BR9813323A - Processos para preparar plantas masculinas estéreis, para a restauração de fertilidade em uma planta masculina estéril, para produzir uma linhagem masculina estéril homozigótica, e para a produção de plantas hìbridas férteis, plantas hìbridas férteis, vetor, cepa de agrobacterium, plantas transformadas com a cepa de agrobacterium, masculina estéril, e, linhagem masculina estéril homozigótica - Google Patents

Processos para preparar plantas masculinas estéreis, para a restauração de fertilidade em uma planta masculina estéril, para produzir uma linhagem masculina estéril homozigótica, e para a produção de plantas hìbridas férteis, plantas hìbridas férteis, vetor, cepa de agrobacterium, plantas transformadas com a cepa de agrobacterium, masculina estéril, e, linhagem masculina estéril homozigótica

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Publication number
BR9813323A
BR9813323A BR9813323-3A BR9813323A BR9813323A BR 9813323 A BR9813323 A BR 9813323A BR 9813323 A BR9813323 A BR 9813323A BR 9813323 A BR9813323 A BR 9813323A
Authority
BR
Brazil
Prior art keywords
sterile male
plants
male
sterile
tpp
Prior art date
Application number
BR9813323-3A
Other languages
English (en)
Inventor
Cornelis Maria Petrus Van Dun
Oscar Johannes Maria Goddijn
Original Assignee
Mogen Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mogen Int filed Critical Mogen Int
Publication of BR9813323A publication Critical patent/BR9813323A/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"PROCESSOS PARA PREPARAR PLANTAS MASCULINAS ESTéREIS, PARA A RESTAURAçãO DE FERTILIDADE EM UMA PLANTA MASCULINA ESTéRIL, PARA PRODUZIR UMA LINHAGEM MASCULINA ESTéRIL HOMOZIGóTICA, E PARA A PRODUçãO DE PLANTAS HìBRIDAS FéRTEIS, PLANTAS HìBRIDAS FéRTEIS, VETOR, CEPA DE AGROBACTERIUM, PLANTAS TRANSFORMADAS COM A CEPA DE AGROBACTERIUM, MASCULINA ESTéRIL, E, LINHAGEM MASCULINA ESTéRIL HOMOZIGóTICA". A presente invenção está direcionada para a produção de plantas masculinas estéreis dotando-se as mesmas com um DNA recombinante capaz de expressão específica, no sistema reprodutivo masculino de uma planta, da enzima trealose fosfato fosfatase (TPP). A restauração da fertilidade pode ser estabelecida dotando-se as referidas plantas masculinas estéreis com um DNA recombinante capaz de expressão de trealose fosfato sintase (TPS) sob o controle de um promotor induzível ou com um DNA recombinante capaz de expressão de uma proteína supressora que suprime a expressão da TPP sob o controle de um promotor induzível. Esta(s) possibilidade(s) de restauração induzível permite(m) a manutenção de uma linhagem masculina estéril homozigótica. A restauração também pode ser realizada pulverizando-se as plantas masculinas estéreis com ácido giberelínico. Para a produção de híbridos ou de sementes híbridas proporciona-se um sistema de recombinação específica-para-sítio inserindo-se dois sítios de recombinação esecíficos-para-sítio flanqueando o DNA recombinante codificando para TPP e cruzando as linhas masculinas estéreis com linhas que expressam a recombinase correspondente. Cruzando-se a recombinase obtém-se a excisão do gene codificante para TPP e produz-se híbridos férteis. específicos-para-sítio flanqueando o DNA recombinante codificando para TPP e cruzando as linhas masculinas estéreis com linhas que expressam a recombinase correspondente. Cruzando-se a recombinase obtém-se a excisão do gene codificante para TPP e produz-se híbridos férteis.
BR9813323-3A 1997-10-30 1998-10-30 Processos para preparar plantas masculinas estéreis, para a restauração de fertilidade em uma planta masculina estéril, para produzir uma linhagem masculina estéril homozigótica, e para a produção de plantas hìbridas férteis, plantas hìbridas férteis, vetor, cepa de agrobacterium, plantas transformadas com a cepa de agrobacterium, masculina estéril, e, linhagem masculina estéril homozigótica BR9813323A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97203373 1997-10-30
PCT/EP1998/007008 WO1999023233A1 (en) 1997-10-30 1998-10-30 Nuclear male sterile plants, method of producing same and methods to restore fertility

Publications (1)

Publication Number Publication Date
BR9813323A true BR9813323A (pt) 2000-08-22

Family

ID=8228888

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9813323-3A BR9813323A (pt) 1997-10-30 1998-10-30 Processos para preparar plantas masculinas estéreis, para a restauração de fertilidade em uma planta masculina estéril, para produzir uma linhagem masculina estéril homozigótica, e para a produção de plantas hìbridas férteis, plantas hìbridas férteis, vetor, cepa de agrobacterium, plantas transformadas com a cepa de agrobacterium, masculina estéril, e, linhagem masculina estéril homozigótica

Country Status (14)

Country Link
US (2) US6603064B1 (pt)
EP (1) EP1027448A1 (pt)
JP (1) JP2002507381A (pt)
KR (1) KR20010031655A (pt)
CN (1) CN1280625A (pt)
AR (1) AR017534A1 (pt)
AU (1) AU739946B2 (pt)
BR (1) BR9813323A (pt)
CA (1) CA2307956A1 (pt)
HU (1) HUP0004084A3 (pt)
IL (1) IL135873A0 (pt)
MA (1) MA25238A1 (pt)
WO (1) WO1999023233A1 (pt)
ZA (1) ZA989886B (pt)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL343460A1 (en) * 1998-03-11 2001-08-13 Novartis Ag Expression of trehalose biosynthetic genes in plants
US6852911B1 (en) * 1999-08-31 2005-02-08 Fertiseed Ltd. Method of producing a male sterile plant by exogenic allelism
CA2449810A1 (en) * 2001-06-07 2002-12-12 National Research Council Of Canada Anther-specific taa1 genes encoding fatty acyl co-a reductases, and uses thereof
US7129398B2 (en) * 2001-11-21 2006-10-31 Monsanto Technology Llc Reversible male sterility in transgenic plants by expression of a GA-insensitive mutant protein, gai.
WO2004018639A2 (en) * 2002-08-23 2004-03-04 Basf Plant Science Gmbh Male sterility restoration as a selectable marker in plant transformation
EP1742527B1 (en) * 2004-04-20 2013-02-20 Syngenta Participations AG Regulatory sequences for expressing gene products in plant reproductive tissue
WO2006060376A2 (en) * 2004-12-03 2006-06-08 Syngenta Participations Ag Stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase
AU2008345643A1 (en) * 2007-12-19 2009-07-09 Progeny Advanced Genetics Intermarket class hybrid lettuce
CN102257143B (zh) 2008-11-28 2013-11-06 科学及工业研究委员会 生产雄性不育植物的方法
NL2004412C2 (en) * 2010-03-16 2011-09-20 Nickerson Zwaan B V Male sterile leek plants.
CN111235163B (zh) * 2020-03-20 2022-05-31 南京农业大学 水稻减数分裂发育相关基因OsMFS1及其应用
CN117247967B (zh) * 2023-11-10 2024-02-20 北京首佳利华科技有限公司 雄性不育基因ZmPKSA及其在创制玉米雄性不育系中的应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9117634D0 (en) * 1991-08-15 1991-10-02 Evans Lloyd T Method of treating plants or plant tissues
FI943133A0 (fi) * 1994-06-29 1994-06-29 Alko Ab Oy Transgena vaexter
CN1196655A (zh) 1995-09-21 1998-10-21 华盛顿州立大学研究基金会 条件雄性可育植物以及用于恢复其育性的方法和组合物
JPH11514233A (ja) 1995-10-13 1999-12-07 パーデュー・リサーチ・ファウンデーション ハイブリッド植物の生産方法
EP0784095A3 (en) * 1996-01-12 1997-12-29 Mogen International N.V. Enhanced accummulation of trehalose in plants
AUPN824196A0 (en) 1996-02-22 1996-03-14 Gene Shears Pty. Limited Male sterile plants
IN1997CH00924A (en) * 1996-05-03 2005-03-04 Syngenta Mogen Bv Regulating metabolism by modifying the level of trehalose-6-phosphate

Also Published As

Publication number Publication date
MA25238A1 (fr) 2001-10-01
JP2002507381A (ja) 2002-03-12
AU1157799A (en) 1999-05-24
HUP0004084A2 (en) 2001-03-28
US20040064857A1 (en) 2004-04-01
EP1027448A1 (en) 2000-08-16
WO1999023233A1 (en) 1999-05-14
HUP0004084A3 (en) 2002-11-28
AU739946B2 (en) 2001-10-25
ZA989886B (en) 1999-04-30
CN1280625A (zh) 2001-01-17
AR017534A1 (es) 2001-09-12
US6603064B1 (en) 2003-08-05
IL135873A0 (en) 2001-05-20
KR20010031655A (ko) 2001-04-16
CA2307956A1 (en) 1999-05-14

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