BG2004U1 - Remedy for immunoprophylaxis of colibacillosis in livestock - Google Patents

Abstract

The utility model relates to a remedy for immunoprophylaxis of colibacillosis in livestock, in particular a remedy for oral administration, having an effect simultaneously for strengthening and stabilizing the immune system, and for neutralizing the inducer of the disease. It includes a lyophilized serum preparation against the antigens Ent + LТ, ST, F4 аb, ас, ad, F5, ST, F6, F41 with a titer of 1:800 and lyophilized probiotic preparation in a ratio of 1:2. The lyophilized probiotic preparation, in turn, includes the strains Lactobacillus acidophilus NBIMCC 1379, Lactobacillus delbrueckii ssp. bulgaricus NBIMCC 287, and Bifidobacterium bifidum NBIMCC 1370, each in a quantity of 2 х 1010 live cells/ml. 1 claim

Description

(54) AGENT FOR IMMUNOPROPHYLAXIS OF COLIBACTERIOSIS IN AGRICULTURAL ANIMALS

Field of technology

The utility model relates to an agent for the immunoprophylaxis of colibacillosis in farm animals, in particular to an agent for oral administration.

BACKGROUND OF THE INVENTION

The causative agent of the acute infectious disease of young animals - colibacillosis are the bacteria Escherichia coli. These bacteria, including their enteropathogenic serotypes, are normal inhabitants of the intestinal tract of healthy adult animals and are excreted in large quantities in the faeces. But in cases where the body's defense system is disrupted, the bacteria begin to multiply rapidly and enter the intestinal mucosa, releasing endo and exotoxins. Toxins affect the intestinal mucosa, causing a state of enterosorption - abundant secretion of water and electrolytes and increase intestinal peristalsis. In case of insufficient activity of the protective mechanisms of the intestinal wall and, above all, phagocytosis, Escherichia coli penetrates the lymphatic and blood system and causes a septic process. Diarrhea causes dehydration of the tissues; the multiplication of bacteria and the flooding of the body with toxic products of their vital activity and tissue breakdown suppress the activity of the nervous system, which towards the end of the disease leads to a severe comatose state.

Susceptible to colibacillosis are mainly young animals of all agricultural species. At first, weak animals get sick. After several passages through their body, the virulence of the bacteria increases; they begin to cause disease in completely normal newborn animals, and in turn the sick pollute the environment abundantly with rare feces and become the main source of the pathogen.

The possibility of the transition of the saprophytic bacterium Escherichia coli in a pathogenic state calls for the need to pay serious attention to the resilience of the body of newborns and, accordingly, to the stability of their immune system. Therefore, it is necessary to pay attention to the conditions of feeding and rearing of pregnant mothers and newborns. It is recommended (Epizootology, ed. Sosov, RF, M, Kolos, 1974) to strengthen the resistance of newborns to give milk fermented with acidophilic yeast, as well as a broth culture of lactic acid bacteria.

On the other hand, as a main tool for breaking the infectious chain, it is recommended to introduce into the body of animals biological preparations against the pathogen such as vaccines, toxoids, serum preparations and others. One such preparation is the inactivated polyvalent vaccine for the control of necrotic enteritis and cholienterotoxicosis, according to AC BG 50963, which is a mixture of inactivated cultures of specific enterotoxigenic strains of E. coli and strains of Clostridium perfringens. This vaccine works only against the pathogen, does not help strengthen the body's immune system so as to prevent the possibility of disease. From patent RU 2291709 is also known a preparation for immunoprophylaxis of young animals, which is a combination of serum and probiotic administered to animals sequentially by injection and orally for about 10 days. However, this preparation was prepared for the purpose of immunoprophylaxis of respiratory diseases in calves.

The purpose of the utility model is to propose a means of immunoprophylaxis of colibacillosis in farm animals, in particular a means of oral administration, acting both to strengthen and stabilize the immune system and to neutralize the pathogen.

Technical essence of the utility model

In essence, the immunoprophylaxis agent for colibacillosis, according to the utility model, is a combination of lyophilized serum preparation and lyophilized probiotic preparation in a ratio of 1: 2, especially serum preparation against antigens Ent + LT, ST, F4 ab, ac, ad, F5, ST, F6, F41 with tiger 1: 800 and probiotic preparation of special

2004 UI selected strains of Lactobacillus acidophilus NBPMCC 1379, deposited on 03.01.2010, Lactobacillus delbrueckii ssp. bulgaricus NBMPMK 287, deposited on 14.01.2008 and Bifidobacterium bifidum NBPMKK 1370, deposited on 03.01.2010, each in an amount of 2 x 1010 live cells / ml.

The serum preparation and probiotic preparation included in the means for immunoprophylaxis of colibacillosis in farm animals, according to the utility model, act unidirectionally to neutralize the enteropathogenic microorganisms, and at the same time the immune system of the organism is strengthened and stabilized; the serum preparation on the one hand provides an immediate effect, and on the other - through the probiotic this effect is maintained for a long period of time.

The immunoprophylaxis agent for colibacillosis in farm animals, according to the utility model, is available in lyophilized form in vials and / or sachets, which facilitates its administration. Prior to use, the contents of the vial are dissolved in milk or water and can be administered individually to each animal orally or by drinking water or food to many animals at the same time. This allows its use at an early age, to avoid antibiotic treatment and to achieve a long-lasting effect in order to eliminate the risk moments in suckling pigs, during the weaning period or during the transition from one age group to another. The tool, according to the utility model, provides an increase in the growth of animals at a young age and stimulates the stabilization of their immune system in order to prevent other infectious diseases.

The utility model is illustrated by the following exemplary embodiments without limiting its scope.

Example 1.

According to one embodiment of the utility model, one vial, representing one therapeutic dose of the agent for immunoprophylaxis of colibacillosis in farm animals, contains 1.0 g of lyophilized serum preparation against E. coli antigens Ent + LT, ST, F4 ab, ac, ad, F5, ST, F6, F41 with tiger 1: 800 and 2.0 g of lyophilized probiotic preparation.

The serum, in turn, was prepared according to the technology described in AC BG 50963, involving selected and typed enterotoxigenic strains of E. coli, followed by lyophilization. It is specially designed against the antigens Ent + LT, ST, F4 ab, ac, ad, F5, ST, F6, F41, where:

Ent + means enterotoxigenic

LT means thermolabile

ST means thermostable

F means fimbrial

The numbers 4, 5, 6 and 41 denote the different fimbrial antigens, and the lowercase letters ab, ac, ad denote the different epitopes of the antigens.

Serum was obtained after hyperimmunization of animals with these antigens. After extraction, it is subjected to titration to determine activity of not less than 1: 800 against each antigen, titration standardization and lyophilization.

The probiotic preparation in turn contains specially selected probiotic strains of Lactobacillus acidophilus NBPMCC 1379, Lactobacillus delbrueckii ssp. bulgaricus NBPMKK 287 and Bifidobacterium bifidum NBPMKK 1370, each in an amount of 2 x 1010 live kpetki / ml. The culture of probiotic microorganisms, according to the utility model, is lyophilized under mild conditions so that it can be mixed with the lyophilized serum preparation, the mixture can be placed in a vial or sachet and given orally to the animals.

Before use, the contents of one vial (one treatment dose) are dissolved directly in 10 ml of milk or water and given individually to each animal by mouth. The same can be given to animals through drinking water or food.

Table 1 illustrates the effect of the administration of the colibacillosis agent according to the present utility model on pigs compared to pigs treated with serum only and probiotic only. The results show that the treatment (experiment № 3) of 12 animals with the product, according to the utility model, leads to complete recovery of 11 pcs. and only one animal remains in a chronic form of the disease.

2004 UI

Table 1

№ Number of treated Serum only (ml) The result Only with probiotic The result 1 18 - - - - 5 ml --- recovered -14, chronic form - 3 died -1 - - 2 10 - 3x1 vial every two days healed - 4; chronic form- 4 died-2 3 12 5 ml + _ 3x1 vial every two days healed -11; chronic form-1

Claims

Claims (1)

Claims 1. An agent for immunoprophylaxis and colibacillosis in farm animals, in particular a means for oral administration based on 20 serum and probiotic preparations, characterized in that it comprises a lyophilized serum preparation against the antigens Ent + LT, ST, F4 ab, ac, ad, F5, ST, F6, F41 with a titer of 1: 800 and a lyophilized probiotic preparation containing strains of Lactobacillus acidophilus NBPMCC 1379, Lactobacillus delbrueckii ssp. bulgaricus NBPMKK 287 and Bifidobacterium bifidum NBPMKK 1370, each in an amount of 2 x 1010 live cells / ml, in a ratio of 1: 2.