BG98665A - Microcapsulated enterococcus with fatty acid for poultry breeding - Google Patents

Abstract

Fatty acid microspheres of Entertococcus faecium, strains 301 and 202 are dried by revolving disk and used as fodder additive in poultry breeding for growth stimulation and improving the quality of the skeleton.

Description

Growth hormones, in the form of antibiotics, are widely used in poultry farming, namely chickens and turkeys. Growth hormones such as Stafac R and BMD R (bacitracin-methylene-disalicylate) are known antibiotics and are used at sub-therapeutic doses, for example 10 g / ton and 25 g / ton, as feed additives to induce the desired growth of birds. However, the use of antibiotics for these purposes has recently been criticized. One of these criticisms is the possibility that birds may eventually develop an addiction to antibiotics and that the latter may no longer have an effect on stimulating growth. Other health-related objections stem from the unnatural antibiotic ingredients and the aging effects they may have. However, due to the benefits of antibiotics, they are still widely used to improve food absorption, body structure and stimulate growth.

Some bacteria are known to be potentially useful when added to animal feed. These bacteria are useful due to the fact that they create a natural gut microflora. Some companies offer probiotics for sale that contain the desired bacteria. Probiotics, however, * pose some difficulties with regard to product stability.

Typical of the probiotic is that it is used as a feed additive in relatively small doses of about 0.1%. However, unused feed or a feed additive containing a probiotic is often stored by farmers for a long time. This storage is very often under conditions associated with the presence of some moisture and fever. In many cases, the moisture is sufficient to activate the bacteria or start its growth, but it is still insufficient to support it. As a result, they die. This destroys the activity of the probiotic. In other cases, the addition of antibiotics to feed or feed additives containing a probiotic counteracts the bacteria, especially if small amounts of moisture are present and thus the bacteria die again. Therefore, long-term stable storage of feed is a significant problem for probiotics.

In another aspect, when the probiotic is added, for example in chicken feed, the material is usually granulated, the probiotic being added before granulation. The moisture from the steam used during the granulation partially activates the bacteria, but can, as a result of insufficient moisture, destroy them. Also, heat during the granulation process can destroy them. In addition, there is another problem - the acidic environment in the stomach may inactivate the bacteria before they actually reach the intestinal tract. Therefore, there is a continuing need for probiotics, which must release microorganisms only at an appropriate time in the intestine, without first releasing them due to the presence of moisture or an appropriate pH value, which conditions exist in the digestive tract before the small intestine.

BACKGROUND OF THE INVENTION

Some indicators in poultry farming are particularly desirable to achieve, if possible. These include an increased rate of rise in ^w the weight, better feed conversion, carcass composition, and finally uniformity of flock weight. Increased levels of weight gain and feed absorption are, of course, desirable economically. The construction of the carcase is important because the most desirable areas for tissue deposition are the breasts in order to achieve a large amount of selected meat. Thus, weight gain is not only important, but the accumulation of meat on the body is also important. Uniformity in the weight of the flock is important, because the larger the size of the birds, the less manual labor is required and technologists may rely more on machining. On the other hand, if birds vary greatly in size (from very small to very large), even if the total flock weight is the same, smaller and larger birds require much more manual labor because, due to lack of uniformity in size, to apply machining. Therefore, a desirable indicator is uniformity in the weight of the flock with a high percentage distribution over the normal size range so that the chickens can be treated with standard equipment.

A first object of the present invention is to provide a probiotic for poultry farming that does not contain antibiotics but contains only microencapsulated in fatty acid natural microorganisms.

Another object of the invention is to provide a probiotic that contains two microorganisms, namely Enterococcus faecium 301, DSM No. 2. DSM-Nr.4789 and Enterococcus faecium 202, DSM No. 2 DSM-Nr. 4788. DSM is a bacterial culture collection in Germany. DMS stands for Deutsche Sammlung von Mikroorganismen (German Collection of Microorganisms) located in Braunschweig, Germany. These micro-organisms will be deposited with the ATCC once the limitations of the claims have been removed.

Another object of the present invention is to provide a probiotic that gives poultry farming an increased rate of weight gain, better uptake of feed, a greater amount of breast meat and which promotes uniformity in the flock weight over the normal size range.

It is another object of the present invention to provide probiotics suitable as additives in bacterial-containing poultry feed in the form of microspheres by employing a special rotational technique using free fatty acid as a binder.

Another object of the present invention is to provide a probiotic having a persistence for a period of 3 to 6 months without noticeably reducing the number of microorganisms.

Another object of the present invention is to provide a method of rotationally producing spheres of dried bacteria that are uniform in size.

Another object of the present invention is to provide spheres obtained with a rotary disc of dried bacteria that are free-moving and easily processable with feed rations for poultry.

BRIEF DESCRIPTION OF THE DRAWINGS Figures 1, 2 and 3 show graphically the resistance of the strains using stearic acid as a binding agent.

FIG. 4 is a graph showing the distribution of breast meat yield when attempting to eat a probiotic composition of the present invention.

FIG. 5 is a graph showing the distribution of body weight when trying to eat with a probiotic composition of the present invention.

Figures 4 and 5 show control feed tests (i.e., no antibiotic / probiotic), such using an antibiotic and using the probiotic of the present invention.

SUMMARY OF THE INVENTION

The invention provides a method and composition for stimulating poultry growth, comprising adding to the normal fodder rations for poultry a small but growth-stimulating, effective amount of probiotic in the form of dried fatty acid microspheres containing Enterococcus faecium 301, DSM No.DSM-Nr . 4789 and dried fatty acid microspheres containing Enterococcus aecium 202, DSM No.DSM-Nr. 4788, it being preferred that the fatty acid microspheres be obtained by the rotary disc method.

It has been surprisingly found that the stimulation of growth in birds can be achieved by adding to the normal feed rations of a certain amount of fatty acid microspheres with Enterococcus faecium 202, DSM No. DSM-Nr.4788 and a known amount of fatty acid microspheres with Enterococcus faecium 301, DSM No. DSM-Nr.4789. The fatty acid used can be any free acid from C12 to C24> ^{n0 38} preferably stearic acid. Typically, microorganisms are present in equal amounts, but may vary in the range of about 30% to about 70% in favor of one, and the rest is for the other.

It is not sufficiently established why these two microorganisms cause the desired indicators of the present invention, namely increased weight gain, better feed absorption, higher breast meat production and increased homogeneity in flock weight. that they do so when both micro-organisms are used in combination, indicating that they somehow interact with each other, provided that they are applied in the above ratio. It is these combinations of properties that in some way influence and cooperate, create the desired characteristics of the present invention and significantly improve poultry carcass, meat quality and processing.

The amount of probiotic added to feed ration can vary greatly, but is usually in the range of about 227 g to about 908 g per tonne of feed, mainly from about 360 g to 540 g per tonne of feed and typically about 450 g per tonne of feed. The number of microorganisms, i. the number of colony forming units (FEM) per gram in the probiotic may also vary within the range of about 1.10® FEM / g to about 2.10 ⁹ FEM / g, but the preferred amount is about 2.10® FEM / g.

When the probiotic as described above is freely introduced into the feed ration, only then is the combination of the two strains of microorganisms mentioned herein manifested as a growth factor. The growth factors currently used include such antibiotics as Stafac and BMD. The benefits of subtherapeutic levels of antibiotics as growth-stimulant additives can be achieved with naturally occurring microorganisms given in the present invention, provided that the probiotic is obtained according to the present invention and added to the feed according to the method described herein. Indeed, there have been a number of trials suggesting that the combination of probiotic and growth stimulants outweighs their benefits individually, and therefore should be used together if desired. However, in most cases, it is preferable to use the probiotic separately, since one of the purposes of the present invention is to avoid the use of growth stimulants at all.

The method of treatment of micro-organisms is not critical, insofar as they can be kept alive; until they reach the animals and are in such a form that they can be well combined with the feed and of uniform size so that the dosage can be controlled.

A preferred way of meeting these requirements is to include microorganisms in fatty acid microspheres as a binding agent. This process is described in a previous application of the co-inventor Rutherford et al. In this method, the bacteria bind to the heated fatty acid. The temperature of the fatty acid and the time of contact of the fatty acid with the bacteria are controlled to keep the bacteria alive while allowing them to be mixed with the fatty acid. The mixture was placed on a rotating disk, resulting in a microsphere of bacteria and fatty acid acting as a binder. There are several significant advantages to using this method. First, the bacteria remain alive during processing; second, the method combined with the rotary disc technique allows the production of uniformly sized microspheres leading to improved dosing. Third, the nature of the binder, the fatty acid, allows the formation of individual microspheres. This combination of factors contributes to the production of a probiotic with high resistance and maximum efficiency.

In the method of the previous invention, it is important to note that the microspheres are so formed that each of them consists of multiple bacteria in free fatty acid and does not constitute an individual microcapsule for each bacterium coated with a layer or coating of fat acid. This has the advantage of resistance and more effective dosage with bacterial treatment.

The preferred microencapsulating agent is C12 D ° θ24 free fatty acid. Although mixtures of fatty acids are also possible, it is preferable to use a single, pure fatty acid. It is also preferred that this free fatty acid be unsaturated, most preferably stearic acid.

Generally speaking, it is important that the fatty acid has a melting point of less than 75 ° C, preferably in the range of 40 ° C to 75 ° C. At room temperature, it must, of course, be rigid in order to be used as an effective binder. All free fatty acids falling within the foregoing chemical description will satisfy these requirements.

In order to increase the resistance of the product, the bacteria are freeze-dried when incorporated into the product. In this way they can be revitalized by moisturizing.

The microspheres obtained according to the method discussed below typically include from about 50% to over 90% by weight of fatty acid, the remainder being bacterial culture. The preferred range is from about 60% to about 75% fatty acid. If too little fatty acid is used, the binder will be insufficient to provide protection. On the other hand, if too much binder mass is used, it will be too tight and result in insufficient separation of the bacterial culture.

The method as used in the present invention is a method of forming microspheres with a rotating disk. Generally speaking, in this technique, a suspension of the bacteria and fatty acid components, well mixed, is fed at a constant speed in the center of a stainless steel rotating disc. A deflection to the periphery is obtained as a result of the centrifugal force, which forms the microspheres. They are then collected in a refrigerated chamber maintained under ambient or slightly lower temperatures, sorted and prepared for packaging.

While the method of encapsulation with a rotary disc is known, it is not known to make microspheres of a binder without an outer shell, nor is it known to use this method or to encapsulate bacteria free from sublimation. Generally speaking, for a description of the rotary disc encapsulation method, see the publication by Jonson et al. by the Southwestern San Antonio Research Institute, published in Journal of Gas Chromatography, October, 1965, pp. 345-347. In addition, a rotary disc encapsulator suitable for use in the present invention is described in detail in United States Letters Patent, Sparks, 4,675,140, issued June 23, 1987, and entitled Method for Coating Particles For Liquid Droplets. liquid droplets), the reference of which is incorporated herein by reference. However, it is precisely the method described in the previous message that is the most preferred.

It is important to note that the rotational formation of microspheres yields a substantially different product from that of conventional tower spray or microencapsulation. With traditional tower spray, there is a tendency for the particles to adhere, the coating becomes uneven, and thus the product's resistance will be significantly affected, perhaps for days to weeks.

Microencapsulation creates a wrapping around an object, and the bacteria have been shown to be too small, too difficult to keep alive, or to deliver in the same size for practical benefit. In the formation of microspheres, especially with the agents used in the present invention, the resistance of the bacteria, even when subjected to some moisture and antibiotics, will be from three to six months with the viability of the bacteria maintained in evenly distributed particles.

When free fatty acid microspheres are used in the tolerances indicated above, the rotating disc, normally 100 to 150 mm in diameter, rotates at a speed of about 2000 rpm to about 4000 rpm, preferably from about 2500 rpm to about 3200 rpm, a

the feed rate is 50 g to 200 g per minute. Recommended conditions according to the present invention are: use of stearic acid, use of two of the microorganisms described above, 100 mm rotating disc at a rate of 3000 rpm and feed rate of 100 grams per minute of bacterial / stearic acid suspension with composition 35% bacteria, 65% stearic acid. When this is done, a product with a particle size of 75 μm to 300 μm is obtained, preferably a size smaller than 250 μm.

The following examples are given in order to illustrate and not limit the method of the present invention. The examples are described in connection with Figures 1, 2 and 3. Examples 1 to 4 and Figures 1, 2 and 3 refer to the invention of my previous case. Example 5 and Tables 2-10 refer to the method of the present invention for a bird probiotic.

EXAMPLES FOR CARRYING OUT THE INVENTION EXAMPLE 1

Example 1 is related to FIG. 1. It shows the stability of the product for two different strains of Enterococcus faecium at 4 ° C and 27 ° C. As shown in FIG. 1, the resistance of the encapsulated strains of Enterococcus faecium is shown, the encapsulation being carried out with stearic acid by the rotary disc method, with a bacterial culture content of 35% by weight. The formation conditions of the microspheres were as described above, namely, 35/65 bacterial suspension and stearic acid at 65 ° C using a 100 mm disc, a rotating speed of 3000 rpm and at a feed rate of 100 g. a minute. The resulting spheres were placed in heat-sealed envelopes, creating a vapor barrier and weekly destructively tested for colony-forming units (OKEs). It can be seen that the product of the invention maintains excellent colony forming units, counted at storage times up to 70 days.

EXAMPLE 2

Example 2 is to be considered in conjunction with Figure 2. The figure shows the resistance of the individual microencapsulated strains mixed in a typical feed ration in the presence of 3 antibiotics used in poultry farming. The ration consists of the following ingredients:

54% broken into five corn 26% soy flour 2% fish meal 1.5% dicalcium phosphate 1% calcium carbonate 5.5% soybean oil 12% moisture Added are three antibiotics with the following content by weight:

decoquinoate 6% (454 ppm), salinomycin (50 ppm) and monesin sodium (120 ppm).

The bacterial culture is added to the feed mixture in such quantity as to provide about 1.10θ OKE / g of feed. The feed is thermally sealed in bags and incubated at room temperature. Samples are taken weekly to determine the FEC. The graph of FIG. 2 illustrates their excellent durability.

EXAMPLE 3

Example 3 should be considered related to Figure 3. It illustrates the resistance of Enterococcus faecium microspheres in feed in the presence of various antibiotics. The rations consist of 60% finely divided maize, 38% soybean meal and 2% calcium carbonate with a moisture content of about 14%. The bacterial culture was added in an amount providing approximately 10 ⁶ UKE / g of feed and mixed. Ten aliquots of 450 g were sealed in bags at 20 ° C and tested weekly for 16 weeks. Antibiotics are included with the following amounts:

Bacitracin methylene disalicylate 50 g / ton (Bacitracin methylene disalicylate) Carbadox 50 g / ton Chlortetracycline (Chlortetracycline) 200 g / ton Lasalocid 30 g / ton Lincomycin 100 g / ton Neomycin 140 g / ton Oxytetracycline 150 g / ton Sulfamethazine 100 g / ton Tylosin Tylosin 100 g / ton Virginiamycin 20 g / ton ASP 250 100 g / ton Furadox 10 g / ton Table 1 shows given the minimum times to lose logarithmic unit OKE units. TABLE 1 Weekly loss of 1 log unit of OKE counted at 20 ° C 14% wet mixed feed Antibiotic Storage time (days) Control 103 Bacitracin 88 Carbadox 54 Chlortetracycline 60 Lazalocide 57 Lincomycin 75 Neomycin 53 Oxytetracycline 59 Sulfamethazine 62 Tylosin 52 Virginiamycin 112 ASP 250 67 Furadox 53

EXAMPLE 4

Example 4 determined the stability of the product after granulation for use as chicken feed. The conditions for the formation of the microspheres are as described above. The conditions under which the study was submitted were as follows:

Crude protein - not less than ................ 18,0%

Crude fat - not less than ............... 5,0%

Crude fiber - not less than ................... 6,0%

Granules with and without antibiotic (Chlortetracycline 50 g / tonne) are obtained under the following conditions and ingredients:

£ Corn, soy flour, whey, soybean oil, dicalcium phosphate, calcium carbonate, a mixture of minerals (traces), a mixture of vitamins, selenium, copper sulfate. The bacterial culture was added in an amount of approximately 5,105 OKE / g of feed.

The conditioning temperature is 70 ° C and the granules leave the device at 78 ° C. They are stored in unopened bags and tested weekly to determine the number of OKEs.

In each case, the granular product was not adversely affected by the granulation conditions. More specifically, the granular product exhibits a resistance equal to that of the granular product.

^W EXAMPLE 5

560 day-old broiler chickens of the Peterson x Arbor Acres breed were housed in caged poultry farms (Table 2) with straw straw recovered and fed for 45 days. All dead birds in the first 5 days are replaced by birds of the same sex from the same consignment and by the same treatment. The composition of the basic start, growth and end rations is given in Table 3. The start, growth and end rations are formulated to contain 1425,1450 and 1475 kcal / 453 g respectively with 90 g / tonne monezine. Starting rations are given from the first to the 21st day; growth from day 21 to day 42 and end days from day 42 to day 49. The treatments for the different groups are: negative control, ie. compound feed (Control M); selected, encapsulated probiotic bacterial cultures containing Enterococcus faecium 301, DSM No.DSM-Nr.4789 and Enterococcus faecium 202, DSM No.DSM-Nr.4788, each microcapsule with fatty acid by the rotary disc method, as described in Example 1 and each represented by 50% in the probiotic, in an amount of 1.10® OKE / g feed, granulated, designated (Probiotic M); negative control, granular (Control P); 1.10® OKE / g probiotic combined feed, granulated (Probiotic P) and positive control containing 10 g / tonne Virginiamycin granulated (Stafac 10). Starting rations that are granular are crushed. Twelve identical sawmills, each containing 35 male and 35 female chickens, are used for each experimental ration.

Body weight, feed consumption and mortality for each forager are recorded after the first 5 days. Feed utilization, apparent feed utilization and apparent feed utilization based on body weight are calculated for each forklift.

All data are analyzed for variance and differences are determined using the Fisher method of the lowest significant figure.

Prior to the assay, the concentrate containing the probiotic culture was enriched with calcium carbonate. The theoretical numbers for Probiotic M and Probiotic P are 1.10® and 2.10® OKE / g respectively. An 11 g sample of each product is tested twice to determine the actual number of colony forming units. Each sample was coated using the standard Pioneer technique for encapsulated lactic acid bacteria. A mixing test is carried out for each production phase. The test is intended to show that the probiotic is evenly distributed with the appropriate concentration in the feed and has survived granulation. Each batch shall be sampled at the time of packing in bags of 4 equally spaced samples for combined feed and 10 equally spaced samples for processing granulated feed (ie bags 1, 3, 5 ..... 35 , and 39).

Alternative sawmills are sampled during weeks 1 and 4 during processing with non-contaminated feed, and samples are taken at weeks 2 and 6 during the study.

Equal numbers of birds of each sex are killed to determine the individual weight of the breast, body and small intestine, as well as the length of the small intestine. Meat yield ratios and breast weight and gut length are calculated for each bird.

All data are analyzed for variance and the differences are ζ *. determine, using contrasting and estimated postulates for desired effects.

birds from each treatment are transported to the University for an organoleptic evaluation of their taste.

The probiotic, irrespective of its treatment, improves the absorption of feed (P <0.05) compared to Control, and increases (P <0.05) the weight given compared to Control only in concentrated feed (Table 4 ). Probiotic P improves (P> 0.05) the uptake of feed relative to Stafac 10, which is the same (P> 0.05) with Control P.

The product had the desired concentration and composition of the strains (Table 5).

The probiotic is distributed homogeneously in the feed. Probiotic M was at the desired concentration, while Probiotic P was 1 to 1.5 log units higher than the desired amount in the start and growth rations (Table 6). The higher number for Probiotic P was the result of the overprocessing of the product to provide it with sufficient regeneration of the microorganisms in the product after granulation.

Samples of Probiotic P from the sawmills are too close in result to those made immediately upon receipt of the mixtures (Table 7).

However, Probiotic M decreased by two log units in weeks 4 and 6 in growth and final rations.

Probiotic M increased (P <0.05) both breast weight and yield compared to Control M (Table 8), while Probiotic P showed an increase (P> 0.05) compared to Control P. Improvement in compound feed is in agreement with the results obtained from previous experiments. Probiotic P does not show a similar degree of improvement in breast meat production compared to that observed with Probiotic M. This deficiency may be due to improved energy utilization during granulation, resulting in less free space for improvement.

Granulation increases the average weight of the birds by 96 g relative to the compound feed. The probiotic increases the homogeneity in the weight of the birds (Figure 5) and is best in compound feed.

Granulation increases the average weight of the breast by 15 g relative to the compound feed. The probiotic increased mean breast weight and uniformity (Figure 4) compared to Control, with the greatest improvement observed in compound feed. Stafac 10 shows the best distribution of uniformity for pelleted feed.

Granulation increases breast yield by 0.53% compared to compound feed. Probiotic M showed an increase of 0.84% compared to Control M, which is similar to granulation.

Probiotic treatments yielded shorter small intestines (P> 0.05) than controls or Stafac, expressed in true length, in relation to body weight or obesity (Table 9). Probiotic M gives less (P> 0.05) the weight of the small intestine compared to Control M when expressed as actual weight or in relation to the weight of the body or breast. The reduction in weight and length of the small intestine in probiotic treatments implies less energy required to maintain and more energy available for growth, as demonstrated by improved uptake of feed and breast production (Tables 7 and 8).

The birds treated with Probiotic P did not give a residual odor compared to those treated with Stafac 10 (Table 10). On the second attempt with Probiotic P, an increase in the odor of the thigh / leg was observed compared to Control R. However, this increase in odor was not observed in the first attempt.

TABLE 2

ASSESSMENT OF THE PILCHRIDGERS

Processing Number of sawmills Control P Probiotic P Stafac 10 Control M Probiotic M 2.6.15.17.22.26.104.109.113.117.122.126 4,8,12,16,21,28,105,106,112,118,125,130 5,7,11,18,23,27,101,107,111,116,123,129 3.9.13.20.24.30.102.108.114.119.121.127 1,10,14,19,25,29,103,110,115,120,124,128

The size of the sawmills is 10.5 x 40 cm with one feed pipe, hanging drinker, pits for fertilizer, cooling system, well insulated, air heater, side curtains of the building.

TABLE 3

BASIC RATES COMPOSITIONS (in%)

Ingredients Production phases

startup growth extreme Ground corn 65.37 67.89 74.29 Soy flour 25.58 25.53 17,83 Meat and bone meal 3.00 3.00 3.00 Fat 3,36 3,32 2.59 Phosphate is decontaminated 0.95 0.79 0.73 Calcium carbonate 0.61 0.62 0.63 The sol 0.35 0.31 0.32 Traces of minerals 0.05 0.05 0.05 Methionine 0.39 0.28 0.33 Lysine 0.19 0.06 0.18 A mixture of vitamins 0.05 0.05 0.05

TABLE 4 DATA ON THE PRODUCTION OF THE BAKERS

Contr. D wounded Comb. feed Fig. R. The staff Control The image of M. Weight, Mr 2170,8® 2178,9® 2170,8® 2056,6b 21208 Awareness for feed 847.6 ^b 827,6a 840,3ab 868,4c 840,78b Private feed ¹ 829.9 ^b 810,48 818,6ab 854.8 ° 820,88b Private Led Feed based on weight ² 815,8b 7958 804,18b 859.3C 812,2b Mortality,% 4.40 4,64 5.95 3,33 5.61

¹ Apparent feed absorption = Total feed / (live + dead weight).

² Apparent absorption of feed based on weight = Pvc. - (weight 4.60) / 61 abc p <0.05

TABLE 5 PRODUCT CHARACTERISTICS Processing No. No. Rel. of the strains * OKE / g product * SF 202: SF 301 Probiotic P Probiotic Μ 5,75,105 1,01,105 50:50 9,54,107 9,60,107 57:43

TABLE 6 FEED MIXER TEST AND REGENERATION TEST

Production phases Comb. feed Granules Recovery ¹ and processing OKE / g feed % of total feed Starting Control P - 1.06.10 ³ - Probiotic R. 2,02.10® 1,67.10® 98.69 Column 10 - 6,46.10 ³ - Control M 2,51.10 ³ Probiotic M 1,34,105 Growing Control P - 4,86.10 ² - Probiotic R. 3,89.10® 1,09.10® 91.62 Column 10 5,25.10 ⁴ 6,42.10® Control M 1,00.102 Probiotic M 1,48.10 ⁵ Extreme Control P 8,50.10 ² 1,11.10 ³ - Probiotic 7,04.10® 4,91.105 117.43 Column 10 8,80.10 ³ 1,79.10® - Control M 8,92.10 ² Probiotic M 1,33.10 ⁵ Medium Control P 8,50,102 8,28,102 - Probiotic R. 8,21,105 9,64.10® 118.09 Column 10 2,15.10® 9,05.10 ³ Control M 8,72.102 Probiotic M 1,38.10 ⁵

¹ Regeneration is calculated on data converted to log

TABLE 7

EVALUATION OF THE QUALITY OF THE SAWER

Weeks Processing 1 2 4 6 OKE / g feed Probiotic control P Stafac 10 Control M Probiotic M 3 .78.102 9.23.10 ⁵ 8.73.102 3.46.102 1.43.10 ⁵ 3.87.102 9.37.10 ⁵ 1.29.102 1.26.10 ² 1.25.10 ⁵ 8.60.102 8.77.10 ⁵ 6.46.102 2.79.103 1.75.103 2.21.102 4.08.102 8.48.10 ⁵ 8.96.10 ⁵ 8.63.102 8.89.102 2.00.102 5.08.102 1.00.103 2.32.10 *

TABLE 8

EVALUATION OF BREAST COOKING

Control D wounded Comb. feed FIG * Staff Control Imag Body weight, 2240,7 2230,1 2195,9 2143,8 2149,9 Breast weight, g 234,4® 239,6® 232,0® 213,3 ⁵ 229,6® (% by weight) 10.51® 10.68® 10.58® 9,93b 10,67®

^a b P <0.05

Tea *

TABLE 9

WEIGHT AND LENGTH OF THE THIN GUT

D wounded

Comb. feed

Contr. Fig. R. The staff Control Figure 1 Body weight, 2240,7 2230,1 2195,9 2143,8 2149,9 Breast weight, 234.4 ^a 239.6 ^a 232,0® 213.3 ^b 229,6a Small intestine weight, g 92.6 93,3 93,4 91,4 87,4 Length of soot, cm 193,8 191,3 194,6 193,3 191,3 Small intestine, g / cm 0,479 0,488 th most common 0.481 0,474 th most common 0,457 th most common Small intestine, weight g / 100 g body weight 4.13 4,18 4.25 4.39 4.09 Small intestine, length cm / 100g body weight 8.64 8.66 8.86 9.01 8.89 Small intestine, weight g / 100 g breast meat 39,50 38.93 40,25 42.63 38.06 Small intestine, length cm / 100 g breast meat 82.67 79.84 83,87 90.62 83,31

^ab P <0.05

TABLE 10

EVALUATION OF TASTE QUALITIES

Yes D hole for comparison Number of correct determinations ¹ Experience 1 Experience 2 Combined Hip / leg Stafac - Control P 6 3 9 Stafac - XINOC R 3 4 7 Probiotic P - Control P 2 8 * 10 Chest Stafac - Control P 2 6 8 Stafac - XINOC R 1 3 4 Probiotic P - Control P 5 4 9

* The evaluators were able to find a statistically significant number of times for the non-standard sample ¹ The number of correct non-standard sample determinations required to accept the test at 5% is 7 for η = 10 and 11 for η = 20.

Claims (15)

1. A method of stimulating growth in birds, characterized in that it includes: adding to the normal poultry feed rations a small but growth-stimulating effective amount of a probiotic consisting mainly of viable, persistent, dried fatty acid microspheres with Enterococcus faecium 301, ATCC No.________ and viable, stable, dried microspheres Enterococcus faecium 202, ATCC No. 2 Chg. · 2. The method of claim 1, wherein the fatty acid spheres are obtained using a rotary disc. The method of claim 2, wherein the probiotic is from about 30% to about 70% of one of said fatty acid microspheres, the remaining amount being the other. The method of claim 3, wherein the fatty acid is C12 to C24 free fatty acid. 5. The method of claim 4, wherein the fatty acid is stearic acid. geWr, I * The method of claim 1, wherein each of said streptococci is present in approximately equal amounts. The method of claim 1, wherein the amount of probiotic added to feed ration is from about 227g to about 907g per tonne of feed. The method of claim 7, wherein the amount of probiotic is from about 363 g to about 545 g per tonne of feed. The method according to claim 7, characterized in that the number of microorganisms in the probiotic is from about 1.10 ⁵ FU / g to about 2.10θ FU / g A method according to claim 9, characterized in that the number of microorganisms in the probiotic is about 1.10 ⁵ FU / g. 11. Probiotic composition for stimulating growth in poultry, consisting mainly of viable, resistant, dried fatty acid microspheres with Enterococcus faecium 301 and viable, resistant, dried fatty acid microspheres with Enterococcus faecium 202. The probiotic of claim 11, which has from about 30% to about 20% of one of said streptococci, the other being the other. The probiotic of claim 12, wherein the free fatty acid is a C 2 to C 24 free fatty acid. The probiotic of claim 13, wherein the fatty acid is esteric acid. The probiotic of claim 14, wherein the streptococcal microorganisms are present in approximately equal amounts.