BR9909356A - Substrato cromogênico que permite detectar a presença da atividade enzimática de pelo menos uma enzima, processo de detecção de uma atividade enzimática por meio de substratos, uso de substrato, e, dispositivo de identificação - Google Patents
Substrato cromogênico que permite detectar a presença da atividade enzimática de pelo menos uma enzima, processo de detecção de uma atividade enzimática por meio de substratos, uso de substrato, e, dispositivo de identificaçãoInfo
- Publication number
- BR9909356A BR9909356A BR9909356-1A BR9909356A BR9909356A BR 9909356 A BR9909356 A BR 9909356A BR 9909356 A BR9909356 A BR 9909356A BR 9909356 A BR9909356 A BR 9909356A
- Authority
- BR
- Brazil
- Prior art keywords
- substrate
- enzymatic activity
- detection
- chromogenic
- enzyme
- Prior art date
Links
- 239000000758 substrate Substances 0.000 title abstract 7
- 230000002255 enzymatic effect Effects 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 5
- 102000004190 Enzymes Human genes 0.000 title abstract 4
- 108090000790 Enzymes Proteins 0.000 title abstract 4
- 239000003593 chromogenic compound Substances 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title abstract 3
- 239000003550 marker Substances 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
- C12Q2334/30—Naphthol derivatives, e.g. alpha-naphthyl-esters, i.e. alpha-NE, beta-naphthyl-esters, i.e. beta-NE
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
Abstract
"SUBSTRATO CROMOGêNICO QUE PERMITE DETECTAR A PRESENçA DA ATIVIDADE ENZIMáTICA DE PELO MENOS UMA ENZIMA, PROCESSO DE DETECçãO DE UMA ATIVIDADE ENZIMáTICA POR MEIO DE SUBSTRATOS, USO DE SUBSTRATO, E, DISPOSITIVO DE IDENTIFICAçãO". A presente invenção refere-se a um substrato cromogênico que permite detectar a presença da atividade enzimática de pelo menos uma enzima. Ela também se refere a um processo de utilização de um tal substrato. Ela ainda se refere a um processo de identificação à base desses substratos e a um dispositivo para a realização desse processo de identificação. A invenção caracteriza-se pelo fato de que o substrato constitui-se de pelo menos duas moléculas, uma primeira molécula constituída de uma parte marcadora não-cromogênica associada a pelo menos uma parte específica alvo da enzima e uma segunda molécula constituída de uma outra substância não-cromogênica, e que a parte marcadora não-cromogênica, uma vez liberada, reage com a segunda molécula para formar uma molécula cromogênica. A invenção é aplicável de preferência no campo da bacteriologia.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9804332A FR2777018B1 (fr) | 1998-04-02 | 1998-04-02 | Nouveau substrat chromogene ainsi que son procede d'utilisation |
PCT/FR1999/000781 WO1999051767A1 (fr) | 1998-04-02 | 1999-04-02 | Nouveaux substrats chromogenes pour la detection d'hydrolases bacteriennes |
Publications (1)
Publication Number | Publication Date |
---|---|
BR9909356A true BR9909356A (pt) | 2000-12-12 |
Family
ID=9524966
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR9909356-1A BR9909356A (pt) | 1998-04-02 | 1999-04-02 | Substrato cromogênico que permite detectar a presença da atividade enzimática de pelo menos uma enzima, processo de detecção de uma atividade enzimática por meio de substratos, uso de substrato, e, dispositivo de identificação |
Country Status (12)
Country | Link |
---|---|
US (1) | US6340573B1 (pt) |
EP (2) | EP1754792B1 (pt) |
JP (1) | JP4382985B2 (pt) |
AT (2) | ATE503842T1 (pt) |
AU (1) | AU753776B2 (pt) |
BR (1) | BR9909356A (pt) |
CA (1) | CA2325626C (pt) |
DE (2) | DE69943326D1 (pt) |
ES (2) | ES2275338T3 (pt) |
FR (1) | FR2777018B1 (pt) |
PL (1) | PL343524A1 (pt) |
WO (1) | WO1999051767A1 (pt) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU7839500A (en) * | 1999-10-01 | 2001-05-10 | Applied Imaging Corporation | Simultaneous enzymatic detection of two analytes |
FR2801903B1 (fr) * | 1999-12-06 | 2004-01-30 | Biomerieux Sa | Nouveaux substrats enzymatiques pour la detection de pseudomonas aeruginosa, compositions les contenant et procede de detection de cette espece |
AU2001296830A1 (en) | 2000-10-14 | 2002-04-29 | Alex M. Saunders | Ligand based solution assay for low concentration analytes |
FR2816956B1 (fr) * | 2000-11-17 | 2004-08-20 | Bio Merieux | Procede et milieu de detection/identification de bacteries a activite esterasique |
FR2903421B1 (fr) | 2006-07-10 | 2008-10-03 | Alain Rambach | Milieu de culture solide pour la detection et/ou la discrimination au niveau de l'espece des enterocoques resistants aux glycopeptides |
AU2007334348B2 (en) | 2006-12-19 | 2013-05-30 | Becton, Dickinson And Company | Chromogenic medium for the detection and identification of vancomycin resistant enterococci and method therefor |
FR2926563B1 (fr) * | 2008-01-21 | 2013-04-26 | Biomerieux Sa | Procede de detection et/ou d'identification de clostridium difficile |
CN102977160B (zh) * | 2012-11-15 | 2016-01-06 | 重庆医科大学 | 一种用4-硝基-1-萘酚或其衍生物为显色团制备β-半乳糖苷酶显色底物的方法及其应用 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1980002433A1 (en) * | 1979-05-02 | 1980-11-13 | Nat Res Dev | The identification of bacteria |
EP0053470B1 (en) * | 1980-12-02 | 1988-01-07 | The Public Health Laboratory Service Board | Method for the estimation of n-acylated primary aromatic amines |
US4588836A (en) * | 1982-09-01 | 1986-05-13 | Toyo Jozo Kabushiki Kaisha | Novel synthetic substrate and assay method using the same |
JPS5963198A (ja) * | 1982-10-01 | 1984-04-10 | Toyo Jozo Co Ltd | 酵素を用いる定量法 |
EP0122028A1 (en) | 1983-03-07 | 1984-10-17 | E-Y Laboratories, Inc. | Colorimetric assay for enzymes, diagnostic article therefor and a method for forming such article |
GB8326696D0 (en) * | 1983-10-05 | 1983-11-09 | Health Lab Service Board | Estimation of salicylates |
EP0150227A1 (en) | 1983-12-23 | 1985-08-07 | FUJIREBIO KABUSHIKI KAISHA also trading as FUJIREBIO INC. | Dipeptide derivatives and their use in determining the activity of carboxypeptidase A |
US4637979A (en) * | 1984-04-06 | 1987-01-20 | Miles Laboratories, Inc. | Composition and test device for determining the presence of leukocytes containing a zwitterion coupling agent |
DE4324392A1 (de) * | 1993-07-21 | 1995-01-26 | Merck Patent Gmbh | Kulturmedium für den gleichzeitigen Nachweis von coliformen Bakterien und Escherichia coli |
FR2708286B1 (fr) * | 1993-07-28 | 1995-10-20 | Rambach Alain | Procédé d'identification de microorganismes avec au moins deux chromogènes. |
US6387650B1 (en) * | 1995-06-07 | 2002-05-14 | Biocontrol Systems, Inc. | Method and composition for detecting bacterial contamination in food products |
-
1998
- 1998-04-02 FR FR9804332A patent/FR2777018B1/fr not_active Expired - Fee Related
-
1999
- 1999-04-02 ES ES99911874T patent/ES2275338T3/es not_active Expired - Lifetime
- 1999-04-02 AT AT06022204T patent/ATE503842T1/de not_active IP Right Cessation
- 1999-04-02 WO PCT/FR1999/000781 patent/WO1999051767A1/fr active IP Right Grant
- 1999-04-02 US US09/646,967 patent/US6340573B1/en not_active Expired - Lifetime
- 1999-04-02 AT AT99911874T patent/ATE343640T1/de not_active IP Right Cessation
- 1999-04-02 BR BR9909356-1A patent/BR9909356A/pt not_active Application Discontinuation
- 1999-04-02 DE DE69943326T patent/DE69943326D1/de not_active Expired - Lifetime
- 1999-04-02 PL PL99343524A patent/PL343524A1/xx not_active Application Discontinuation
- 1999-04-02 EP EP06022204A patent/EP1754792B1/fr not_active Expired - Lifetime
- 1999-04-02 AU AU30406/99A patent/AU753776B2/en not_active Expired
- 1999-04-02 DE DE69933759T patent/DE69933759T2/de not_active Expired - Lifetime
- 1999-04-02 EP EP99911874A patent/EP1066405B1/fr not_active Expired - Lifetime
- 1999-04-02 CA CA2325626A patent/CA2325626C/fr not_active Expired - Lifetime
- 1999-04-02 JP JP2000542478A patent/JP4382985B2/ja not_active Expired - Lifetime
- 1999-04-02 ES ES06022204T patent/ES2361038T3/es not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE69933759T2 (de) | 2007-09-13 |
PL343524A1 (en) | 2001-08-27 |
EP1066405B1 (fr) | 2006-10-25 |
DE69933759D1 (de) | 2006-12-07 |
DE69943326D1 (de) | 2011-05-12 |
FR2777018B1 (fr) | 2003-04-25 |
AU753776B2 (en) | 2002-10-31 |
CA2325626A1 (fr) | 1999-10-14 |
ATE503842T1 (de) | 2011-04-15 |
EP1066405A1 (fr) | 2001-01-10 |
ATE343640T1 (de) | 2006-11-15 |
EP1754792A1 (fr) | 2007-02-21 |
JP2002510503A (ja) | 2002-04-09 |
EP1754792B1 (fr) | 2011-03-30 |
JP4382985B2 (ja) | 2009-12-16 |
CA2325626C (fr) | 2010-03-23 |
ES2275338T3 (es) | 2007-06-01 |
US6340573B1 (en) | 2002-01-22 |
AU3040699A (en) | 1999-10-25 |
ES2361038T3 (es) | 2011-06-13 |
FR2777018A1 (fr) | 1999-10-08 |
WO1999051767A1 (fr) | 1999-10-14 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 24, 25 E 32 DA LPI. |
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B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL. |