BR112014002121A2 - modelo de câncer de mama de animal knock-in não humano pik3ca h1047r - Google Patents
modelo de câncer de mama de animal knock-in não humano pik3ca h1047rInfo
- Publication number
- BR112014002121A2 BR112014002121A2 BR112014002121A BR112014002121A BR112014002121A2 BR 112014002121 A2 BR112014002121 A2 BR 112014002121A2 BR 112014002121 A BR112014002121 A BR 112014002121A BR 112014002121 A BR112014002121 A BR 112014002121A BR 112014002121 A2 BR112014002121 A2 BR 112014002121A2
- Authority
- BR
- Brazil
- Prior art keywords
- breast cancer
- pik3ca
- knock
- model
- cancer model
- Prior art date
Links
- 208000026310 Breast neoplasm Diseases 0.000 title abstract 8
- 206010006187 Breast cancer Diseases 0.000 title abstract 6
- 102200085789 rs121913279 Human genes 0.000 title abstract 4
- 241001465754 Metazoa Species 0.000 title abstract 2
- 206010069754 Acquired gene mutation Diseases 0.000 abstract 2
- 101150080074 TP53 gene Proteins 0.000 abstract 2
- 230000004075 alteration Effects 0.000 abstract 2
- 238000003745 diagnosis Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 230000035772 mutation Effects 0.000 abstract 2
- 230000037439 somatic mutation Effects 0.000 abstract 2
- 230000002269 spontaneous effect Effects 0.000 abstract 2
- 230000005740 tumor formation Effects 0.000 abstract 2
- 208000028647 spindle cell neoplasm Diseases 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
- A61K49/0008—Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/20—Animal model comprising regulated expression system
- A01K2217/206—Animal model comprising tissue-specific expression system, e.g. tissue specific expression of transgene, of Cre recombinase
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
- A01K2267/0331—Animal model for proliferative diseases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
- C12N2015/8527—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic for producing animal models, e.g. for tests or diseases
- C12N2015/8572—Animal models for proliferative diseases, e.g. comprising an oncogene
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Diabetes (AREA)
- Rheumatology (AREA)
- Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Endocrinology (AREA)
- Urology & Nephrology (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Plant Pathology (AREA)
Abstract
abstract of the disclosure the invention concerns the development of a pik3ca h1047r knock-in non-human animal breast cancer model, and its use for identification of a spontaneous loss-of-function tp53 mutation involved in spindle cell tumor formation. the invention further concerns the identification of additional somatic mutations and copy number aberrations in the breast tumors using this model, and methods and means for the diagnosis and treatment of breast cancer. ___________________________________________________________________________________ tradução do resumo resumo patente de invenção: "modelo de câncer de mama de animal knock-in não humano pik3ca h1047r". a invenção envolve o desenvolvimento de um modelo de câncer de mama animal não humano knock-in pik3ca h1047r e seu uso para identificação de uma mutação tp53 de perda de função espontânea envolvida na formação de tumor de célula fusiforme. a invenção ainda envolve a identificação de mutações somáticas adicionais e aberrações de número de cópia nos tumores de mama usando este modelo e métodos e meios para o diagnóstico e tratamento de câncer de mama.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161574161P | 2011-07-28 | 2011-07-28 | |
PCT/US2012/000336 WO2013015833A2 (en) | 2011-07-28 | 2012-07-26 | Pik3ca h1047r knock-in non-human animal breast cancer model |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112014002121A2 true BR112014002121A2 (pt) | 2017-02-21 |
Family
ID=46690680
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112014002121A BR112014002121A2 (pt) | 2011-07-28 | 2012-07-26 | modelo de câncer de mama de animal knock-in não humano pik3ca h1047r |
Country Status (10)
Country | Link |
---|---|
US (1) | US20140298493A1 (pt) |
EP (1) | EP2736325B1 (pt) |
JP (1) | JP2014529298A (pt) |
KR (1) | KR20140050689A (pt) |
CN (1) | CN104010494B (pt) |
BR (1) | BR112014002121A2 (pt) |
CA (1) | CA2843222A1 (pt) |
MX (1) | MX2014001063A (pt) |
RU (1) | RU2014107713A (pt) |
WO (1) | WO2013015833A2 (pt) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6078383B2 (ja) * | 2013-03-07 | 2017-02-08 | 学校法人順正学園 | トランスジェニック非ヒト哺乳動物 |
EP3842554B1 (en) * | 2014-05-09 | 2022-12-14 | Memorial Sloan Kettering Cancer Center | Biomarkers for response to pi3k inhibitors |
CA2985894A1 (en) * | 2015-05-15 | 2016-11-24 | Memorial Sloan-Kettering Cancer Center | Use of phosphoinositide 3-kinase inhibitors for treatment of vascular malformations |
JP2018531606A (ja) * | 2015-10-30 | 2018-11-01 | ザ ジャクソン ラボラトリーThe Jackson Laboratory | 腫瘍分析に関する組成物及び方法 |
RU2608960C1 (ru) * | 2016-03-18 | 2017-01-27 | Федеральное государственное бюджетное учреждение "Российский онкологический научный центр имени Н.Н. Блохина" Министерства здравоохранения Российской Федерации (ФГБУ "РОНЦ им. Н.Н. Блохина" Минздрава России) | Клеточная линия crov Cel муцинозного рака яичников человека, предназначенная для разработки лечения таргетными препаратами |
CN107828786A (zh) * | 2017-10-24 | 2018-03-23 | 昆明理工大学 | 靶向敲除PIK3CA基因的sgRNA及应用 |
BR112021015353A2 (pt) | 2019-02-06 | 2021-10-05 | Venthera, Inc. | Inibidores de fosfoinositídeo 3-cinase tópicos |
KR102191341B1 (ko) | 2019-03-15 | 2020-12-16 | 국립암센터 | 유방암 동물모델의 제조방법 및 이의 용도 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
PL3296407T3 (pl) * | 2004-03-02 | 2019-11-29 | Univ Johns Hopkins | Mutacje genu pik3ca w nowotworach ludzkich |
EP2041139B1 (en) | 2006-04-26 | 2011-11-09 | F. Hoffmann-La Roche AG | Pharmaceutical compounds |
EP1978106A1 (en) * | 2007-04-07 | 2008-10-08 | Universitätsklinikum Hamburg-Eppendorf | Detection of ESR1 amplification in endometrium cancer and ovary cancer |
JP2010523670A (ja) * | 2007-04-10 | 2010-07-15 | エクセリクシス, インク. | Pi3kアルファのピリドピリミジノン阻害剤を使用した治療方法 |
MX2010006039A (es) * | 2007-12-03 | 2010-06-23 | Enzon Pharmaceuticals Inc | Compuestos antagonistas del acido ribonucleico para la modulacion de la expresion de la fosfoinositida-3-cinasa catalitica. |
EP2279188B1 (en) * | 2008-05-30 | 2015-01-28 | Genentech, Inc. | Purine pi3k inhibitor compounds and methods of use |
CN101445832B (zh) * | 2008-12-23 | 2011-09-14 | 广州益善生物技术有限公司 | Pik3ca基因突变的检测探针、液相芯片及其检测方法 |
WO2011031861A1 (en) * | 2009-09-09 | 2011-03-17 | Quintiles Transnational Corp. | Methods for predicting responsiveness of a disease or disorder to a receptor tyrosine kinase inhibitor by analysis of mutations in pik3ca |
WO2011060380A1 (en) * | 2009-11-14 | 2011-05-19 | The Regents Of The University Of California | Pik3ca mutation status and sash1 expression predicts synergy between lapatinib and an akt inhibitor in her2 positive breast cancer |
EP2510121A1 (en) * | 2009-12-11 | 2012-10-17 | Wyeth LLC | Phosphatidylinositol-3-kinase pathway biomarkers |
EP2524231B1 (en) * | 2010-01-13 | 2017-06-28 | Wyeth LLC | A CUT-POINT IN PTEN PROTEIN EXPRESSION THAT ACCURATELY IDENTIFIES TUMORS AND IS PREDICTIVE OF DRUG RESPONSE TO A PAN-ErbB INHIBITOR |
-
2012
- 2012-07-26 RU RU2014107713/10A patent/RU2014107713A/ru not_active Application Discontinuation
- 2012-07-26 KR KR1020147004811A patent/KR20140050689A/ko not_active Application Discontinuation
- 2012-07-26 CN CN201280046858.0A patent/CN104010494B/zh active Active
- 2012-07-26 US US14/233,976 patent/US20140298493A1/en not_active Abandoned
- 2012-07-26 EP EP12748281.8A patent/EP2736325B1/en active Active
- 2012-07-26 WO PCT/US2012/000336 patent/WO2013015833A2/en active Application Filing
- 2012-07-26 JP JP2014522813A patent/JP2014529298A/ja active Pending
- 2012-07-26 CA CA2843222A patent/CA2843222A1/en not_active Abandoned
- 2012-07-26 BR BR112014002121A patent/BR112014002121A2/pt not_active IP Right Cessation
- 2012-07-26 MX MX2014001063A patent/MX2014001063A/es unknown
Also Published As
Publication number | Publication date |
---|---|
MX2014001063A (es) | 2014-09-01 |
CA2843222A1 (en) | 2013-01-31 |
WO2013015833A2 (en) | 2013-01-31 |
CN104010494B (zh) | 2017-07-04 |
EP2736325A2 (en) | 2014-06-04 |
WO2013015833A3 (en) | 2013-09-26 |
RU2014107713A (ru) | 2015-09-10 |
EP2736325B1 (en) | 2018-10-10 |
KR20140050689A (ko) | 2014-04-29 |
JP2014529298A (ja) | 2014-11-06 |
US20140298493A1 (en) | 2014-10-02 |
CN104010494A (zh) | 2014-08-27 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] | ||
B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |