AR020307A1 - DOUBLE CHAIN RECOMBINANT DNA MOLECULA, TRANSGENIC VEGETABLE CELL CONTAINING IT, FOOD PRODUCT, PROPAGATION MATERIAL AND PROCESS TO INCREASE THE PHOTOSYNTHESIS RATE PERFORMANCE, GROWTH AND UNIFORMITY OF PLANTES AND METHODS. - Google Patents

DOUBLE CHAIN RECOMBINANT DNA MOLECULA, TRANSGENIC VEGETABLE CELL CONTAINING IT, FOOD PRODUCT, PROPAGATION MATERIAL AND PROCESS TO INCREASE THE PHOTOSYNTHESIS RATE PERFORMANCE, GROWTH AND UNIFORMITY OF PLANTES AND METHODS.

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Publication number
AR020307A1
AR020307A1 ARP980102895A ARP980102895A AR020307A1 AR 020307 A1 AR020307 A1 AR 020307A1 AR P980102895 A ARP980102895 A AR P980102895A AR P980102895 A ARP980102895 A AR P980102895A AR 020307 A1 AR020307 A1 AR 020307A1
Authority
AR
Argentina
Prior art keywords
express
recombinant dna
uniformity
leaves
fda
Prior art date
Application number
ARP980102895A
Other languages
Spanish (es)
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Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of AR020307A1 publication Critical patent/AR020307A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Dicha molécula ADN recombinante contiene: a)un promotor funcional en células vegetales y b)una secuencia de ADN que codifica un polipéptido que presenteactividad enzimática de una fructosa -1,6-difosfato aldolasa y ligada operativamente con el promotor con orientacion en el sentido del marco de lectura. Lafructosa-1,6-difosfato aldolasa (FDA) es un enzima que cataliza de forma reversible la reaccion que convierte triosafosfato en fructosa-1,6-difosfato. En lahoja esta enzima está localizada en el cloroplasto (síntesis de almidon) y en el citosol (biosíntesis de sacarosa). Se generaron plantas transgénicas queexpresan el gen fda de E. Coli en el cloroplasto para mejorar el rendimiento de la planta incrementando la capacidad de biosíntesis dealmidon en hojas enparticular y produccion de sacarosa en general. Las hojas de plantas que expresan el transgen fda mostraron una acumulacion significativa mayor de almidon encomparacion con plantas control que expresan un vector nulo, en aprticular en la fase temprana del fotoperíodo, pero presentaron un bajo contenido de sacarosaen las hojas. Las plantas transgénicas también presentaban una masa de raíces significativamente mayor. Aun más, las papas transgénicas que expresan fdamostraron unamayor uniformidad de solidos.Said recombinant DNA molecule contains: a) a functional promoter in plant cells and b) a DNA sequence encoding a polypeptide that exhibits enzymatic activity of a fructose -1,6-diphosphate aldolase and operably linked with the promoter oriented in the sense of the frame Reading. Lafructose-1,6-diphosphate aldolase (FDA) is an enzyme that reversibly catalyzes the reaction that converts triosaphosphate into fructose-1,6-diphosphate. In the leaf this enzyme is located in the chloroplast (starch synthesis) and in the cytosol (sucrose biosynthesis). Transgenic plants were generated that express the E. coli fda gene in the chloroplast to improve the plant's performance by increasing the capacity of dealmidon biosynthesis in particular leaves and sucrose production in general. The leaves of plants that express the transgene fda showed a significant accumulation of starch in comparison with control plants that express a null vector, in aprticular in the early phase of the photoperiod, but presented a low sucrose content in the leaves. The transgenic plants also had a significantly greater root mass. Furthermore, the transgenic potatoes they express showed greater uniformity of solids.

ARP980102895A 1997-06-17 1998-06-17 DOUBLE CHAIN RECOMBINANT DNA MOLECULA, TRANSGENIC VEGETABLE CELL CONTAINING IT, FOOD PRODUCT, PROPAGATION MATERIAL AND PROCESS TO INCREASE THE PHOTOSYNTHESIS RATE PERFORMANCE, GROWTH AND UNIFORMITY OF PLANTES AND METHODS. AR020307A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US4995597P 1997-06-17 1997-06-17

Publications (1)

Publication Number Publication Date
AR020307A1 true AR020307A1 (en) 2002-05-08

Family

ID=21962657

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP980102895A AR020307A1 (en) 1997-06-17 1998-06-17 DOUBLE CHAIN RECOMBINANT DNA MOLECULA, TRANSGENIC VEGETABLE CELL CONTAINING IT, FOOD PRODUCT, PROPAGATION MATERIAL AND PROCESS TO INCREASE THE PHOTOSYNTHESIS RATE PERFORMANCE, GROWTH AND UNIFORMITY OF PLANTES AND METHODS.

Country Status (16)

Country Link
EP (1) EP1002114A1 (en)
JP (1) JP2001520522A (en)
CN (1) CN1267333A (en)
AR (1) AR020307A1 (en)
AU (1) AU735383B2 (en)
BR (1) BR9810171A (en)
CA (1) CA2294525A1 (en)
EA (1) EA200000029A1 (en)
HU (1) HUP0004228A3 (en)
IL (1) IL133532A0 (en)
NO (1) NO996218L (en)
NZ (1) NZ501781A (en)
PL (1) PL337581A1 (en)
TR (1) TR199903157T2 (en)
WO (1) WO1998058069A1 (en)
ZA (1) ZA985261B (en)

Families Citing this family (27)

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EP1097211B1 (en) * 1998-07-10 2007-04-18 Calgene LLC Expression of eukaryotic peptides in plant plastids
US6512162B2 (en) 1998-07-10 2003-01-28 Calgene Llc Expression of eukaryotic peptides in plant plastids
US7868229B2 (en) 1999-03-23 2011-01-11 Mendel Biotechnology, Inc. Early flowering in genetically modified plants
US7345217B2 (en) 1998-09-22 2008-03-18 Mendel Biotechnology, Inc. Polynucleotides and polypeptides in plants
US7858848B2 (en) 1999-11-17 2010-12-28 Mendel Biotechnology Inc. Transcription factors for increasing yield
JP3357909B2 (en) * 1999-03-10 2002-12-16 奈良先端科学技術大学院大学長 How to increase productivity of higher plants
WO2001036444A1 (en) * 1999-11-17 2001-05-25 Mendel Biotechnology, Inc. Plant developmental genes
CA2839066A1 (en) 1999-12-16 2001-06-21 Monsanto Technology Llc Hybrid promoter for use in plants
US7306909B2 (en) * 2000-10-30 2007-12-11 Monsanto Technology Llc Canola event pv-bngt04(rt73) and compositions and methods for detection thereof
US7939715B2 (en) 2000-11-16 2011-05-10 Mendel Biotechnology, Inc. Plants with improved yield and stress tolerance
US8426678B2 (en) 2002-09-18 2013-04-23 Mendel Biotechnology, Inc. Polynucleotides and polypeptides in plants
JP2005130770A (en) * 2003-10-30 2005-05-26 Ajinomoto Co Inc Potato increased in starch level obtained per plant body and method for creating the same
BRPI0514003A (en) * 2004-07-31 2008-05-20 Metanomics Gmbh methods for preparing a non-human organism, preparing a vector, a transgenic plant, a plant cell, a plant tissue, a useful animal's cell, a useful animal or a transgenic microorganism, and fine chemicals, and for identifying a gene product, polynucleotide , vector, host cell, antibody, antisense nucleic acid, non-human organism, transgenic microorganism, seed, tuber or propagation material of a plant, biomass of a microorganism, plant cell, plant cell organelle, plant tissue, plant or part thereof , and, uses of a polypeptide, and nucleic acid molecule
AU2006314188B2 (en) * 2005-11-16 2014-01-16 Pro Natura Gesellschaft Fur Gesunde Ernahrung Mbh Agent for use in the case of fructose intolerance
US8097770B2 (en) * 2006-02-09 2012-01-17 Japan Science And Technology Plant having improved growth ability and disease resistance and method for production thereof
CN102268430B (en) * 2010-06-02 2013-05-15 中国科学院大连化学物理研究所 Fructose-1,6-diphosphate aldolase promoter and application thereof, construct and vector
WO2017053433A1 (en) 2015-09-21 2017-03-30 Modern Meadow, Inc. Fiber reinforced tissue composites
ES2806990T3 (en) 2016-02-15 2021-02-19 Modern Meadow Inc Procedure to manufacture a biofabricated material containing collagen fibrils
CN105973851B (en) * 2016-06-12 2018-10-23 中国科学院地球化学研究所 A kind of bathmic method of quantitative determination plant photosynthesis
CA3008850A1 (en) 2017-06-29 2018-12-29 Modern Meadow, Inc. Yeast strains and methods for producing collagen
AU2018253595A1 (en) 2017-11-13 2019-05-30 Modern Meadow, Inc. Biofabricated leather articles having zonal properties
MX2021008462A (en) 2019-01-17 2021-08-19 Modern Meadow Inc Layered collagen materials and methods of making the same.
CN112094851B (en) * 2020-09-25 2022-01-25 扬州大学 Paeonia ostii PoCAB151 gene, expression vector, and preparation method and application thereof
CN112094859B (en) * 2020-09-25 2022-01-25 扬州大学 Paeonia ostii PoFBA gene, expression vector, preparation method and application thereof
CN113528497B (en) * 2021-07-22 2022-06-24 中国林业科学研究院林业研究所 Use of FBA8 gene or FBA8 protein for preparing reagent with transphosphorylation activity and/or proteolytic activity
CN113528567B (en) * 2021-07-22 2022-05-03 中国林业科学研究院林业研究所 Use of FBA8 protein or protein derived therefrom for regulating vascular bundle division and/or rachis cross-sectional area in plants
CN114920938B (en) * 2022-05-29 2023-11-10 深圳绿天琪生物医药有限公司 Preparation and application of arginine coupled plant source glycan and polypeptide synthetic copolymer

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE212670T1 (en) * 1990-06-18 2002-02-15 Monsanto Technology Llc INCREASED STARCH CONTENT IN PLANTS
DE19502053A1 (en) * 1995-01-13 1996-07-18 Inst Genbiologische Forschung Processes and DNA molecules to increase the rate of photosynthesis in plants, as well as plant cells and plants with an increased rate of photosynthesis
US5716837A (en) * 1995-02-10 1998-02-10 Monsanto Company Expression of sucrose phosphorylase in plants

Also Published As

Publication number Publication date
BR9810171A (en) 2001-11-06
NZ501781A (en) 2001-07-27
CA2294525A1 (en) 1998-12-23
TR199903157T2 (en) 2000-06-21
NO996218L (en) 2000-02-14
CN1267333A (en) 2000-09-20
AU735383B2 (en) 2001-07-05
HUP0004228A3 (en) 2002-11-28
HUP0004228A2 (en) 2001-03-28
ZA985261B (en) 1999-01-07
JP2001520522A (en) 2001-10-30
EA200000029A1 (en) 2000-08-28
IL133532A0 (en) 2001-04-30
AU8144598A (en) 1999-01-04
EP1002114A1 (en) 2000-05-24
NO996218D0 (en) 1999-12-15
PL337581A1 (en) 2000-08-28
WO1998058069A1 (en) 1998-12-23

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