BR112015011190A2 - Método de modificação dirigida e célula de alga geneticamente modificada - Google Patents
Método de modificação dirigida e célula de alga geneticamente modificadaInfo
- Publication number
- BR112015011190A2 BR112015011190A2 BR112015011190A BR112015011190A BR112015011190A2 BR 112015011190 A2 BR112015011190 A2 BR 112015011190A2 BR 112015011190 A BR112015011190 A BR 112015011190A BR 112015011190 A BR112015011190 A BR 112015011190A BR 112015011190 A2 BR112015011190 A2 BR 112015011190A2
- Authority
- BR
- Brazil
- Prior art keywords
- algae cell
- genetically modified
- genetic material
- directed modification
- modification method
- Prior art date
Links
- 241000195493 Cryptophyta Species 0.000 title abstract 3
- 238000002715 modification method Methods 0.000 title 1
- 102000004533 Endonucleases Human genes 0.000 abstract 3
- 108010042407 Endonucleases Proteins 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 101710163270 Nuclease Proteins 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
- C12N1/125—Unicellular algae isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/89—Algae ; Processes using algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07009—UTP-glucose-1-phosphate uridylyltransferase (2.7.7.9), i.e. UDP-glucose-pyrophosphorylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/11—Exodeoxyribonucleases producing 5'-phosphomonoesters (3.1.11)
- C12Y301/11002—Exodeoxyribonuclease III (3.1.11.2)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261727444P | 2012-11-16 | 2012-11-16 | |
| US201361839165P | 2013-06-25 | 2013-06-25 | |
| PCT/IB2013/002974 WO2014076571A2 (en) | 2012-11-16 | 2013-11-18 | Method for targeted modification of algae genomes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112015011190A2 true BR112015011190A2 (pt) | 2017-10-03 |
Family
ID=50231458
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112015011190A BR112015011190A2 (pt) | 2012-11-16 | 2013-11-18 | Método de modificação dirigida e célula de alga geneticamente modificada |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20160272980A1 (enExample) |
| EP (1) | EP2920310A2 (enExample) |
| CN (1) | CN105121649A (enExample) |
| AU (1) | AU2013346504A1 (enExample) |
| BR (1) | BR112015011190A2 (enExample) |
| IN (1) | IN2015DN04148A (enExample) |
| WO (1) | WO2014076571A2 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2014301147B2 (en) * | 2013-06-25 | 2020-07-30 | Cellectis | Modified diatoms for biofuel production |
| WO2016168756A1 (en) | 2015-04-15 | 2016-10-20 | Synthetic Genomics, Inc. | Algal chloroplastic srp54 mutants |
| CN105567681B (zh) * | 2015-12-31 | 2018-08-31 | 广州赛哲生物科技股份有限公司 | 一种基于高通量基因测序无创活检病毒的方法及标签接头 |
| CN117683836A (zh) * | 2017-05-09 | 2024-03-12 | 辛利斯生物制药有限责任公司 | 用于修饰微囊藻毒素和节球藻毒素的方法 |
| WO2019113463A1 (en) | 2017-12-08 | 2019-06-13 | Synthetic Genomics, Inc. | Improving algal lipid productivity via genetic modification of a tpr domain containing protein |
| EP3732189A4 (en) | 2017-12-29 | 2022-01-19 | Synthetic Genomics, Inc. | GENETIC MODULATION OF PHOTOSYNTHETIC ORGANISMS FOR IMPROVED GROWTH |
| US11352618B2 (en) | 2018-03-23 | 2022-06-07 | Sean Raspet | Modified organisms for improved flavor and aroma |
| WO2019200318A1 (en) * | 2018-04-13 | 2019-10-17 | Sean Raspet | Modified organisms for improved flavor and aroma |
| CN112813091A (zh) * | 2021-02-03 | 2021-05-18 | 山东农业大学 | Nat1基因作为筛选标记在卵菌遗传转化中的应用 |
| CA3250471A1 (en) | 2022-04-14 | 2023-10-19 | Institute Of Agricultural Resources And Regional Planning Of The Chinese Academy Of Agricultural Sciences | MICROALGAE MODIFIED FOR BETTER PHOSPHATE ABSORPTION INVOLVING THE OVEREXPRESSION OF PSR1 AND POSSIBLY THE UNDEREXPRESSION OF PTC1 |
| EP4638699A1 (en) | 2022-12-19 | 2025-10-29 | Hutanbio Ltd. | Lipid producing marine microalga |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| FR2646438B1 (fr) | 1989-03-20 | 2007-11-02 | Pasteur Institut | Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration |
| US5516670A (en) | 1991-09-30 | 1996-05-14 | Kuehnle; Adelheid R. | Magnetophoretic particle delivery method and apparatus for the treatment of cells |
| US6027900A (en) * | 1996-04-12 | 2000-02-22 | Carnegie Institution Of Washington | Methods and tools for transformation of eukaryotic algae |
| US6706394B2 (en) | 2000-05-02 | 2004-03-16 | Manfred R. Kuehnle | Method and apparatus for manufacture of magnetizable microparticles |
| EP1485475B2 (en) | 2002-03-15 | 2017-09-20 | Cellectis | Hybrid and single chain meganucleases and use thereof |
| WO2009095742A1 (en) | 2008-01-31 | 2009-08-06 | Cellectis | New i-crei derived single-chain meganuclease and uses thereof |
| WO2006097854A1 (en) | 2005-03-15 | 2006-09-21 | Cellectis | Heterodimeric meganucleases and use thereof |
| WO2008093152A1 (en) | 2007-02-01 | 2008-08-07 | Cellectis | Obligate heterodimer meganucleases and uses thereof |
| CN102762726A (zh) * | 2009-11-27 | 2012-10-31 | 巴斯夫植物科学有限公司 | 嵌合内切核酸酶及其用途 |
| BR112012012588B1 (pt) | 2009-11-27 | 2019-03-26 | Basf Plant Science Company Gmbh | Endonuclease, método para recombinação homóloga de polinucleotídeos e método para mutação direcionada de polinucleotídeos |
| CN103025344B (zh) | 2010-05-17 | 2016-06-29 | 桑格摩生物科学股份有限公司 | 新型dna-结合蛋白及其用途 |
| US8608877B2 (en) * | 2010-07-27 | 2013-12-17 | General Electric Company | Nickel alloy and articles |
| SG187680A1 (en) * | 2010-08-02 | 2013-03-28 | Cellectis | Method for targeted genomic events in algae |
| EP3603662B1 (en) * | 2011-02-28 | 2022-01-19 | Seattle Children's Research Institute | Coupling endonucleases with end-processing enzymes drive high efficiency gene disruption |
| KR101982360B1 (ko) | 2011-04-05 | 2019-05-24 | 셀렉티스 | 콤팩트 tale-뉴클레아제의 발생 방법 및 이의 용도 |
-
2013
- 2013-11-18 CN CN201380070530.7A patent/CN105121649A/zh active Pending
- 2013-11-18 WO PCT/IB2013/002974 patent/WO2014076571A2/en not_active Ceased
- 2013-11-18 BR BR112015011190A patent/BR112015011190A2/pt not_active Application Discontinuation
- 2013-11-18 IN IN4148DEN2015 patent/IN2015DN04148A/en unknown
- 2013-11-18 EP EP13834373.6A patent/EP2920310A2/en not_active Withdrawn
- 2013-11-18 US US14/442,323 patent/US20160272980A1/en not_active Abandoned
- 2013-11-18 AU AU2013346504A patent/AU2013346504A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CN105121649A (zh) | 2015-12-02 |
| AU2013346504A1 (en) | 2015-06-18 |
| WO2014076571A8 (en) | 2014-07-10 |
| WO2014076571A9 (en) | 2015-04-02 |
| EP2920310A2 (en) | 2015-09-23 |
| IN2015DN04148A (enExample) | 2015-10-16 |
| WO2014076571A2 (en) | 2014-05-22 |
| WO2014076571A3 (en) | 2014-11-06 |
| US20160272980A1 (en) | 2016-09-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06I | Publication of requirement cancelled [chapter 6.9 patent gazette] |
Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA. |
|
| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B11B | Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements | ||
| B350 | Update of information on the portal [chapter 15.35 patent gazette] |