BR112022005069A2 - Vetores grandes e métodos para produção de alto rendimento - Google Patents
Vetores grandes e métodos para produção de alto rendimentoInfo
- Publication number
- BR112022005069A2 BR112022005069A2 BR112022005069A BR112022005069A BR112022005069A2 BR 112022005069 A2 BR112022005069 A2 BR 112022005069A2 BR 112022005069 A BR112022005069 A BR 112022005069A BR 112022005069 A BR112022005069 A BR 112022005069A BR 112022005069 A2 BR112022005069 A2 BR 112022005069A2
- Authority
- BR
- Brazil
- Prior art keywords
- bacterial cells
- methods
- culture medium
- vector
- replication
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/101—Plasmid DNA for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2820/00—Vectors comprising a special origin of replication system
- C12N2820/002—Vectors comprising a special origin of replication system inducible or controllable
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2820/00—Vectors comprising a special origin of replication system
- C12N2820/55—Vectors comprising a special origin of replication system from bacteria
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
vetores grandes e métodos para produção de alto rendimento. são fornecidos no presente documento métodos para a produção de um vetor com um tamanho de pelo menos 16 kb de células bacterianas que compreendem as etapas consecutivas da) obter células bacterianas que compreendem um vetor com um tamanho de pelo menos 16 kb, que compreende uma origem induzível de replicação, b) inocular meio de cultura com as células bacterianas que compreendem o vetor, c) cultivar as células bacterianas no meio de cultura, d) adicionar um ou mais indutores da dita origem induzível de replicação ao meio de cultura quando a cultura bacteriana tiver alcançado uma densidade óptica a 600 nm (od600) de pelo menos 20, e) cultivar adicionalmente as células bacterianas no meio de cultura, f) opcionalmente separar as células bacterianas do meio de cultura, e g) recuperar o plasmídeo das células bacterianas. também são fornecidos no presente documento vetores com um tamanho de pelo menos 16 kb que compreendem uma origem induzível de replicação para uso em tais métodos.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP19199473 | 2019-09-25 | ||
PCT/EP2020/076864 WO2021058722A1 (en) | 2019-09-25 | 2020-09-25 | Large vectors and methods for high-yield production |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112022005069A2 true BR112022005069A2 (pt) | 2022-06-21 |
Family
ID=68109104
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112022005069A BR112022005069A2 (pt) | 2019-09-25 | 2020-09-25 | Vetores grandes e métodos para produção de alto rendimento |
Country Status (11)
Country | Link |
---|---|
US (1) | US20230183718A1 (pt) |
EP (1) | EP4034662A1 (pt) |
JP (1) | JP2022554070A (pt) |
KR (1) | KR20220066384A (pt) |
CN (1) | CN114450408A (pt) |
AU (1) | AU2020353279A1 (pt) |
BR (1) | BR112022005069A2 (pt) |
CA (1) | CA3149999A1 (pt) |
IL (1) | IL291507A (pt) |
MX (1) | MX2022003627A (pt) |
WO (1) | WO2021058722A1 (pt) |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8407498D0 (en) | 1984-03-22 | 1984-05-02 | Biogen Nv | High copy number expression vectors |
GB201307528D0 (en) | 2013-04-26 | 2013-06-12 | Univ Leuven Kath | Bacterial artificial chromosomes |
-
2020
- 2020-09-25 BR BR112022005069A patent/BR112022005069A2/pt unknown
- 2020-09-25 CA CA3149999A patent/CA3149999A1/en active Pending
- 2020-09-25 AU AU2020353279A patent/AU2020353279A1/en active Pending
- 2020-09-25 CN CN202080066336.1A patent/CN114450408A/zh active Pending
- 2020-09-25 KR KR1020227013617A patent/KR20220066384A/ko unknown
- 2020-09-25 WO PCT/EP2020/076864 patent/WO2021058722A1/en active Application Filing
- 2020-09-25 JP JP2022519402A patent/JP2022554070A/ja active Pending
- 2020-09-25 MX MX2022003627A patent/MX2022003627A/es unknown
- 2020-09-25 US US17/636,175 patent/US20230183718A1/en active Pending
- 2020-09-25 EP EP20772189.5A patent/EP4034662A1/en active Pending
-
2022
- 2022-03-20 IL IL291507A patent/IL291507A/en unknown
Also Published As
Publication number | Publication date |
---|---|
CN114450408A (zh) | 2022-05-06 |
JP2022554070A (ja) | 2022-12-28 |
IL291507A (en) | 2022-05-01 |
WO2021058722A1 (en) | 2021-04-01 |
CA3149999A1 (en) | 2021-04-01 |
EP4034662A1 (en) | 2022-08-03 |
MX2022003627A (es) | 2022-07-21 |
KR20220066384A (ko) | 2022-05-24 |
US20230183718A1 (en) | 2023-06-15 |
AU2020353279A1 (en) | 2022-02-24 |
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