BR9907980A - Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide lcb2 subunit, method of altering the expression level of an lcb2 subunit, method of obtaining a nucleic acid fragment, product and method of evaluation of the ability of at least one compound to inhibit the activity of an lcb2 subunit - Google Patents

Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide lcb2 subunit, method of altering the expression level of an lcb2 subunit, method of obtaining a nucleic acid fragment, product and method of evaluation of the ability of at least one compound to inhibit the activity of an lcb2 subunit

Info

Publication number
BR9907980A
BR9907980A BR9907980-1A BR9907980A BR9907980A BR 9907980 A BR9907980 A BR 9907980A BR 9907980 A BR9907980 A BR 9907980A BR 9907980 A BR9907980 A BR 9907980A
Authority
BR
Brazil
Prior art keywords
lcb2
nucleic acid
acid fragment
lcb2 subunit
subunit
Prior art date
Application number
BR9907980-1A
Other languages
Portuguese (pt)
Inventor
Edgar B Cahoon
Rebecca E Cahoon
Anthony J Kinney
J Antoni Rafalski
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BR9907980A publication Critical patent/BR9907980A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

''FRAGMENTO DE áCIDO NUCLéICO ISOLADO, GENE QUIMéRICO, CéLULA HOSPEDEIRA TRANSFORMADA, SUB-UNIDADE DE Lcb2 DE POLIPEPTìDEO, MéTODO DE ALTERAçãO DO NìVEL DE EXPRESSãO DE UMA SUB-UNIDADE Lcb2, MéTODO DE OBTENçãO DE UM FRAGMENTO DE áCIDO NUCLéICO, PRODUTO E MéTODO DE AVALIAçãO DA CAPACIDADE DE PELO MENOS UM COMPOSTO EM INIBIR A ATIVIDADE DE UMA SUB-UNIDADE Lcb2" A presente invenção refere-se a um fragmento de ácido nucléico isolado codificador de uma sub-unidade Lcb2 de palmitoiltransferase de serina. A presente invenção também se refere à construção de um gene quimérico que codifique a sub-unidade Lcb2 de palmitoiltransferase de serina, no todo ou em parte, em orientação de mesmo sentido ou sentido contrário, em que a expressão do gene quimérico resulte na produção de níveis alterados da sub-unidade Lcb2 de palmitoiltransferase de serina em uma célula hospedeira transformada.'' ISOLATED NUCLEIC ACID FRAGMENT, CHIMERIC GENE, TRANSFORMED HOST CELL, POLYPEPTIDE Lcb2 SUB-UNIT, METHOD OF ALTERING THE EXPRESSION LEVEL OF A Lcb SUB-UNIT, METHOD OF METHOD, OF EVALUATION OF THE CAPACITY OF AT LEAST ONE COMPOUND IN INHIBITING THE ACTIVITY OF AN Lcb2 SUB-UNIT "The present invention relates to an isolated nucleic acid fragment encoding an Lcb2 sub-unit of serine palmitoyltransferase. The present invention also refers to the construction of a chimeric gene encoding the Lcb2 subunit of serine palmitoyltransferase, in whole or in part, in the same or opposite direction, in which the expression of the chimeric gene results in the production of altered levels of the sub- Lcb2 unit of serine palmitoyltransferase in a transformed host cell.

BR9907980-1A 1998-03-26 1999-03-22 Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide lcb2 subunit, method of altering the expression level of an lcb2 subunit, method of obtaining a nucleic acid fragment, product and method of evaluation of the ability of at least one compound to inhibit the activity of an lcb2 subunit BR9907980A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7946298P 1998-03-26 1998-03-26
PCT/US1999/006587 WO1999049021A1 (en) 1998-03-26 1999-03-22 Lcb2 subunit of serine palmitoyltransferase

Publications (1)

Publication Number Publication Date
BR9907980A true BR9907980A (en) 2000-11-28

Family

ID=22150719

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9907980-1A BR9907980A (en) 1998-03-26 1999-03-22 Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide lcb2 subunit, method of altering the expression level of an lcb2 subunit, method of obtaining a nucleic acid fragment, product and method of evaluation of the ability of at least one compound to inhibit the activity of an lcb2 subunit

Country Status (4)

Country Link
EP (1) EP1064361A1 (en)
AU (1) AU3204799A (en)
BR (1) BR9907980A (en)
WO (1) WO1999049021A1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4902620A (en) * 1985-04-25 1990-02-20 Eli Lilly And Company Novel DNA for expression of delta-aminolevulinic acid synthetase and related method
US5583030A (en) * 1992-06-30 1996-12-10 The University Of Kentucky Research Foundation Method for obtaining antifungal and herbicidal compounds that target the first committed step in shingolipid long-chain base biosynthesis
DE19650216A1 (en) * 1996-12-04 1998-06-10 Inst Pflanzengenetik & Kultur Process for influencing chlorophyll biosynthesis in plants

Also Published As

Publication number Publication date
EP1064361A1 (en) 2001-01-03
AU3204799A (en) 1999-10-18
WO1999049021A1 (en) 1999-09-30

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Legal Events

Date Code Title Description
FA10 Dismissal: dismissal - article 33 of industrial property law
B11Y Definitive dismissal acc. article 33 of ipl - extension of time limit for request of examination expired