BR112021019433A2 - Método para produzir estruturas de polímeros tridimensionais de célula em fluxo, método para sequenciamento e célula de fluxo - Google Patents

Método para produzir estruturas de polímeros tridimensionais de célula em fluxo, método para sequenciamento e célula de fluxo

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Publication number
BR112021019433A2
BR112021019433A2 BR112021019433A BR112021019433A BR112021019433A2 BR 112021019433 A2 BR112021019433 A2 BR 112021019433A2 BR 112021019433 A BR112021019433 A BR 112021019433A BR 112021019433 A BR112021019433 A BR 112021019433A BR 112021019433 A2 BR112021019433 A2 BR 112021019433A2
Authority
BR
Brazil
Prior art keywords
flow cell
precursor solution
producing
dimensional
polymer precursor
Prior art date
Application number
BR112021019433A
Other languages
English (en)
Inventor
Elisabet Rosas-Canyelles
Hayden Black
Mathieu Lessard-Viger
Max Zimmerley
Sean Ramirez
Kumar Khurana Tarun
Yir-Shyuan Wu
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from NL2024527A external-priority patent/NL2024527B1/en
Priority claimed from NL2024528A external-priority patent/NL2024528B1/en
Priority claimed from NL2024596A external-priority patent/NL2024596B1/en
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of BR112021019433A2 publication Critical patent/BR112021019433A2/pt

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  • Immunology (AREA)
  • Polymers & Plastics (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Fluid Mechanics (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract

método para produzir estruturas de polímeros tridimensionais de célula em fluxo, método para sequenciamento e célula de fluxo. um método para produzir estruturas de polímero tridimensionais de célula em fluxo inclui carregar uma solução precursora de polímero em uma célula de fluxo. a solução precursora de polímero inclui um monômero, um reticulador e um fotoiniciador. a célula de fluxo inclui ao menos um canal para receber a solução precursora de polímero. o ao menos um canal tem uma superfície interna superior e uma superfície interna inferior. o método inclui adicionalmente iluminar a solução precursora de polímero através de uma fotomáscara dotada de um padrão usando uma luz em um comprimento de onda suficiente para ativar o fotoiniciador. a ativação do fotoiniciador polimeriza ao menos parte da solução precursora de polímero sob as aberturas na fotomáscara dotada de um padrão e forma estruturas de polímero tridimensionais que se estendem desde a superfície interna superior até a superfície interna inferior do ao menos um canal.
BR112021019433A 2019-11-27 2020-11-25 Método para produzir estruturas de polímeros tridimensionais de célula em fluxo, método para sequenciamento e célula de fluxo BR112021019433A2 (pt)

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US201962941197P 2019-11-27 2019-11-27
US201962941242P 2019-11-27 2019-11-27
US201962941215P 2019-11-27 2019-11-27
NL2024527A NL2024527B1 (en) 2019-11-27 2019-12-20 On-flow cell three-dimensional polymer structures
NL2024528A NL2024528B1 (en) 2019-11-27 2019-12-20 On-flow cell three-dimensional polymer structures having functionalized surfaces
NL2024596A NL2024596B1 (en) 2019-11-27 2019-12-31 On-flow cell three-dimensional sequencing matrices
PCT/US2020/062163 WO2021108499A1 (en) 2019-11-27 2020-11-25 On-flow cell three-dimensional polymer structures

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