BR9910463A - Proteìna, dna, vetor recombinante, transporte de microorganismo, e, processo para produzir glutaminase - Google Patents

Proteìna, dna, vetor recombinante, transporte de microorganismo, e, processo para produzir glutaminase

Info

Publication number
BR9910463A
BR9910463A BR9910463-6A BR9910463A BR9910463A BR 9910463 A BR9910463 A BR 9910463A BR 9910463 A BR9910463 A BR 9910463A BR 9910463 A BR9910463 A BR 9910463A
Authority
BR
Brazil
Prior art keywords
glutaminase
dna
protein
recombinant vector
microorganisms
Prior art date
Application number
BR9910463-6A
Other languages
English (en)
Inventor
Kyoko Koibuchi
Hiroaki Nagasaki
Ari Yuasa
Jiro Kataoka
Katsuhiko Kitamoto
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Publication of BR9910463A publication Critical patent/BR9910463A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"PROTEìNA, DNA, VETOR RECOMBINANTE, TRANSFORMANTE DE MICROORGANISMO, E, PROCESSO PARA PRODUZIR GLUTAMINASE" Uma glutaminase é purificada a partir do Aspergillus oryzae e a sua seq³ência parcial de amonoácidos é determinada. Com base nos dados assim obtidos, uma seq³ência parcial do gene da glutaminase é adquirida por PCR. Usando-a como uma sonda, a hidridização é efetuada e desta maneira fragmentos de DNA que contenham o gene de glutaminase são obtidos de uma biblioteca genómica e de uma biblioteca de cDNA do A. oryzae e de uma biblioteca genómica e de uma biblioteca de cDNA do A. nidulans.
BR9910463-6A 1998-05-15 1999-05-12 Proteìna, dna, vetor recombinante, transporte de microorganismo, e, processo para produzir glutaminase BR9910463A (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP13408098 1998-05-15
JP25897498 1998-09-11
JP29244398 1998-10-14
JP8915799 1999-03-30
PCT/JP1999/002455 WO1999060104A1 (fr) 1998-05-15 1999-05-12 Nouvelle glutaminase, son gene et son procede de preparation

Publications (1)

Publication Number Publication Date
BR9910463A true BR9910463A (pt) 2001-01-30

Family

ID=27467607

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9910463-6A BR9910463A (pt) 1998-05-15 1999-05-12 Proteìna, dna, vetor recombinante, transporte de microorganismo, e, processo para produzir glutaminase

Country Status (10)

Country Link
EP (1) EP1077256B1 (pt)
JP (1) JP3918431B2 (pt)
KR (1) KR20010043649A (pt)
CN (1) CN1155702C (pt)
BR (1) BR9910463A (pt)
DE (1) DE69935215T2 (pt)
HK (1) HK1039635B (pt)
ID (1) ID27520A (pt)
MY (1) MY125908A (pt)
WO (1) WO1999060104A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3904185B2 (ja) * 2001-06-21 2007-04-11 キッコーマン株式会社 グルタミナーゼ、グルタミナーゼ遺伝子、新規な組み換え体ｄｎａ及びグルタミナーゼの製造法
US7186540B2 (en) 2001-12-27 2007-03-06 National Institute of Advanced Indusrtial Science and Technology Thermostable glutaminase and thermostable glutaminase gene
DE60335774D1 (de) 2002-11-26 2011-03-03 Ajinomoto Kk Methode für die Produktion von L-Glutamin und L-Glutamin-produzierendes Bakterium
CN103497992B (zh) * 2013-06-28 2015-07-01 天津出入境检验检疫局动植物与食品检测中心 一种新的转基因米曲霉检测试剂盒及其检测方法

Also Published As

Publication number Publication date
JP3918431B2 (ja) 2007-05-23
CN1155702C (zh) 2004-06-30
HK1039635A1 (en) 2002-05-24
EP1077256A4 (en) 2002-05-08
EP1077256A1 (en) 2001-02-21
ID27520A (id) 2001-04-12
WO1999060104A1 (fr) 1999-11-25
MY125908A (en) 2006-08-30
HK1039635B (zh) 2005-03-18
KR20010043649A (ko) 2001-05-25
EP1077256B1 (en) 2007-02-21
CN1309702A (zh) 2001-08-22
DE69935215T2 (de) 2007-09-27
DE69935215D1 (de) 2007-04-05

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Legal Events

Date Code Title Description
B07A Technical examination (opinion): publication of technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 8O, 10 (IX), 24 E 25 DA LPI.

B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.