BR9908858A - Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the level of expression of a protein, method of obtaining a fragment of nucleic acid and product. - Google Patents

Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the level of expression of a protein, method of obtaining a fragment of nucleic acid and product.

Info

Publication number
BR9908858A
BR9908858A BR9908858-4A BR9908858A BR9908858A BR 9908858 A BR9908858 A BR 9908858A BR 9908858 A BR9908858 A BR 9908858A BR 9908858 A BR9908858 A BR 9908858A
Authority
BR
Brazil
Prior art keywords
nucleic acid
protein
fragment
chimeric gene
host cell
Prior art date
Application number
BR9908858-4A
Other languages
Portuguese (pt)
Inventor
Robert F Cressmann
Stephen M Allen
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BR9908858A publication Critical patent/BR9908858A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

<B>"FRAGMENTO DE áCIDO NUCLéICO ISOLADO, GENE QUIMéRICO, CéLULA HOSPEDEIRA TRANSFORMADA, POLIPEPTìDIO, MéTODO DE ALTERAçãO DO NìVEL DE EXPRESSãO DE UMA PROTEìNA, MéTODO DE OBTENçãO DE UM FRAGMENTO DE áCIDO NUCLéICO E PRODUTO"<D> A presente invenção refere-se a um fragmento de ácido nucléico isolado que codifica uma proteína de fosforilação R1 de amido. A invenção também se refere à construção de um gene quimérico que codifica toda ou uma parte da proteína de fosforilação R1 de amido, na orientação de sentido ou anti-sentido, sendo que a expressão do gene quimérico resulta na produção de níveis alterados da proteína de fosforilação R1 de amido em uma célula hospedeira transformada.<B> "ISOLATED NUCLEIC ACID FRAGMENT, CHIMERIC GENE, TRANSFORMED HOST CELL, POLYPEPTIO, METHOD OF CHANGING THE PROTEIN EXPRESSION LEVEL, METHOD OF GETTING A FOOD PRODUCT". refers to an isolated nucleic acid fragment encoding a starch phosphorylation R1 protein. The invention also relates to the construction of a chimeric gene that encodes all or part of the starch phosphorylation R1 protein, in sense or antisense orientation, the expression of the chimeric gene resulting in the production of altered levels of the protein of starch phosphorylation R1 of starch in a transformed host cell.

BR9908858-4A 1998-04-09 1999-04-08 Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the level of expression of a protein, method of obtaining a fragment of nucleic acid and product. BR9908858A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8114398P 1998-04-09 1998-04-09
PCT/US1999/007639 WO1999053072A1 (en) 1998-04-09 1999-04-08 Starch r1 phosphorylation protein homologs

Publications (1)

Publication Number Publication Date
BR9908858A true BR9908858A (en) 2000-12-19

Family

ID=22162353

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9908858-4A BR9908858A (en) 1998-04-09 1999-04-08 Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the level of expression of a protein, method of obtaining a fragment of nucleic acid and product.

Country Status (4)

Country Link
EP (1) EP1068333A1 (en)
AU (1) AU3478499A (en)
BR (1) BR9908858A (en)
WO (1) WO1999053072A1 (en)

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