AU650885B2 - Method for detecting a nucleotide sequence by sandwich hybridization - Google Patents

Method for detecting a nucleotide sequence by sandwich hybridization

Info

Publication number
AU650885B2
AU650885B2 AU79953/91A AU7995391A AU650885B2 AU 650885 B2 AU650885 B2 AU 650885B2 AU 79953/91 A AU79953/91 A AU 79953/91A AU 7995391 A AU7995391 A AU 7995391A AU 650885 B2 AU650885 B2 AU 650885B2
Authority
AU
Australia
Prior art keywords
nucleotide sequence
detecting
fixed
sandwich hybridization
hybridization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU79953/91A
Other versions
AU7995391A (en
Inventor
Patrice Allibert
Philippe Cros
Claude Mabilat
Francois Mallet
Bernard Mandrand
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
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Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=9397483&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU650885(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Biomerieux SA filed Critical Biomerieux SA
Publication of AU7995391A publication Critical patent/AU7995391A/en
Application granted granted Critical
Publication of AU650885B2 publication Critical patent/AU650885B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Oncology (AREA)
  • AIDS & HIV (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Inert Electrodes (AREA)
  • Current-Collector Devices For Electrically Propelled Vehicles (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method for detecting a single-stranded nucleotide sequence in a sample which is known or thought to contain it, using sandwich hybridization comprising the step of incubating the sample with a capture probe which is passively fixed to a solid support and with a detector probe which is labelled with a non-radioactive marker. The capture and detector probes can hybridize with two non-overlapping regions respectively of the target nucleotide sequence being sought. The incubation step is followed by a washing step for removing reagents which have not been fixed by hybridization. The method is characterized in that the capture probe contains 9-30 nucleotides and is fixed to said solid support which is made of a material based on a hydrophobic compound. The method can be used particularly for diagnosing infectious or genetic diseases and for cell typing.
AU79953/91A 1990-06-11 1991-06-11 Method for detecting a nucleotide sequence by sandwich hybridization Ceased AU650885B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9007249A FR2663040B1 (en) 1990-06-11 1990-06-11 METHOD OF DETECTING A NUCLEOTIDE SEQUENCE ACCORDING TO THE SANDWICH HYBRIDIZATION TECHNIQUE.
FR9007249 1990-06-11
PCT/FR1991/000468 WO1991019812A1 (en) 1990-06-11 1991-06-11 Method for detecting a nucleotide sequence by sandwich hybridization

Publications (2)

Publication Number Publication Date
AU7995391A AU7995391A (en) 1992-01-07
AU650885B2 true AU650885B2 (en) 1994-07-07

Family

ID=9397483

Family Applications (1)

Application Number Title Priority Date Filing Date
AU79953/91A Ceased AU650885B2 (en) 1990-06-11 1991-06-11 Method for detecting a nucleotide sequence by sandwich hybridization

Country Status (12)

Country Link
EP (1) EP0486661B1 (en)
JP (1) JP3289089B2 (en)
AT (1) ATE133995T1 (en)
AU (1) AU650885B2 (en)
CA (1) CA2059657C (en)
DE (1) DE69116993T2 (en)
DK (1) DK0486661T3 (en)
ES (1) ES2084167T3 (en)
FI (1) FI102296B (en)
FR (1) FR2663040B1 (en)
PT (1) PT97939B (en)
WO (1) WO1991019812A1 (en)

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CA1276575C (en) * 1984-11-30 1990-11-20 Sylvie Beaudenon Papillomavirus probes and process for the in vitro diagnostic of papillomavirus infections
JP2633275B2 (en) * 1986-03-21 1997-07-23 アンステイテユ・パストウール DNA or DNA fragment derived from the genome of papilloma virus (HPV) -33
CA2052413C (en) * 1990-09-28 2004-07-13 Jeffrey L. Joseph Nucleotides sequences useful as type-specific probes, pcr primers and lcr probes for the amplifications and detection of human papilloma virus, and related kits and methods
FR2679255B1 (en) * 1991-07-17 1993-10-22 Bio Merieux METHOD OF IMMOBILIZING A NUCLEIC FRAGMENT BY PASSIVE FIXING ON A SOLID SUPPORT, SOLID SUPPORT THUS OBTAINED AND ITS USE.
DE69233630T2 (en) * 1991-12-23 2007-05-31 Chiron Corp., Emeryville Sets of HIV probes for use in solution phase sandwich hydride assays
US5656432A (en) * 1992-02-10 1997-08-12 Bio Merieux Genomic DNA fragment of Streptococcus pneumoniae, hybridization probe, amplification primer, reagent and method for the detection of Streptococcus pneumoniae
FR2687168B1 (en) * 1992-02-10 1994-03-25 Bio Merieux FRAGMENT OF STREPTOCOCCUS PNEUMONIAE GENOMIC DNA, HYBRIDIZATION PROBE, AMPLIFIER PRIME, REAGENT AND METHOD FOR DETECTION OF STREPTOCOCCUS PNEUMONIAE.
WO1994002636A1 (en) * 1992-07-28 1994-02-03 Hitachi Chemical Company Ltd. Gene detection system
FR2694754B1 (en) * 1992-08-12 1994-09-16 Bio Merieux Mycobacteria DNA fragments, amplification primers, hybridization probes, reagents and method for detecting detection of mycobacteria.
FR2701961B1 (en) * 1993-02-24 1995-04-21 Bio Merieux Method for destabilizing an intracatenary secondary structure of a single-stranded polynucleotide, and for capturing said nucleotide.
AU6945694A (en) * 1993-05-06 1994-12-12 Dade International Inc. Human papillomavirus detection assay
FR2706618B1 (en) * 1993-06-11 1995-09-01 Bio Merieux Device for the dosage of haptens and its use.
FR2707010B1 (en) * 1993-06-25 1995-09-29 Bio Merieux
US5601978A (en) * 1993-09-03 1997-02-11 Abbott Laboratories Oligonucleotides and methods for the detection of chlamydia trachomatis
FR2710075B1 (en) * 1993-09-15 1995-10-27 Bio Merieux Reagent and method for the detection of a nucleotide sequence with signal amplification.
US5610287A (en) * 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
FR2726277B1 (en) 1994-10-28 1996-12-27 Bio Merieux OLIGONUCLEOTIDE FOR USE AS PRIMER IN AN AMPLIFICATION METHOD BASED ON REPLICATION WITH MOVEMENT OF STRAND
FR2728687A1 (en) * 1994-12-21 1996-06-28 Bio Merieux METHOD FOR DETERMINING A HAPTENE ACCORDING TO A COMPETITION TECHNIQUE
FR2730545B1 (en) * 1995-02-13 1997-03-28 Bio Merieux FROZEN STATIC VALVE AND PROCESSING ENCLOSURE CONTROLLED BY AT LEAST ONE VALVE
FR2733755B1 (en) * 1995-05-03 1997-07-11 Bio Merieux NUCLEOTIDE FRAGMENT OF 16S RIBOSOME RNA FROM CORYNEBACTERIA, PROBES AND DERIVED PRIMERS, REAGENT AND DETECTION METHOD
RU2146707C1 (en) * 1996-04-11 2000-03-20 Куликова Валентина Филипповна Method of detection of analyzed sequence of nucleic acid
BE1011052A3 (en) * 1997-03-20 1999-04-06 Jose Remacle Diagnostic and/or assay method and kit using sandwich type hybridisation of nucleic acid sequences on solid substrate
GB9620075D0 (en) * 1996-09-26 1996-11-13 Dynal As Method
EP0843019A3 (en) * 1996-11-08 2002-10-09 Kyowa Medex Co., Ltd. Method for determination of specific nucleic acid sequence, and a reagent therefor
JP4185185B2 (en) * 1997-05-08 2008-11-26 征夫 軽部 DNA detection method using partial double-stranded DNA
GB9712483D0 (en) 1997-06-17 1997-08-20 Kathirgamanathan Poopathy Fabrication of light emitting devices from chelates of transition metals, lanthanides and actinides
US6001558A (en) * 1997-06-25 1999-12-14 Ortho Clinical Diagnostics, Inc. Amplification and detection of HIV-1 and/or HIV 2
IT1297096B1 (en) * 1997-12-03 1999-08-03 Sorin Diagnostics S R L METHOD FOR THE DETECTION OF SINGLE HELIX DNA ANALYTES
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FR2797889A1 (en) 1999-09-01 2001-03-02 Bio Merieux METHOD FOR DETECTING THE EXPRESSION OF ENVELOPE PROTEIN FROM A HUMAN ENDOGENEOUS RETROVIRUS AND USES OF A GENE ENCODING SAME
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Also Published As

Publication number Publication date
WO1991019812A1 (en) 1991-12-26
JP3289089B2 (en) 2002-06-04
FR2663040A1 (en) 1991-12-13
EP0486661B1 (en) 1996-02-07
PT97939B (en) 2001-05-31
FI920552A0 (en) 1992-02-10
FR2663040B1 (en) 1995-09-15
CA2059657C (en) 2003-01-07
PT97939A (en) 1992-03-31
EP0486661A1 (en) 1992-05-27
FI102296B1 (en) 1998-11-13
AU7995391A (en) 1992-01-07
FI102296B (en) 1998-11-13
DE69116993T2 (en) 1996-11-14
ES2084167T3 (en) 1996-05-01
ATE133995T1 (en) 1996-02-15
JPH05501957A (en) 1993-04-15
DE69116993D1 (en) 1996-03-21
CA2059657A1 (en) 1991-12-12
DK0486661T3 (en) 1996-06-17

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