BG100068A - Method of detecting the presence of and converting of a polypeptide - Google Patents

Method of detecting the presence of and converting of a polypeptide

Info

Publication number
BG100068A
BG100068A BG100068A BG10006895A BG100068A BG 100068 A BG100068 A BG 100068A BG 100068 A BG100068 A BG 100068A BG 10006895 A BG10006895 A BG 10006895A BG 100068 A BG100068 A BG 100068A
Authority
BG
Bulgaria
Prior art keywords
growth hormone
polypeptide
converting
detecting
mercaptan
Prior art date
Application number
BG100068A
Other languages
Bulgarian (bg)
Inventor
Hans Sorensen
Thorkild Christensen
Original Assignee
Novo Nordisk A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk A/S filed Critical Novo Nordisk A/S
Publication of BG100068A publication Critical patent/BG100068A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormones [GH] (Somatotropin)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/575—Hormones
    • G01N2333/61—Growth hormones [GH] (Somatotropin)

Abstract

По метода хидрофобно производно на растежния хормон може да бъде открито чрез хидрофобна хроматография и елуиране с амониев сулфат в качеството на градиент, последвано от пептидно картиране. Производното може да бъде третирано с меркаптаново съединение за превръщането му в нативна форма на растежния хормон. За предпочитане е растежният хормон да бъде човешки, а меркаптановото съединение цистеин вконцентрация 1-2 мм.By the hydrophobic growth hormone derivative method, it can be detected by hydrophobicity chromatography and eluting with ammonium sulfate as a gradient followed by peptide mapping. The derivative can be treated with a mercaptan compound to convert it into a native form of growth hormone. Preferably, the growth hormone is human, and the mercaptan cysteine compound at a concentration of 1-2 mm.

BG100068A 1993-04-20 1995-10-17 Method of detecting the presence of and converting of a polypeptide BG100068A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK93445A DK44593D0 (en) 1993-04-20 1993-04-20 PROCEDURE FOR PREPARING A POLYPEPTIDE
PCT/DK1994/000157 WO1994024157A1 (en) 1993-04-20 1994-04-19 A method of detecting the presence of and converting of a polypeptide

Publications (1)

Publication Number Publication Date
BG100068A true BG100068A (en) 1996-12-31

Family

ID=8093625

Family Applications (1)

Application Number Title Priority Date Filing Date
BG100068A BG100068A (en) 1993-04-20 1995-10-17 Method of detecting the presence of and converting of a polypeptide

Country Status (14)

Country Link
EP (1) EP0695310A1 (en)
JP (1) JPH08508735A (en)
KR (1) KR960701891A (en)
AU (1) AU6535594A (en)
BG (1) BG100068A (en)
CA (1) CA2160663A1 (en)
CZ (1) CZ272895A3 (en)
DK (1) DK44593D0 (en)
FI (1) FI955000A0 (en)
HU (1) HUT73320A (en)
IL (1) IL109347A0 (en)
NO (1) NO954181D0 (en)
PL (1) PL311198A1 (en)
WO (1) WO1994024157A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9802454D0 (en) 1998-07-08 1998-07-08 Pharmacia & Upjohn Ab Production of peptides
US20040048315A1 (en) * 2002-08-28 2004-03-11 Pharmacia Corporation Method for the preparation of growth hormone and antagonist thereof having lower levels of isoform impurities thereof
CN1697839B (en) * 2002-08-28 2010-10-27 法玛西亚公司 Method for the preparation of growth hormone and antagonist thereof having lower levels of isoform impurities thereof
ZA200502320B (en) * 2002-09-20 2006-10-25 Pharmacia Corp Process for decreasing aggregate levels of pegylated protein
SI1545428T1 (en) * 2002-09-20 2010-03-31 Pharmacia Corp Process for decreasing aggregate levels of pegylated protein
EP1833841B1 (en) 2004-12-29 2010-04-07 Novo Nordisk Health Care AG Method for preventing formation of trisulfide derivatives of polypeptides
US9005926B2 (en) 2009-10-02 2015-04-14 Biogen Idec Ma Inc. Methods of preventing and removing trisulfide bonds
US9562252B2 (en) 2011-05-13 2017-02-07 Biogen Ma Inc. Methods of preventing and removing trisulfide bonds

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4985544A (en) * 1987-08-04 1991-01-15 Kyowa Hakko Kogyo Co., Ltd. Process for renaturing fish growth hormone
US5079230A (en) * 1988-09-12 1992-01-07 Pitman-Moore, Inc. Stable bioactive somatotropins
WO1992004382A1 (en) * 1990-09-05 1992-03-19 Bunge (Australia) Pty. Ltd. Solubilization of proteins in active forms
US5151501A (en) * 1991-12-20 1992-09-29 American Cyanamid Company Method for solubilization and naturation of somatotropins utilizing sulfolane

Also Published As

Publication number Publication date
CZ272895A3 (en) 1996-03-13
IL109347A0 (en) 1994-07-31
NO954181L (en) 1995-10-19
DK44593D0 (en) 1993-04-20
CA2160663A1 (en) 1994-10-27
FI955000A (en) 1995-10-19
HUT73320A (en) 1996-07-29
NO954181D0 (en) 1995-10-19
EP0695310A1 (en) 1996-02-07
WO1994024157A1 (en) 1994-10-27
AU6535594A (en) 1994-11-08
FI955000A0 (en) 1995-10-19
JPH08508735A (en) 1996-09-17
PL311198A1 (en) 1996-02-05
KR960701891A (en) 1996-03-28
HU9503020D0 (en) 1995-12-28

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