AU4646885A - Synthetic regulation region - Google Patents

Synthetic regulation region

Info

Publication number
AU4646885A
AU4646885A AU46468/85A AU4646885A AU4646885A AU 4646885 A AU4646885 A AU 4646885A AU 46468/85 A AU46468/85 A AU 46468/85A AU 4646885 A AU4646885 A AU 4646885A AU 4646885 A AU4646885 A AU 4646885A
Authority
AU
Australia
Prior art keywords
promotor
region
ribosome
pairs
nucleotide sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU46468/85A
Other versions
AU592062B2 (en
Inventor
Joachim Engels
Michael Leineweber
Eugen Uhlmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of AU4646885A publication Critical patent/AU4646885A/en
Application granted granted Critical
Publication of AU592062B2 publication Critical patent/AU592062B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/57—IFN-gamma

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1. Claims (for the Contracting States : BE, CH, DE, FR, GB, IT, LI, LU, NL and SE) A synthetic regulation region for the expression of heterologous genes in E. coli, containing a promotor, a modified lac operator and a ribosome-binding site, characterized in that a) the -35 region in the promotor has the nucleotide sequence (coding strand) TTGACAT or CTTGACAT b) the -10 region in the promotor has the nucleotide sequence (coding strand) GTATAAT, c) a spacer group of 14 to 17 base-pairs is between the -35 and the -10 regions, and d) a spacer group of 6 to 14 base-pairs is located between the ribosome-binding site and the ATG start codon. 1. Claims (for the Contracting State AT) A process for the expression of heterologous genes in E. coli, characterized by placing, upstream of the heterologous gene in the direction of reading, a synthetic regulation region which contains a promotor, a modified lac operator and a ribosome-binding site, in which a) the -35 region in the promotor has the nucleotide sequence (coding strand) TTGACAT or CTTGACAT b) the -10 region in the promotor has the nucleotide sequence (coding strand) GTATAAT, c) a spacer group of 14 to 17 base-pairs is between the -35 and the -10 regions, and d) a spacer group of 6 to 14 base-pairs is located between the ribosome-binding site and the ATG start codon.
AU46468/85A 1984-08-21 1985-08-20 Synthetic regulation region Ceased AU592062B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3430683 1984-08-21
DE19843430683 DE3430683A1 (en) 1984-08-21 1984-08-21 SYNTHETIC REGULATION REGION

Publications (2)

Publication Number Publication Date
AU4646885A true AU4646885A (en) 1986-02-27
AU592062B2 AU592062B2 (en) 1990-01-04

Family

ID=6243523

Family Applications (1)

Application Number Title Priority Date Filing Date
AU46468/85A Ceased AU592062B2 (en) 1984-08-21 1985-08-20 Synthetic regulation region

Country Status (13)

Country Link
EP (1) EP0173149B1 (en)
JP (1) JPS6158591A (en)
KR (1) KR870002259A (en)
AT (1) ATE46538T1 (en)
AU (1) AU592062B2 (en)
DE (2) DE3430683A1 (en)
DK (1) DK378385A (en)
ES (1) ES8605033A1 (en)
FI (1) FI853177L (en)
GR (1) GR852000B (en)
HU (1) HU197353B (en)
PT (1) PT80989B (en)
ZA (1) ZA856304B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU600229B2 (en) * 1985-04-19 1990-08-09 Hoechst Aktiengesellschaft Modification of the DNA sequemce between the shine-dalgarno sequence and the start codon of the TRP operon to increase protein expression
US5268284A (en) * 1988-03-11 1993-12-07 The Upjohn Company Ribosome binding site

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2598430B1 (en) * 1986-05-06 1990-02-02 Roussel Uclaf NOVEL EXPRESSION VECTORS, PROCESS FOR OBTAINING SAME, AND APPLICATION THEREOF FOR EXPRESSING ANY PROTEIN IN ESCHERICHIA COLI
EP0372005A4 (en) * 1987-10-30 1990-06-26 Oncogen Expression systems for preparation of polypeptides in prokaryotic cells.
JPH03503360A (en) * 1988-03-11 1991-08-01 ジ・アップジョン・カンパニー ribosome binding site
GR1002113B (en) * 1988-09-02 1996-02-02 Oncogen Expression systems for preparation of polypeptides in prokaryotic cells
US6689604B1 (en) * 1998-03-20 2004-02-10 National Research Council Of Canada Lipopolysaccharide α-2,3 sialyltransferase of Campylobacter jejuni and its uses

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL65775A (en) * 1981-05-18 1985-05-31 Genentech Inc Microbial hybrid promotors and plasmids and e.coli comprising them
AU575301B2 (en) * 1982-10-08 1988-07-28 Juridical Foundation, Japanese Foundation For Cancer Research Novel vector
GR81701B (en) * 1983-01-18 1984-12-12 Lilly Co Eli
FI843351A (en) * 1983-08-26 1985-02-27 Du Pont EXPRESSIONSPLASMIDER FOER PRODUKTION AV SPECIFIKA PROTEINER.

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU600229B2 (en) * 1985-04-19 1990-08-09 Hoechst Aktiengesellschaft Modification of the DNA sequemce between the shine-dalgarno sequence and the start codon of the TRP operon to increase protein expression
US5268284A (en) * 1988-03-11 1993-12-07 The Upjohn Company Ribosome binding site

Also Published As

Publication number Publication date
DE3573134D1 (en) 1989-10-26
ZA856304B (en) 1986-04-30
ES546242A0 (en) 1986-03-01
KR870002259A (en) 1987-03-30
DE3430683A1 (en) 1986-03-06
DK378385D0 (en) 1985-08-20
AU592062B2 (en) 1990-01-04
PT80989B (en) 1987-11-11
GR852000B (en) 1985-12-18
HUT39207A (en) 1986-08-28
ATE46538T1 (en) 1989-10-15
HU197353B (en) 1989-03-28
DK378385A (en) 1986-02-22
FI853177L (en) 1986-02-22
EP0173149A1 (en) 1986-03-05
PT80989A (en) 1985-09-01
ES8605033A1 (en) 1986-03-01
JPS6158591A (en) 1986-03-25
EP0173149B1 (en) 1989-09-20
FI853177A0 (en) 1985-08-19

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