BR112017021854A2 - ?método para bloquear mirna? - Google Patents

?método para bloquear mirna?

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Publication number
BR112017021854A2
BR112017021854A2 BR112017021854A BR112017021854A BR112017021854A2 BR 112017021854 A2 BR112017021854 A2 BR 112017021854A2 BR 112017021854 A BR112017021854 A BR 112017021854A BR 112017021854 A BR112017021854 A BR 112017021854A BR 112017021854 A2 BR112017021854 A2 BR 112017021854A2
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BR
Brazil
Prior art keywords
nucleic acid
blocking nucleic
mirna
region
blocking
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BR112017021854A
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English (en)
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BR112017021854B1 (pt
Inventor
s roberts Brian
M Myers Richard
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Hudsonalpha Inst For Biotechnology
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Publication date
Application filed by Hudsonalpha Inst For Biotechnology filed Critical Hudsonalpha Inst For Biotechnology
Publication of BR112017021854A2 publication Critical patent/BR112017021854A2/pt
Publication of BR112017021854B1 publication Critical patent/BR112017021854B1/pt

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
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    • C12N2320/00—Applications; Uses
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    • C12N2330/00—Production
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    • C12N2330/31—Libraries, arrays
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/207—Modifications characterised by siRNA, miRNA
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/163—Reactions characterised by the reaction format or use of a specific feature the purpose or use of blocking probe

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

ácido nucleico de bloqueio para o uso em reduzir a abundância de um micro-rna (mirna) não-alvo em uma biblioteca de mirna é fornecido, incluindo: uma região complementar de fita única em uma da extremidade 5? do ácido nucleico de bloqueio ou da extremidade 3? do ácido nucleico de bloqueio, que associa com uma região de ligação em uma primeira extremidade do mirna indesejado; uma região formadora de hairpin loop ou outra região de fita dupla adjacente à região de complementaridade, em que todas as extermidades terminais do ácido nucleico de bloqueio exceto um não estão disponíveis para participar em reações de ligase. métodos e kits para usar o ácido nucleico de bloqueio também são fornecidos.
BR112017021854-2A 2015-04-10 2016-04-10 MÉTODOS PARA PREVENIR UM MICRO-RNA (miRNA) INDESEJADO DE PARTICIPAR EM REAÇÕES EM CADEIA DA POLIMERASE DA TRANSCRIÇÃO REVERSA (RT-PCR) BR112017021854B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562146015P 2015-04-10 2015-04-10
US62/146,015 2015-04-10
PCT/US2016/026846 WO2016164866A1 (en) 2015-04-10 2016-04-10 METHOD FOR BLOCKING miRNA

Publications (2)

Publication Number Publication Date
BR112017021854A2 true BR112017021854A2 (pt) 2018-07-10
BR112017021854B1 BR112017021854B1 (pt) 2024-03-26

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Also Published As

Publication number Publication date
EP3280805B1 (en) 2020-12-16
IL254972A0 (en) 2017-12-31
JP6791875B2 (ja) 2020-11-25
AU2016246820A1 (en) 2017-11-30
IL254972B (en) 2021-05-31
US20200024652A1 (en) 2020-01-23
JP2018513685A (ja) 2018-05-31
EP3280805A4 (en) 2019-03-13
US10480023B2 (en) 2019-11-19
CA2982369A1 (en) 2016-10-13
MX2017013022A (es) 2018-03-15
US20180073066A1 (en) 2018-03-15
US11781176B2 (en) 2023-10-10
EP3280805A1 (en) 2018-02-14
WO2016164866A1 (en) 2016-10-13
AU2016246820B2 (en) 2021-08-12

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 10/04/2016, OBSERVADAS AS CONDICOES LEGAIS