BR9907966A - Nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the expression level of an lcb1 subunit of serine palmitoyl transferase in a host cell, method of obtaining a nucleic acid fragment, product and method of evaluation of at least one compound for its ability to inhibit the activity of a lcb1 subunit of serine palmitoyl transferase - Google Patents

Nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the expression level of an lcb1 subunit of serine palmitoyl transferase in a host cell, method of obtaining a nucleic acid fragment, product and method of evaluation of at least one compound for its ability to inhibit the activity of a lcb1 subunit of serine palmitoyl transferase

Info

Publication number
BR9907966A
BR9907966A BR9907966-6A BR9907966A BR9907966A BR 9907966 A BR9907966 A BR 9907966A BR 9907966 A BR9907966 A BR 9907966A BR 9907966 A BR9907966 A BR 9907966A
Authority
BR
Brazil
Prior art keywords
nucleic acid
host cell
acid fragment
lcb1
palmitoyl transferase
Prior art date
Application number
BR9907966-6A
Other languages
Portuguese (pt)
Inventor
Rebecca E Cahoon
Anthony J Kinney
J Antoni Rafalski
Alan R Rendina
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BR9907966A publication Critical patent/BR9907966A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"FRAGMENTO DE áCIDO NUCLéICO, GENE QUIMéRICO, CéLULA HOSPEDEIRA TRANSFORMADA, POLIPEPTìDEO, MéTODO DE ALTERAçãO DO NìVEL DE EXPRESSãO DE UMA SUBUNIDADE Lcb1 DE SERINA PALMITOIL TRANSFERASE EM UMA CéLULA HOSPEDEIRA, MéTODO DE OBTENçãO DE UM FRAGMENTO DE áCIDO NUCLéICO, PRODUTO E MéTODO DE AVALIAçãO DE PELO MENOS UM COMPOSTO QUANTO à SUA CAPACIDADE DE INIBIR A ATIVIDADE DE UMA SUBUNIDADE Lcb1 DE SERINA PALMITOIL TRANSFERASE" A presente invenção refere-se a um fragmento de ácido nucléico isolado que codifica uma subunidade Lcb1 de serina palmitoil transferase. A invenção também se refere à construção de um gene quimérico que codifica toda ou uma parte da subunidade Lcb1 de serina palmitoil transferase, na orientação de sentido ou de anti-sentido, sendo que a expressão do gene quimérico resulta na produção de níveis alterados da subunidade Lcb1 de serina palmitoil transferase em uma célula hospedeira transformada"FRAGMENT OF NUCLEIC ACID, CHEMICAL GENE, TRANSFORMED HOST CELL, POLYPEPTIOUS, METHOD OF ALTERING THE EXPRESSION LEVEL OF A PALMITOIL SERINE LUCB1 SUBUNITY OF A HOSPITAL, ANTHEMOUS HEROPHETIC CELL AT LEAST ONE COMPOUND AS TO ITS ABILITY TO INHIBIT THE ACTIVITY OF A Lcb1 SUBUNITY OF SERINE PALMITOYL TRANSFERASE "The present invention relates to an isolated nucleic acid fragment encoding an Lcb1 subunit of palmitoyl serine transferase. The invention also relates to the construction of a chimeric gene that encodes all or part of the Lcb1 subunit of serine palmitoyl transferase, in sense or antisense orientation, the expression of the chimeric gene resulting in the production of altered levels of the subunit Lcb1 of serine palmitoyl transferase in a transformed host cell

BR9907966-6A 1998-03-26 1999-03-19 Nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the expression level of an lcb1 subunit of serine palmitoyl transferase in a host cell, method of obtaining a nucleic acid fragment, product and method of evaluation of at least one compound for its ability to inhibit the activity of a lcb1 subunit of serine palmitoyl transferase BR9907966A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7943098P 1998-03-26 1998-03-26
PCT/US1999/006045 WO1999049053A1 (en) 1998-03-26 1999-03-19 Lcb1 subunit of serine palmitoyltransferase

Publications (1)

Publication Number Publication Date
BR9907966A true BR9907966A (en) 2000-12-12

Family

ID=22150493

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9907966-6A BR9907966A (en) 1998-03-26 1999-03-19 Nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the expression level of an lcb1 subunit of serine palmitoyl transferase in a host cell, method of obtaining a nucleic acid fragment, product and method of evaluation of at least one compound for its ability to inhibit the activity of a lcb1 subunit of serine palmitoyl transferase

Country Status (4)

Country Link
EP (1) EP1066387A1 (en)
AU (1) AU3103399A (en)
BR (1) BR9907966A (en)
WO (1) WO1999049053A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7659450B2 (en) * 1998-03-26 2010-02-09 E. I. Du Pont De Nemours And Company Lcb1 subunit of serine palmitoyltransferase

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5583030A (en) * 1992-06-30 1996-12-10 The University Of Kentucky Research Foundation Method for obtaining antifungal and herbicidal compounds that target the first committed step in shingolipid long-chain base biosynthesis

Also Published As

Publication number Publication date
EP1066387A1 (en) 2001-01-10
AU3103399A (en) 1999-10-18
WO1999049053A1 (en) 1999-09-30

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