BR0208402A - Métodos diagnósticos para detecção de uma ou mais mutações de citocromo b em fungos - Google Patents
Métodos diagnósticos para detecção de uma ou mais mutações de citocromo b em fungosInfo
- Publication number
- BR0208402A BR0208402A BR0208402-3A BR0208402A BR0208402A BR 0208402 A BR0208402 A BR 0208402A BR 0208402 A BR0208402 A BR 0208402A BR 0208402 A BR0208402 A BR 0208402A
- Authority
- BR
- Brazil
- Prior art keywords
- cytochrome
- diagnostic methods
- mutations
- detecting
- technique
- Prior art date
Links
- 230000035772 mutation Effects 0.000 title abstract 4
- 238000002405 diagnostic procedure Methods 0.000 title abstract 3
- 102100025287 Cytochrome b Human genes 0.000 title abstract 2
- 108010075028 Cytochromes b Proteins 0.000 title abstract 2
- 241000233866 Fungi Species 0.000 title 1
- 238000000034 method Methods 0.000 abstract 3
- 108700028369 Alleles Proteins 0.000 abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 2
- 230000002538 fungal effect Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 108010052832 Cytochromes Proteins 0.000 abstract 1
- 102000018832 Cytochromes Human genes 0.000 abstract 1
- 238000009007 Diagnostic Kit Methods 0.000 abstract 1
- 206010034133 Pathogen resistance Diseases 0.000 abstract 1
- 101900111531 Saccharomyces cerevisiae Cytochrome b Proteins 0.000 abstract 1
- 229930182692 Strobilurin Natural products 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
"MéTODOS DIAGNóSTICOS PARA DETECçãO DE UMA OU MAIS MUTAçõES DE CITOCROMO E EM FUNGOS". Essa invenção se relaciona a métodos diagnósticos para detecção para uma ou mais mutações de citocromo b em fungos na posição que corresponde ao resíduo 129 do citocromo b de Saccharomyces cerevisiae que leva à resistência a análogos ou compostos de estrobilurina no mesmo grupo de resistência cruzada usando qualquer (ou uma) técnica de detecção de polimorfismo num único nucleotídeo, preferentemente usando técnica de amplificação com especificidade de alelo tal como o sistema de amplificação de mutação refratária (ARMS) ou preferentemente usando uma técnica de marcador de hibridização seletiva para alelo tal como "Molecular Beacons" ou TaqMan. A invenção também se relaciona a oligonucleotídeos com especificidade de mutação para uso no método e a kits diagnósticos contendo esses oligonucleotídeos.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0108227A GB0108227D0 (en) | 2001-04-02 | 2001-04-02 | Methods |
GB0122697A GB0122697D0 (en) | 2001-09-20 | 2001-09-20 | Methods |
PCT/GB2002/001411 WO2002081742A2 (en) | 2001-04-02 | 2002-03-25 | Method for the detection of cytochrome b mutations in fungi |
Publications (1)
Publication Number | Publication Date |
---|---|
BR0208402A true BR0208402A (pt) | 2004-03-30 |
Family
ID=26245924
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR0208402-3A BR0208402A (pt) | 2001-04-02 | 2002-03-25 | Métodos diagnósticos para detecção de uma ou mais mutações de citocromo b em fungos |
Country Status (9)
Country | Link |
---|---|
US (1) | US20050014144A1 (pt) |
EP (1) | EP1421207A2 (pt) |
JP (1) | JP2004533230A (pt) |
KR (1) | KR20030087030A (pt) |
CN (1) | CN1496410A (pt) |
BR (1) | BR0208402A (pt) |
CA (1) | CA2436278A1 (pt) |
RU (1) | RU2296162C2 (pt) |
WO (1) | WO2002081742A2 (pt) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050118647A1 (en) * | 2001-12-31 | 2005-06-02 | Yung-Chiang Chung | Microfluidic mixer apparatus and microfluidic reactor apparatus for microfluidic processing |
KR100658178B1 (ko) * | 2005-03-02 | 2006-12-14 | 인제대학교 산학협력단 | 인간 사이토크롬 p450 2b6 유전자의 변이 유전형 분석방법 |
CN101235415B (zh) * | 2007-01-30 | 2010-07-21 | 中山大学达安基因股份有限公司 | 以TaqMan探针定量多聚酶链反应技术检测基因单点突变的方法 |
CA2900859A1 (en) * | 2014-08-18 | 2016-02-18 | Yingfu Li | Compositions and methods for detection of a target in a molecular assay using ph changes |
AR105291A1 (es) * | 2015-07-10 | 2017-09-20 | Bayer Sas | Métodos y conjuntos de elementos para la detección del oídio |
CN107254549A (zh) * | 2017-08-21 | 2017-10-17 | 魏宏泉 | 一种用于基因突变检测的方法 |
CZ309242B6 (cs) * | 2017-10-31 | 2022-06-15 | VÝZKUMNÝ A ŠLECHTITELSKÝ ÚSTAV OVOCNÁŘSKÝ HOLOVOUSY s.r.o | Sada pro detekci mutace G143A způsobující rezistenci ke QoI fungicidům |
CN109266723A (zh) * | 2018-09-25 | 2019-01-25 | 北京协和洛克生物技术有限责任公司 | 稀有突变检测方法、其试剂盒及应用 |
AU2021266044A1 (en) * | 2020-04-28 | 2022-11-24 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution F129L in the mitochondrial cytochrome b protein conferring resistance to Qo inhibitors IV |
EP3903582A1 (en) * | 2020-04-28 | 2021-11-03 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors ii |
JP2023527959A (ja) * | 2020-04-28 | 2023-07-03 | ビーエーエスエフ ソシエタス・ヨーロピア | ミトコンドリアチトクロムbタンパク質中にQo阻害剤IIIに対して耐性を付与するアミノ酸置換F129Lを含む植物病原性菌類を駆除するためのストロビルリン型化合物の使用 |
EP3903581A1 (en) * | 2020-04-28 | 2021-11-03 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors i |
WO2021219386A1 (en) * | 2020-04-28 | 2021-11-04 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors i |
EP3903584A1 (en) * | 2020-04-28 | 2021-11-03 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors iv |
EP3903583A1 (en) * | 2020-04-28 | 2021-11-03 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors iii |
CN115460920A (zh) * | 2020-04-28 | 2022-12-09 | 巴斯夫欧洲公司 | 嗜球果伞素类型化合物防除在线粒体细胞色素b蛋白中含有赋予对Qo抑制剂II的耐受性的氨基酸替代F129L的植物病原性真菌的用途 |
CN112322709B (zh) * | 2020-11-23 | 2022-07-05 | 河北省农林科学院植物保护研究所 | 快速鉴定马铃薯晚疫病菌Cytb基因核苷酸点突变及其对吡唑醚菌酯抗性的方法 |
CA3140203A1 (en) * | 2020-11-24 | 2022-05-24 | Winfield Solutions, Llc | Soil pathogen testing |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5639611A (en) * | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
-
2002
- 2002-03-25 CN CNA028060865A patent/CN1496410A/zh active Pending
- 2002-03-25 JP JP2002579505A patent/JP2004533230A/ja not_active Withdrawn
- 2002-03-25 BR BR0208402-3A patent/BR0208402A/pt not_active IP Right Cessation
- 2002-03-25 EP EP02714333A patent/EP1421207A2/en not_active Withdrawn
- 2002-03-25 CA CA002436278A patent/CA2436278A1/en not_active Abandoned
- 2002-03-25 RU RU2003132073/13A patent/RU2296162C2/ru not_active IP Right Cessation
- 2002-03-25 KR KR10-2003-7012724A patent/KR20030087030A/ko not_active Application Discontinuation
- 2002-03-25 WO PCT/GB2002/001411 patent/WO2002081742A2/en not_active Application Discontinuation
- 2002-03-25 US US10/483,979 patent/US20050014144A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US20050014144A1 (en) | 2005-01-20 |
RU2296162C2 (ru) | 2007-03-27 |
CN1496410A (zh) | 2004-05-12 |
RU2003132073A (ru) | 2005-02-27 |
CA2436278A1 (en) | 2002-10-17 |
WO2002081742A3 (en) | 2003-04-03 |
WO2002081742A2 (en) | 2002-10-17 |
JP2004533230A (ja) | 2004-11-04 |
KR20030087030A (ko) | 2003-11-12 |
EP1421207A2 (en) | 2004-05-26 |
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Legal Events
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B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 6A,7A E 8A ANUIDADES |
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B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2055 DE 25/05/2010. |