BR9911891A - Proteases fused to subtilisin inhibitor variants of streptomyces - Google Patents

Proteases fused to subtilisin inhibitor variants of streptomyces

Info

Publication number
BR9911891A
BR9911891A BR9911891-2A BR9911891A BR9911891A BR 9911891 A BR9911891 A BR 9911891A BR 9911891 A BR9911891 A BR 9911891A BR 9911891 A BR9911891 A BR 9911891A
Authority
BR
Brazil
Prior art keywords
ssi
fusion proteins
proteases
amino acid
acid sequence
Prior art date
Application number
BR9911891-2A
Other languages
Portuguese (pt)
Inventor
Charles Winston Saunders
Original Assignee
Procter & Gamble
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Procter & Gamble filed Critical Procter & Gamble
Publication of BR9911891A publication Critical patent/BR9911891A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4703—Inhibitors; Suppressors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Cosmetics (AREA)

Abstract

Patente de Invenção: <B>"PROTEASES FUNDIDAS COM VARIANTES DE INIBIDOR DE SUBTILISINA DE STREPTOMYCES"<D>. A presente invenção relaciona-se às proteínas de fusão, em que a proteína de fusão compreende uma parte de protease; e uma parte de variante , em que a parte de variante tem uma seq³ência de aminoácidos modificada de uma seq³ência de aminoácidos de origem, em que a seq³ência de amionácidos modificada compreende uma substituição de aminoácido na posição 63 correspondendo ao SSI, e em que a seq³ência de aminoácidos de origem é selecionada do grupo consistindo em SSI, inibidores semelhantes ao SSI, e variantes de inibidores semelhantes ao SSI. Tais proteínas de fusão são úteis em composições de limpeza e em composições para o cuidado pessoal. A presente invenção também relaciona-se às composições de limpeza e às composições para o cuidado pessoal compreendendo as presentes proteínas de fusão, assim como ao DNA codificando as proteínas de fusão.Invention Patent: <B> "PROTEASES MELTED WITH STREPTOMYCES SUBTILISIN INHIBITOR VARIABLES" <D>. The present invention relates to fusion proteins, wherein the fusion protein comprises a protease part; and a variant part, where the variant part has a modified amino acid sequence from a source amino acid sequence, where the modified amion acid sequence comprises an amino acid substitution at position 63 corresponding to the SSI, and where the sequence of origin amino acids is selected from the group consisting of SSI, SSI-like inhibitors, and SSI-like inhibitor variants. Such fusion proteins are useful in cleaning compositions and compositions for personal care. The present invention also relates to cleaning compositions and personal care compositions comprising the present fusion proteins, as well as to the DNA encoding the fusion proteins.

BR9911891-2A 1998-07-07 1999-07-07 Proteases fused to subtilisin inhibitor variants of streptomyces BR9911891A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9190498P 1998-07-07 1998-07-07
PCT/US1999/015247 WO2000001831A2 (en) 1998-07-07 1999-07-07 Proteases fused with variants of streptomyces subtilisin inhibitor

Publications (1)

Publication Number Publication Date
BR9911891A true BR9911891A (en) 2001-03-27

Family

ID=22230236

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9911891-2A BR9911891A (en) 1998-07-07 1999-07-07 Proteases fused to subtilisin inhibitor variants of streptomyces

Country Status (8)

Country Link
EP (1) EP1093522A2 (en)
JP (1) JP2004500008A (en)
CN (1) CN1317052A (en)
AR (1) AR020105A1 (en)
AU (1) AU4862499A (en)
BR (1) BR9911891A (en)
CA (1) CA2332177A1 (en)
WO (1) WO2000001831A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DZ3349A1 (en) 2000-07-28 2002-02-07 Henkel Kgaa NEW AMYLOLYTIC ENZYME FROM BACILLUS SP. A 7-7 (DSM 12368) AND WASHING AND CLEANING PRODUCTS CONTAINING SAID AMYLOLYTIC ENZYME
JP4557108B2 (en) * 2000-08-11 2010-10-06 ライオン株式会社 Chimeric enzyme and detergent composition
WO2002016619A1 (en) * 2000-08-21 2002-02-28 Novozymes A/S Method for production of a protease-inhibitor complex
ES2290184T3 (en) 2000-11-28 2008-02-16 Henkel Kommanditgesellschaft Auf Aktien CYCLODEXTRINE-GLUCANOTRANSPHERASE (CGTASA) FROM BACILLUS AGARADHERENS (DSM 9948) AS WELL AS WASHING AGENTS AND CLEANING IT WITH THIS NEW CYCLODEXTRINE-GLUCANOTRANSPHERASE.
DE10162728A1 (en) 2001-12-20 2003-07-10 Henkel Kgaa New alkaline protease from Bacillus gibsonii (DSM 14393) and washing and cleaning agents containing this new alkaline protease
DE10163884A1 (en) 2001-12-22 2003-07-10 Henkel Kgaa New alkaline protease from Bacillus sp. (DSM 14392) and detergents and cleaning agents containing this new alkaline protease
DE10343375A1 (en) * 2003-09-17 2005-04-28 Axaron Bioscience Ag Method for the detection and analysis of protein-protein interactions
DE102007032111B4 (en) 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa New proteases and detergents and cleaning agents containing these proteases
DE102007036756A1 (en) 2007-08-03 2009-02-05 Henkel Ag & Co. Kgaa New proteases and detergents and cleaners containing these new proteases
CN106047892B (en) * 2016-07-14 2019-05-10 西南交通大学 The purification process of Rhizoma Chuanxiong alpha-amylase/subtilisin inhibitor gene and expression product
KR102641645B1 (en) 2017-05-31 2024-02-27 비.알.에이.아이.엔. 바이오테그놀로지 리서치 앤드 인포메이션 네크워크 아게 Optimization of serine protease expression in host cells
JP7073170B2 (en) * 2018-04-02 2022-05-23 花王株式会社 Liquid detergent composition for tableware and / or hard goods around the kitchen
CN112136034A (en) 2018-04-27 2020-12-25 贝克顿·迪金森公司 Collection system for flow cytometry sorting samples and methods of use thereof
EP3864036A1 (en) 2018-10-10 2021-08-18 Novozymes A/S Chymotrypsin inhibitor variants and the use thereof
CN112368388B (en) * 2018-10-31 2024-01-02 青岛蔚蓝生物集团有限公司 Method for producing protease-resistant washing enzyme

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69722639T2 (en) * 1996-09-24 2004-04-29 The Procter & Gamble Company, Cincinnati STABILIZED PROTEINS WITH PROTEASE INHIBITOR FUNCTION AND VARIANTS OF IT
CN1238805A (en) * 1996-09-24 1999-12-15 普罗格特-甘布尔公司 Proteases and their variants having peptide protease inhibitors fused to them

Also Published As

Publication number Publication date
JP2004500008A (en) 2004-01-08
AU4862499A (en) 2000-01-24
CN1317052A (en) 2001-10-10
WO2000001831A2 (en) 2000-01-13
EP1093522A2 (en) 2001-04-25
WO2000001831A3 (en) 2000-04-13
CA2332177A1 (en) 2000-01-13
AR020105A1 (en) 2002-04-10

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