BR9712532A - Method to increase the level of expression of a transgene population of monocotyledged cells, plants and seeds - Google Patents

Method to increase the level of expression of a transgene population of monocotyledged cells, plants and seeds

Info

Publication number
BR9712532A
BR9712532A BR9712532-6A BR9712532A BR9712532A BR 9712532 A BR9712532 A BR 9712532A BR 9712532 A BR9712532 A BR 9712532A BR 9712532 A BR9712532 A BR 9712532A
Authority
BR
Brazil
Prior art keywords
population
expression
plants
transgene
seeds
Prior art date
Application number
BR9712532-6A
Other languages
Portuguese (pt)
Inventor
Joan Tellefsen Odell
Enno Krebbers
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BR9712532A publication Critical patent/BR9712532A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/822—Reducing position variability, e.g. by the use of scaffold attachment region/matrix attachment region (SAR/MAR); Use of SAR/MAR to regulate gene expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)

Abstract

<B>"MéTODO PARA AUMENTAR O NìVEL DE EXPRESSãO DE UM TRANSGENE, POPULAçãO DE CéLULAS MONOCOTILEDÈNEAS, PLANTAS E SEMENTES"<D>. A presente invenção refere-se a um método de aumentar a expressão de transgenes em uma população de plantas monocotiledóneas, o qual envolve o emprego de uma construção de DNA que compreende, entre outras, pelo menos uma região de ligação do tipo plataforma do local de gene de lisozima de galinha.<B> "METHOD FOR INCREASING THE LEVEL OF EXPRESSION OF A TRANSGENE, POPULATION OF MONOCHTILE CELLS, PLANTS AND SEEDS" <D>. The present invention relates to a method of increasing the expression of transgenes in a population of monocotyledonous plants, which involves the use of a DNA construct that comprises, among others, at least one platform-type binding region of the site of chicken lysozyme gene.

BR9712532-6A 1996-10-17 1997-10-01 Method to increase the level of expression of a transgene population of monocotyledged cells, plants and seeds BR9712532A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2816596P 1996-10-17 1996-10-17
PCT/US1997/017709 WO1998016650A1 (en) 1996-10-17 1997-10-01 Enhanced transgene expression in a population of monocot cells employing scaffold attachment regions

Publications (1)

Publication Number Publication Date
BR9712532A true BR9712532A (en) 1999-10-19

Family

ID=21841936

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9712532-6A BR9712532A (en) 1996-10-17 1997-10-01 Method to increase the level of expression of a transgene population of monocotyledged cells, plants and seeds

Country Status (12)

Country Link
EP (1) EP0931155A1 (en)
JP (1) JP2000504943A (en)
KR (1) KR20000049209A (en)
AR (1) AR013853A1 (en)
AU (1) AU4893397A (en)
BR (1) BR9712532A (en)
CA (1) CA2263891A1 (en)
HU (1) HUP0000064A3 (en)
IL (1) IL129268A0 (en)
PL (1) PL332938A1 (en)
WO (1) WO1998016650A1 (en)
ZA (1) ZA979226B (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2292842A1 (en) * 1997-06-03 1998-12-10 North Carolina State University Method for reducing expression variability of transgenes in plants cells
AU3476399A (en) * 1998-07-31 2000-02-21 Mycogen Plant Science, Inc. Improved plant transformation process by scaffold attachment regions (sar)
CN1127570C (en) * 1998-11-20 2003-11-12 中国科学院上海生物化学研究所 Application of castor silkworm cell nucleus and skeleton combination elements
DE19857654A1 (en) 1998-12-14 2000-06-15 Max Planck Gesellschaft Influencing the flowering behavior of plants by expression of sucrose-splitting proteins
US6608242B1 (en) * 2000-05-25 2003-08-19 E. I. Du Pont De Nemours And Company Production of silk-like proteins in plants
AU2002225729A8 (en) 2000-11-14 2009-07-30 Du Pont Modification of a plant disease resistance gene specificity and method for engineering altered specificity
EP1409669B1 (en) 2001-03-23 2009-10-21 E.I. Du Pont De Nemours And Company Root specific, stimulant inducible promoter and its use
AU2003244401A1 (en) 2002-02-04 2003-09-02 Isis Innovation Limited Pyrrolidinyl peptide nucleic acids
CN100480261C (en) * 2003-02-26 2009-04-22 中国科学院上海生命科学研究院 Nuclear stromatin combination sequence and application thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7705215B1 (en) * 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
DE69331908T2 (en) * 1992-10-05 2002-12-12 Univ North Carolina State METHOD FOR INCREASING EXPRESSION AND REDUCING EXPRESSION VARIABILITY OF FOREIGN GENES IN PLANT CELLS
WO1994024293A1 (en) * 1993-04-19 1994-10-27 Sandoz Ltd. Genetic stabilizing elements

Also Published As

Publication number Publication date
IL129268A0 (en) 2000-02-17
KR20000049209A (en) 2000-07-25
WO1998016650A1 (en) 1998-04-23
JP2000504943A (en) 2000-04-25
CA2263891A1 (en) 1998-04-23
PL332938A1 (en) 1999-10-25
AR013853A1 (en) 2001-01-31
EP0931155A1 (en) 1999-07-28
AU4893397A (en) 1998-05-11
ZA979226B (en) 1999-04-15
HUP0000064A2 (en) 2000-05-28
HUP0000064A3 (en) 2002-02-28

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Legal Events

Date Code Title Description
FB34 Technical and formal requirements: requirement - article 34 of industrial property law
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal of application maintained [chapter 11.20 patent gazette]