AU770542B2 - Topical organic ectoparasiticidal formulations - Google Patents

Description

WO 01/12156 PCT/USOO/19549 -1- TOPICAL ORGANIC ECTOPARASITICIDAL FORMULATIONS This invention relates to topical organic ectoparasiticidal formulations.

Ectoparasites such as fleas, blowflies, lice, ticks and mites can seriously affect productivity in the domesticated animal industries. Further, such parasites cause disease and discomfort for pets and other companion animals. Ectoparasites are often controlled by topically applying an insecticide or mixture of insecticides onto the animal. Topical ectoparasite control agents are usually applied in liquid formulations.

The formulations can be applied by spot-on application, plunge or spray dipping, jetting with a hand held spray or in a race, or as a back-line spray or pour-on.

A particular problem with topical formulations is poor migration from the site of application. In the sheep industry, for example, treatment for ectoparasites is commonly carried out in the early season within 24 hours after shearing, or, less frequently, later in the season when the wool is longer. Especially with early season treatments, when the topical formulation is applied along the dorsal midline or backline of the animal, the insecticide component of currently available commercial formulations migrates very poorly from the application site. Typically less than of the applied insecticide diffuses away from the application site within the first days. Thus, extensive areas of the animal's skin and/or hair may receive sublethal concentrations of the insecticide. These areas remain susceptible to damaging invasion by ectoparasites.

To overcome the inadequate control caused by poor migration of the insecticide, it has become common in the industry to apply relatively large amounts of insecticide. This practice introduces unwelcome costs, results in the presence of insecticide residues in certain animal products (such as wool and wool byproducts) and increases the potential of environmental pollution. It also increases the risk of unwanted and unnecessary exposure to pesticides to animal handlers and farmers treating the animals.

Solvent-based formulations have received attention in recent times in the search for greater insecticide mobility that would allow the same insecticidal effect to be achieved with less insecticide in the formulations. Up to the present, there 2 has been little success in identifying a solvent that materially enhances the spread of insecticides that are applied using a spot-on or pour-on method.

According to a first embodiment of the present invention there is provided a topical ectoparasiticidal formulation comprising from 0.1 to 25 weight percent of a spinosyn, or a physiologically acceptable derivative or salt thereof; from 25 to 99 weight percent of isopropyl myristate and from 0.6 to 40 weight percent of a miscibilizing agent compatible with organic solvent systems, selected from acetic, lauric, octanoic, oleic and benzoic acids and N-methyl-2-pyrrolidinone.

According to a second embodiment of the present invention there is provided an article of manufacture, comprising packaging material and contained within said packaging material a topical formulation for controlling an ectoparasite infestation on a small ruminant or companion animal, wherein said formulation comprises a topical unit dose of a formulation of the first embodiment; and, wherein said packaging material comprises a label or package insert with instructions for topically administering the dose to the animal.

According to a third embodiment of the present invention there is provided a topical ectoparasiticidal formulation comprising from 0.1 to 25 weight percent of a spinosyn, or a physiologically acceptable derivative or salt thereof; from 25 to 99 weight percent of isopropyl myristate and from 0.6 to 40 weight percent of a miscibilizing agent compatible with organic solvent systems, selected from acetic, lauric, octanoic, oleic and benzoic acids and N-methyl-2-pyrrolidinone when used to control an ectoparasite infestation on a small ruminant or companion animal.

According to a fourth embodiment of the present invention there is provided a topical ectoparasiticidal formulation comprising from 0.1 to 25 weight percent of a spinosyn, or a physiologically acceptable derivative or salt thereof; from 25 to 99 weight percent of isopropyl myristate and from 0.6 to 40 weight precent of a miscibilizing agent compatible with organic solvent systems, selected from acetic, lauric, octanoic, oleic and benzoic acids and N-methyl-2-pyrrolidinone for use in controlling an ectoparasite infestation on a small ruminant or companion animal.

According to a fifth embodiment of the present invention there is provided a method of controlling an ectoparasite infestation on a small ruminant or companion animal, comprising topically applying to the animal a formulation of the first embodiment third or fourth embodiment.

According to a sixth embodiment of the present invention there is provided use of 35 from 0.1 to 25 weight percent of a spinosyn, or a physiologically acceptable derivative or [R:\LIBVV]03350speci.doc:njc salt thereof in combination with from 25 to 99 weight percent of isopropyl myristate in combination with from 0.6 to 40 weight percent of a miscibilizing agent compatible with organic solvent systems, selected from acetic, lauric, octanoic, oleic and benzoic acids and N-methyl-2-pyrrolidinone for the manufacture of a medicament to control an ectoparasite infestation on a small ruminant or companion animal.

This invention describes insecticidal formulations that can be applied topically to animals and that have the advantage of permitting the active ingredient to spread over the surface of the skin and/or hair of the treated animal, thereby providing more extensive coverage of the insecticide. These formulations, therefore, provide greater inhibition or to eradication of ectoparasites with smaller amounts of insecticide.

The topical ectoparasiticidal formulations of this invention comprise an ectoparasiticide, a spreading agent and optionally a miscibilizing agent. More specifically, the invention relates to a topical ectoparasiticidal formulation comprising from about 0.1 to about 25 weight percent of an ectoparasiticide, from about 25 to about 99.9 weight percent of a (C 3

-C

6 branched alkyl (C 10

-C

20 alkanoate spreading agent, and optionally up to about 70 weight percent of a miscibilizing agent compatible with organic solvent systems.

An exemplary topical ectoparasiticidal formulation of this invention is one wherein the ectoparasiticide is a spinosyn, or a physiologically acceptable derivative or salt thereof.

This invention also describes a method of controlling an ectoparasite infestation on a small ruminant or companion animal, comprising topically applying to the hair and/or skin of the animal a formulation comprising from about 0.1 to about 25 weight percent of a spinosyn, or a physiologically acceptable derivative or salt thereof, from about 25 to S 25 99.9 weight percent isopropyl myristate, and from 0 to about 70 weight percent of a miscibilizing agent compatible with organic solvent systems.

The invention also describes an article of manufacture, comprising packaging material and a topical formulation for controlling an ectoparasite infestation on a small ruminant or companion animal contained within said packaging material, wherein said formulation comprises q* a topical unit dose of a formulation comprising 0.1 to about 25 weight percent of an ectoparasiticide, from about 25 to about 99.9 weight percent of a (C 3

-C

6 branched alkyl (C 1 0

-C

2 0 alkanoate spreading agent, and optionally up to about 70 weight percent of a miscibilizing agent compatible with organic solvent systems; and, [R:\LIBVV]03350speci.doc:njc wherein said packaging material comprises a label or package insert with instructions for topically administering the dose to the animal.

This article of manufacture or kit is particularly appropriate when the companion animal is a dog or a cat. The timing of administering the doses will generally be every days. Each kit typically contains a sufficient number of doses to control the ectoparasite infestation for a period of several months.

This invention further describes a topical formulation for controlling an ectoparasite infestation on a small ruminant or companion animal comprising a spinosyn, or a derivative or salt thereof, and a spreading agent substantially as hereinbefore described to with references to any one of the Examples.

Examples of small ruminant animals are a sheep, a goat or a camellid.

The term "companion animal" includes dogs, cats, horses and other pets owned and maintained in close association with humans as part of the human-animal bond.

The term "controlling" as used herein refers to either ameliorating or eliminating a current infestation or preventing an infestation in a susceptible host.

Many insecticidal agents are useful in the formulations of this invention. Indeed, any ectoparasiticidal compound that is soluble in a (C 3

-C

6 branched alkyl (C 10

-C

20 alkanoate vehicle and is useful for topical application can be incorporated as the insecticidal component of these formulations. Typically, the insecticidal agent is active against a broad spectrum of pest species, including acaricides, antiparasitic agents, insect growth regulators and compounds that inhibit or kill flies, flying pests and other "temporary" pests that only alight momentarily on domesticated animals.

Examples of useful classes of insecticides are spinosyns, organophosphates, organochlorines, carbamates, and pyrethrins. Specific useful insecticidal compounds 25 include tetraethyl pyrophosphate (TEPP), mevinphos, disulfoton, azinphosmethyl, parathion, methylparathion, chlorfenvinphos, cichlorvos, diazinon, dimethoate, o trichlorfon, chlorothion, malathion, ronnel, abate, baygon, carbaryl, mobam, temik, o zectran, methoxychlor, aldrin, dieldrin, endrin, heptachlor, S S O O [R:\LIBVV]03350speci.doc:njc WO 01/12156 PCT/US00/19549 -4chlordane, lindane, mirex, nicotine, rotenoids, pyrethrums, spinosyns and synthetic pyrethroids, including cypermethrin.

Preferred insecticides useful in these formulations are spinosyns or a pyrethroid such as cypermethrin.. Spinosyns are especially preferred.

The spinosyns (also known as A83453 factors) are agricultural insecticides that have shown activity against southern armyworm and other insects in the order Lepidoptera, and cotton aphid and other members of the order Homoptera.

(See, for example, U.S. Patent No. 5,571,901).

The spinosyns were also known to have some ectoparasiticidal activity, they had in vitro activity against mosquito larvae, black blowfly larvae and adult stable flies, which are members of the insect order Diptera, and transient systemic activity against larval blowfly and adult stable fly in guinea pigs and sheep. For these tests, the spinosyns were administered in aqueous polyvinylpyrrolidone or in polyethylene glycol (see U.S. Patent No. 5,571,901, col. 26-32).

The spinosyns are naturally-derived macrolides produced by fermentation of Saccharopolyspora spinosa. The fermentation produces multiple factors, including spinosyn A and spinosyn D (also called A83543A and A8354D).

Spinosyn A and spinosyn D are the two spinosyns that are most active as insecticides.

An agricultural product comprised mainly of these two spinosyns is available commercially under the name "spinosad".

Spinosyn A was the first spinosyn isolated and identified from the fermentation broth of Saccharopolyspora spinosa. Subsequent examination of the fermentation broth revealed that S. spinosa produced a number of spinosyns that have been called spinosyns A to H and J. Additional spinosyns, denominated K to W, have been identified from various strains ofS. spinosa. The various spinosyns are characterized by differences in the substitution patterns on the amino group of the forosamine, at selected sites on the tetracyclic ring system and on the 2N,3N,4N-(tri- O-methyl)rhamnose group.

Boeck et al. described spinosyns A-H and J (which they called A83543 factors A, B, C, D, E, F, G, H and and salts thereof, in U.S. Patent Nos. 5,362,634 (issued Nov. 8, 1994); 5,496,932 (issued March 5, 1996); and 5,571,901 (issued Nov. 5, 1996). Mynderse et al. described spinosyns L-N (which they called A83543 WO 01/12156 PCT/US00/19549 factors L, M and their N-dimethyl derivatives, and salts thereof, in U.S. Patent No. 5,202,242 (issued Apr. 13, 1993); and Turner et al. described spinosyns Q-T (which they called A83543 factors Q, R, S and their N-dimethyl derivatives, and salts thereof, in U.S. Patent Nos. 5,591,606 (issued January 7, 1997) and 5,631,155 (issued May 29, 1997). Spinosyns K, O, P, U, V, W and Y are described, for example, by Carl V. DeAmicis, James E. Dripps, Chris J. Hatton and Laura I. Karr in American Chemical Society's Symposium Series: Phytochemicals for Pest Control, Chapter 11, "Physical and Biological Properties of Spinosyns: Novel Macrolide Pest- Control Agents from Fermentation", pages 146-154 (1997).

The spinosyns can be isolated in the form of salts that are also useful in the formulations of this invention. The salts are prepared using standard procedures for salt preparation. For example, spinosyn A can be neutralized with an appropriate acid to form an acid addition salt. Representative suitable acid addition salts include salts formed by reaction with either an organic or inorganic acid, for example, sulfuric, hydrochloric, phosphoric, acetic, succinic, citric, lactic, maleic, fumaric, cholic, pamoic, mucic, glutamic, camphoric, glutaric, glycolic, phthalic, tartaric, formic, lauric, stearic, salicylic, methanesulfonic, benzenesulfonic, sorbic, picric, benzoic, cinnamic and like acids.

The term a "spinosyn or a derivative thereof' as used herein refers to an individual spinosyn factor (spinosyn A, B, C, D, E, F, G, H, J, K, L, M, N, O, P, Q, R, S, T, U, V, W or an N-dimethyl derivative of one or more spinosyn factor, or a combination thereof. For convenience, the term "spinosyn component" as used means an individual spinosyn or a physiologically acceptable derivative or salt thereof, or a combination thereof. "Spinosad" as used herein refers to a mixture of spinosyns comprised mainly of spinosyns A and D.

The spinosyns are known to have excellent human and animal safety and toxicological profiles. Because of their low toxicity to animals and humans, spinosyns are considered to be environment-friendly, "green" insecticides. It is desirable to formulate spinosyns to maintain this "green" profile.

Spinosyns have recently been found to be useful in the eradication or control ofectoparasites on sheep and companion animals. Thus, formulations of WO 01/12156 PCT/US00/19549 -6spinosyns with low toxicity and increased stability are potentially valuable in combating ectoparasites and the diseases such pests often carry.

The present formulations further comprise a (C 3

-C

6 branched alkyl (Co-C 20 alkanoate. This component is an organic solvent that acts as a spreading agent. Spreading agents increase the spreading of, and aid in substantially equalizing the distribution of, the active ingredient over the hair and/or skin surface area of the animal over time. The spreading agent solvent system should be safe, non-toxic, environment-friendly and non-flammable.

The branched alkyl portion of the (C 3

-C

6 branched alkyl (C 0 o-C 20 alkanoate includes all branched chain isomers of C 3 -C alkyl groups. Examples are isopropyl, isobutyl, isopentyl, and isohexyl. The (Cit-C 20 alkanoate moiety includes all C 10 -C2o fatty alkanoate groups, including but not limited to, decanoate (CIo), hendecanoate (CII), dodecanoate (CI 2 tridecanoate tetradecanoate (C 14 pentadecanoate (CS1), hexadecarioate (Cs 1 heptadecanoate (C17), octadecanoate (Cig), and eicosanoate (C 2 0 Preferably, the spreading agent is a (C 3

-C

6 branched alkyl

(C

12 -Cia) alkanoate. Of these solvents, C 3 branched alkyl-Ci4 alkanoates are especially useful. A preferred spreading agent is isopropyl myristate (IPM).

The formulations can optionally contain a miscibilizing agent. The miscibilizing agent aids in solubilizing the active ingredient and must be compatible with organic solvent systems. The phrase "compatible with organic solvent systems" means that the miscibilizing agent does not form more than one phase when mixed with the (C 3

-C

6 branched alkyl (CIo-C 2 0 alkanoate component.

Suitable miscibilizing agents for use in these formulations generally are (C,-C 30 organic acids. Typically, such organic acids are straight-chain saturated fatty acids, but they can also be low molecular weight organic acids such as formic acid, acetic acid, propionic acid and benzoic acid.

The choice of miscibilizing agent will vary depending on the insecticide in the formulation. When the insecticide component is a spinosyn, examples of suitable miscibilizing agents are formic acid, acetic acid, propionic acid, butyric acid, valeric acid, caproic acid, benzoic acid, enanthic acid, caprylic acid, pelargonic acid, capric acid, undecylic acid, lauric acid, tridecylic acid, myristic acid, pentadecylic acid, palmitic acid, margaric acid, stearic acid, oleic, arachidic acid, behenic acid, WO 01/12156 PCT/US00/19549 -7lignoceric acid, cerotic acid, montanic acid, triacontanoic acid, psyllic acid, and ceroplastic acid. Other useful miscibilizing agents are (C,-C 3 0 alkyl sulfuric acids,

(C,-C

30 alkyl phosphoric acids, and (C,-C 30 alkyl sulfonic acids.

In formulations containing a spinosyn solubilized in isopropyl myristate, for example, oleic acid is a particularly useful miscibilizing agent because it aids in solubilizing the spinosyn (thus allowing for the formation of solvent solutions containing relatively high concentrations of active ingredient), and it is compatible with the isopropyl myristate component.

Compounds other than (Ci-Co 3 organic acids can also be useful miscibilizing agents in the formulations of this invention. In general, a miscibilizing compound useful for these formulations: 1) is compatible with the selected organic solvent component, and 2) solubilizes the active ingredient without substantially altering the spreading properties of the formulation.

When insecticide is a spinosyn and the spinosyn component is spinosyn D or spinosad a mixture of spinosyns A and it is especially important that the miscibilizing agent is able to solubilize the spinosyn D sufficiently.

In technical grade spinosad, factor D is generally the factor that causes solubility problems when preparing spinosad-containing formulations. Examples of miscibilizing agents that are useful for spinosad-containing ectoparasiticidal formulations of the present invention include, but are not limited to, benzyl alcohol, ethylene glycol phenyl ether, D-limonene, N-methyl-2-pyrrolidinone, and methylated soybean oils and soybean oil methyl esters, such as SOYGOLD 1000 (AG Environmental Products LLC).

The present formulations can also contain other optional ingredients, such as: antioxidants, UV-absorbing compounds or photostabilizers, viscositymodifying agents, antimicrobial agents, dyes, perfumes, deodorants and physiologically or dermatologically acceptable carriers, diluents, excipients or adjuvants. Such agents are known in the art.

For example, one or more antioxidants can be added to the formulations in an amount effective to retard oxidation of the formulation components and the ensuing degradative effects. Potentially useful antioxidants include primary WO 01/12156 PCT/US00/19549 -8antioxidants that are radical scavengers, such as hindered phenolics and secondary amines, and secondary antioxidants, such as phosphites and thioesters that function as peroxide decomposers. Preferred antioxidants for use in these formulations are blends of primary and secondary antioxidants, including particularly blends of phenolic and phosphite antioxidant compositions.

There are many commercially available antioxidants products designed for polymer stabilization, including antioxidant formulations comprising synergistic combinations of primary and secondary antioxidants. Examples of commercially available antioxidants useful in the formulations of this invention include the Irganox® antioxidants available from Ciba Geigy, Vanox® antioxidants from R.T.

Vanderbilt, and the Naugard® antioxidants available from Uniroyal Chemicals.

When the formulations include an antimicrobial component, it should be present in an amount effective to prevent the growth of microorganisms in the formulation.

Generally, the formulations of this invention can be prepared by blending the components with adequate mixing or stirring. For example, a useful spinosad formulation is one having a final concentration of 2 mg of spinosad per mL.

One such formulation is prepared to contain 99.1 weight percent IPM, 0.6 weight percent oleic acid, and 0.3 weight percent spinosad technical (89% active ingredient).

This formulation is made by adding the appropriate amount of spinosad to the IPM solvent with mixing or stirring, blending the oleic acid into the IPM/spinosad mixture, and continuing the mixing or stirring until the spinosad has completely solubilized to form the final formulation product. An optional additional step is to filter the final formulation to remove any impurities or extraneous materials.

The formulations of this invention are applied to the animal topically.

Topical control protocols include spot-on or pour-on treatments wherein the formulation is placed directly onto a discreet skin and/or hair surface area of the animal and allowed to spread over the remainder of the animal's skin or hair surface area. Generally, spot-on or pour-on protocols involve initially placing the formulation on the dorsal midline the head, neck, shoulders or back) of the animal.

Placement typically occurs on a dorsal midline surface area that constitutes less than of the animal's entire surface area. For example, a typical pour-on treatment WO 01/12156 PCT/US00/19549 -9protocol involves applying about 4 to about 50 mL of a liquid ectoparasiticidal formulation in a narrow strip along the backline of an animal, from the withers to the tail or rump.

For spot-on or pour-on treatment to control ectoparasites, such as lice, which are present over the whole surface of an animal, the ectoparasiticidal active ingredient must spread from the narrow strip at the backline to cover the entire surface of the animal. The present formulations have this advantageous spreading effect. Of course, they can be applied to areas of skin that constitute greater than 10% of the surface area of the animal, but such applications limit the advantage offered by these formulations. Another advantage of these formulations is that they offer extended ectoparasiticidal coverage and need not be applied more than weekly or biweekly at most.

Example 1 Wetting Tests for Various Solvent Systems To determine organic solvents that are useful spreading agents, solvent systems that are capable of solubilizing at least 1% spinosad by weight percent were screened for hair wetting by applying about 1 mL of the solvent system (solvents were screened without active ingredient), dropwise, to a tanned rabbit pelt that was at an angle of about 45 Solvent systems that wet the rabbit hair and did not run off before wetting the hair were considered to pass the screen. Table I describes the ability of selected organic solvents to wet the rabbit hair.

WO 01/12156 PCT/US00/19549 Table I. Ability of Solvents and Aqueous Surfactant Systems to Wet Rabbit Hair on Tanned Rabbit Pelts.

Organic Solvent Wet Well isopropyl myristate methyl laurate dipropylene glycol methyl ether butyl lactate methyl caprate methyl oleate octanoic acid limonene hexanol Did Not Wet triacetin N-methyl pyrrolidone propylene glycol ethyl oleate As Table I shows, water immiscible, nonpolar solvents generally wet well, although dipropylene glycol methyl ether is water miscible and did wet the hair very well.

Example 2 Formulation Spreading Tests Further studies were conducted to determine the abilities of various solvents to aid in spreading of active ingredient.

International Patent Application WO 9524219 teaches that wool grease fraction (Fl) provides superior spreading ofpyrethroid insecticides on sheep when compared to organic solvent spreading agents. Tests were conducted to compare the spreading properties of formulations containing the Fl wool grease fraction and various organic solvents. The amount and rate of diffusion of 1 4 C-labeled zetacypermethrin from the dorsal midline of sheep were determined when applied in Fl wool grease fraction and a range of test excipients. Four formulations containing WO 01/12156 PCT/US00/19549 -11mg/mL zeta-cypermethrin spiked with 100 gCi 4 C] zeta-cypermethrin were prepared in the following vehicles: wool grease fraction Fl, isopropyl myristate, octyl stearate and glyceryl tricaprylate/caprate. A dose of 1 mL/5kg body weight of each formulation was applied to the backline of 3 sheep. Wool was collected and pooled 1, 2, 4, 8, 11 and 14 days after treatment from three 12 x 12 mm squares chosen at random, along meridian lines drawn 2, 7.5 and 15 cm down the side of each sheep from the backline. The clipped areas were also swabbed. At day 14 after treatment the wool at the site of application was collected, and back and perirenal fat samples were collected. The quantity of zeta-cypermethrin in each sample was measured by liquid scintillation counting.

For the majority of the measurements taken, isopropyl myristate gave the greatest spread of zeta-cypermethrin and the wool grease fraction Fl provided the least spreading. When the vehicle was the Fl wool grease fraction, only the 2-cm meridian showed increased concentration of zeta-cypermethrin over time after initial application. When the vehicle was isopropyl myristate, the quantity of zetacypermethrin measured at all meridians increased with time following administration.

Octyl stearate and glyceryl tricaprylate/caprate gave modest spread, but not as great as the spread provided by the IPM formulations. Tissue residues were similar amongst formulations except the glyceryl tricaprylate/caprate formulation appeared to cause the highest residue levels.

After two weeks it was determined that Fl was a comparatively poor spreading agent, octyl stearate and glyceryl tricaprylate/caprate provided better spreading properties, and isopropyl myristate gave the best spread of zetacypermethrin.

Example 3 Ectoparasiticidal Efficacy Tests of Wool Grease and IPM Formulations Tests were conducted to compare the efficacy of formulations of spinosad in Fl wool grease fraction versus those in isopropyl myristate against lice on sheep. Two Fl formulations containing spinosad at 2 mg/mL and 10 mg/mL, and one formulation containing 2 mg/mL spinosad in isopropyl myristate were made as follows: WO 01/12156 PCT/US00/19549 -12a) Fl Formulation (2 mg/mL) 81.11 F 18.62 solvent (50/50 petroleum ether/acetone) 0.27 spinosad technical that was 89% active 100 (w/w) Formulation density 0.84 g/mL b) FI Formulation (10 mg/mL) 80.26 Fl 18.42 solvent (50/50 petroleum ether/acetone) 1.32 spinosad technical that was 89% active 100% Formulation density 0.84 g/mL c) IPM Formulation (2 mg/mL) 99.123 IPM 0.613 oleic acid 0.264 spinosad technical that was 89% active 100% Formulation density 0.85 g/mL Each formulation was administered to sheep as a pour-on application immediately after shearing. Spinosad/FI formulations were tested at doses of0 vehicle alone), 0.4 and 2 mg/kg, while the spinosad/IPM formulation was tested at a Jose of 0.4 mg/kg. The 2 mg/kg dose o spinosad/F1 was administered using the mg/mL formulation, while the 0.4 mg/kg doses were administered using the 2 mg/mL formulations. Lice counts were taken on the sheep at sites all over the animal, including the head and neck. These lice counts were taken before treatment and on or about weekly for the following eight weeks. The results of this study are summarized in Table II.

WO 01/12156 PCT/US00/19549 -13- Table II: Comparison of Ectoparasiticidal Efficacy of Spinosad in Fl and IPM Formulations in Sheep Lice Counts (mean) Week of Study DavO 1 2 3 4 5 6 7 8 lice count FI Only 643 428 328 350 237 245 159 156 145 0.4 mg/kg 622 391 213 211 159 118 103 69 68 Spinosad/F 2 mg/kg 582 146 86 48 2 13 4 3 2 Spinosad/FI 0.4 mg/kg 575 138 81 68 47 32 13 25 21 Spinosad/IPM As the results summarized in Table II show, spinosad in IPM at a dose of 0.4 mg/kg was superior in lice control to spinosad in Fl at 0.4 mg/kg, and was almost equal in efficacy to spinosad in Fl at 2 mg/kg. Spinosad in IPM gave outstanding control of lice on sheep, including control of lice on the head and neck, which indicated that IPM potentiated the spreading of spinosad from the dorsal midline to the head, neck and other body surface regions.

Example 4 Efficacy of Spinosad in Various Organic Solvents vs. Lice in Sheep Further lice efficacy studies on sheep were conducted to compare the efficacy of PM as a spreading agent to that of other organic solvent systems containing blends of organic solvents. The formulations tested had the following compositions: a) IPM Formulation: 99.12 IPM 0.61 oleic acid 0.27 spinosad technical (89% active) WO 01/12156 PCT/USOO/19549 -14b) OP/IPM Formulation 79.78% octyl palmitate (OP) 19.95% IPM 0.27% spinosad technical (89% active) c) GTCC/OP Formulation 79.78% glyceryl tricaprylate/caprate (GTCC) 19.95% octyl palmitate 0.27% spinosad technical (89% active) d) GTCC/IPM/CAP Formulation 69.81% glyceryl tricaprylate/caprate 14.96% IPM 14.96% cetearyl octanoate (CAP) 0.27% spinosad technical (89% active) e) OP/IPM/OSU Formulation 69.81% octyl palmitate 14.96% IPM 14.96% dioctyl succinate (OSU) 0.27% spinosad technical (89% active) f) TPM/LWG/GTCC Formulation 59.84% tripropylene glycol methyl ether (TPM) 19.95% liquid wool grease (LWG) 19.95% glyceryl tricaprylate/caprate 0.27% spinosad technical (89% active) g) TPM/OSU Formulation 79.78% tripropylene glycol methyl ether 19.95% dioctyl succinate 0.27% spinosad technical (89% active) Lice counts were taken on the sheep at sites all over the animal, including the head and neck. These lice counts were taken before treatment and on or about weekly for the following twelve weeks. The results of this study summarized in Table III.

WO 01/12156 PCT/US00/19549 Table III: Efficiacy of Spinosad in Organic Solvent Formulations vs. Lice in Sheep Lice Count (mean) Weeks Treatment Pretreatment 1 2 4 6 8 12 lice count Control 253 197 128 163 206 162 204 IPM 268 33 23 18 20 18 18 OP/IPM 271 71 51 83 90 71 184 GTCC/OP 257 32 28 40 52 46 106 GTCC/IPM/CAP 278 28 16 24 25 22 33 OP/TPM/OSU 259 33 19 23 22 19 TM/LWG/GTCC 267 53 29 39 34 27 48 TPM/OSU 259 58 38 64 62 66 119 As Table III shows, spinosad in IPM alone was the most effective treatment of the 7 formulations tested. A strong correlation exists between the results of the spreading experiments and those of the field efficacy experiments. Spinosad formulations in IPM exhibited excellent spreading characteristics and demonstrated outstanding long-term protective and inhibitory effects against ectoparasiticidal infestation in sheep.

Examples 5-11 illustrate various formulations of this invention.

Example 5: Spinosad/IPM/acetic acid Formulation 5.65% spinosad (88.5% active) 3% acetic acid 91.35% IPM Example 6: Spinosad/IPM/octanoic acid Formulation 5.65% spinosad (88.5% active) 7.5% octanoic acid 86.85% IPM WO 01/12156 WO 0112156PCT[USOO/19549 -16- Example 7: Spinosad/IPM]lauric acid Formulation 5.65% spinosad (88.5% active) 15% lauric acid 84.2% IPM Example 8: Spinosad/IPMlolei'c acid Formulation 5.65% spinosad (88.5% active) 16.5% oleic acid 77.85% 1PM Example 9: Spinosad/lPM/benzoic acid Formnulation 5.65% spinosad (88.5% active) 3.76% benzoic acid 90.59% 1PM Example 10: Spinosad/IPM/NMP Formulation 5.65% spinosad (88.5% active) 40.0% 1 -methyl-2-pyrrolidinone (NMP) 54.35% 1PM Example 11: Zeta-cvpermnethrin/IPM Formulation 1. 18% zeta-cypermethrin (84.7% active) 98.82% 1PM The formulations of Examples 5-10 can be prepared by weighing the spinosad into a suitable container, adding the 1PM and stirring to create a slurry, and then adding the final component and stirring until a clear solution is achieved. In preparing the formulation of Example 11, the zeta-cypermethrin is an oily liquid that -equires gentle heating (approximately 4-)-5 0 C) to allow for proper mixing into. the organic,solvent phase. No separation of phases is evident upon cooling.

Claims (8)

1. A topical ectoparasiticidal formulation comprising from 0.1 to 25 weight percent of a spinosyn, or a physiologically acceptable derivative or salt thereof; from to 99 weight percent of isopropyl myristate and from 0.6 to 40 weight percent of a miscibilizing agent compatible with organic solvent systems, selected from acetic, lauric, octanoic, oleic and benzoic acids and N-methyl-2-pyrrolidinone.

2. A formulation of Claim 1 wherein the miscibilizing agent is oleic acid.

3. A topical ectoparasiticidal formulation substantially as herein described with reference to any one of Examples 1 to 11 but excluding any comparative examples 0o therein.

4. An article of manufacture, comprising packaging material and contained within said packaging material a topical formulation for controlling an ectoparasite infestation on a small ruminant or companion animal, wherein said formulation comprises a topical unit dose of a formulation of any one of Claims 1 to 3; and, wherein said 15 packaging material comprises a label or package insert with instructions for topically administering the dose to the animal.

5. A topical ectoparasiticidal formulation comprising from 0.1 to 25 weight percent of a spinosyn, or a physiologically acceptable derivative or salt thereof; from to 99 weight percent of isopropyl myristate and from 0.6 to 40 weight percent of a miscibilizing agent compatible with organic solvent systems, selected from acetic, lauric, octanoic, oleic and benzoic acids and N-methyl-2-pyrrolidinone when used to control an ectoparasite infestation on a small ruminant or companion animal.

6. A topical ectoparasiticidal formulation comprising from 0.1 to 25 weight percent of a spinosyn, or a physiologically acceptable derivative or salt thereof; from 25 to 99 weight percent of isopropyl myristate and from 0.6 to 40 weight percent of a miscibilizing agent compatible with organic solvent systems, selected from acetic, lauric, octanoic, oleic and benzoic acids and N-methyl-2-pyrrolidinone for use in controlling an ectoparasite infestation on a small ruminant or companion animal.

7. A method of controlling an ectoparasite infestation on a small ruminant or companion animal, comprising topically applying to the animal a formulation of any one of Claims 1 to 3 or 5 and 6.

8. Use of from 0.1 to 25 weight percent of a spinosyn, or a physiologically acceptable derivative or salt thereof in combination with from 25 to 99 weight percent of isopropyl myristate in combination with from 0.6 to 40 weight percent of a miscibilizing agent compatible with organic solvent systems, selected from acetic, lauric, octanoic, [R:\LIBC]43208.doc:ais [I:\DayLib\LIBC]43208.doc:ais 18 oleic and benzoic acids and N-methyl-2-pyrrolidinone for the manufacture of a medicament to control an ectoparasite infestation on a small ruminant or companion animal. Dated 11 February, 2002 Eli Lilly and Company Patent Attorneys for the Applicant/Nominated Person SPRUSON FERGUSON [R:\LI BC]43208.doc ais [I:\DayLib\LIBC]43208.doc:ais