BR112022016801A2 - Edição eficiente do genoma em células mieloides primárias - Google Patents

Edição eficiente do genoma em células mieloides primárias

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Publication number
BR112022016801A2
BR112022016801A2 BR112022016801A BR112022016801A BR112022016801A2 BR 112022016801 A2 BR112022016801 A2 BR 112022016801A2 BR 112022016801 A BR112022016801 A BR 112022016801A BR 112022016801 A BR112022016801 A BR 112022016801A BR 112022016801 A2 BR112022016801 A2 BR 112022016801A2
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Brazil
Prior art keywords
myeloid cells
genome editing
primary myeloid
efficient genome
efficient
Prior art date
Application number
BR112022016801A
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English (en)
Inventor
Krishna Murthy Aditya
Oh Jaehak
John Bohlen Christopher
Crane Freund Emily
Joseph Haley Benjamin
Lock Jaclyn
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of BR112022016801A2 publication Critical patent/BR112022016801A2/pt

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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Abstract

EDIÇÃO EFICIENTE DO GENOMA EM CÉLULAS MIELOIDES PRIMÁRIAS. São fornecidos aqui, inter alia, composições, métodos e sistemas para manipulação genética eficiente de células mielodes sem o uso de vetores virais. Além disso, são fornecidas estratégias para ruptura de genes em células mieloides primárias (por exemplo, de origem humana e murina) usando distribuição baseada em eletroporação de proteínas Cas/ribonucleares (RNPs). Os métodos fornecidos neste documento, incluindo modalidades dos mesmos, podem fornecer nocaute genético próximo ao nível populacional de alvos únicos e múltiplos em uma variedade de tipos de células sem seleção ou enriquecimento. A aptidão celular e a resposta a estímulos imunológicos podem não ser afetadas pelo processo de edição de genes.
BR112022016801A 2020-02-28 2021-02-24 Edição eficiente do genoma em células mieloides primárias BR112022016801A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202062983568P 2020-02-28 2020-02-28
US202063010476P 2020-04-15 2020-04-15
PCT/US2021/019488 WO2021173729A2 (en) 2020-02-28 2021-02-24 Efficient genome editing in primary myeloid cells

Publications (1)

Publication Number Publication Date
BR112022016801A2 true BR112022016801A2 (pt) 2022-10-11

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ID=77490516

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Application Number Title Priority Date Filing Date
BR112022016801A BR112022016801A2 (pt) 2020-02-28 2021-02-24 Edição eficiente do genoma em células mieloides primárias

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US (1) US20230295665A1 (pt)
EP (1) EP4110903A4 (pt)
JP (1) JP2023516968A (pt)
KR (1) KR20220147614A (pt)
CN (1) CN115298305A (pt)
AU (1) AU2021227663A1 (pt)
BR (1) BR112022016801A2 (pt)
CA (1) CA3173287A1 (pt)
IL (1) IL295871A (pt)
TW (1) TW202146649A (pt)
WO (1) WO2021173729A2 (pt)

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US20240294943A1 (en) * 2023-03-03 2024-09-05 Charles River Laboratories, Inc. Method for improving genetic engineering of adoptive cell therapies

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018081476A2 (en) * 2016-10-27 2018-05-03 Intima Bioscience, Inc. Viral methods of t cell therapy
WO2018111947A1 (en) * 2016-12-12 2018-06-21 Integrated Dna Technologies, Inc. Genome editing enhancement
US20210254068A1 (en) * 2018-06-19 2021-08-19 Regents Of The University Of Minnesota Genome engineering primary monocytes

Also Published As

Publication number Publication date
EP4110903A2 (en) 2023-01-04
TW202146649A (zh) 2021-12-16
JP2023516968A (ja) 2023-04-21
CN115298305A (zh) 2022-11-04
IL295871A (en) 2022-10-01
AU2021227663A1 (en) 2022-09-22
US20230295665A1 (en) 2023-09-21
EP4110903A4 (en) 2024-04-17
WO2021173729A3 (en) 2021-10-21
KR20220147614A (ko) 2022-11-03
CA3173287A1 (en) 2021-09-02
WO2021173729A2 (en) 2021-09-02

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