BR0215958A - Methods of modulating the production of a sulfur-containing compound by a microorganism and of methionine production, transgenic expression cassette, vector, and transgenic host cell - Google Patents

Methods of modulating the production of a sulfur-containing compound by a microorganism and of methionine production, transgenic expression cassette, vector, and transgenic host cell

Info

Publication number
BR0215958A
BR0215958A BR0215958-9A BR0215958A BR0215958A BR 0215958 A BR0215958 A BR 0215958A BR 0215958 A BR0215958 A BR 0215958A BR 0215958 A BR0215958 A BR 0215958A
Authority
BR
Brazil
Prior art keywords
methionine
production
methods
transgenic
nucleic acid
Prior art date
Application number
BR0215958-9A
Other languages
Portuguese (pt)
Other versions
BRPI0215958B1 (en
Inventor
Markus Pompejus
Burkhard Kr Ger
Hartwig Sch Der
Oskar Zelder
Gregor Haberhauer
Stefan Haefner
Corinna Klopprogge
Original Assignee
Basf Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag filed Critical Basf Ag
Publication of BR0215958A publication Critical patent/BR0215958A/en
Publication of BRPI0215958B1 publication Critical patent/BRPI0215958B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium

Abstract

"MéTODOS DE MODULAçãO DA PRODUçãO DE UM COMPOSTO CONTENDO ENXOFRE POR UM MICROORGANISMO E DE PRODUçãO DE METIONINA, CASSETE DE EXPRESSãO TRANSGêNICO, VETOR, E, CéLULA HOSPEDEIRA TRANSGêNICA". Moléculas de ácido nucleico, chamadas de moléculas de ácido nucleico MR, que codificam novas proteínas MR de Corynebacterium glutamicum são descritas as quais estão envolvidas na biossíntese de um composto químico fino, por exemplo na biossintese de metionina. A invenção também proporciona moléculas de ácido nucleico de anti-senso, vetores e cassetes de expressão transgênicos contendo moléculas de ácido nucleico MR e células hospedeiras para dentro das quais os vetores ou cassetes de expressão têm sido introduzidos. A invenção ainda adicionalmente proporciona métodos de produção de metionina a partir de microorganismos, por exemplo C. glutamicum, que envolve a cultura de microorganismos recombinantes que superexpressam ou subexpressam pelo menos uma molécula MR da invenção sob condições tais que metionina é produzida. Também são apresentados métodos de produção de um composto químico fino, por exemplo metionina, que envolve a cultura de microorganismos recombinantes possuindo genes MR selecionados deletados ou mutados sob condições tais que o composto químico fino, por exemplo metionina, é produzido."METHODS OF MODULATING PRODUCTION OF A COMPOUND CONTAINING SULFUR BY A MICROORGANISM AND METHYMINE PRODUCTION, TRANSGENIC EXPRESSION CASSETTE, AND, TRANSGENIC HOST CELL". Nucleic acid molecules, called MR nucleic acid molecules, which encode new Corynebacterium glutamicum MR proteins are described which are involved in the biosynthesis of a fine chemical compound, for example in methionine biosynthesis. The invention also provides antisense nucleic acid molecules, vectors and transgenic expression cassettes containing MR nucleic acid molecules and host cells into which expression vectors or cassettes have been introduced. The invention further provides methods of producing methionine from microorganisms, for example C. glutamicum, which involves culturing recombinant microorganisms that overexpress or underexpress at least one MR molecule of the invention under conditions such that methionine is produced. Also disclosed are methods of producing a fine chemical compound, for example methionine, which involves culturing recombinant microorganisms having deleted or mutated selected MR genes under conditions such that the fine chemical compound, for example methionine, is produced.

BRPI0215958A 2002-11-29 2002-11-29 methionine production methods BRPI0215958B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2002/013504 WO2004050694A1 (en) 2002-11-29 2002-11-29 Methods for the production of methionine

Publications (2)

Publication Number Publication Date
BR0215958A true BR0215958A (en) 2005-09-13
BRPI0215958B1 BRPI0215958B1 (en) 2016-11-08

Family

ID=32405659

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0215958A BRPI0215958B1 (en) 2002-11-29 2002-11-29 methionine production methods

Country Status (6)

Country Link
EP (1) EP1567549A1 (en)
JP (1) JP2006521083A (en)
CN (1) CN1694899B (en)
AU (1) AU2002368410A1 (en)
BR (1) BRPI0215958B1 (en)
WO (1) WO2004050694A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004035052A1 (en) * 2004-07-20 2006-02-16 Basf Ag Microorganisms for the production of sulfur-containing compounds
WO2008101857A2 (en) 2007-02-19 2008-08-28 Evonik Degussa Gmbh Coryneform bacteria with formate-thf-synthetase and/or glycine cleavage activity
AR066984A1 (en) 2007-06-15 2009-09-23 Novartis Ag INHIBITION OF THE EXPRESSION OF THE ALFA SUBUNITY OF THE SODIUM EPITELIAL CHANNEL (ENAC) THROUGH ARNI (INTERFERENCE RNA)
DE102008001874A1 (en) * 2008-05-20 2009-11-26 Evonik Degussa Gmbh Process for the preparation of L-amino acids
WO2015028674A1 (en) 2013-08-30 2015-03-05 Metabolic Explorer Microorganism for methionine production with improved methionine synthase activity and methionine efflux
KR102254631B1 (en) * 2021-01-15 2021-05-21 씨제이제일제당 주식회사 Novel Peptide methionine sulfoxide reductase variant and a method for producing IMP using the same
KR102281359B1 (en) * 2021-01-26 2021-07-22 씨제이제일제당 (주) Novel protein variant and a method for producing L-valine using the same

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4623825B2 (en) * 1999-12-16 2011-02-02 協和発酵バイオ株式会社 Novel polynucleotide
DE10109690A1 (en) * 2000-09-02 2002-03-14 Degussa New nucleotide sequences encoding the metY gene
DE10126164A1 (en) * 2001-05-30 2002-12-05 Degussa Nucleotide sequences coding for the metD gene

Also Published As

Publication number Publication date
AU2002368410A1 (en) 2004-06-23
EP1567549A1 (en) 2005-08-31
CN1694899A (en) 2005-11-09
BRPI0215958B1 (en) 2016-11-08
WO2004050694A1 (en) 2004-06-17
JP2006521083A (en) 2006-09-21
CN1694899B (en) 2011-07-06

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Legal Events

Date Code Title Description
B25D Requested change of name of applicant approved

Owner name: BASF SE (DE)

Free format text: ALTERADO DE: BASF AKTIENGESELLSCHAFT

B25A Requested transfer of rights approved

Owner name: EVONIK DEGUSSA GMBH (DE)

Free format text: TRANSFERIDO DE: BASF SE

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B15K Others concerning applications: alteration of classification

Free format text: AS CLASSIFICACOES ANTERIORES ERAM: C07K 14/34 , C12P 13/12 , C12N 15/31 , C12R 1/15 , C12R 1/13

Ipc: C12N 15/31 (2006.01), C12P 13/12 (2006.01), C07K 1

B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B09X Republication of the decision to grant [chapter 9.1.3 patent gazette]

Free format text: REF. A RPI NO 2387 DE 04/10/2016.

B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 08/11/2016, OBSERVADAS AS CONDICOES LEGAIS.

B25D Requested change of name of applicant approved