BR0311594A - human cell assay to determine the effect of sample compounds on col2 enhancer expression - Google Patents

human cell assay to determine the effect of sample compounds on col2 enhancer expression

Info

Publication number
BR0311594A
BR0311594A BRPI0311594-1A BR0311594A BR0311594A BR 0311594 A BR0311594 A BR 0311594A BR 0311594 A BR0311594 A BR 0311594A BR 0311594 A BR0311594 A BR 0311594A
Authority
BR
Brazil
Prior art keywords
col2
effect
sample compounds
activity
human cell
Prior art date
Application number
BRPI0311594-1A
Other languages
Portuguese (pt)
Inventor
Leonard Buckbinder
Jean Frances Schaefer
Original Assignee
Pfizer Prod Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Prod Inc filed Critical Pfizer Prod Inc
Publication of BR0311594A publication Critical patent/BR0311594A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"ENSAIO DE CéLULAS HUMANAS PARA DETERMINAR O EFEITO DE COMPOSTOS DE AMOSTRA NA EXPRESSãO DE INTENSIFICADOR DE COL2". A presente invenção refere-se à atividade (crescimento/diferenciação/produção de matriz extracelular) de condrócitos humanos e ao efeito do fator de transcrição Sox9 e do intensificador de Col2 como medida de tal atividade. Refere-se também a uma célula quimérica e a um ensaio utilizando tal célula útil na previsão do efeito de compostos de amostra na atividade de condrócitos medida através da expressão/atividade de Sox9 e Col2."HUMAN CELL TEST TO DETERMINE EFFECT OF SAMPLE COMPOUNDS ON COL2 INTENSIFIER EXPRESSION". The present invention relates to the activity (growth / differentiation / extracellular matrix production) of human chondrocytes and the effect of the transcription factor Sox9 and Col2 enhancer as a measure of such activity. It also refers to a chimeric cell and an assay using such a cell useful in predicting the effect of sample compounds on chondrocyte activity measured by the expression / activity of Sox9 and Col2.

BRPI0311594-1A 2002-06-04 2003-05-26 human cell assay to determine the effect of sample compounds on col2 enhancer expression BR0311594A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38615602P 2002-06-04 2002-06-04
PCT/IB2003/002256 WO2003102130A2 (en) 2002-06-04 2003-05-26 A human cell assay to determine effect of sample compounds on col2 enhancer expression

Publications (1)

Publication Number Publication Date
BR0311594A true BR0311594A (en) 2007-04-27

Family

ID=29712234

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0311594-1A BR0311594A (en) 2002-06-04 2003-05-26 human cell assay to determine the effect of sample compounds on col2 enhancer expression

Country Status (7)

Country Link
EP (1) EP1576098A2 (en)
JP (1) JP2006500915A (en)
AU (1) AU2003233009A1 (en)
BR (1) BR0311594A (en)
CA (1) CA2488006A1 (en)
MX (1) MXPA04012042A (en)
WO (1) WO2003102130A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8791084B2 (en) 2006-10-27 2014-07-29 Robarts Research Institute Inhibition of SOX9 function in the treatment of proteoglycan-associated pathophysiological conditions
KR102084227B1 (en) * 2018-09-05 2020-03-03 (주)루젠에스씨아이 Human transformed chondrocyte cell line mediated drug screening system for cartilage disease remedy

Also Published As

Publication number Publication date
MXPA04012042A (en) 2005-03-07
AU2003233009A8 (en) 2003-12-19
WO2003102130A3 (en) 2006-06-22
AU2003233009A1 (en) 2003-12-19
EP1576098A2 (en) 2005-09-21
WO2003102130A2 (en) 2003-12-11
CA2488006A1 (en) 2003-12-11
JP2006500915A (en) 2006-01-12

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