BR112023024629A2 - Um método para detecção e quantificação simultânea ou independente de listeria monocytogenes, salmonella spp. e escherichia coli e polinucleotídeo - Google Patents
Um método para detecção e quantificação simultânea ou independente de listeria monocytogenes, salmonella spp. e escherichia coli e polinucleotídeoInfo
- Publication number
- BR112023024629A2 BR112023024629A2 BR112023024629A BR112023024629A BR112023024629A2 BR 112023024629 A2 BR112023024629 A2 BR 112023024629A2 BR 112023024629 A BR112023024629 A BR 112023024629A BR 112023024629 A BR112023024629 A BR 112023024629A BR 112023024629 A2 BR112023024629 A2 BR 112023024629A2
- Authority
- BR
- Brazil
- Prior art keywords
- quantification
- escherichia coli
- simultaneous
- listeria monocytogenes
- salmonella spp
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 6
- 238000011002 quantification Methods 0.000 title abstract 5
- 241000588724 Escherichia coli Species 0.000 title abstract 3
- 241000186779 Listeria monocytogenes Species 0.000 title abstract 3
- 241000607142 Salmonella Species 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title abstract 3
- 102000040430 polynucleotide Human genes 0.000 title abstract 2
- 108091033319 polynucleotide Proteins 0.000 title abstract 2
- 239000002157 polynucleotide Substances 0.000 title abstract 2
- 235000013305 food Nutrition 0.000 abstract 3
- 244000052769 pathogen Species 0.000 abstract 2
- 241000251468 Actinopterygii Species 0.000 abstract 1
- 108010079723 Shiga Toxin Proteins 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 abstract 1
- 235000019688 fish Nutrition 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 235000013372 meat Nutrition 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
método para detecção e quantificação simultânea ou independente de listeria monocytogenes, salmonella spp. e escherichia coli e polinucleotídeo. a invenção refere-se a um método para detecção e quantificação simultâneas de listeria monocytogenes, salmonella spp. e escherichia coli produtora de toxina shiga (stec), a partir de qualquer tipo de amostra relacionada à produção de alimentos, incluindo matrizes complexas de alimentos, como peixe, carne ou frutas, ou matrizes simples, como água ou superfícies de contato com alimentos. o método da invenção permite a quantificação específica dos patógenos mencionados acima simultaneamente, graças à especificidade dos primers desenhados para a reação de qpcr. ela também é altamente confiável, porque o método inclui um sistema para controlar adequadamente a quantificação de patógenos dentro da matriz. esse sistema compreende a inoculação de amostras com uma concentração conhecida de um microrganismo transformado (hospedeiro) que carrega uma sequência quimérica, atuando como um hospedeiro de controle interno para todo o processo.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/IB2021/054614 WO2022248917A1 (en) | 2021-05-26 | 2021-05-26 | Method for simultaneous detection and quantification of listeria monocytogenes, salmonella spp., and shiga toxin-producing escherichia coli (stec) |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112023024629A2 true BR112023024629A2 (pt) | 2024-02-20 |
Family
ID=84228454
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112023024629A BR112023024629A2 (pt) | 2021-05-26 | 2021-05-26 | Um método para detecção e quantificação simultânea ou independente de listeria monocytogenes, salmonella spp. e escherichia coli e polinucleotídeo |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP4347614A1 (pt) |
| JP (1) | JP2024520068A (pt) |
| KR (1) | KR20240033215A (pt) |
| BR (1) | BR112023024629A2 (pt) |
| CA (1) | CA3218648A1 (pt) |
| WO (1) | WO2022248917A1 (pt) |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090119022A1 (en) * | 1998-09-25 | 2009-05-07 | Timberlake William E | Emericella Nidulans Genome Sequence On Computer Readable Medium and Uses Thereof |
| US7214786B2 (en) * | 2000-12-14 | 2007-05-08 | Kovalic David K | Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement |
| US20070020640A1 (en) * | 2005-07-21 | 2007-01-25 | Mccloskey Megan L | Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis |
| JP2011188855A (ja) * | 2010-03-11 | 2011-09-29 | Samsung Techwin Co Ltd | 切断可能なキメラプローブを使用したサルモネラのリアルタイム検出方法 |
| CN101928773B (zh) * | 2010-05-14 | 2012-08-29 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | 采用荧光定量pcr技术检测致病菌的寡核苷酸引物组合及其检测致病菌的方法和用途 |
| CN102676647B (zh) * | 2012-02-13 | 2013-08-28 | 东北农业大学 | 检测单增李斯特菌和金黄色葡萄球菌的试剂盒及其应用 |
| US11965216B2 (en) * | 2015-04-07 | 2024-04-23 | Polyskope Labs | Detection of one or more pathogens |
| US20190183130A1 (en) * | 2016-07-18 | 2019-06-20 | Arcis Biotechnology Holdings Limited | Biofumigant |
-
2021
- 2021-05-26 EP EP21942863.8A patent/EP4347614A1/en active Pending
- 2021-05-26 CA CA3218648A patent/CA3218648A1/en active Pending
- 2021-05-26 BR BR112023024629A patent/BR112023024629A2/pt not_active Application Discontinuation
- 2021-05-26 KR KR1020237044554A patent/KR20240033215A/ko unknown
- 2021-05-26 WO PCT/IB2021/054614 patent/WO2022248917A1/en active Application Filing
- 2021-05-26 JP JP2023573155A patent/JP2024520068A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CA3218648A1 (en) | 2022-12-01 |
| JP2024520068A (ja) | 2024-05-21 |
| KR20240033215A (ko) | 2024-03-12 |
| EP4347614A1 (en) | 2024-04-10 |
| WO2022248917A1 (en) | 2022-12-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B11A | Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing | ||
| B11Y | Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette] |