BR9810171A - Fructose 1,6 bisphosphate aldolase expression in transgenic plants - Google Patents

Fructose 1,6 bisphosphate aldolase expression in transgenic plants

Info

Publication number
BR9810171A
BR9810171A BR9810171-4A BR9810171A BR9810171A BR 9810171 A BR9810171 A BR 9810171A BR 9810171 A BR9810171 A BR 9810171A BR 9810171 A BR9810171 A BR 9810171A
Authority
BR
Brazil
Prior art keywords
fructose
fda
transgenic plants
starch
leaf
Prior art date
Application number
BR9810171-4A
Other languages
Portuguese (pt)
Inventor
Gerard F Barry
Nordine Cheikh
Ganesh M Kishore
Original Assignee
Monsanto Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Co filed Critical Monsanto Co
Publication of BR9810171A publication Critical patent/BR9810171A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

Patente de Invenção: "EXPRESSãO DE FRUTOSE 1,6 BIFOSFATO ALDOLASE EM PLANTAS TRANSGêNICAS". A frutose-1,6-bisfosfato aldolase (fda) é uma enzima que catalisa inversamente a reação de conversão de triosefosfato em frutose-1,6-bisfosfato. Na folha, esta enzima encontra-se localizada no cloroplasto (síntese de amido) e no citosol (biossíntese de sacarose). Foram geradas plantas transgênicas que expressam o gene E.coli fda no cloroplasto para aperfeiçoar o rendimento da planta, especificamente aumentando a capacidade biossintética do amido da folha e a produção de sacarose em geral. As folhas de plantas que expressam o transgene fda mostraram um acúmulo de amido significativamente maior, quando comparadas às plantas de controle expressando um vetor nulo, especificamente de forma precoce no fotoperíodo, porém possuíam sacarose inferior na folha. As plantas transgênicas também possuíam uma massa de raiz significativamente maior. Adicionalmente, as batatas transgênicas expressando fda exibiram uniformidade de sólidos aperfeiçoada.Invention Patent: "EXPRESSION OF FRUCTOSE 1.6 BIPOSPHATE ALDOLASE IN TRANSGENIC PLANTS". Fructose-1,6-bisphosphate aldolase (fda) is an enzyme that inversely catalyzes the reaction of converting triosphosphate to fructose-1,6-bisphosphate. In the leaf, this enzyme is located in the chloroplast (synthesis of starch) and in the cytosol (biosynthesis of sucrose). Transgenic plants that express the E.coli fda gene in the chloroplast were generated to improve plant yield, specifically by increasing the biosynthetic capacity of leaf starch and the production of sucrose in general. The leaves of plants expressing the fda transgene showed a significantly greater accumulation of starch, when compared to control plants expressing a null vector, specifically early in the photoperiod, but had lower sucrose in the leaf. Transgenic plants also had a significantly larger root mass. In addition, transgenic potatoes expressing fda exhibited improved solid uniformity.

BR9810171-4A 1997-06-17 1998-06-16 Fructose 1,6 bisphosphate aldolase expression in transgenic plants BR9810171A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4995597P 1997-06-17 1997-06-17
PCT/US1998/012447 WO1998058069A1 (en) 1997-06-17 1998-06-16 Expression of fructose 1,6 bisphosphate aldolase in transgenic plants

Publications (1)

Publication Number Publication Date
BR9810171A true BR9810171A (en) 2001-11-06

Family

ID=21962657

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9810171-4A BR9810171A (en) 1997-06-17 1998-06-16 Fructose 1,6 bisphosphate aldolase expression in transgenic plants

Country Status (16)

Country Link
EP (1) EP1002114A1 (en)
JP (1) JP2001520522A (en)
CN (1) CN1267333A (en)
AR (1) AR020307A1 (en)
AU (1) AU735383B2 (en)
BR (1) BR9810171A (en)
CA (1) CA2294525A1 (en)
EA (1) EA200000029A1 (en)
HU (1) HUP0004228A3 (en)
IL (1) IL133532A0 (en)
NO (1) NO996218L (en)
NZ (1) NZ501781A (en)
PL (1) PL337581A1 (en)
TR (1) TR199903157T2 (en)
WO (1) WO1998058069A1 (en)
ZA (1) ZA985261B (en)

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US6512162B2 (en) 1998-07-10 2003-01-28 Calgene Llc Expression of eukaryotic peptides in plant plastids
ATE360072T1 (en) * 1998-07-10 2007-05-15 Calgene Llc EXPRESSION OF EUKAROTIC PEPTIDES IN PLANT PLASTIDS
US7345217B2 (en) 1998-09-22 2008-03-18 Mendel Biotechnology, Inc. Polynucleotides and polypeptides in plants
US7858848B2 (en) 1999-11-17 2010-12-28 Mendel Biotechnology Inc. Transcription factors for increasing yield
US7868229B2 (en) 1999-03-23 2011-01-11 Mendel Biotechnology, Inc. Early flowering in genetically modified plants
JP3357909B2 (en) 1999-03-10 2002-12-16 奈良先端科学技術大学院大学長 How to increase productivity of higher plants
WO2001036597A1 (en) * 1999-11-17 2001-05-25 Mendel Biotechnology, Inc. Plant biochemistry-related genes
CN104531702A (en) 1999-12-16 2015-04-22 孟山都技术有限公司 Novel plant expression constructs
CA2425349C (en) 2000-10-30 2011-08-02 Monsanto Technology Llc Canola event pv-bngt04(rt73) and compositions and methods for detection thereof
US7939715B2 (en) 2000-11-16 2011-05-10 Mendel Biotechnology, Inc. Plants with improved yield and stress tolerance
US8426678B2 (en) 2002-09-18 2013-04-23 Mendel Biotechnology, Inc. Polynucleotides and polypeptides in plants
JP2005130770A (en) * 2003-10-30 2005-05-26 Ajinomoto Co Inc Potato increased in starch level obtained per plant body and method for creating the same
AU2005268943B2 (en) * 2004-07-31 2011-04-14 Metanomics Gmbh Preparation of organisms with faster growth and/or higher yield
EP3318271A1 (en) * 2005-11-16 2018-05-09 Pro Natura Gesellschaft für Gesunde Ernährung mbH Agent for use in the case of fructose intolerance
JP5205563B2 (en) * 2006-02-09 2013-06-05 独立行政法人科学技術振興機構 Plant having improved growth and disease resistance, and method for producing the same
CN102268430B (en) * 2010-06-02 2013-05-15 中国科学院大连化学物理研究所 Fructose-1,6-diphosphate aldolase promoter and application thereof, construct and vector
EP3337923B2 (en) 2015-09-21 2023-01-04 Modern Meadow, Inc. Fiber reinforced tissue composites
BR112017016266A2 (en) 2016-02-15 2018-03-27 Modern Meadow, Inc. method for producing a biofabricated material.
CN105973851B (en) * 2016-06-12 2018-10-23 中国科学院地球化学研究所 A kind of bathmic method of quantitative determination plant photosynthesis
CA3008850A1 (en) 2017-06-29 2018-12-29 Modern Meadow, Inc. Yeast strains and methods for producing collagen
AU2018253595A1 (en) 2017-11-13 2019-05-30 Modern Meadow, Inc. Biofabricated leather articles having zonal properties
EP3704202A4 (en) 2019-01-17 2020-12-16 Modern Meadow, Inc. Layered collagen materials and methods of making the same
CN112094859B (en) * 2020-09-25 2022-01-25 扬州大学 Paeonia ostii PoFBA gene, expression vector, preparation method and application thereof
CN112094851B (en) * 2020-09-25 2022-01-25 扬州大学 Paeonia ostii PoCAB151 gene, expression vector, and preparation method and application thereof
CN113528567B (en) * 2021-07-22 2022-05-03 中国林业科学研究院林业研究所 Use of FBA8 protein or protein derived therefrom for regulating vascular bundle division and/or rachis cross-sectional area in plants
CN113528497B (en) * 2021-07-22 2022-06-24 中国林业科学研究院林业研究所 Use of FBA8 gene or FBA8 protein for preparing reagent with transphosphorylation activity and/or proteolytic activity
CN114920938B (en) * 2022-05-29 2023-11-10 深圳绿天琪生物医药有限公司 Preparation and application of arginine coupled plant source glycan and polypeptide synthetic copolymer

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JP3325022B2 (en) * 1990-06-18 2002-09-17 モンサント カンパニー Increased starch content in plants
DE19502053A1 (en) * 1995-01-13 1996-07-18 Inst Genbiologische Forschung Processes and DNA molecules to increase the rate of photosynthesis in plants, as well as plant cells and plants with an increased rate of photosynthesis
US5716837A (en) * 1995-02-10 1998-02-10 Monsanto Company Expression of sucrose phosphorylase in plants

Also Published As

Publication number Publication date
EP1002114A1 (en) 2000-05-24
AR020307A1 (en) 2002-05-08
CA2294525A1 (en) 1998-12-23
PL337581A1 (en) 2000-08-28
EA200000029A1 (en) 2000-08-28
WO1998058069A1 (en) 1998-12-23
CN1267333A (en) 2000-09-20
NO996218L (en) 2000-02-14
AU735383B2 (en) 2001-07-05
NO996218D0 (en) 1999-12-15
AU8144598A (en) 1999-01-04
NZ501781A (en) 2001-07-27
IL133532A0 (en) 2001-04-30
HUP0004228A2 (en) 2001-03-28
JP2001520522A (en) 2001-10-30
ZA985261B (en) 1999-01-07
TR199903157T2 (en) 2000-06-21
HUP0004228A3 (en) 2002-11-28

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