BR9911891A - Proteases fused to subtilisin inhibitor variants of streptomyces - Google Patents
Proteases fused to subtilisin inhibitor variants of streptomycesInfo
- Publication number
- BR9911891A BR9911891A BR9911891-2A BR9911891A BR9911891A BR 9911891 A BR9911891 A BR 9911891A BR 9911891 A BR9911891 A BR 9911891A BR 9911891 A BR9911891 A BR 9911891A
- Authority
- BR
- Brazil
- Prior art keywords
- ssi
- fusion proteins
- proteases
- amino acid
- acid sequence
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4703—Inhibitors; Suppressors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Abstract
Patente de Invenção: <B>"PROTEASES FUNDIDAS COM VARIANTES DE INIBIDOR DE SUBTILISINA DE STREPTOMYCES"<D>. A presente invenção relaciona-se às proteínas de fusão, em que a proteína de fusão compreende uma parte de protease; e uma parte de variante , em que a parte de variante tem uma seq³ência de aminoácidos modificada de uma seq³ência de aminoácidos de origem, em que a seq³ência de amionácidos modificada compreende uma substituição de aminoácido na posição 63 correspondendo ao SSI, e em que a seq³ência de aminoácidos de origem é selecionada do grupo consistindo em SSI, inibidores semelhantes ao SSI, e variantes de inibidores semelhantes ao SSI. Tais proteínas de fusão são úteis em composições de limpeza e em composições para o cuidado pessoal. A presente invenção também relaciona-se às composições de limpeza e às composições para o cuidado pessoal compreendendo as presentes proteínas de fusão, assim como ao DNA codificando as proteínas de fusão.Invention Patent: <B> "PROTEASES MELTED WITH STREPTOMYCES SUBTILISIN INHIBITOR VARIABLES" <D>. The present invention relates to fusion proteins, wherein the fusion protein comprises a protease part; and a variant part, where the variant part has a modified amino acid sequence from a source amino acid sequence, where the modified amion acid sequence comprises an amino acid substitution at position 63 corresponding to the SSI, and where the sequence of origin amino acids is selected from the group consisting of SSI, SSI-like inhibitors, and SSI-like inhibitor variants. Such fusion proteins are useful in cleaning compositions and compositions for personal care. The present invention also relates to cleaning compositions and personal care compositions comprising the present fusion proteins, as well as to the DNA encoding the fusion proteins.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US9190498P | 1998-07-07 | 1998-07-07 | |
PCT/US1999/015247 WO2000001831A2 (en) | 1998-07-07 | 1999-07-07 | Proteases fused with variants of streptomyces subtilisin inhibitor |
Publications (1)
Publication Number | Publication Date |
---|---|
BR9911891A true BR9911891A (en) | 2001-03-27 |
Family
ID=22230236
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR9911891-2A BR9911891A (en) | 1998-07-07 | 1999-07-07 | Proteases fused to subtilisin inhibitor variants of streptomyces |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP1093522A2 (en) |
JP (1) | JP2004500008A (en) |
CN (1) | CN1317052A (en) |
AR (1) | AR020105A1 (en) |
AU (1) | AU4862499A (en) |
BR (1) | BR9911891A (en) |
CA (1) | CA2332177A1 (en) |
WO (1) | WO2000001831A2 (en) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR0112778A (en) | 2000-07-28 | 2003-07-01 | Henkel Kommanditgellschaft Auf | Amylolytically Bacillus sp. 7-7 (dsm 12368) as well as detergent and cleaning agent with this amylolytically enzyme |
JP4557108B2 (en) * | 2000-08-11 | 2010-10-06 | ライオン株式会社 | Chimeric enzyme and detergent composition |
WO2002016619A1 (en) * | 2000-08-21 | 2002-02-28 | Novozymes A/S | Method for production of a protease-inhibitor complex |
EP1337648B1 (en) | 2000-11-28 | 2007-09-19 | Henkel Kommanditgesellschaft auf Aktien | Novel cyclodextrin glucanotransferase (cgtase), obtained from i bacillus agaradherens /i (dsm 9948) and detergents and cleaning agents containing said novel cyclodextrin glucanotransferase |
DE10162728A1 (en) | 2001-12-20 | 2003-07-10 | Henkel Kgaa | New alkaline protease from Bacillus gibsonii (DSM 14393) and washing and cleaning agents containing this new alkaline protease |
DE10163884A1 (en) | 2001-12-22 | 2003-07-10 | Henkel Kgaa | New alkaline protease from Bacillus sp. (DSM 14392) and detergents and cleaning agents containing this new alkaline protease |
DE10343375A1 (en) * | 2003-09-17 | 2005-04-28 | Axaron Bioscience Ag | Method for the detection and analysis of protein-protein interactions |
DE102007032111B4 (en) | 2007-07-09 | 2017-07-20 | Henkel Ag & Co. Kgaa | New proteases and detergents and cleaning agents containing these proteases |
DE102007036756A1 (en) | 2007-08-03 | 2009-02-05 | Henkel Ag & Co. Kgaa | New proteases and detergents and cleaners containing these new proteases |
CN106047892B (en) * | 2016-07-14 | 2019-05-10 | 西南交通大学 | The purification process of Rhizoma Chuanxiong alpha-amylase/subtilisin inhibitor gene and expression product |
WO2018220032A1 (en) * | 2017-05-31 | 2018-12-06 | B.R.A.I.N. Biotechnology Research And Information Network Ag | Optimization of the expression of serine proteases in host cells |
JP7073170B2 (en) * | 2018-04-02 | 2022-05-23 | 花王株式会社 | Liquid detergent composition for tableware and / or hard goods around the kitchen |
EP3785017A4 (en) | 2018-04-27 | 2022-01-26 | Becton, Dickinson and Company | Collection systems for flow cytometrically sorted samples and methods of using the same |
CN113166231A (en) | 2018-10-10 | 2021-07-23 | 诺维信公司 | Chymotrypsin inhibitor variants and uses thereof |
EP3875591A4 (en) * | 2018-10-31 | 2022-09-21 | Qingdao Vland Biotech Group Co. Ltd. | Method for producing washing enzyme having protease resistance |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2000506394A (en) * | 1996-09-24 | 2000-05-30 | ザ、プロクター、エンド、ギャンブル、カンパニー | Stabilized proteinaceous protease inhibitors and variants thereof |
JP2000506393A (en) * | 1996-09-24 | 2000-05-30 | ザ、プロクター、エンド、ギャンブル、カンパニー | Proteases and variants thereof fused with peptide protease inhibitors |
-
1999
- 1999-07-07 AU AU48624/99A patent/AU4862499A/en not_active Abandoned
- 1999-07-07 CA CA002332177A patent/CA2332177A1/en not_active Abandoned
- 1999-07-07 JP JP2000558221A patent/JP2004500008A/en active Pending
- 1999-07-07 WO PCT/US1999/015247 patent/WO2000001831A2/en not_active Application Discontinuation
- 1999-07-07 BR BR9911891-2A patent/BR9911891A/en not_active IP Right Cessation
- 1999-07-07 EP EP99932288A patent/EP1093522A2/en not_active Withdrawn
- 1999-07-07 CN CN 99810503 patent/CN1317052A/en active Pending
- 1999-07-07 AR ARP990103287 patent/AR020105A1/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
JP2004500008A (en) | 2004-01-08 |
CA2332177A1 (en) | 2000-01-13 |
WO2000001831A3 (en) | 2000-04-13 |
EP1093522A2 (en) | 2001-04-25 |
AR020105A1 (en) | 2002-04-10 |
WO2000001831A2 (en) | 2000-01-13 |
CN1317052A (en) | 2001-10-10 |
AU4862499A (en) | 2000-01-24 |
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