BR0017215A - Flavivirus detection and quantification analysis - Google Patents

Flavivirus detection and quantification analysis

Info

Publication number
BR0017215A
BR0017215A BR0017215-4A BR0017215A BR0017215A BR 0017215 A BR0017215 A BR 0017215A BR 0017215 A BR0017215 A BR 0017215A BR 0017215 A BR0017215 A BR 0017215A
Authority
BR
Brazil
Prior art keywords
analysis
flavivirus
detection
pcr
dengue virus
Prior art date
Application number
BR0017215-4A
Other languages
Portuguese (pt)
Inventor
Huo-Chu Houng
Niranjan Kanesa-Thasan
Original Assignee
Us Army Med Res Mat Command
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/551,161 external-priority patent/US6793488B1/en
Application filed by Us Army Med Res Mat Command filed Critical Us Army Med Res Mat Command
Publication of BR0017215A publication Critical patent/BR0017215A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes

Abstract

"DETECçãO DE FLAVIVìRUS E ANáLISES DE QUANTIFICAçãO". São descritas ondas de DNA fluorescentes específicas e flanqueando pares de iniciadores, com base na seq³ência de informação encontrada na região não-codificadora de 3' terminal conservada de flavivírus, por exemplo, nucleotídeos 10653-10678 de vírus da dengue. Reação em cadeia de polimerase fluorogênica, empregando esse iniciadores e sondas, produzem resultados que permitem a identificação específica de flavivírus. Essa análises podem ser ambas quantitativas e qualitativas. São descritas condições ótima de análise com antecedente zero, a qual permite a detecção de baixos níveis de flavivírus a partir de espécimes clínicas. Especificamente, o vírus da dengue, isolado a partir de diferentes regiões geográficas, pode ser universalmente detectado e identificado pela análise RT-PCR fluorogência descrita. A análise RT-PCR fluorogência descrita prontamente detectou viremia em soros coletados de indivíduos doentes, com dengue."DETECTION OF FLAVIVIRUS AND QUANTIFICATION ANALYSIS". Specific fluorescent DNA waves flanking primer pairs are described, based on the sequence of information found in the conserved flavivirus non-terminal 3 'coding region, for example, dengue virus nucleotides 10653-10678. Fluorogenic polymerase chain reaction, employing these primers and probes, yield results that enable specific identification of flaviviruses. These analyzes can be both quantitative and qualitative. Optimal conditions of zero antecedent analysis are described, which allows the detection of low flavivirus levels from clinical specimens. Specifically, dengue virus, isolated from different geographic regions, can be universally detected and identified by the described fluorogency RT-PCR analysis. The described RT-PCR fluorogence analysis promptly detected viremia in sera collected from dengue sick individuals.

BR0017215-4A 2000-04-14 2000-10-19 Flavivirus detection and quantification analysis BR0017215A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/551,161 US6793488B1 (en) 1999-04-16 2000-04-14 Flavivirus detection and quantification assay
PCT/US2000/028961 WO2001079546A2 (en) 2000-04-14 2000-10-19 Flavivirus detection and quantification assay

Publications (1)

Publication Number Publication Date
BR0017215A true BR0017215A (en) 2004-02-10

Family

ID=24200105

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0017215-4A BR0017215A (en) 2000-04-14 2000-10-19 Flavivirus detection and quantification analysis

Country Status (5)

Country Link
EP (1) EP1276898A1 (en)
AU (1) AU2001212172A1 (en)
BR (1) BR0017215A (en)
CA (1) CA2405960A1 (en)
WO (1) WO2001079546A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7927840B2 (en) 2006-09-11 2011-04-19 Gen Probe Incorporated Method for detecting West Nile Virus nucleic acids in the 3′ non-coding region
CA2501946C (en) * 2002-10-16 2014-12-23 Gen-Probe Incorporated Compositions and methods for detecting west nile virus
AU2008200221B2 (en) * 2002-10-16 2012-04-05 Gen-Probe Incorporated Compositions and methods for detecting west nile virus
WO2013080307A1 (en) * 2011-11-29 2013-06-06 株式会社 東芝 Primer set for amplifying mosquito-borne virus, assay kit for detecting mosquito-borne virus, and detection method using said primer set and said assay kit
CN104342498B (en) * 2013-07-30 2019-04-16 中国疾病预防控制中心病毒病预防控制所 Culex flavivirus real-time fluorescence quantitative RT-PCR detection method and kit
CN104357582B (en) * 2014-10-31 2017-02-15 中国检验检疫科学研究院 Real-time fluorescence RT-PCR detection kit and method of Kunjin virus
CN107893128A (en) * 2017-11-09 2018-04-10 贵州医科大学 The primer pair combination, probe and application of 10 kinds of arboviruses of detection simultaneously
CN110846441B (en) * 2019-12-20 2023-04-11 浙江省淡水水产研究所 Specific primer, probe and rapid detection kit for detecting macrobrachium flaviviridae-1

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5939254A (en) * 1997-04-28 1999-08-17 University Of Massachusetts Methods and reagents for rapid diagnosis of dengue virus infection
US5968732A (en) * 1997-12-31 1999-10-19 Akzo Nobel, N.V. Isothermal transcription based assay for the detection and genotyping of dengue virus

Also Published As

Publication number Publication date
EP1276898A1 (en) 2003-01-22
WO2001079546A3 (en) 2003-06-05
CA2405960A1 (en) 2001-10-25
WO2001079546A2 (en) 2001-10-25
AU2001212172A1 (en) 2001-10-30

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]

Free format text: O DEPOSITANTE DEVERA APRESENTAR A PETICAO DEVIDA PARA O CUMPRIMENTO DESTA EXIGENCIA EM ATE 60 (SESSENTA) DIAS, CONTADOS A PARTIR DA DATA DE PUBLICACAO NA RPI, DE ACORDO COM O ART. 34 (II) DA LPI PARA QUE SE DE INICIO AO EXAME TECNICO DO PEDIDO.

B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal: dismissal of application maintained