BR112016023818A2 - método para a diferenciação das células estaminais pluripotentes em cardiomiócitos, cardiomiócitos, biobanco de cardiomiócitos, utilização dos cardiomiócitos composição terapêutica e métodos e utilizações - Google Patents

método para a diferenciação das células estaminais pluripotentes em cardiomiócitos, cardiomiócitos, biobanco de cardiomiócitos, utilização dos cardiomiócitos composição terapêutica e métodos e utilizações

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Publication number
BR112016023818A2
BR112016023818A2 BR112016023818A BR112016023818A BR112016023818A2 BR 112016023818 A2 BR112016023818 A2 BR 112016023818A2 BR 112016023818 A BR112016023818 A BR 112016023818A BR 112016023818 A BR112016023818 A BR 112016023818A BR 112016023818 A2 BR112016023818 A2 BR 112016023818A2
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BR
Brazil
Prior art keywords
cardiomyocytes
stem cells
pluripotent stem
biobank
cardiomyocyte
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BR112016023818A
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English (en)
Inventor
Burcin Mark
SCHLICHT Sonja
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Hoffmann La Roche
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Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of BR112016023818A2 publication Critical patent/BR112016023818A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Cardiology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Rheumatology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Developmental Biology & Embryology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Vascular Medicine (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

a presente invenção se refere a um método para a diferenciação das células estaminais pluripotentes (pscs) em cardiomiócitos. além disso, a presente invenção se refere a um método para a diferenciação das células estaminais embrionárias humanas (hescs) e células estaminais pluripotentes induzidas (ipscs) em cardiomiócitos definidas com base em etapas de ligação de induções do meio quimicamente definido.
BR112016023818A 2014-05-06 2015-05-04 método para a diferenciação das células estaminais pluripotentes em cardiomiócitos, cardiomiócitos, biobanco de cardiomiócitos, utilização dos cardiomiócitos composição terapêutica e métodos e utilizações BR112016023818A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14167201 2014-05-06
PCT/EP2015/059745 WO2015169762A1 (en) 2014-05-06 2015-05-04 Method for differentiation of pluripotent stem cells into cardiomyocytes

Publications (1)

Publication Number Publication Date
BR112016023818A2 true BR112016023818A2 (pt) 2017-08-15

Family

ID=50679891

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016023818A BR112016023818A2 (pt) 2014-05-06 2015-05-04 método para a diferenciação das células estaminais pluripotentes em cardiomiócitos, cardiomiócitos, biobanco de cardiomiócitos, utilização dos cardiomiócitos composição terapêutica e métodos e utilizações

Country Status (10)

Country Link
US (1) US10155927B2 (pt)
EP (1) EP3140395B1 (pt)
JP (1) JP6744223B2 (pt)
KR (1) KR20160147800A (pt)
CN (1) CN106661553B (pt)
BR (1) BR112016023818A2 (pt)
CA (1) CA2943035A1 (pt)
MX (1) MX2016014008A (pt)
RU (1) RU2016146567A (pt)
WO (1) WO2015169762A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107475185A (zh) * 2016-06-07 2017-12-15 杨旅军 一种用于培养人间充质干细胞的无动物源成分培养基
CN110506109B (zh) * 2017-06-05 2024-03-12 泰尔茂株式会社 细胞培养物的制造方法
JP2022535141A (ja) 2019-06-06 2022-08-04 プレジデント アンド フェローズ オブ ハーバード カレッジ 心筋細胞及び組成物ならびにそれらを生成する方法
EP4110900A4 (en) * 2020-02-28 2024-04-24 Purdue Research Foundation GENERATING AORTA-GONAD-MESONEPHROS-LIKE HEMATOPOETIC CELLS FROM HUMAN PLURIPOTENT STEM CELLS UNDER A DEFINED STATE
CN113249310B (zh) * 2021-05-17 2022-10-11 湖北大学 一种拓展性多能干细胞诱导分化为心肌细胞的方法及应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7727762B2 (en) * 2003-10-03 2010-06-01 Keiichi Fukuda Method of inducing the differentiation of stem cells into myocardial cells
US20080254003A1 (en) * 2004-12-22 2008-10-16 Robert Passier Differentiation of Human Embryonic Stem Cells and Cardiomyocytes and Cardiomyocyte Progenitors Derived Therefrom
US9062289B2 (en) * 2005-06-22 2015-06-23 Asterias Biotherapeutics, Inc. Differentiation of primate pluripotent stem cells to cardiomyocyte-lineage cells
UA103918C2 (en) * 2009-03-02 2013-12-10 Айерем Элелси N-(hetero)aryl, 2-(hetero)aryl-substituted acetamides for use as wnt signaling modulators
EP4166652A1 (en) * 2009-11-12 2023-04-19 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
CN103209968B (zh) * 2010-08-26 2015-08-05 国立大学法人京都大学 多能干细胞心肌细胞分化促进剂
WO2012168167A1 (en) * 2011-06-09 2012-12-13 F. Hoffmann-La Roche Ag Method for differentiation of pluripotent stem cells into vascular bed cells
WO2013056072A1 (en) * 2011-10-13 2013-04-18 Wisconsin Alumni Research Foundation Generation of cardiomyocytes from human pluripotent stem cells
JP6494515B2 (ja) * 2012-10-19 2019-04-03 エージェンシー フォー サイエンス， テクノロジー アンド リサーチ 幹細胞を１又は２以上の細胞系列に分化させる方法

Also Published As

Publication number Publication date
CA2943035A1 (en) 2015-11-12
RU2016146567A3 (pt) 2018-12-10
KR20160147800A (ko) 2016-12-23
JP6744223B2 (ja) 2020-08-19
WO2015169762A1 (en) 2015-11-12
CN106661553B (zh) 2020-09-01
MX2016014008A (es) 2017-01-11
EP3140395A1 (en) 2017-03-15
RU2016146567A (ru) 2018-06-08
CN106661553A (zh) 2017-05-10
US20150353893A1 (en) 2015-12-10
US10155927B2 (en) 2018-12-18
JP2017518739A (ja) 2017-07-13
EP3140395B1 (en) 2020-01-22

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