BE902745A - Methode de dosage par bioluminescence a l'aide d'enzymes immobilisees. - Google Patents

Methode de dosage par bioluminescence a l'aide d'enzymes immobilisees.

Info

Publication number
BE902745A
BE902745A BE85215257A BE215257A BE902745A BE 902745 A BE902745 A BE 902745A BE 85215257 A BE85215257 A BE 85215257A BE 215257 A BE215257 A BE 215257A BE 902745 A BE902745 A BE 902745A
Authority
BE
Belgium
Prior art keywords
assay
assay method
tube
bioluminescence
immobilized enzymes
Prior art date
Application number
BE85215257A
Other languages
English (en)
Original Assignee
Remacle Jose
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Remacle Jose filed Critical Remacle Jose
Priority to BE85215257A priority Critical patent/BE902745A/fr
Publication of BE902745A publication Critical patent/BE902745A/fr
Priority to AU59674/86A priority patent/AU592096B2/en
Priority to JP50336186A priority patent/JPS63500631A/ja
Priority to DE8686903170T priority patent/DE3671646D1/de
Priority to PCT/BE1986/000020 priority patent/WO1987000198A1/fr
Priority to EP86903170A priority patent/EP0229085B1/fr
Priority to US07/031,470 priority patent/US4927752A/en
Priority to AT86903170T priority patent/ATE53234T1/de
Priority to ES556943A priority patent/ES8708162A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/805—Test papers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Utilisation de la luciférase adsorbée sur la paroi d'un tube de dosage afin de doser certaines enzymes ou leurs substrats par bioluminescence, L'invention consiste à immobiliser sur un tube qui servira au dosage par bioluminescence la luciférase et une des NADH ou NADPH:FMN oxydoréductase. Cette adsorption permettra de doser le NADH ou le NADPH en solution et de ce fait une autre déshydrogénase ou son substrat. Cette déshydrogénase pourra aussi etre co-immobilisée sur le tube avec les 2 autres enzymes, ce qui permet un dosage de son substrat dans la solution de réaction. Une quatrième enzyme, pourrait également etre ajoutée au système.
BE85215257A 1985-06-26 1985-06-26 Methode de dosage par bioluminescence a l'aide d'enzymes immobilisees. BE902745A (fr)

Priority Applications (9)

Application Number Priority Date Filing Date Title
BE85215257A BE902745A (fr) 1985-06-26 1985-06-26 Methode de dosage par bioluminescence a l'aide d'enzymes immobilisees.
AU59674/86A AU592096B2 (en) 1985-06-26 1986-06-19 Support used in bioluminescent dosing of enzymes, substrates or enzymatic inhibitors
JP50336186A JPS63500631A (ja) 1985-06-26 1986-06-19 酵素、基質または酵素阻害剤の生物発光投与方法、その投与に使用する支持体、その投与の利用およびその支持体の利用
DE8686903170T DE3671646D1 (de) 1985-06-26 1986-06-19 Traeger zur verwendung in biolumineszenz-bestimmungsverfahren von enzymen, substraten oder enzym-hemmungskoerpern.
PCT/BE1986/000020 WO1987000198A1 (fr) 1985-06-26 1986-06-19 Support pour utilisation dans le dosage par bioluminescence d'enzymes, de substrats ou d'inhibiteurs enzymatiques
EP86903170A EP0229085B1 (fr) 1985-06-26 1986-06-19 Support pour utilisation dans le dosage par bioluminescence d'enzymes, de substrats ou d'inhibiteurs enzymatiques
US07/031,470 US4927752A (en) 1985-06-26 1986-06-19 Support used in bioluminescent dosing of enzymes, substrates or enzymatic inhibitors
AT86903170T ATE53234T1 (de) 1985-06-26 1986-06-19 Traeger zur verwendung in biolumineszenzbestimmungsverfahrenvon enzymen, substraten oder enzym-hemmungskoerpern.
ES556943A ES8708162A1 (es) 1985-06-26 1986-06-25 Procedimiento de dosificar enzimas por bioluminiscencia y perfeccionamientos en los soportes para su realizacion

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BE902745 1985-06-26
BE85215257A BE902745A (fr) 1985-06-26 1985-06-26 Methode de dosage par bioluminescence a l'aide d'enzymes immobilisees.

Publications (1)

Publication Number Publication Date
BE902745A true BE902745A (fr) 1985-10-16

Family

ID=25654640

Family Applications (1)

Application Number Title Priority Date Filing Date
BE85215257A BE902745A (fr) 1985-06-26 1985-06-26 Methode de dosage par bioluminescence a l'aide d'enzymes immobilisees.

Country Status (2)

Country Link
US (1) US4927752A (fr)
BE (1) BE902745A (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996032499A1 (fr) * 1995-04-13 1996-10-17 Mischke, Hans-Rudolf Procede de determination quantitative d'inhibiteurs d'oxydoreductases a effet reversible

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3928209A1 (de) * 1989-08-25 1991-02-28 Boehringer Mannheim Gmbh D-malat-bestimmung
FI913443A0 (fi) * 1991-07-17 1991-07-17 Rolf Kroneld Metod, apparat och indikator foer att indikera flyktiga kolvaeten eller miljoegifter i vatten och vaetskor.
US5145956A (en) * 1991-12-11 1992-09-08 Glycomed Incorporated Method for separation of anionic oligosaccharides
US5268288A (en) * 1992-03-25 1993-12-07 North Carolina State University Mannitol oxidoreductase isolated from vascular plants
DE69331597T2 (de) * 1992-11-25 2002-09-12 Chisso Corp Methoden und Verwendung von poly-L-lysine als Enzymschutzmittel
US5624813A (en) * 1994-04-21 1997-04-29 Mahant; Vijay K. NAD(P)+ /NAD(P)H based chemiluminescent diagnostics
US20080050762A1 (en) * 2001-02-13 2008-02-28 Corey Michael J Methods and compositions for coupled luminescent assays
US20070264667A1 (en) * 2003-09-03 2007-11-15 Robert Gellibolian Method and system for assaying transferase activity
KR100571822B1 (ko) * 2003-10-30 2006-04-17 삼성전자주식회사 마이크로 ｐｃｒ 장치, 그를 이용한 핵산의 증폭방법 및ｐｃｒ 증폭 산물의 농도를 측정하는 방법

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4234681A (en) * 1978-07-21 1980-11-18 The Regents Of The University Of California Immobolized light emitting systems
EP0134307B1 (fr) * 1983-07-29 1989-04-19 Henning Berlin GmbH Chemie und Pharmawerk Surfaces de matières synthétiques préactivées pour l'immobilisation de matériaux organo-chimiques et biologiques, leur procédé de préparation et leur application

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996032499A1 (fr) * 1995-04-13 1996-10-17 Mischke, Hans-Rudolf Procede de determination quantitative d'inhibiteurs d'oxydoreductases a effet reversible

Also Published As

Publication number Publication date
US4927752A (en) 1990-05-22

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Legal Events

Date Code Title Description
CH Change of patent owner

Owner name: *BIOTEC S.A.

Effective date: 19850626

RE Patent lapsed

Owner name: BIOTEC S.A.

Effective date: 19900630