AU663345B2 - Use of ionophoretic polyether antibiotics for controlling bacterial growth in industrial alcoholic fermentation - Google Patents
Use of ionophoretic polyether antibiotics for controlling bacterial growth in industrial alcoholic fermentation Download PDFInfo
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- AU663345B2 AU663345B2 AU29477/92A AU2947792A AU663345B2 AU 663345 B2 AU663345 B2 AU 663345B2 AU 29477/92 A AU29477/92 A AU 29477/92A AU 2947792 A AU2947792 A AU 2947792A AU 663345 B2 AU663345 B2 AU 663345B2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
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Abstract
The use of any ionophoretic polyether antibiotic in industrial alcoholic fermentation media is described. 0.5-1.5 p.p.m. of such antibiotics prevent bacterial growth and enhance the productivity of alcoholic fermentation using yeast.
Description
OPI. DATE, 15/06/93 AOJP DATE 19/08/93 APPLN. ID 29477/92 Illf I l111fllIlUIl1JI Il PCT NUMBER PCT/.FR92/00984 AU9229477 (51) Classification internationale des brevets 5 Numno de publication internationale: WO 93/10213 C12N 1/16, C12P 7/06 Al (43) Date de publication internationale: 27 mai 1993 (27.05.93) (21) Numnro de la demande internationale: PCT/FR92/00984 (22) Date de depbt international 20 octobre 1992 (20.10.92) Donn~es relatives A la priorit6: 91/14176 18 novembre 1991 (18.11.91) FR (71) D~posant (pour tous les Etats d~sign~s sauf US): UNION NATIONALE DES GROUPEMENTS DE DISTILLA- TEURS D'ALCOOL [FR/FR]; Laboratoire, 10, rue Barbette, F-75003 Paris (FR).
(72) Inventeur; et Inventeur/D~posant (US seulement) DE MINIAC, Michel [FR/FR]; 118, rue de Javel, F-75015 Paris (FR).
(74) Mandataire: UNION NATIONALE DES GROUPE- MENTS DE DISTILLATEURS D'ALCOOL Laboratoire, 10, rue Barbette, F-75003 Paris (FR).
(81) Etats d~sign~s: AT, AU, BB, BG, BR, CA, CH, CS, DE, DK, ES, Fl, GB, RU, JP, KP, KR, LK, LU, MG, MN, MW, NL, NO, PL, RO, RU, SD, SE, UA, US, brevet europ~en (AT, BE, CR, DE, DK. ES, FR, GB, GR, LE, IT, LU, MC, NL, SE), brevet OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, SN, TD, TG).
Publi~e Ai'ec rapport de rechzerchze internationale.
'0 (54) Title: USE OF TONOPHORETIC POLYETRER ANTIBIOTICS FOR CONTROLLING BACTERIAL GROWTH IN INDUSTRIAL ALCOROLIC FERMENTATION (54)Titre: UTILISATION DES ANTIBIOTIQUES IONOPRORES POLYETRERS POUR LIMITER LA CROISSANCE BACTERIENNE EN FERMENTATION ALCOOLIQUE INDUSTRIELLE (57) Abstract The use of any ionophoretic polyether antibiotic in industrial alcoholic fermentation media is described. 0.5-1.5 p.p.m. of such antibiotics prevent bacterial growth and enhance the productivity of alcoholic fermentation using yeast.
(57) Abr~g6 La pr~sente invention concerne l'utilisation d'un quelconque des antibiotiques ionophores poly~thers dans les milieux de fermentation alcoolique industrielle. Aux doses de 0,5 A 1,5 ces antibiotiques empkchent la croissance bact~rienne et permettent une meilleure productivit& de la fermentation alcoolique par la levure.
11 Ft WO 93/10213 PCT/FR92/00984 Use of polyether io-ophore antibiotics for limiting bacterial growth in industrial alcoholic fermentation.
The present invention relates to the use of any one of the polyether ionophore antibiotics for limiting bacterial growth in the following industrial alcoholic fermentation media: sugar-beet or -cane juice, sugar-beet or -cane molasses, hydrolysates of cereals or of tubers, wine- or cider-making products.
In the present state in which plants operate, the bacterial flora rises from 104 microbes/ml to 106 microbes/ml during the process of alcoholic fermentation.
At this concentration of 106 microbes/ml, there is a production of organic acids which interfere with the growth and the alcoholic fermentation of the yeast. This bacterial flora of lactic origin can also convert glycerol to acrolein in media of wine- or cider-making origin. Acrolein is a carcinogenic substance which subsequently finds its way into the distilled alcohol intended for human consumption.
The present invention consists in introducing into the fermentation medium (initial sugar-containing must) a concentration close to 0.5 ppm. of a polyether ionophore antibiotic, which has here a bacteriostatic action, that is to say it prevents the bacteria from growing but has no effect on the yeast up to a concentration of 100 ppm. This bacterial flora maintained at 10 4 /ml does not produce any organic acidity and is hence unable to slow down the alcoholic fermentation.
Under these conditions, it cannot produce acrolein either. At doses above 0.5 ppm. of antibiotic, the latter has a bactericidal action and hence makes possible a decline in bacterial content.
The addition of a polyether ionophore antibiotic to the fermentation medium hence makes possible an improved consistency of operation of industrial alcoholic fermentation plants, as well as an improved chemical
S''
A
quality of the distilled alcohol.
1 In a preferred embodiment, the present invention comprises introducing into a fermentation medium at least one polyether ionophore antibiotic in a concentration of from about 0.5 to about 3 ppm. In a particularly preferred embodiment, the concentration of the polyether ionophore antibiotic added is from about 0.5 to about ppm.
The polyether ionophore antibiotics of the present invention do not substantially affect the yeast and that have bacteristatic and/or bactericidal effects on fermentation broth bacteria that produce organic acids. Among the polyether ionophore antibiotics contemplated as useful in the present invention are those that are effective against the bacteria recited in Table 1.
TABLE 1 1 f i ii i
B
Q
:1 j i i r i i i ii i i: r:1
L-'
i TYPICAL BACTERIA ENCOUNTERED IN ALCOHOL FERMENTATION PLANTS Lactobacillus buchneri Lactobacillus plantarum Lactobacillus casei alactosus Lactobacillus casei casei Lactobacillus brevis 2 Lactobacillus brevis 3 Lactobacillus acidophilus Lactobacillus fermentum Lactobacillus lindnerii Leuconostoc mesenteroides Streptococcus equinus Pediococcus pentosaceus Bacillus pumilus Bacillus cereus Clostridium butyricum ijq FrI~l$ORDUACKIBNODELHTEI29477.92 3 The preferred polyether ionophore antibiotics are monensin, lasalosid, salinomycin, narasin, maduramycin, and semduramycin. Monensin, lasalosid, and salinomycin are particularly preferred, while monensin is the most preferred.
Polyether ionophore antibiotics are very stable compounds. They do not easily degrade over time or under high temperatures. This is valuable for fermentation plants since: 1. they remain active for many days under typical fermentation plant operating conditions; ai: 2. they remain active at the high temperatures encountered during the enzymatic hydrolysis of starch that precedes the fermentation of grain or tubers (for example, 2 hours at 90°C or 1.5 hours at 100 0
C).
These compounds are commercially available from pharmaceutical supply firms.
I: 15 i We conducted experiments with several polyether ionophore antibiotics, such as i monensin, lasaalosid, and salinomycin, using fermentation feedstocks derived from sugar beet molasses. These experiments confirmed the existence of bacteriostatic and Sbactericidal concentrations that range from approximately 0.5 ppm to about 1.5 ppm.
S At bacteriostatic concentrations, the growth of the bacterial population stops and no increase in the production of organic acids can be measured. At bactericidal concentrations the bacterial population drops and, consequently, no increase in the production of organic acids can be measured.
The following examples are intended for illustrative purposes and are not intended and should not be construed as limiting the invention in any way.
Example 1 Impact of Monensin on the Concentration of Lactobacillus buchneri.
Varying concentrations of monensin were added to sugar beet molasses derived fermentation feedstocks, and the acidity and bacterial concentration were measured.
The results are presented in Table 2.
4 TABLE 2 INITIAL CONDITIONS Inoculum: 2 x 106 cells/ml Acidity: 1 g/l pH 5.6 Monensin concentration (ppm) 0 0.5 1.0 1.5 2.0 2.5 Bacterial count aft.r 24 hours 4x10 6 8x10 5 104 <10 3 2x10 2 2x10 2 2x10 2 Bacterial count after 48 hours 109 2x10 6 2x10 3 <03 <103 <103 <103 Acidity 5 1 1 1 1 1 1 pH 4.5 5.6 5.6 5.6 5.6 5.6 5.6 Change in acidity 4.2 0 0 0 0 0 0 j it ij y Example 2 Stability and Bactericidal Effect of Monensin in Molasses Juice 1 ppm of monensin was introduced into diluted molasses juice containing 106 bacteria/ml. The bacterial population declined over 20 days at 330C. There was no resumption of growth at any time. This shows that monensin remains active for at least days at 33°C under normal fermentation plant operating conditions.
Example 3 S' Various concentrations of the antibiotic nisin were added to sugar beet molasses derived from fermentation feedstocks, infected with 1.8 x 106 cells/ml of two strains of Lactobacillus, and cultured for 40 hours at 33°C. The bacterial count was measured after dilution in MRS medium. The results are displayed in Table 3.
11 C: WORDUACKIENODELETEM9477.92 r TABLE 3 Lactobacillus Strain No. 1 Lactobacillus Strain No. 2 Nisin (ppm) Bacterial Count (Cells/ml) Nisin (ppm) Bacterial Count (cells/ml) 0 >10 0 1.66 x 2 >10 20 5.6 x 4 8x10 4 40 1 6 1.72 x10 4 60 3 x 8 1.2 x 10 3 80 2 x 1 x10 2 100 7. The results of the test with nisin demonstrate that a decrease in bacterial ce!! ,count was not observed after 48 hours until a minimum of 4 ppm of nisin had been added to the fermentation medium. By contrast, the results of the test with the polyether ionophore monensin (Example 1) show that under similar conditions, as little as 0.5 ppm of monensin resulted in bacteriostasis after 24 hours and 1 ppm of monensin resulted a substantial decrease in bacterial cell count. Thus, monensin is about 4 to 8 times more effective than nisin. This result is completely unexpected and shows the surprising effectiveness of the polyether ionophore antibiotics of the present invention over other known antibiotics.
L I I G4 r 'Z j~ C \WINNIORDUACg!E\NODELErR 9477.92 ills c',
Claims (6)
1. A process for inhibiting bacterial growth in a fermentation medium, including adding any one of the polyether ionophore antibiotics at a concentration close to ppm. to industrial alcoholic fermentation media in order to prevent the growth of bacteria without having an effect on the yeast.
2. A process for reducing the bacterial content of a fermentation medium, including adding any one of the polyether ionophore antibiotics at a concentration above ppm. to industrial fermentation media in order to have a bactericidal action without having an effect on the yeast.
3. Process according to claim 1 or claim 2 wherein the fermentation media treated with the polyether ionophore antibiotics is selected from the following: Sugar-beet juice or molasses Sugar-cane juice or molasses Starch hydrolysates of any origin: cereals or tubers. -Fermented media of wine- or cider-making origin. DATED 4 August, 1995 PHILLIPS ORMONDE FITZPATRICK Attorneys for: UNION NATIONALE DES GROUPEMENT DE DISTILLATEURS D'ALCOOL (U.N.G.D.A) ',Irr i^,JCAWINWORDUACKIENODELETE29477.92 i t ~I i: ji i i i:I i-lr i:; INTERNATIONAL SEARCH REPORT International application No. PCT/FR 92/00984 A. CLASSIFICATION OF SUBJECT MATTER C12N1/16; C12P7/06 According to International Patent Classification (IPC) or to both national classification and IPC B. FIELDS SEARCHED Minimum documentation searched (classification system followed by classification symbols) Int.C1.5 C12N; C12P Documentation searched other than minimum documentation to the extent that such documents are included in the fields searched Electronic data base consulted during the international search (name of data base and. where practicable. search terms used) C. DOCUMENTS CONSIDERED TO BE RELEVANT Category* Citation of document, with indication, where appropriate, of the relevant passages Relevant to claim No. Y INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY 1-2 Vol. 3, No. 3, 1986, NL pages 135 141 S. HENNING ET AL. "New aspects for the application of nisin to food products based on its mode of action" *whole document* Y JOURNAL OF ANIMAL SCIENCE 1-2 Vol. 65, No.
4, 1987, US pages 1064 1076 T.G. NAGARAJA ET AL. "In-vitro lactic acid inhibition and alterations in volatile fatty acid production by antimicrobial feed additives" *whole document* Further documents are listed in the continuation of Box C. See patent family annex. Special categories of cited documents: later document published after the international filing date or pnrity date and not in conflict with the application but cited to understand document defining the general state of the an which is not considered the principle or theor underlying the invention to be of particular relevance earlier document but published on or after the international filing date document of particular relevance: the claimed invention cannot be "L considered novel or cannot be considered to involve an inventive document which may throw doubts on priority claim(s) or which is step when the document is taken alone cited to establish the publication date of another citation or other special reason (as specified) document of particular relevance: the claimed invention cannot be document referring to an oral dislosur use, exhibition or other considered to involve an inventive step when the document is Sdocumen referring o an oral disclosure use, exhibiion or oher combinedwithoneormoreothersuchdocuments.such combination means being obvious to a person skilled in the art document published prior to the international filing date but later than the priority date claimed document member of the same patent family Date of the actual completion of the international search Date of mailing of the international search report February 1993 (05.02.93) 11 March 1993 (11.03.1993) Name and mailing address of the ISA/ Authorized officer EUROPEAN PATENT OFFICE Facsimile No. Telephone No. Form PCT/1SA/210 (second sheet) (July 1992) INTERNATIONAL SEARCH REPORT International application No. PCT/FR 92/00984 C (Continuation). DOCUMENTS CONSIDERED TO BE RELEVANT Category* Citation of document, with indication, where appropriate, of the relevant passages Relevant to claim No. A JOURNAL OF APPLIED BACTERIOLOGY 1-2 Vol. 60, No. 6, June 1986, UK pages 491 500 J.D. PHILLIPS ET AL. "Estimation of Gram-negative bacteria in milk. A comparison of inhibitor systems for preventing Gram-positive bacterial growth" *whole document* Y FR,A,2 587 035 (UNION NATIONALE DES GROUPEMENTS 1-2 DE DISTILLATEURS D'ALCOOL (UNGDA)) 13 March 1987 I *whole document, particularly page 8 lines 1-12* Y CHEMICAL ABSTRACTS, Vol. 97, No. 23, 1-2 6 December 1982, Columbus, Ohio, US; abstract No. 1 9 7321n, F. KUTAS ET AL. "Effect of monensin on rumen acidosis caused by lactic acid fermentation" page 496; column 2; MAGY. ALLATORV. LAPJA 1982, Vol. 37, No. 8, p 535-543 see abstract A FR,A,2 032 598 (DISTILLERIE BRABANT CIE.) 1-2 27 November 1970 *whole document* I on Form PCr/ISA/j10 (continuation of second sheet) (July 1992) F--1 ANNEX TO THE INTERNATIONAL SEARCH REPORT ON INTERNATIONAL PATENT APPLICATION NO. FR 9200984 SA 66753 TIsa annex fists the patent family members reatg to the patent documents cited in the above-emnioned international mearch repor. The members are as contained in die European Patent Office EDP file on The European Patent Office is in no way Lable for them particulars which ame merel given for die purpose of infornation. 05/02/93 Patent documnent Publication patentfamily Publication cited in search repot date member(x) date FR-A-2587035 13-03-87 None FR-A-2032598 27-11-70 None 'W For more dletail about this annex: see officiul Journal of the Eurpea Patent Office, No. 12/82 RAPPORT DE RECHERCHE INTERNATIONALE Demands Intastionale No PCT/FR 92/00984 1. LAS ENETDE INVNI ON(i 2 uiur yblsdoJs~ct Smblo daaletIGSainda t CIB
5 C1NI 1 2N C12P0 Doauaonaizoo ou entation ffloinmn~le olorna.dII am a o des dcm aents foot partie des domaines sur Iciquels Ia recherche a pMOt III. DGMCUNTS CONSIDERES COMMVE PERTINENT.S'o Czligoria fIdentiicaion des documents cztis, avoc inicion, si nbcesszair.P o ie renci Y INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY1- vol. 3, no. 3, 1986, NL pages 135 141 S. HENNING ET AL. 'New aspects for the application of nisin to food products based on its mode of action' *Document en ntier Y JOURNAL OF ANIMAL SCIENCE 1-2 vol. 65, no. 4, 1987, US pages 1064 1076 T.G. NAGARAJA ET AL. 'In-vitro lactic acid inhibition nd alterations in volatile fatty acid production by antimicrobial feed additives' *Document en entier Cat*.garies spbd:Lis e.o documen it dl 1 -T document ultieur publi& posiiouremmnt i I& dane do dipft intornaloaa n i Is daue do piouit6 at n'"aationlas pa document di&bluat rims gdn&a doeI& tochnique, non i rimst de is technique pertinen, reals cite oncapm considiri coma paricuilnfaet pertinent le ptncp on is thbori. coosituat Is bezo de rrlme.3m 'V document antkrieur, mais pubLI6 i Ia date do d6 9 65 itetma- -r documnt paenauamt pertiet; rinventi reanI- tionil ou apris conte dame goes as pow t am addbr~e Comm@ flVOill 00 Co 'V document pouvant jeter on douto sur une revenication do imp~uqot mne vctt~ih invooth priorit OU cite pour ditorminer Is date do izbilcatiOok d'un -r docueat peztimflldreat Petinet; Itnti ros- autet.atin on pour mne raison spidsle (oqidquie) diquke so Pont lage considkrie comma impliqnt mse document so r~firant A me* divulpion caee, i on usage, A ativenti vebi Iomque Is document 49 awscii i on 0m une exposition ou tous antres: mayeas plusioun ,a n, documents de mime Datura, ewte coreid- n- dcuiment pubili avant Ia date do d"p~ international, maijj 051500 ibMn 6vidonto pour one permonn do mier. postktieurement i Is date do priort& revoodqudo W document: quA fit pard. do Is mime af=We do boeW~ 1IV. CERTIFICATION Date i laquei~e Is recherche internationale a *tk effectilwment schevie Date d'expqMition dn rdsait rapport do rcherche intwainaki FEVRIER 1993 IT 0130~ AMministration cbsrgie do la rech~erchec internionale Signature doi foutinaire anttnrisk OFFICE EUROPEEN DES BREVET JULIA P. Fwarmaik PCT/ISA/2lO (dx~ ho.LUN) IJintw 1935) P- I 1, a H PCT/FR '92V00984 Demands In cradonals No (SUITE DES WiSEIGN7EITM INDIQUES SUR 1A 1 IDOCUMNTS CONSIDERES COMME pRTINENTSH DEUXIE7#E FELUE) o Identificition des doamsets cftes, L6 zvec indicationl, siD~far No. des revidadas kgoie des paSSags pelmmtS 1 V Ii~aL A JOURNAL OF APPLIED BACTERIOLOGY 1-2 vol. 60, no.
6, Juin 1986, UK pages 491 500 J.D. PHILLIPS ET AL. 'Estimation of Gram-negative bacteria in milk. A comparison of inhibitor systems for preventing Gram-positive bacterial growth' *Document en entier* Y FR,A,2 587 035 (UNION NATIONALE DES 1-2 GROUPEIIENTS DE DISTILLATEURS D'ALCOOL (UNGDA)) 13 Mars 1987 Document en entier, particulihrement page 8 lignes 1 12 Y CHEMICAL ABSTRACTS, vol. 97, no. 23, 1-2 6 Ddcembre 1982, Columbus, Ohio, US; abstract no. 197321n, F. KUTAS ET AL. 'Effect of monensin on rumen acidosis caused by lactic acid fermentation' page 496 ;colonne 2 MAGY. ALLATORV. LAPJA 1982, vol. 37, no. 8, p 535-543 voir abrdgd A FR,A,2 032 598 (DISTILLERIE BRABANT 1-2 CIE.) 27 Novembre 1970 *Document en entier* Fouaiak PCTIISA11 (hzdW 9aMUesweik (Otabre 1981) ANNEXE AU RAPPORT DE RECHERCHE INTERNATIONALE RELATIF A LA DEMANDE INTERNATIONALE NO. FR 9200984 SA 66753 L2 prisente annext indique los membres de la famili de brevets reatift aux docunmuts brevets citis dams Ie rapport de recherche internationalc visi ci-deus Lesdits niembres sont contemas au fichier infontnatique de I'Office europien des brevets a Ia dat doi Les renseignements founs sont doni i ti"r indicatif et n'engagent pas [a responsabiliti de I'Office europeen des bmwvMs 05/02/93 Documnt brevt diti Date de Mcnmzre(s) de la Date de au rapport de rechemh Publication fainie de brevet(s) pubication FR-A-2587035 13-03-87 Aucun FR-A-2032598 27-11-70 Aucun Pour tout renseignement conceman cette anooe :voir journal officel do roffce mtopeof dies brevetm, No.12/82
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9114176 | 1991-11-18 | ||
| FR9114176A FR2683825B1 (en) | 1991-11-18 | 1991-11-18 | USE OF POLYETHER IONOPHORE ANTIBIOTICS TO LIMIT BACTERIAL GROWTH IN INDUSTRIAL ALCOHOLIC FERMENTATION. |
| PCT/FR1992/000984 WO1993010213A1 (en) | 1991-11-18 | 1992-10-20 | Use of ionophoretic polyether antibiotics for controlling bacterial growth in industrial alcoholic fermentation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2947792A AU2947792A (en) | 1993-06-15 |
| AU663345B2 true AU663345B2 (en) | 1995-10-05 |
Family
ID=9419045
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU29477/92A Expired AU663345B2 (en) | 1991-11-18 | 1992-10-20 | Use of ionophoretic polyether antibiotics for controlling bacterial growth in industrial alcoholic fermentation |
Country Status (19)
| Country | Link |
|---|---|
| EP (1) | EP0615544B1 (en) |
| JP (1) | JP3266908B2 (en) |
| AT (1) | ATE213770T1 (en) |
| AU (1) | AU663345B2 (en) |
| BG (1) | BG61844B1 (en) |
| BR (1) | BR9206769A (en) |
| CA (1) | CA2123681C (en) |
| CZ (1) | CZ122194A3 (en) |
| DE (1) | DE69232441T2 (en) |
| EC (1) | ECSP930934A (en) |
| ES (1) | ES2170057T3 (en) |
| FI (1) | FI942283A0 (en) |
| FR (1) | FR2683825B1 (en) |
| HU (1) | HU215554B (en) |
| OA (1) | OA09925A (en) |
| RU (1) | RU2104301C1 (en) |
| SK (1) | SK56294A3 (en) |
| UA (1) | UA27128C2 (en) |
| WO (1) | WO1993010213A1 (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2697723B1 (en) * | 1992-11-06 | 1995-03-03 | Ungda | Use of polyether ionophoric antibiotics in industrial extraction or production of sweet products. |
| EA030926B1 (en) * | 2009-02-11 | 2018-10-31 | Ксилеко, Инк. | Saccharifying biomass |
| BRPI0900238A2 (en) * | 2009-02-12 | 2010-10-26 | Arch Chem Inc | antimicrobial composition and process for controlling microbial contamination in alcoholic fermentation processes |
| BR122019003653B1 (en) * | 2010-03-19 | 2020-11-10 | Buckman Laboratories International, Inc | method to produce ethanol |
| JP6004321B2 (en) * | 2012-04-18 | 2016-10-05 | 日立造船株式会社 | Methods for controlling the growth of miscellaneous bacteria in ethanol fermentation of moss |
| CN107074900A (en) | 2014-09-19 | 2017-08-18 | 希乐克公司 | Saccharide and saccharide compositions and mixtures |
| RU2584603C1 (en) * | 2015-04-24 | 2016-05-20 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Московский государственный университет пищевых производств" Министерства образования и науки Российской Федерации | Method for antibacterial treatment of yeast |
| EP3559247A2 (en) * | 2016-12-22 | 2019-10-30 | Synata Bio, Inc. | Methods and systems using ionophores to control contamination in fermentation of gaseous substrates |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2032598A5 (en) * | 1970-01-07 | 1970-11-27 | Brabant Et Cie Distiller | Continuous fermentation of molasses and - other alcohol formers |
| FR2587035B1 (en) * | 1985-09-09 | 1989-08-04 | Ungda | PROCESS FOR THE PRODUCTION OF ETHANOL BY FERMENTATION OF SUGAR MEDIA |
-
1991
- 1991-11-18 FR FR9114176A patent/FR2683825B1/en not_active Expired - Lifetime
-
1992
- 1992-10-20 AU AU29477/92A patent/AU663345B2/en not_active Expired
- 1992-10-20 SK SK562-94A patent/SK56294A3/en unknown
- 1992-10-20 EP EP92923846A patent/EP0615544B1/en not_active Expired - Lifetime
- 1992-10-20 AT AT92923846T patent/ATE213770T1/en not_active IP Right Cessation
- 1992-10-20 CA CA002123681A patent/CA2123681C/en not_active Expired - Lifetime
- 1992-10-20 BR BR9206769A patent/BR9206769A/en not_active IP Right Cessation
- 1992-10-20 JP JP50901993A patent/JP3266908B2/en not_active Expired - Fee Related
- 1992-10-20 UA UA94005379A patent/UA27128C2/en unknown
- 1992-10-20 WO PCT/FR1992/000984 patent/WO1993010213A1/en active IP Right Grant
- 1992-10-20 ES ES92923846T patent/ES2170057T3/en not_active Expired - Lifetime
- 1992-10-20 DE DE69232441T patent/DE69232441T2/en not_active Expired - Lifetime
- 1992-10-20 RU RU94027279/13A patent/RU2104301C1/en not_active IP Right Cessation
- 1992-10-20 HU HU9401536A patent/HU215554B/en not_active IP Right Cessation
- 1992-10-20 CZ CZ941221A patent/CZ122194A3/en unknown
-
1993
- 1993-05-28 EC EC1993000934A patent/ECSP930934A/en unknown
-
1994
- 1994-05-17 FI FI942283A patent/FI942283A0/en unknown
- 1994-05-17 BG BG98779A patent/BG61844B1/en unknown
- 1994-05-18 OA OA60513A patent/OA09925A/en unknown
Non-Patent Citations (1)
| Title |
|---|
| HENNING ET AL: FOOS MICRO, 3, PP135-141 (1986) * |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH08500002A (en) | 1996-01-09 |
| BG98779A (en) | 1995-05-31 |
| AU2947792A (en) | 1993-06-15 |
| OA09925A (en) | 1994-09-15 |
| HU9401536D0 (en) | 1994-08-29 |
| WO1993010213A1 (en) | 1993-05-27 |
| BR9206769A (en) | 1995-10-31 |
| ATE213770T1 (en) | 2002-03-15 |
| HU215554B (en) | 1999-01-28 |
| JP3266908B2 (en) | 2002-03-18 |
| EP0615544B1 (en) | 2002-02-27 |
| SK56294A3 (en) | 1995-11-08 |
| UA27128C2 (en) | 2000-02-28 |
| CA2123681A1 (en) | 1993-05-27 |
| CA2123681C (en) | 2001-08-14 |
| FR2683825A1 (en) | 1993-05-21 |
| FI942283A (en) | 1994-05-17 |
| RU2104301C1 (en) | 1998-02-10 |
| CZ122194A3 (en) | 1994-12-15 |
| ECSP930934A (en) | 1994-03-21 |
| EP0615544A1 (en) | 1994-09-21 |
| FI942283A0 (en) | 1994-05-17 |
| DE69232441T2 (en) | 2002-10-31 |
| ES2170057T3 (en) | 2002-08-01 |
| BG61844B1 (en) | 1998-07-31 |
| FR2683825B1 (en) | 1995-01-06 |
| DE69232441D1 (en) | 2002-04-04 |
| HUT67063A (en) | 1995-01-30 |
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