AU742613B2 - Neocartilage and methods of use - Google Patents
Neocartilage and methods of use Download PDFInfo
- Publication number
- AU742613B2 AU742613B2 AU71003/98A AU7100398A AU742613B2 AU 742613 B2 AU742613 B2 AU 742613B2 AU 71003/98 A AU71003/98 A AU 71003/98A AU 7100398 A AU7100398 A AU 7100398A AU 742613 B2 AU742613 B2 AU 742613B2
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- AU
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- Prior art keywords
- neocartilage
- chondrocytes
- cartilage
- biological material
- growth media
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8945535B2 (en) | 2005-09-20 | 2015-02-03 | Zimmer Orthobiologics, Inc. | Implant for the repair of a cartilage defect and method for manufacturing the implant |
Families Citing this family (60)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4394169B2 (ja) * | 1997-04-04 | 2010-01-06 | バーンズ − ジューウィッシュ・ホスピタル | 新軟骨および使用方法 |
| AU7984498A (en) * | 1997-06-25 | 1999-01-04 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Serum-free cell growth medium |
| US6528052B1 (en) | 2000-09-29 | 2003-03-04 | The Board Of Trustees Of The Leland Stanford Junior University | Method for in vivo ex vivo and in vitro repair and regeneration of cartilage and collagen and bone remodeling |
| EP1099443A1 (en) | 1999-11-11 | 2001-05-16 | Sulzer Orthopedics Ltd. | Transplant/implant device and method for its production |
| ATE414141T1 (de) | 2000-08-09 | 2008-11-15 | Hiroko Yanaga | Verfahren zur kultivierung humaner chondrozyten |
| ITRM20010088A1 (it) * | 2001-02-21 | 2002-08-21 | Idi Irccs | Peptide in grado di inibire l'attivita' del fattore di crescita derivato dalle piastrine (pdgf-bb) e del fattore di crescita derivato dai fi |
| WO2002076285A2 (en) | 2001-03-23 | 2002-10-03 | Histogenics Corporation | Composition and methods fro the production of biological tissues and tissue constructs |
| US20040151705A1 (en) | 2002-03-22 | 2004-08-05 | Shuichi Mizuno | Neo-cartilage constructs and a method for preparation thereof |
| WO2003061653A1 (en) * | 2002-01-22 | 2003-07-31 | Pfizer Limited | 3-(imidazolyl)-2-aminopropanoic acids for use as tafi-a inhibitors for the treatment of thrombotic diseases |
| US7537780B2 (en) | 2002-03-22 | 2009-05-26 | Histogenics Corporation | Method for preparing and implanting a cartilage construct to treat cartilage lesions |
| US7468192B2 (en) | 2002-03-22 | 2008-12-23 | Histogenics Corporation | Method for repair of cartilage lesions |
| US7622562B2 (en) | 2002-06-26 | 2009-11-24 | Zimmer Orthobiologics, Inc. | Rapid isolation of osteoinductive protein mixtures from mammalian bone tissue |
| US20040153145A1 (en) * | 2002-11-26 | 2004-08-05 | Clemson University | Fixation method for bioprostheses |
| US7189259B2 (en) * | 2002-11-26 | 2007-03-13 | Clemson University | Tissue material and process for bioprosthesis |
| US8173162B2 (en) * | 2003-02-26 | 2012-05-08 | Zimmer Orthobiologics, Inc. | Preparation for repairing cartilage tissue, especially articular cartilage defects |
| US7642092B2 (en) * | 2003-03-03 | 2010-01-05 | Technion Research & Development Foundation Ltd. | Cultured cartilage/bone cells/tissue, method of generating same and uses thereof |
| US7067123B2 (en) | 2003-04-29 | 2006-06-27 | Musculoskeletal Transplant Foundation | Glue for cartilage repair |
| US7901457B2 (en) | 2003-05-16 | 2011-03-08 | Musculoskeletal Transplant Foundation | Cartilage allograft plug |
| US7488348B2 (en) * | 2003-05-16 | 2009-02-10 | Musculoskeletal Transplant Foundation | Cartilage allograft plug |
| JP2007502127A (ja) * | 2003-05-23 | 2007-02-08 | ホイゼルマン・ハンス・イェルク | 生体外で軟骨様組織を生成させる方法 |
| US20050124038A1 (en) * | 2003-07-14 | 2005-06-09 | Pharmacia Corporation | Transfection of cartilage and matrices thereof in vitro |
| US7824711B2 (en) | 2003-12-11 | 2010-11-02 | Isto Technologies, Inc. | Particulate cartilage system |
| US20060275273A1 (en) * | 2004-02-20 | 2006-12-07 | Seyedin Mitchell S | Intervertebral Disc Repair, Methods and Devices Therefor |
| ATE553186T1 (de) * | 2004-02-20 | 2012-04-15 | Isto Technologies Inc | Bandscheibenreparatur und verfahren dafür |
| US8697139B2 (en) | 2004-09-21 | 2014-04-15 | Frank M. Phillips | Method of intervertebral disc treatment using articular chondrocyte cells |
| US8017394B2 (en) * | 2004-10-01 | 2011-09-13 | Isto Technologies, Inc. | Method for chondrocyte expansion with phenotype retention |
| US7273756B2 (en) * | 2004-10-01 | 2007-09-25 | Isto Technologies, Inc. | Method for chondrocyte expansion with phenotype retention |
| US7837740B2 (en) | 2007-01-24 | 2010-11-23 | Musculoskeletal Transplant Foundation | Two piece cancellous construct for cartilage repair |
| WO2006050213A2 (en) * | 2004-10-29 | 2006-05-11 | Michalow Alexander E | Methods of promoting healing of cartilage defects and method of causing stem cells to differentiate by the articular chondrocyte pathway |
| US7815926B2 (en) * | 2005-07-11 | 2010-10-19 | Musculoskeletal Transplant Foundation | Implant for articular cartilage repair |
| EP1916964A4 (en) * | 2005-08-26 | 2015-11-04 | Zimmer Inc | IMPLANTS AND METHODS FOR THE REPAIR, REPLACEMENT AND TREATMENT OF JOINT DISEASES |
| US8921109B2 (en) | 2005-09-19 | 2014-12-30 | Histogenics Corporation | Cell-support matrix having narrowly defined uniformly vertically and non-randomly organized porosity and pore density and a method for preparation thereof |
| EP2264149A1 (en) * | 2005-11-10 | 2010-12-22 | Carticure Ltd. | Method for non-autologous cartilage regeneration |
| US7416889B2 (en) * | 2006-04-27 | 2008-08-26 | Rhode Island Hospital | Methods and compositions for repairing cartilage |
| EP1894581A1 (en) * | 2006-08-31 | 2008-03-05 | CellCoTec B.V. | Repair of cartilage tissue using a matrix gel containing chondrocytes |
| US8142805B1 (en) | 2006-11-06 | 2012-03-27 | Clemson University Research Foundation | Implantable devices including fixed tissues |
| US8163549B2 (en) | 2006-12-20 | 2012-04-24 | Zimmer Orthobiologics, Inc. | Method of obtaining viable small tissue particles and use for tissue repair |
| US8435551B2 (en) | 2007-03-06 | 2013-05-07 | Musculoskeletal Transplant Foundation | Cancellous construct with support ring for repair of osteochondral defects |
| US8423148B1 (en) * | 2007-03-19 | 2013-04-16 | Michael Lytinas | Method for treating cartilage defects |
| AU2008240191B2 (en) | 2007-04-12 | 2013-09-19 | Zimmer, Inc. | Compositions and methods for tissue repair |
| US20090054984A1 (en) | 2007-08-20 | 2009-02-26 | Histogenics Corporation | Method For Use Of A Double-Structured Tissue Implant For Treatment Of Tissue Defects |
| US8685107B2 (en) | 2007-07-03 | 2014-04-01 | Histogenics Corporation | Double-structured tissue implant and a method for preparation and use thereof |
| EP2182887B1 (en) | 2007-08-20 | 2016-12-14 | Histogenics Corporation | A method for improvement of differentiation of mesenchymal stem cells using a double-structured tissue implant |
| CA2717725A1 (en) * | 2008-03-05 | 2009-09-11 | Musculoskeletal Transplant Foundation | Cancellous constructs, cartilage particles and combinations of cancellous constructs and cartilage particles |
| US9192695B2 (en) | 2008-11-20 | 2015-11-24 | Allosource | Allografts combined with tissue derived stem cells for bone healing |
| US10130736B1 (en) | 2010-05-14 | 2018-11-20 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| US8883210B1 (en) | 2010-05-14 | 2014-11-11 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| US9352003B1 (en) | 2010-05-14 | 2016-05-31 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| US8834928B1 (en) | 2011-05-16 | 2014-09-16 | Musculoskeletal Transplant Foundation | Tissue-derived tissugenic implants, and methods of fabricating and using same |
| US20140017283A1 (en) | 2012-07-11 | 2014-01-16 | Osiris Therapeutics, Inc. | Porated cartilage products |
| US9186380B2 (en) | 2012-11-15 | 2015-11-17 | Allosource | Minced cartilage systems and methods |
| US20140178343A1 (en) | 2012-12-21 | 2014-06-26 | Jian Q. Yao | Supports and methods for promoting integration of cartilage tissue explants |
| US9186253B2 (en) | 2013-02-22 | 2015-11-17 | Allosource | Cartilage mosaic compositions and methods |
| AU2014236705B2 (en) | 2013-03-15 | 2018-04-12 | Allosource | Perforated osteochondral allograft compositions |
| KR102312720B1 (ko) | 2013-03-15 | 2021-10-13 | 알로소스 | 연조직 회복 및 재생을 위한 세포 재배치된 콜라겐 매트릭스 |
| EP4509147A2 (en) | 2014-08-01 | 2025-02-19 | Isto Technologies, Inc. | Cartilage compositions and methods for modifying proteoglycan content |
| US10077420B2 (en) | 2014-12-02 | 2018-09-18 | Histogenics Corporation | Cell and tissue culture container |
| EP3297694A1 (en) | 2015-05-21 | 2018-03-28 | Musculoskeletal Transplant Foundation | Modified demineralized cortical bone fibers |
| US11696929B2 (en) | 2017-09-20 | 2023-07-11 | The Regents Of The University Of California | Methods and systems for conserving highly expanded cells |
| JP7113436B2 (ja) * | 2020-02-17 | 2022-08-05 | 義昭 工藤 | コンドロイチン硫酸型プロテオグリカン及びヒアルロン酸の製造方法 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL68218A (en) | 1983-03-23 | 1985-12-31 | Univ Ramot | Compositions for cartilage repair comprising embryonal chondrocytes |
| US4681763A (en) * | 1985-06-11 | 1987-07-21 | University Of Medicine And Dentistry Of New Jersey | Composition for stimulating bone growth |
| US5902741A (en) | 1986-04-18 | 1999-05-11 | Advanced Tissue Sciences, Inc. | Three-dimensional cartilage cultures |
| US4877864A (en) * | 1987-03-26 | 1989-10-31 | Genetics Institute, Inc. | Osteoinductive factors |
| CA1340581C (en) | 1986-11-20 | 1999-06-08 | Joseph P. Vacanti | Chimeric neomorphogenesis of organs by controlled cellular implantation using artificial matrices |
| US5041138A (en) | 1986-11-20 | 1991-08-20 | Massachusetts Institute Of Technology | Neomorphogenesis of cartilage in vivo from cell culture |
| US5736372A (en) | 1986-11-20 | 1998-04-07 | Massachusetts Institute Of Technology | Biodegradable synthetic polymeric fibrous matrix containing chondrocyte for in vivo production of a cartilaginous structure |
| US4846835A (en) | 1987-06-15 | 1989-07-11 | Grande Daniel A | Technique for healing lesions in cartilage |
| US5688678A (en) | 1990-05-16 | 1997-11-18 | Genetics Institute, Inc. | DNA encoding and methods for producing BMP-8 proteins |
| US5326357A (en) | 1992-03-18 | 1994-07-05 | Mount Sinai Hospital Corporation | Reconstituted cartridge tissue |
| WO1994009118A1 (en) * | 1992-10-13 | 1994-04-28 | The General Hospital Corporation | Immortalized human chondrocytes |
| US5709854A (en) | 1993-04-30 | 1998-01-20 | Massachusetts Institute Of Technology | Tissue formation by injecting a cell-polymeric solution that gels in vivo |
| US5368051A (en) | 1993-06-30 | 1994-11-29 | Dunn; Allan R. | Method of regenerating articular cartilage |
| US5585237A (en) * | 1993-10-25 | 1996-12-17 | Creative Biomolecules, Inc. | Methods and compositions for high protein production from recombinant DNA |
| DK0733109T3 (da) | 1993-12-07 | 2006-07-03 | Genetics Inst Llc | BMP-12, BMP-13 og seneinducerende præparater dermed |
| US5723331A (en) | 1994-05-05 | 1998-03-03 | Genzyme Corporation | Methods and compositions for the repair of articular cartilage defects in mammals |
| US5906827A (en) * | 1994-06-03 | 1999-05-25 | Creative Biomolecules, Inc. | Matrix for the manufacture of autogenous replacement body parts |
| US5904716A (en) * | 1995-04-26 | 1999-05-18 | Gendler; El | Method for reconstituting cartilage tissue using demineralized bone and product thereof |
| WO1998004681A2 (en) * | 1996-07-25 | 1998-02-05 | Genzyme Corporation | Chondrocyte media formulations and culture procedures |
| JP4394169B2 (ja) * | 1997-04-04 | 2010-01-06 | バーンズ − ジューウィッシュ・ホスピタル | 新軟骨および使用方法 |
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1998
- 1998-04-03 JP JP54291298A patent/JP4394169B2/ja not_active Expired - Fee Related
- 1998-04-03 US US09/054,913 patent/US6235316B1/en not_active Expired - Lifetime
- 1998-04-03 CA CA002285382A patent/CA2285382C/en not_active Expired - Lifetime
- 1998-04-03 AU AU71003/98A patent/AU742613B2/en not_active Expired
- 1998-04-03 EP EP98917986.6A patent/EP1018987B1/en not_active Expired - Lifetime
- 1998-04-03 WO PCT/US1998/006586 patent/WO1998044874A1/en not_active Ceased
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1999
- 1999-12-21 US US09/467,520 patent/US6645764B1/en not_active Expired - Lifetime
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8945535B2 (en) | 2005-09-20 | 2015-02-03 | Zimmer Orthobiologics, Inc. | Implant for the repair of a cartilage defect and method for manufacturing the implant |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2285382C (en) | 2008-03-25 |
| US6235316B1 (en) | 2001-05-22 |
| EP1018987A1 (en) | 2000-07-19 |
| WO1998044874A9 (en) | 1999-04-01 |
| AU7100398A (en) | 1998-10-30 |
| WO1998044874A1 (en) | 1998-10-15 |
| US6645764B1 (en) | 2003-11-11 |
| US20030224518A1 (en) | 2003-12-04 |
| JP4394169B2 (ja) | 2010-01-06 |
| EP1018987B1 (en) | 2014-10-29 |
| CA2285382A1 (en) | 1998-10-15 |
| EP1018987A4 (en) | 2004-12-15 |
| US7087227B2 (en) | 2006-08-08 |
| JP2001519700A (ja) | 2001-10-23 |
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