AU4646885A - Synthetic regulation region - Google Patents
Synthetic regulation regionInfo
- Publication number
- AU4646885A AU4646885A AU46468/85A AU4646885A AU4646885A AU 4646885 A AU4646885 A AU 4646885A AU 46468/85 A AU46468/85 A AU 46468/85A AU 4646885 A AU4646885 A AU 4646885A AU 4646885 A AU4646885 A AU 4646885A
- Authority
- AU
- Australia
- Prior art keywords
- promotor
- region
- ribosome
- pairs
- nucleotide sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/57—IFN-gamma
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
1. Claims (for the Contracting States : BE, CH, DE, FR, GB, IT, LI, LU, NL and SE) A synthetic regulation region for the expression of heterologous genes in E. coli, containing a promotor, a modified lac operator and a ribosome-binding site, characterized in that a) the -35 region in the promotor has the nucleotide sequence (coding strand) TTGACAT or CTTGACAT b) the -10 region in the promotor has the nucleotide sequence (coding strand) GTATAAT, c) a spacer group of 14 to 17 base-pairs is between the -35 and the -10 regions, and d) a spacer group of 6 to 14 base-pairs is located between the ribosome-binding site and the ATG start codon. 1. Claims (for the Contracting State AT) A process for the expression of heterologous genes in E. coli, characterized by placing, upstream of the heterologous gene in the direction of reading, a synthetic regulation region which contains a promotor, a modified lac operator and a ribosome-binding site, in which a) the -35 region in the promotor has the nucleotide sequence (coding strand) TTGACAT or CTTGACAT b) the -10 region in the promotor has the nucleotide sequence (coding strand) GTATAAT, c) a spacer group of 14 to 17 base-pairs is between the -35 and the -10 regions, and d) a spacer group of 6 to 14 base-pairs is located between the ribosome-binding site and the ATG start codon.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE3430683 | 1984-08-21 | ||
DE19843430683 DE3430683A1 (en) | 1984-08-21 | 1984-08-21 | SYNTHETIC REGULATION REGION |
Publications (2)
Publication Number | Publication Date |
---|---|
AU4646885A true AU4646885A (en) | 1986-02-27 |
AU592062B2 AU592062B2 (en) | 1990-01-04 |
Family
ID=6243523
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU46468/85A Ceased AU592062B2 (en) | 1984-08-21 | 1985-08-20 | Synthetic regulation region |
Country Status (13)
Country | Link |
---|---|
EP (1) | EP0173149B1 (en) |
JP (1) | JPS6158591A (en) |
KR (1) | KR870002259A (en) |
AT (1) | ATE46538T1 (en) |
AU (1) | AU592062B2 (en) |
DE (2) | DE3430683A1 (en) |
DK (1) | DK378385A (en) |
ES (1) | ES8605033A1 (en) |
FI (1) | FI853177L (en) |
GR (1) | GR852000B (en) |
HU (1) | HU197353B (en) |
PT (1) | PT80989B (en) |
ZA (1) | ZA856304B (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU600229B2 (en) * | 1985-04-19 | 1990-08-09 | Hoechst Aktiengesellschaft | Modification of the DNA sequemce between the shine-dalgarno sequence and the start codon of the TRP operon to increase protein expression |
US5268284A (en) * | 1988-03-11 | 1993-12-07 | The Upjohn Company | Ribosome binding site |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2598430B1 (en) * | 1986-05-06 | 1990-02-02 | Roussel Uclaf | NOVEL EXPRESSION VECTORS, PROCESS FOR OBTAINING SAME, AND APPLICATION THEREOF FOR EXPRESSING ANY PROTEIN IN ESCHERICHIA COLI |
EP0372005A4 (en) * | 1987-10-30 | 1990-06-26 | Oncogen | Expression systems for preparation of polypeptides in prokaryotic cells. |
JPH03503360A (en) * | 1988-03-11 | 1991-08-01 | ジ・アップジョン・カンパニー | ribosome binding site |
GR1002113B (en) * | 1988-09-02 | 1996-02-02 | Oncogen | Expression systems for preparation of polypeptides in prokaryotic cells |
US6689604B1 (en) * | 1998-03-20 | 2004-02-10 | National Research Council Of Canada | Lipopolysaccharide α-2,3 sialyltransferase of Campylobacter jejuni and its uses |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL65775A (en) * | 1981-05-18 | 1985-05-31 | Genentech Inc | Microbial hybrid promotors and plasmids and e.coli comprising them |
AU575301B2 (en) * | 1982-10-08 | 1988-07-28 | Juridical Foundation, Japanese Foundation For Cancer Research | Novel vector |
GR81701B (en) * | 1983-01-18 | 1984-12-12 | Lilly Co Eli | |
FI843351A (en) * | 1983-08-26 | 1985-02-27 | Du Pont | EXPRESSIONSPLASMIDER FOER PRODUKTION AV SPECIFIKA PROTEINER. |
-
1984
- 1984-08-21 DE DE19843430683 patent/DE3430683A1/en not_active Withdrawn
-
1985
- 1985-08-09 EP EP85110050A patent/EP0173149B1/en not_active Expired
- 1985-08-09 AT AT85110050T patent/ATE46538T1/en not_active IP Right Cessation
- 1985-08-09 DE DE8585110050T patent/DE3573134D1/en not_active Expired
- 1985-08-13 HU HU853102A patent/HU197353B/en not_active IP Right Cessation
- 1985-08-16 GR GR852000A patent/GR852000B/el unknown
- 1985-08-19 FI FI853177A patent/FI853177L/en not_active Application Discontinuation
- 1985-08-19 ES ES546242A patent/ES8605033A1/en not_active Expired
- 1985-08-20 KR KR1019850005980A patent/KR870002259A/en not_active Application Discontinuation
- 1985-08-20 PT PT80989A patent/PT80989B/en not_active IP Right Cessation
- 1985-08-20 JP JP60181149A patent/JPS6158591A/en active Pending
- 1985-08-20 AU AU46468/85A patent/AU592062B2/en not_active Ceased
- 1985-08-20 DK DK378385A patent/DK378385A/en not_active Application Discontinuation
- 1985-08-20 ZA ZA856304A patent/ZA856304B/en unknown
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU600229B2 (en) * | 1985-04-19 | 1990-08-09 | Hoechst Aktiengesellschaft | Modification of the DNA sequemce between the shine-dalgarno sequence and the start codon of the TRP operon to increase protein expression |
US5268284A (en) * | 1988-03-11 | 1993-12-07 | The Upjohn Company | Ribosome binding site |
Also Published As
Publication number | Publication date |
---|---|
DE3573134D1 (en) | 1989-10-26 |
ZA856304B (en) | 1986-04-30 |
ES546242A0 (en) | 1986-03-01 |
KR870002259A (en) | 1987-03-30 |
DE3430683A1 (en) | 1986-03-06 |
DK378385D0 (en) | 1985-08-20 |
AU592062B2 (en) | 1990-01-04 |
PT80989B (en) | 1987-11-11 |
GR852000B (en) | 1985-12-18 |
HUT39207A (en) | 1986-08-28 |
ATE46538T1 (en) | 1989-10-15 |
HU197353B (en) | 1989-03-28 |
DK378385A (en) | 1986-02-22 |
FI853177L (en) | 1986-02-22 |
EP0173149A1 (en) | 1986-03-05 |
PT80989A (en) | 1985-09-01 |
ES8605033A1 (en) | 1986-03-01 |
JPS6158591A (en) | 1986-03-25 |
EP0173149B1 (en) | 1989-09-20 |
FI853177A0 (en) | 1985-08-19 |
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