BR0017326A - Redução de transmissão de transgenes em plantas - Google Patents

Redução de transmissão de transgenes em plantas

Info

Publication number
BR0017326A
BR0017326A BR0017326-6A BR0017326A BR0017326A BR 0017326 A BR0017326 A BR 0017326A BR 0017326 A BR0017326 A BR 0017326A BR 0017326 A BR0017326 A BR 0017326A
Authority
BR
Brazil
Prior art keywords
plant
promoter
sequences
excision
gene
Prior art date
Application number
BR0017326-6A
Other languages
English (en)
Inventor
Venkatesan Sundaresan
Yan Hong
Original Assignee
Inst Of Molecular Agrobiology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Of Molecular Agrobiology filed Critical Inst Of Molecular Agrobiology
Publication of BR0017326A publication Critical patent/BR0017326A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8265—Transgene containment, e.g. gene dispersal

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"REDUçãO DE TRANSMISSãO DE TRANSGENES EM PLANTAS". A presente invenção refere-se a um método para criar uma planta geneticamente modificada expressando um transgene com uma característica desejável que é mal transmitida, a característica sendo perdida nas gerações seguintes devido à excisão do transgene. O método envolve hibridizar uma primeira planta regenerada de uma célula de planta que foi transfectada com seq³encias de DNA compreendendo um primeiro gene cuja expressão resulta em um fenótipo de planta alterado ligado a um promotor, o cassete de gene e promotor flanqueados sobre cada lado pelas primeiras seq³ências de excisão ligadas a um promotor transitoriamente ativo, o gene e promotor estando separados por uma seq³ência de bloqueio flanqueada sobre cada lado pelas segundas seq³ências de excisão específicas para uma segunda planta regenerada de uma segunda célula de planta que foi transfectada com seq³ências de DNA compreendendo um terceiro gene que codifica uma recombinase específica para as segundas seq³ências de excisão ligadas a um promotor, e desenvolvendo uma planta híbrida da semente híbrida. Alternativamente, uma planta simples pode ser estavelmente transformada com todas as seq³ências acima para realizar o mesmo resultado. As células de planta, tecidos de planta, semente de planta e plantas inteiras contendo as seq³ências de DNA acima são também descritas.
BR0017326-6A 2000-08-25 2000-08-25 Redução de transmissão de transgenes em plantas BR0017326A (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/SG2000/000124 WO2002016624A1 (en) 2000-08-25 2000-08-25 Reduction of transmission of transgenes in plants

Publications (1)

Publication Number Publication Date
BR0017326A true BR0017326A (pt) 2003-06-24

Family

ID=20428851

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0017326-6A BR0017326A (pt) 2000-08-25 2000-08-25 Redução de transmissão de transgenes em plantas

Country Status (7)

Country Link
EP (1) EP1313865A1 (pt)
CN (1) CN1296484C (pt)
AR (1) AR032632A1 (pt)
AU (2) AU7048700A (pt)
BR (1) BR0017326A (pt)
CA (1) CA2419646A1 (pt)
WO (1) WO2002016624A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0113026A (pt) * 2000-07-28 2003-07-15 Univ Connecticut Método para a extração controlada, automática de dna heterólogo de plantas transgênicas e cassetes gênicos de extração de dna para uso no disposto
EP1794305A2 (en) * 2004-09-23 2007-06-13 BASF Plant Science GmbH Recombination cassettes and methods for sequence excision in plants
WO2008043844A1 (en) * 2006-10-13 2008-04-17 Vrije Universiteit Brussel Preparation of transgenic plants
CN103215280B (zh) * 2013-05-08 2014-08-20 山东省农业科学院高新技术研究中心 一种花生spl转录因子基因及其编码蛋白与应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993001283A1 (en) * 1991-07-08 1993-01-21 The United States Of America As Represented By The Secretary Of Agriculture Selection-gene-free transgenic plants
EG23907A (en) * 1994-08-01 2007-12-30 Delta & Pine Land Co Control of plant gene expression
US7135608B1 (en) * 1997-08-28 2006-11-14 The Salk Institute For Biological Studies Site-specific recombination in eukaryotes and constructs useful therefor
US8017829B1 (en) * 1997-09-05 2011-09-13 Purdue Research Foundation Selective expression of genes in plants
ZA989782B (en) * 1997-10-30 1999-05-04 Mogen Int Pre-and postharvest inhibition of remobilisation of storage compounds
US5929301A (en) * 1997-11-18 1999-07-27 Pioneer Hi-Bred International Nucleic acid sequence encoding FLP recombinase

Also Published As

Publication number Publication date
AU7048700A (en) 2002-03-04
CN1296484C (zh) 2007-01-24
CA2419646A1 (en) 2002-02-28
WO2002016624A1 (en) 2002-02-28
AR032632A1 (es) 2003-11-19
EP1313865A1 (en) 2003-05-28
CN1454261A (zh) 2003-11-05
AU2000270487B2 (en) 2006-06-15

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Legal Events

Date Code Title Description
B08F Application fees: dismissal - article 86 of industrial property law

Free format text: REFERENTE A 5A, 6A E 7A ANUIDADES.

B08K Lapse as no evidence of payment of the annual fee has been furnished to inpi (acc. art. 87)

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1929 DE 26/12/2007.