BR112022024625A2 - Construtos de ácido nucleico para a fabricação de proteína - Google Patents

Construtos de ácido nucleico para a fabricação de proteína

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Publication number
BR112022024625A2
BR112022024625A2 BR112022024625A BR112022024625A BR112022024625A2 BR 112022024625 A2 BR112022024625 A2 BR 112022024625A2 BR 112022024625 A BR112022024625 A BR 112022024625A BR 112022024625 A BR112022024625 A BR 112022024625A BR 112022024625 A2 BR112022024625 A2 BR 112022024625A2
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BR
Brazil
Prior art keywords
nucleic acid
acid constructs
protein manufacturing
protein
cell lines
Prior art date
Application number
BR112022024625A
Other languages
English (en)
Inventor
T Bleck Gregory
H Kravitz Rachel
A Hall Chad
Original Assignee
Catalent Pharma Solutions Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Catalent Pharma Solutions Llc filed Critical Catalent Pharma Solutions Llc
Publication of BR112022024625A2 publication Critical patent/BR112022024625A2/pt

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/10041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/10043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/48—Vector systems having a special element relevant for transcription regulating transport or export of RNA, e.g. RRE, PRE, WPRE, CTE
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

A presente invenção se refere a construtos de ácido nucleico e seu uso para desenvolver linhagem de células hospedeiras para a produção de uma proteína de interesse e, em particular, a construtos de ácido nucleico que permitem a seleção melhorada para desenvolver linhagens de células de alta produção.
BR112022024625A 2020-06-02 2021-06-02 Construtos de ácido nucleico para a fabricação de proteína BR112022024625A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063033516P 2020-06-02 2020-06-02
US202063033514P 2020-06-02 2020-06-02
PCT/US2021/035404 WO2021247672A1 (en) 2020-06-02 2021-06-02 Nucleic acid constructs for protein manufacture

Publications (1)

Publication Number Publication Date
BR112022024625A2 true BR112022024625A2 (pt) 2023-02-23

Family

ID=78830554

Family Applications (2)

Application Number Title Priority Date Filing Date
BR112022024625A BR112022024625A2 (pt) 2020-06-02 2021-06-02 Construtos de ácido nucleico para a fabricação de proteína
BR112022024644A BR112022024644A2 (pt) 2020-06-02 2021-06-02 Linhagens celulares com múltiplas ancoragens para inserção de gene

Family Applications After (1)

Application Number Title Priority Date Filing Date
BR112022024644A BR112022024644A2 (pt) 2020-06-02 2021-06-02 Linhagens celulares com múltiplas ancoragens para inserção de gene

Country Status (10)

Country Link
US (2) US20230227858A1 (pt)
EP (2) EP4158042A1 (pt)
JP (2) JP2023528475A (pt)
KR (2) KR20230019156A (pt)
CN (2) CN116134136A (pt)
AU (2) AU2021283272A1 (pt)
BR (2) BR112022024625A2 (pt)
CA (2) CA3180705A1 (pt)
MX (2) MX2022015208A (pt)
WO (2) WO2021247672A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3119799A1 (en) * 2018-12-04 2020-06-11 Catalent Pharma Solutions, Llc Vectors for protein manufacture

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6897066B1 (en) * 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
EP3269802B1 (en) * 2011-09-30 2019-10-23 Bluebird Bio, Inc. Compounds for improved viral transduction
CN111647627A (zh) * 2014-04-28 2020-09-11 重组股份有限公司 多重基因编辑
EP3443085B1 (en) * 2016-04-14 2022-09-14 BOCO Silicon Valley, Inc. Genome editing of human neural stem cells using nucleases
CA3119799A1 (en) * 2018-12-04 2020-06-11 Catalent Pharma Solutions, Llc Vectors for protein manufacture
US20240026350A1 (en) * 2020-04-07 2024-01-25 Io Biosciences, Inc. Nucleic acid constructs comprising gene editing multi-sites

Also Published As

Publication number Publication date
JP2023529376A (ja) 2023-07-10
BR112022024644A2 (pt) 2023-02-23
WO2021247672A1 (en) 2021-12-09
US20230227858A1 (en) 2023-07-20
WO2021247671A3 (en) 2022-01-06
JP2023528475A (ja) 2023-07-04
EP4158041A4 (en) 2024-09-25
CA3180217A1 (en) 2021-12-09
US20230212590A1 (en) 2023-07-06
CA3180705A1 (en) 2021-12-09
EP4158041A2 (en) 2023-04-05
CN115803440A (zh) 2023-03-14
AU2021284288A1 (en) 2023-01-05
MX2022015202A (es) 2023-02-15
MX2022015208A (es) 2023-02-15
KR20230021676A (ko) 2023-02-14
EP4158042A1 (en) 2023-04-05
CN116134136A (zh) 2023-05-16
KR20230019156A (ko) 2023-02-07
AU2021283272A1 (en) 2023-01-19
WO2021247671A2 (en) 2021-12-09

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