aspect, 3a particulate material can be used to form such layers, wherein the isotropic porosity i5 created by interconnected spaces between the particles. Various types of particulate .
.~ . .. . . . . . .

matter, all desirably chemically inert to sample components under analysis, are useful. Pigments, such as titanium dioxide, barium sulfate, zinc oxide, lead oxide, etc., are desirable. Other desirable particles are diatomaceous earth and microcrystalline colloidal materials derived from natural or synthetic polymers. Such microcrystalline materials are described in an article entitled, "Colloidal Macromolecular Phenomena, Part II, Novel Microcrystals of Polymers" by O.A. Battista et al published in the $ournal of Applied Polymer Science, Vol. II, pages 481-498 ~1967).
Microcrystalline cellulose, which is commercially available from FMC Corporation under the name AvicelR, is an example of such a colloidal material ~hich i5 satisfactory for use in the present invention. Spherical particles of uniform size or sizes, such as resinous or glass beads, can also be used and may be particularly desirable where uniform pores are advantageous, such as for selective filtration pur-poses. If a particulate material of choice is not adherent, as in the case of glass beads or the like, it can be treated 2a to obtain particles that can adhere to each other at points of contact and thereby facilitate formation of an isotropi-cally porous layer. As an example of suitable treatment, non-adherent particles can be coated with a thin adherent layer, such as a solution of hydrophilic colloid like gelatin or polyvinyl alcohol, and brought into mutual contact in a layer. When the colloid coating dries, the layer integrity is maintained and open spaces remain between its component particles.
As an alternative or in addition to such particulate 3Q materials, the spreading layer can be prepared using iso-tropically porou~ polymer~. It is possible to prepare such polymers using techniques useful in forming blus~ed polymers~

- -1~49910 Blushed polymer layers can be formed on a substrate by dissolving a polymer in a mixture of two liquids, one of which is a lower boiling, good solvent for the polymer and the other of which is of a higher boiling point and is a non-solvent or at least a poor solvent for the polymer Such a polymer solution is then coated on the substrate, and dried under controlled condition~ The lower boiling solvent evaporates more readily and the coating can become enriched in the liquid which is a poor solvent or non-solvent. As evaporation proceeds, under proper conditions,the polymer forms as an isotropically porous layer. Many different polymers can be used, singl~ or in combination, for preparing isotropically porous blushed polymer spreading layers for use in this invention, typical examples being polycarbonates, polyamides, polyurethanes and cellulose esters such as cellulose acetate.
In preparing integral analytical elements of this invention, the layers can be preformed separately and laminated to form the overall element. Layers prepared in 2~ such a manner are typically coated from solution or disper-sion on a surface from which the dried layer can be physi-cally stripped. However, a convenient method which can avoid problems of multiple stripping and lamination steps i5 to coat an initial layer on a stripping surface or a support, as desired, and thereafter to coat successive layers directly or on those coated previously. Such coating can be accomplished by hand, using a blade coating device or by machine, using techniques such as dip or bead coating.
If machine coating techniques are used, it is often possible 3a to coat adjacent layers simultaneously, using hopper coating techniques well-known in the preparation of light-sensitive photographic films and papers. If it is essential or desir-~29-.. . . . . .

1~49910 able that adjacent layers be discrete, and maintenance of layer separation by adjustment of coating formulation specific gravity is not satisfactory, as possibly in the case of porous spreading layers, the appropriate selection of components for each layer, including solvent or dispersion medium, can minimize or eliminate interlayer component migration and solvent effects, thereby promoting the forma-tion of well-defined, discrete layers. Any interlayer ad-hesion problems can be overcome without harmful effect by iO means of surface treatments including extremely thin appli~
cation of subbing materials such as are used in photographic films.
For reagent layers, a coating solution or dispersion including the matrix and incorporated interactive materials can be prepared, coated as discussed herein and dried to form a dimensionally stable layer. The thickness of any reagent layer and its degree of permeability are widely variable and dependon actual usage. Dry thicknesses of fxom about lQ
microns to about 100 microns have been convenient, although more widely varying thicknesses may be preferable in certain circumstances. For example, if comparatively large amounts of interactive material, e.g. polymeric materials like enzymes, are required, it may be desirable to use slightly thicker reagent layers.
Radiation-blocking layers and registration layers can be prepared using methods and thicknesses as used when preparing reagent layers, but with constituents appropriate for the particular layer. In the case or registration layers, in addition to their permeability and radiation-transmissive-30- ness, they are desirably subætantially free from any charac-teristic that might appear aæ or contrihute to mottle or other noise in the detection of an analytical result pro-- . . .

- . . :

duced in an integral element of the invention. For example, any variations in color or in texture w~thin the registra-tion layer, as could occur if fibrous materials, e~g~ some papers, are used as a permeable medium, may be disadvan-tageous due to non-uniform reflectance or transmittance of detecting energy. Further, although fibrous materials like filter and other papers are generally permeable overall, some such materials typically can exhibit widely ranging degrees of permeability and may not exhibit uniform per-meability, for example, based on structural variations such as fiber dimensions and spacing. As a result, such materials are not preferred in registration layers and other layers of elements of the present invention.
Spreading layers can also be prepared by coating from solution or dispersion. As stated previously, spreading and associated layers of an element are in a superposed relationship such that a spreading layer is in fluid contact with a reagent layer. The range of materials useful for inclusion in any spreading layer is widely variable as discussed herein and will u5ually include predominantly materials that are resistant to, i.e. substantially in-soluble in and non-swellable upon contact with, the liquid under analysis. Swelling of about 10-40% of the layer 15 dry thickness may be normal. The thicknless of the spreading layer is variable and will depend in part on the intended sample volume, which for convenience and cleanliness the spreading layer should be able to absorb, and on the layer's void volume, which also affects the amount of sample that can be absorbed into the layer. Spreading layers of from a~out 50 microns to about 30~ microns have been particularly useful. ~owever, wider variations in thickness are accept-able and may be desirable for particular elements~

- ~, ' ' . - `. ::
':

1049~
When preparing an isotropically porous spreading layer, it is useful to have void volume comprise at least about 25~ of the total layer volume, and void volumes of from 50-95% may be desirable. Variations in void volume of porous spreading layers can be used advantageously to modify element characteristics such as total permea~ility of the spreading layer or the time needed for s~mple spreading to occur. As can be appreciated, void volume within the layer can be controlled, for example, by selecting particulate materials of appropriate:size; or by varying the solvents or drying conditions when isotropically porous blushed polymers are used in the spreading layer. The void volume of any such layer can be calculated with reason-able accuracy by a variety of techniques such. as the sta-tistical method described in Chalkley, Journal of the Nation-: al Cancer Institute, 4, 47 (1943) and by direct weighing and determining the ratio of actual weight of the layer to the weight of solid material equaL in volume to that of the layer comparably composed of constituents from the layer~. It will be appreciated that the pore size in any case should be sufficient to permit spreading of initial sample compone~ts or other su~stances desirably provided to a reagent layer.
As mentioned previously herein,. the integral anal-ytical elements can be self-supporting or coated on a support~
Useful support materi ls include a variety of polymeric materials such as cellulose acetate, poly(ethylene tere-phthalate), polycarbonates and polyvinyl compounds such as polystyrenes, etc. A support of choice for any particular - element will be compatible with the intended mode of result 3a. detection. Preferred supports include.radiation-transmissive support materials that transmit electromagnetic radiation of a waveIength or wavelengths with~n the region between -~32-. ::

about 200 nm and about 900 nm a~ well as radiation due to radioactivity. For fluorimetric detection of analytical results through the support, it is desirable for the support to transmit over a somewhat wider band that is necessary for non-fluorescence measurements or, alternatively, to transmit at the absorption and emission spectra of the fluorescent materials used for detection. It may also be desirable to have a support that transmits one or more narrow wavelength bands and is opaque to ad;acent wave-length bands~ This could be accomplished, for example, byimpregnating or coating the support with one or more colorants having suitable absorption characteristics- ~hen an element includes a support, the reagent layer, the radiation-blocking layer (if present) and the registration layer will usually be interposed in the element between the support and the spreading layer (if present), which often is the outermost layer in an element.
The components of any particular layer of an integral analytical element of this invention, and the layer configuration of choice, will depend on the use for which an element is intended. As stated previously, spreading layer pore size can be chosen so that the layer can filter out undesirable sample components that would, for example, interfere with an analytical reaction or with the detection of any test result produced within the element. For analysis of whole blood, porous layers having a pore size of from 1 to about 5 microns are particularly useful in screening out blood cells, which-typically have a size of from about 7 to about 30 microns. If desirable, an element 3Q can include a plurality of spreading layers, each of which may be different in its ability to spread and filter. Also, if a restraint on transport of suhstances ~ithin the element ; .
,- .~ ' , , .

~049910 additional to that provided by spreading layers is needed, a filter or dialysis layer can be included at an appropriate location in the element. As an example, in analyzing for blood glucose, a dialysis layer such as a semipermeable cellulose membrane can prevent passage of proteins or other potentially interfering substances to the reagent layer.
In the layers of the element, it can be advantageous to incorporate one or more surfactant materials such as anionic and nonionic ~urfactant materials. They can, for example, enhance coatability of layer formulations and enhance the extent and rate of spreading in spreading layeræ
that are not easily wetted by liquid samples in the absence of an aid such as a surfactant. Interactive materials can also be present in the spreading layer if desirable for a particular analysis. As an example, proteins or other higher molecular weight materials can, for convenience, be divided into more easily spreadable, lower molecular weight components that may also be more suitable for an analytical reaction, such as ~y having in the spreading layer an appropriate interactive material such as an enzyme, e.g., a protease or esterase. In layers of the element it can also be desirable to include materials that can render non-active in the analy5is of choice, by chemical reaction or otherwise, materials potentially deleterious to such analysis. As an example, ascorbate oxidase may be incorporated in an element to remove ascorbate ion ~hich may interfere with analysis for glucose.
In still another aspect, an analysis of choice may require a multi-stage reaction that can best be accomplished in an element having a plurality of reagent layers, each of which may be adapted to enhance or effect particular reaction ~ 0~9910 stages. AS an example, in the determination of the enzyme known as serum glutamic-oxalacetic transaminase, sequential reactions can be used. This enzyme catalyzes the conversion at a pH of about 7.4 of ~-ketoglutarate and aspartate ions to the corresponding oxalacetate and glutamate. The oxalace-tate can be measured via coupling with the diazonium salt of the dye known as "Fast Ponceau L". To facilitate the first equilibrium that should be established before coupling, it is desirable to separate the reagents and to incorporate each of them into a separate layer ~o provide a uitable time interval for the first equilibrium to be established without hindering the establishment of this first equilibrium by the premature starting of the second reaction. Thus the glutamic acid can be incorporated in a first reagent layer which is coated over a second reagent layer that contains the salt of the dye "Fast Ponceau L".
Integral analytical elements of the present invention can be adapted for use in carrying out a wide variety of chem-ical analyses, not only in the field of clinical chemistry but in chemical research and in chemical process control laboratories. They are well suited for use in clinical test-ing of body fluids, such as blood, blood serum and urine, since in this work a large number of repetitive tests are frequently conducted and test results are often needed a very short time after the sample is taken. In the field of blood analysis, for example, the multi-layer element can be adapted for use in carrying out quantitative analyses for many of the blood components which are routinely measured.
Thus, for example, the element may be readily adapted for use in the analysis of such blood components as albumin, . . . ........... . ...... . . .
, .: . , . : . ...

104g~0 bilirubin, urea nitrogen, serum glutamic-oxalacetic trans-aminase, chloride, glucose, uric acid, and alkaline phospha-tase, as well as many other components, by appropriate choice of test reagents or other interactive materials. In analyzing blood with the analytical elements of this inven-tion, the blood cells may first be separated from the serum, by such means a~ centrifuging, and the serum applied to the element. However, it is not necessary to make such separa-tion, especially if reflective spectrophotometric analysis techniques are used to quantify or otherwise analyze the reaction product formed in the element as whole blood can be applied directly to the element and the blood cells filtexed out through the action of a filtering layer. The presence of these cells on the element will not interfere with spectro-photometric analysis if it is carried out by reflection techniques, with light being transmitted through the support and registration layer and reflected from the radiation-blocking layer or other reflecting layer such that detecting radiation does not intercept the cells. A particularly sig-nificant advantage of the integral analytical elements de-scribed herein is their ability to be used to analyze either serum or whole blood.
As can be appreciated, a variety of different elements, depending on the analysis of choice, can be prepared in accordance with the present invention. Elements can be con-figured in a variety of forms, including elongated tapes of any desired width, sheets or smaller chips. Particular elements can be adapted for one or more tests of a single type or a variety of tests of different types. In such lat-ter event, it can be desirable to coat a common support 10499~0 with one or more strips or channels, each optionally of a different composition to form a composite element suited for conducting a variety of desired tests.
Exemplary elements of this invention include those illustrated in the accompanying drawings. In Fig. 1 is represented an analytical element composed of a radiation-transmissive support 10, on which is carried a registration layer 12, a radiation-blocking layer 14 which can filter as well as provide a white background for analytical result de-tection such as by reflection spectrophotometry, and a re-agent layer 16. Detection can be done through the support, which is suitably transmissive at the detecting wavelength.
The registration layer 12 can be a hydrophilic colloid such as gelatin. Reagent layer 16 can be composed of a solution or dispersion of one or more test reagents in a binder such as gelatin, while layer 14 can be a blushëd polymer having isotropic porosity and/or such pore size as may be needed for any filtering function it is intended to perform. The layers are in fluid contact. In an alternative em~odiment of the invention shown in Fig. 2, the analytical element is com-posed of a radiation-transmissive support 20 bearing a regis-tration layer 22 in fluid contact with a reagent layer 24 and a spreading layer 27 which can also serve the function of filtering and also may provide a suitably reflective back-ground for reflection spectrophotometric detection through ;
support 20. Alternatively, layer 26 may be such that it does not reflect and detection can be accomplished in the trans-mission mode. Layer 26 can be, for example, an isotropically porous blushed polymer layer which has been coated or laminated `
j 30 over layer 24. Fig. 3 illustrates a further embodiment of _37_ ~ ' : ~ . . . .
. - . ~ :

the invention in which the analytical element is composed of support 30, registration layer 32, a radiation-blocking layer 34 which can be formed from a dispersion of a pigment like titanium dioxide in a hydrophilic colloid such as gela-tin, a reagent layer 36, and a spreading layer 38 such as an isotropically porous blushed polymer layer which can serve the functions of spreading and filtering. The various layers are in fluid contact.
A still further embodiment of the invention is shown in Fig. 4 in which the analytical element is composed of a support 40, a registration layer 42, a radiation-block-ing/filtering layer 44, reagent layer (A) 46, a reagent layer (B) 48, and a spreading/filtering layer 50. Layer 44 can be composed, for example, of titanium dioxide in blushed cellu-lose acetate and layer 50 can be composed of diatomaceous earth in blushed cellulose acetate or of glass beads mutually adhered with a hydrophilic colloid like gelatin.
The present elements are placed in use by applying to the element a sample of li~uid under analysis. Typically, an element will be formed such that an applied sample will contact a spreading layer prior to a non-spreading reagent layer and will first contact such spreading layer at its surface farther from such reagent layer. Because analytical accuracy of the present elements is not substantially diminished by variations in the volume of applied samples, especially when a spreading layer is present in the element, sample application by hand or maching is acceptable. For reasons of convenience in de-tecting an analytical result, however, reasonable consistency in sample volume may be desirable. As discussed previously, the spreading layer is also extremely desirable in minimizing the occurrence of ringing when soluble interactive materials . .
.:

~49glO
are used in a reagent layer.
In a typical analytical procedure using the present elements, which could be manual or automated, the element is taken from a supply roll, chip packet or other source and positioned to receive a free drop, contact spot or other form of liquid sample, such as from an appropriate dispenser.
After sample application, and desirably after the liquid sample has been taken up by a spreading layer, if present, the element is exposed to any conditioning, such as heating, humidification or the like, that may be desirable to quicken or otherwise facilitate obtaining any test result. If an automated procedure is used, it can also be desirable to have any spreading layer accomplish its function within several seconds, but allowing sufficient time to provide metering, which is contrasted with the almost instantaneous, unregu-lated diffusion as can be obtained with absorbent fibrous papers. This can be accomplished conveniently by appropriate selection of various parameters, such as layer thickness, void volume in porous layers, etc.
After the analytical result is obtained as a detect-able change, it is measured, usually by passing the element through a zone in which suitable apparatus for reflection, transmission or fluorescence spectrophotometry is provided.
Such apparatus would serve to direct a beam of energy, such - as light, through the support and the rPgistration layer.
The light would then be reflected, such as from a radiation-blocking layer in the element, back to a detecting means or would pass through the element to a detector, in the case of transmission detection. In a preferred mode, the analytical result is detected in a region of the element totally within -39~

. . .

104991(~
the region in which such result is produced. use of re-flection spectrophotometry can be advantageous in some sit-uations as it can effectively avoid interference from resi-dues, such as blood cells, which may have be~n left on or in the layers of the element. Conventional techniques of fluorescence spectrophotometry can also be employed if the detectable species is a fluorescent material. Detection would be accomplished using energy that excites the fluores-cent species and a detector that senses its fluorescent emission. Furthermore, when blood serum is tested or means are provided for eliminating unwanted whole blood residues, transmission techniques can be used to detect and quantify the indicating reaction products by directing a flow of radiant energy, for example, U.V. visible or I.R. radiation at one surface of the element and measuring the output of that energy from the opposing surface of the element. Gener-ally, electromægnetic radiation in the range of from about 200 to about 900 nm has been found useful for such measurements, although any radiation to which the element is permeable and which is capable of quantifying the product produced in the reagent layex can be used. Various calibration techniques can be used to provide a control for the analysis. As one example, a sample of analyte standard solution can be applied adjacent to the area where the drop of sample is placed in order to permit the use of differential measurements ln the analysis.
The following example of integral analytial elements are provided to further illustra~e the present invention.

Example 1 On a thick (180 microns) support of poly(ethylene : :

104g~10 terephthalate), having a gelatin sub, are successively applied:
1) a registration (receiving) layer containing, per ~quare meter, 2.15 g. of gelatin, 2.15 g of a mordant (copolymer of styrene and N ,N-dimathyl-N-3-maleimidopropylammonium chloride);
2) a porous, reflective radiation-blocking layer con-taining, per square meter, 151 g of gelatin and 11.4 g of titanium dioxide;
'' 3) a reagent (analytical) layer containing, per square meter, 17.5 g of gelatin, 1.5 g of 1-naphthol-2-sulfonic acid potassium salt, 0.73 g of disodium phosphate buffer, 0.45 g of monopotassium phosphate buffer, 0.38 g of 4-aminoantipyrine (CHl) 1.6 g of glycerine as a plasticizer, 0.09 g of peroxydase (14014 U/m2) and 0.374 g of glu- ;
cose oxidase (40440 U/m2);

4) a spreading layer containing, per square meter, 97 g of cellulose acetate and 65.5 g of titanium dioxide.
The thusly prepared element is used for the analysis of glu-cose solutions, the concentrations of which are varied from 0 to 800 mg per deciliter. On samples of the element are deposited drops, each of which represents 10 ~1 of an aqueous glucose solution. After one hour, the density of the color-ations is measured by reflection using a Mac~eth Densitometer - (Model TD-504). When the glucose solution is applied to the surface of the element, it spreads within a layer (4) and is metered to layer ~3) wherein the glucose reacts with the oxy-gen and the water in the presence of the glucose oxidase to provide gluconic acid and hydrogen peroxide; these c~mpounds, ~41-:' ~; : .~; , ...................... .
.: .- ~ .
~' ~ -. , 1049~10 in the presence of peroxidase, react with the 4-aminoanti-pyrine which is then oxidized; the oxidation product of the 4-aminoantipyrine then reacts by coupling with the 1-naphthol-2-sulfonic acid potassium salt to form a dye which diffuses out of the reagent layer (3), through the radiation-blocking layer (2) and into the registration layer (1) where it is detected using the Macbeth TD-504 densitometer. The results are summarized in the table following Example 2.

Example 2 An analytical element, having a structure identical to that of Example 1 is prepared, except that the registration layer (1) contains gelatin as the only component, at a coating weight of 4.30 g per square meter. The product is used according to the procedures described in Example 1, and the results obtained are summarized in the table hereunder.

T~BLE

Glucose content Density measured of sample by white-light Element(ma/deciliter~ reflection Example 1 0 0.28 100 0.36 150 0.37 200 0.40 300 0.47 400 0.52 600 0.53 800 0.53 ______________________________________________ _____________ Example 2 0 0.23 100 0.36 150 0.42 200 0.47 300 0.54 400 0.55 60C 0.56 800 0.60 The invention has been described in detail with ~ . . .
. " . .

.;.. .

10499~0 particular reference to certain preferred embodiments thereof, but it will be understood that variatlons and modifications can be effected within the spirit and scope of the invention.

: -43-.

Claims (5)

THE EMBODIMENTS OF THE INVENTION IN WHICH AN EXCLUSIVE PROPERTY
OR PRIVILEGE IS CLAIMED ARE DEFINED AS FOLLOWS:

1. An integral element for analysis of liquids, the element comprising a radiation-transmissive support having thereon, in fluid contact, non-fibrous layers comprising a reagent layer permeable to an analyte or an analyte precursor and comprising a composition comprising material interactive in the presence of analyte to provide a diffusible, detectable species, a radiation-blocking layer, permeable to the detectable species, and a radiation-transmissive registration layer, permeable to the detectable species and within which said species can be detected, wherein the registration layer is interposed between the support and the radiation-blocking layer and the radiation-blocking layer is interposed between the registration layer and the reagent layer.

2. An integral analytical element as described in claim 1 wherein the radiation-blocking layer comprises an opacifying agent.

3. An integral analytical element as described in claim 2 wherein the reagent layer and the registration layer are water-swellable and wherein the opacifying agent in the radiation-blocking layer comprises a pigment.

4. An integral element as described in claim 3 wherein the reagent layer comprises a hydrophilic colloid having the interactive material distributed therein, the radiation blocking layer comprises a hydrophilic colloid having a pigment distributed therein and the registration layer comprises a hydrophilic colloid.

5. An integral element as described in Claim 4 wherein the registration layer further comprises a mordant for the detectable species.