AU4579593A - Tetracyclic compounds process and intermediates for their preparation and their use as antitumour agents - Google Patents
Tetracyclic compounds process and intermediates for their preparation and their use as antitumour agentsInfo
- Publication number
- AU4579593A AU4579593A AU45795/93A AU4579593A AU4579593A AU 4579593 A AU4579593 A AU 4579593A AU 45795/93 A AU45795/93 A AU 45795/93A AU 4579593 A AU4579593 A AU 4579593A AU 4579593 A AU4579593 A AU 4579593A
- Authority
- AU
- Australia
- Prior art keywords
- alkyl
- carbazole
- carboxylate
- aryl
- formula
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D207/00—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D207/02—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
- C07D207/30—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members
- C07D207/32—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
- C07D207/33—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms with substituted hydrocarbon radicals, directly attached to ring carbon atoms
- C07D207/333—Radicals substituted by oxygen or sulfur atoms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D207/00—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D207/02—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
- C07D207/30—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members
- C07D207/34—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/56—Ring systems containing three or more rings
- C07D209/80—[b, c]- or [b, d]-condensed
- C07D209/94—[b, c]- or [b, d]-condensed containing carbocyclic rings other than six-membered
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
- C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
- C07D403/06—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
- C07D409/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings
- C07D409/06—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
- C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
- C07D487/04—Ortho-condensed systems
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
- C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
- C07D491/04—Ortho-condensed systems
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D495/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
- C07D495/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
- C07D495/04—Ortho-condensed systems
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
- Plural Heterocyclic Compounds (AREA)
Description
TETRACYCLIC COMPOUNDS PROCESS AND INTERMEDIATES FOR THEIR PREPARATION AND THEIR USE AS ANTITUMOUR AGENTS
The present invention relates to heterocyclic compounds which have been found to have anti-tumour activity. More specifically, the invention concerns Pyrrolo [3,2-b] carbazoles, 1H-Benzofuro [3,2-f] indoles and 1H-[1] Benzothieno [2,3-f] indoles, methods for their preparation, pharmaceutical formulations containing them and their use as anti-tumour agents.
Research in the area of cancer chemotherapy has produced a variety of anti-tumour agents, which have differing degrees of efficacy. Standard clinically used agents include adriamycin, actinomycin D, methotrexate, 5-fluorouracil, cis-platinum, vincristine and vinblastine. However, these presently available anti-tumour agents are known to have various disadvantages, such as toxicity to healthy cells and resistance to certain tumour types.
There thus exists a continuing need to develop new and improved anti-tumour agents.
Khoshtariya et al, khim. Geterotsikl. Soedin (1980), (2) 203-8, disclose the synthesis of certain indolobenzo[b] thiophenes.
Khoshtariya et al, khim Geterotsikl Soedin (1984), (10) 1366-70 disclose the synthesis of certain indolobenzo[b] furans.
Kakhabrishvili et al, khim Geterotsikl Soedin (1985), (3) 355-8 disclose the synthesis of certain derivatives of indolo[5,6-d] and indolo [5,4-d] benzo[b] furans
The patent specification EP447,703 discloses the synthesis of certain benzo(5,6-b)benzofuran-2-carboxylates.
There have now been discovered novel compounds which exhibit anti-tumour cell activity with low toxicity against normal cell lines.
Thus, in a first aspect the present invention provides a compound of the general formula (1)
and salts and physiologically functional derivatives thereof, wherein A is
X is O, S, SO, SO2, CH2, CO or NR7, wherein R7 is H, alkyl, aralkyl, aryl, alkenyl, acyl, alkynyl, sulphonyl or substituted sulphonyl;
Y is O, S, SO, SO2, CH2, CO or NR7;
R1 is COR8, COOR8, CHO, CH2OH, CH2OR9, CONH2, CONHNR10R11 ,
CONHR10, CONR1 0R11, COO(CH2)nNR10R11, wherein R8 is H, alkyl, aryl, substituted aryl or aralkyl, R9 is acyl or substituted acyl, R10 and R11 are independently hydrogen, alkyl or aryl, and n is 1 to 4 carbon atoms; R2 is H, COOR8, alkyl, aryl, substituted aryl or CH2CH2CO2R12 wherein R12 is alkyl or aryl; R3 and R4 are independently H,hydroxy, alkyl, haloalkyl, alkoxy, halo, cyano, nitro, amino, alkyl amino, dialkyl amino, substituted alkyl, carboxyl or CO2R12;
R5 is H, alkyl, substituted alkyl, aralkyl, nitro, amino, halo, cyano, CHO, COOR8; R6 is H, aryl, alkyl, aralkyl, nitro, halogen, CHO or COR13 wherein R13 is alkyl or aryl with the proviso that (i) when R2, R3, R4, R5 and R6 are all H and A is
wherein Y is NH and X is O or S, then R1 is not CO2H or CO2Et; and (ii) when R2, R3, R4, R5 and R6 are all H and A is
wherein Y is NH, and X is O then R1 is not CHO; and
(iii) Y is not O when X is O.
In yet a further aspect the present invention provides a compound of the general formula (1) above, wherein A is
X is O, S, SO, SO2, CH2, CO or NR7, wherein R7 is H, alkyl, aralkyl, aryl, alkenyl, acyl, alkynyl or sulphonyl;
Y is O, S, SO, SO2, CH2, CO or NR7 ;
R1 is COOR8,CHO,CH2OH, CH2OR9, CONH2, CONHR10 or CONR10R11, wherein R8 is H, alkyl, aryl, substituted aryl or aralkyl, R9 is acyl or substituted acyl, and R10 and R11 are independently alkyl or aryl; R2 is H, COOR8, alkyl,aryl, substituted aryl or CH2CH2COR11 wherein R12 is alkyl or aryl; R3 and R4 are independently H, hydroxy, alkyl, haloalkyl, alkoxy, halo, cyano, nitro, amino, alkyl amino, dialkyl amino, substituted alkyl, carboxyl or CO2R12; R5 is H, alkyl, substituted alkyl, aralkyl, nitro, halo, cyano CHO; R6 is H, alkyl, aralkyl, nitro, halo, CHO or COR13
wherein R13 is alkyl or aryl with the proviso described above.
Alkyl groups present in general formula (I) may be straight or branched chain alkyl groups, and may contain 1-10 carbon atoms and suitably 1-6 carbon atoms. Examples of such alkyl groups include methyl, ethyl, t-butyl and the like.
Acyl groups may be straight or branched and may contain 1-10 carbon atoms and suitably 1-6 carbon atoms. Examples of suitable acyl groups include ethanoyl and propanoyl groups.
Alkoxy may be straight or branched and may contain 1-10 carbon atoms and suitably 1-6 carbon atoms. Examples of suitable alkoxy groups include methoxy, ethoxy and the like.
Aryl includes both carbocyclic aryl groups and heterocyclic aryl groups normally containing a maximum of 10 ring atoms. Carbocyclic aryl groups include, eg phenyl and naphthyl and contain at least one aromatic ring. Heterocyclic aryl groups include eg thienyl, furyl, pyridyl, indole and quinoline rings.
An aralkyl group may contain from 1 to 4 atoms in the alkyl portion and the aryl portion may be a carbocyclic or heterocyclic aryl group.
Substituents which may be present on the alkyl, aryl or acyl groups include alkyl, alkoxy, halo, sulphinyl, amino (optionally substituted by one or two alkyl groups), haloalkyl (eg trifluoromethyl), sulphinyl, sulphonyl and cyano.
Substituents which may be present on the sulphonyl include alkyl, aryl and aralkyl.
Halogen represents fluoro, chloro, bromo or iodo.
In the compounds of formula (1)
X is preferably O, S or NR7, wherein R7 is H, alkyl, sulphonyl or toluene sulphonyl; Y is preferably NR7;
R1 is preferably COR8, COOR8, CH2OR9, CONH2, CNHNR10R11, CONHR10,
CONR10R11, COO(CH2)n 10NR10R11, wherein R8 is H, alkyl, aryl, substituted aryl or aralkyl, R is acyl or substituted acyl, and R10 and R11 are independently hydrogen, alkyl or aryl and n is 1 to 4 carbon atoms; R2 is preferably COOR8, alkyl or CH2CH2CO2R12 wherein R12 is alkyl or aryl; R3 and R4 represent independently H,hydroxy, alkyl, alkoxy, halogen, cyano, substituted alkyl or carboxyl; R5 is preferably H or alkyl; R6 is preferably H, alkyl or aryl and salts and physiologically functional derivatives thereof.
X preferably represents S or NH, A is preferably
and Y preferably represents NH. R1 is preferably COOR8, witn R8 preferably being alkyl or aralkyl. R2 is preferably H or alkyl.
R3 is preferably H, alkoxy or Halo.
4
R is preferably H, alkoxy or halo. R5 is preferably alkyl and R6 is preferably H and salts and physiologically functional derivatives thereof.
Particularly preferred compounds according to the present invention include:
3-Pyridyl 3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylate
[(3-Dimethylamino)phenyl]3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylate
Benzyl 1,3,4-trimethylpyrrolo[3,2-b]carbazole-2-carboxylate
Phenyl 3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylate
3,4-Dimethyl-2-(1-imidazolylcarbonyl)pyrrolo[3,2-b]carbazole
Ethyl 3, 4-dimethylpyrrolo [3,2,-b]carbazole-2-carboxylate;
Ethyl 3,4-dimethylbenzothieno[4,5-f]indole-2-carboxylate;
Benzyl 3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylate;
Benzyl 8-fluoro-3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylate;
Ethyl 8-fluoro-3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylate
Benzyl 3,4,6-trimethylpyrrolo[3,2-b]carbazole-2-carboxylate;
Ethyl 3,4,6-trimethylpyrrolo[3,2-b]carbazole-2-carboxylate;
8-Fluoro-3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylic acid
3,4-Dimethylpyrrolo[3,2-b]carbazole-2-carboxylic acid;
Ethyl 8-methoxy-3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylate;
3,4,6-Trimethylpyrrolo[3,2-b]carbazole-2-carboxylic acid and
Benzyl 8-methoxy-3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylate; and physiologically functional derivatives thereof
Compounds of the general formula (I) have been tested against two specially developed cell lines which are clones of the human fibrosarcoma cell-line, HT1080. One clone, HT1080scc2, retains the transformed phenotype of the parental line, whilst the other, HT10801c, is a morphologically flat, non-tumourigenic, revertant.
Thus, the effects of potential anti-tumour compounds can be evaluated on the basis of their ability to effect detransformation in HT1080scc2 cells.
Compounds of the present invention have been found to. be particularly effective in this assay system.
In addition, compounds of the present invention have been found to be effective against MCF7 human breast cancer cells, A431 Epidermoid carcinoma cells and A285 melanoma cells.
The compounds also exhibit low toxicity against normal cells.
According to a further aspect, the present invention also provides a process for preparing compounds of general formula (I), which process comprises catalysed ring closure of compounds of formula (IV) in the presence of a strong acid.
The present invention also provides for a process for preparing compounds of formula (IV) which process comprises either:
(a) Reaction of a compound of the formula (II) with a compound of the formula (III) to produce a compound of the formula (IV), wherein X,Y,R1,R2,R3 ,R4 and R5 are as defined herein:-
followed by catalysed ring closure.
The reaction is preferably carried out at room temperature in the presence of a strong acid, eg p-toluene sulphonic acid or montmorillonite K10 clay as a catalyst to produce a compound of the invention;
(b) Reaction of a compound of the formula (V) with a compound of the formula (III) to produce a compound of the formula (IV) followed as in (a) by catalysed ring closure.
wherein L is a leaving group. Examples of suitable leaving groups include -OCOCH-, OET, -N+Me3 and halo;
(c) A one step reaction procedure, reacting a compound of the formula (II) with a compound of the formula (III) in the presence of a catalyst, to produce a compound of the invention in a single step. The preferred catalyst is montmorillonite K10 clay;
Insertion of the substituent R1 onto the ring system for example:
(d) Carboxylation of a polyheterocyclic compound using
(i) a carbonyl halide or
(ii) carbon dioxide
According to known procedures (J. March, Advanced Organic Chemistry, 2nd ed, McGraw Hill, New York, 1977, p 497-498).
(e) Alternatively one can produce compounds of the formula (I) wherein R2 is CHO by methods known to those skilled in the art, for example:-
(i) The appropriate aromatic polyheterocycle can be reacted with a formylating agent, such as that generated by the reaction between SnCl4 and Cl2CHOCH3 or equivalent reagents.
For example, according to the method of A. Reiche et al, Chem. Ber. 93, 88 (1960), or with other standard formylating reagents/procedures known in the art, for example, the Gatterman-Koch reaction (CO\HCl\AlCl3\CuCl), the Gatterman reaction (HCN\HCl\ZnCl2), and the Vilsmeier reaction (POCl3\PhN-(Me)CHO or POCl3\Me2NCHO) (J. March, Vide Supra, p 494-497); or
(ii) The appropriate aromatic polyheterocycle, carrying a suitable functional group, said group being converted to an aldehyde group by methods known to those skilled in the art. Suitable functional groups include CHBr2, CH3, COR14, wherein R14 is a primary or secondary C1-6 alkyl group, COOH or a derivative thereof such as an ester, amide, acid chloride or CN; or
(f) Compounds of the formula (I) wherein R1 is CONHR10 may also be produced by the reaction of a compound wherein R1 is COOH or a suitable reactive acid derivative thereof as outlined in J. March,
Vide supra. For example an acid halide can be reacted with a compound NH2R10 in an inert solvent.
(g) Conversion of one compound of formula (I) into another compound of formula (I). Compounds of the invention wherein R1 is COOR8 and R8 is, for example, aralkyl can be converted to free acids wherein R8 is H by reduction in the presence of H2 and a Pd catalyst, or where R8 is, for example, alkyl, by hydrolysis in the presence of an appropriate base e.g. caesium carbonate.
It is thereafter possible for the skilled man to synthesise ester and amide compounds within the scope of the invention by conversion of the free acids obtained, by known procedures. (See J. March, Vide Supra, p363-365).
Compounds of the invention produced as described herein can be converted to other compounds of the invention by electrophilic substitution at R5 and/or R6, to introduce, for example, NO2, halogen and COR13 wherein R13 is as defined herein.
The above processes have been decribed for compounds wherein A is
The skilled man will appreciate that these are equally applicable when A is
, or
In another aspect the invention relates to novel intermediates of the formulae (II), (III), (IV) or (V).
The compounds of the present invention are useful for the treatment of tumours. They may be employed in treating various forms of cancer of mammals including carcinomas, for instance of the stomach, pancreas, breast, uterus and colon; adenocarcinomas, for instance of the lung and colon; sarcomas, for instance fibrosarcoma; leukaemias, for instance lymphocytic leukaemia and lymphomas, for instance myeloid lymphoma.
The invention thus further provides a method for the treatment of tumours in animals, including mammals, especially humans, which comprises the administration of a clinically useful amount of compound of formula (I) or a pharmaceutically acceptable salt or physiologically functional derivative in a pharmaceutically useful form, once or several times a day or in any other appropriate schedule, orally, rectally, parenterally, or applied topically.
In addition, there is provided as a further, or alternative, aspect of the invention, a compound of formula (I) or a pharmaceutically acceptable salt or physiologically functional derivative thereof for use in therapy, for example as an antitumour agent.
The amount of compound of formula (I) required to be effective against the aforementioned tumours will, of course, vary and is ultimately at the discretion of the medical or veterinary practitioner. The factors to be considered include the condition being treated, the route of administration, and nature of the formulation, the mammal's body weight, surface area, age and general condition, and the particular compound to be administered. A suitable effective anti-tumour dose is in the range of about 0.01 to about 100 mg/kg body weight, eg 0.1 to about 100 mg/kg body weight, preferably 1-30 mg/kg body weight. The total daily dose may be given as a single dose, multiple doses, e.g., two to six times per day or by intravenous infusion for selected duration. For example, for a 75 kg mammal, the dose range would be about 8 to 900 mg per day, and a typical dose could be about 50 mg per day. If discrete multiple doses are indicated treatment might
typically be 15 mg of a compound of formula (I) given up to 4 times per day.
Whilst it is possible for the active compound to be administered alone, it is preferable to present the active compound in a pharmaceutical formulation. Formulations of the present invention, for medical use, comprise a compound of formula (I) or a salt thereof together with one or more pharmaceutically acceptable carriers and optionally other therapeutic ingredients. The carrier(s) should be pharmaceutically acceptable in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
The present invention, therefore, further provides a pharmaceutical formulation comprising a compound of formula (I) or a pharmaceutically acceptable salt or physiologically functional derivative thereof together with a pharmaceutically acceptable carrier thereof.
There is also provided a method for the preparation of a pharmaceutical formulation comprising bringing into association a compound of formula (I) or a pharmaceutically acceptable salt or physiologically functional derivative thereof, and a pharmaceutically acceptable carrier thereof.
Formulations according to the present invention include those suitable for oral, topical, rectal or parenteral (including subcutaneous, intramuscular and intravenous) administration. Preferred formulations are those suitable for oral or parenteral administration.
The formulations may conveniently be presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. All methods include the step of bringing the active compound into association with a carrier which constitutes one or more accessory ingredients. In general, the formulations are prepared by uniformly and intimately bringing the active compound into association
with a liquid carrier or a finely divided solid carrier or both and then, if necessary, shaping the product into desired formulations.
Formulations of the present invention suitable for oral administration may be presented as discrete units such as capsules, cachets, tablets or lozenges, each containing a predetermined amount of the active compound; as a powder or granules; or a solution or suspension in an aqueous or non-aqueous liquid such as a syrup, an elixir, an emulsion or a draught.
A tablet may be made by compression or moulding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine the active compound in a free-flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, surface active or dispersing agent. Moulded tablets may be made by moulding in a suitable machine a mixture of the powdered active compound with any suitable carrier.
A syrup may be made by adding the active compound to a concentrated, aqueous solution of a sugar, for example sucrose, to which may also be added any accessory ingredients. Such accessory ingredients(s) may include flavourings, an agent to retard crystallisation of the sugar or an agent to increase the solubility of any other ingredients, such as a polyhydric alcohol for example glycerol or sorbitol.
Formulations for rectal administration may be presented as a suppository with a conventional carrier such as cocoa butter.
Formulations suitable for parenteral administration conveniently •comprise a sterile aqueous preparation of the active compound which is preferably isotonic with the blood of the recipient. Such formulations suitably comprise a solution of a pharmaceutically and pharmacologically acceptable acid addition salt of a compound of the formula (I) that is isotonic with the blood of the recipient.
Useful formulations also comprise concentrated solutions or solids containing the compound of formula (I) which upon dilution with an appropriate solvent give a solution for parenteral administration as above.
In addition to the aforementioned ingredients, the formulations of this invention may further include one or more accessory ingredient(s) selected from diluents, buffers, flavouring agents, binders, surface active agents, thickeners, lubricants, preservatives (including antioxidants) and the like.
In a further aspect the present invention provides the use of a compound of formula (I) or a pharmaceutically acceptable salt or physiologically functional derivative thereof for the manufacture of a medicament for the treatment of tumours.
The invention will now be illustrated by the following non-limiting Examples: All temperatures are in degrees Celcius (ºC)
IR spectra were recorded on a Perkin-Elmer 257 grating spectrophotometer or a Bruker FS66 spectrophotometer.
U.V. spectra were measured in ethanol on a Unicam SP800 spectrophotometer.
1H NMR spectra were obtained on a Bruker WM 360-NMR spectrophotometer at 360 MHz, or on a Bruker AC200 spectrophotometer at 200 MHz. J values are given in Hz.
Mass spectra were obtained on Varian CH5D(EI), Kratos Concept (El) or Kratos Ms50(FAB) instruments.
Example 1
Preparation of Intermediates
Preparation of Pyrroles
Ethyl 4-acety1-3,5-dimethylpyrrole-2-carboxylate, benzyl 4-acetyl-3,5-dimethylpyrrole-2-carboxylate and ethyl 4-acetyl-3-ethyl-5-methylpyrrole-2-carboxylate) were prepared according to the method of A.W.Johnson et al, J. Chem. Soc., 4254 (1958).
N-Methylation of Pyrroles - General Procedure
A mixture of the pyrrole (20 mmol) methyl iodide (50 mmol) and potassium carbonate (50 mmol) was heated to reflux in methyl ethyl ketone (50 ml) for 8 h. If TLC (toluene/ethyl acetate 3:1) indicated incomplete reaction, further aliquots of methyl iodide (50 mmol) and potassium carbonate (50 mmol) were added and the mixture heated to reflux for a further 6 h. After evaporation in vacuo to dryness, the residue was taken up in warm water and extracted with ethyl acetate (3×50 ml). The combined extracts were dried over magnesium sulphate and evaporated in vacuo to leave a yellow oil or solid which was crystallised from aqueous ethanol.
Ethyl 4-acetyl-1,3,5-trimethylpyrrole-2-carboxylate
Obtained from ethyl 4-acetyl-3,5-dimethylpyrrole-2-carboxylate as white crystals (2g; 41%) m.p. 61-62°C (Found: C, 64.17; H, 7.82; N
6.16 C12H17NO3 requires C, 64.55; H, 7.68; N, 6.27%) δH([2H6]-DMSO)
4.25 (2H, q, CH2CH3), 3.70 (3H, s, 1-CH3), 2.43 and 2.42 (2 × 3H, 2 × s, 3-CH and COCH3), 2.38 (3H, s, 5-CH3) and 1.29 (3H, t, CH2CH3); m/z
(%) 224(MH+, 100), 208(40), 194(20), 178(40) and 133(20) (FAB); ʋmax
(KBr Disc)/CM-1 2984, 1691 and 1651.
Benzyl 4-acetyl-1,3,5-trimethylpyrrole-2-carboxylate
Obtained from benzyl 4-acetyl-3,5-dimethyl-2-carboxylate as white crystals m.p. 78-79°C (Found: C, 71.30; H, 6.74; N, 4.79; C17H19NO3 requires C, 71.56; H, 6.71; N, 4.91%); δH ([2H6]-DMSO) 7.52-7.27 (5H, m, ArH), 5.30 (2H, s, CH2Ph), 3.73 (3H, s, 1-CH3), 2.42 (6H, s, 3-CH3 and COCH3) and 2.38 (3H, s, 5-CH3); m/z (%) 285(76, M+), 270(87), 194 (53), 178(23), 151(36), 136(26) and 91(100); ʋmax (KBr Disc)/cm-1
2974, 1693 and 1641.
Preparation of the 5-Acetoxymethyl-4-acetylpyrroles - General procedure. To a cooled (0ºC) and stirred suspension of the 4-acetyl-5-methylpyrrole (0.02 mol) in dry diethyl ether (20 cm3) was added, dropwise over 15 min, freshly distilled sulfuryl chloride (2.2 cm3,
1.25 equiv.). The reaction mixture was stirred further and the chloromethyl derivative crystallised out slowly, filtration gave the
5-chloromethyl derivative as colourless crystals. The purity of the chloromethylpyrrole was checked by 1H NMR spectroscopy (90 MHz) and it was used directly without recrystallisation.
The above chloromethylpyrrole (0.01 mol) was added to a solution of sodium acetate (3 g) in acetic acid (50 cm3), the mixture stirred for
2 h and poured into ice-water (200 cm3). The resulting solid was washed well with water until acid-free before drying.
Ethyl 5-acetoxymethyl-4-acetyl-3-methylpyrrole-2-carboxylate
crystallised from benzene as colourless needles (1.87 g, 70%) m.p.
135.5-138 °C (Found: C, 58.6; H, 6.45; N, 5.15.C13H17NO5 requires C,
58.4; H, 6.41; N, 5.24%); δH(CDCl3) 9.57 (1 H, br s, NH), 5.40 (2 H, S, CH2OAc), 4.35 (2 H, q, OCH2CH3), 2.6 (3H, s, 3-CH ) 2.5 (3 H, s,
COCH3), 2.17 (3H, s, OCOCH3) and 1.4 (3H, t, OCH2CH3); m/z (%) 267(83,M+), 224(46), 207(27), 178(100) and 162(42).
Benzyl 5-acetoxymethyl-4-acetyl-3-methylpyrrole-2-carboxylate crystallised from methanol as colourless needles (2.34 g, 71%) m.p.
138-141 °C (Found: C, 65.8; H, 5.95; N, 4.3 C18H19NO5 requires c,
65.64; H, 5.81; N, 4.25%); δH(CDCl,) 9.44 (1 H, br s NH), 7.49-7.32 (5 H,m,ArH), 5.40 (2H,s,CH OAc), 5.35 (2H,s,CH Ph), 2.62 (3H,s,3-CH3), 2.49 (3H,s,CH CO) and 2.14 (3H,s,OCOCH ); m/z (%) 329 (9,M ), 286 (13), 269(4), 178(19) and 91(100).
In the case of benzyl 5-acetoxymethyl-4-acetyl-3-(2-methoxycarbonylethyl)-pyrrole-2-carboxylate, there was no precipitation when the solution was poured into ice-water. Extraction with chloroform (3 × 100 cm3), drying and removal of solvent under reduced pressure gave an oil which was crystallised from benzene-light petroleum to yield colourless needles (2.69g, 67%) m.p. 97-100 C (Found C, 62.9; H 5.9; N 3.45. C H NO requires C,62.8; H, 5.78; N, 3.49%); δH(CDCl3) 9.15 (1H, br s,NH), 7.50-7.30 (5H, m, ArH), 5.35 (4 H, 8, CH Ph and CH2OAc), 3.63 (3 H, s, OCH3), 3.37 (2 H, t, CH2CH2CO), 2.58 (2 H, t, CH2CO), 2.51 (3 H,s,COCH3) and 2.15 (3 H,s,OCOCH3); m/z(%) 401(4,M+), 341(8), 268(6),250(60) and 91(100).
Ethyl 5-acetoxymethyl-4-acetyl-1,3-dimethylpyrrole-2-carboxylate Crystallised from ethyl acetate/cyclohexane (61%) m.p. 100-101ºC.
(Found: C, 59.38; H, 6.73; N, 4.95. C14H19NO5 requires C, 59.78; H,
6.81; N, 4.98%); δH([ H-]-DMSO) 5.30 (2H, s, CH2OAc), 4.29 (2H, q,
CH2CH3), 3.77 (3H, s, N-CH3), 2.43 and 2.42 (2 × 3H, 2 × s, 3-CH3 and
CH3CO), 2.02 (3H, s, OCOCH3) and 1.31 (3H, t, CH2CH3); m/z (%) 281
(34,M+), 238(100), 222(48) and 192(52); ʋmax (KBr Disc)/cm-1 1712 and
1697.
Benzyl 5-acetoxymethyl-4-acethl-l.3-dimethylpyrrole-2-carboxylate
Crystallised from ethyl acetate/cyclohexane. δH (2[H]6-DMSO)
7.51-7.32 (5H, m, ArH), 5.34 and 5.32 (2 × 2H, 2 × s, CH2Ph and
CH2OAc), 3.78 (3H, s, N-CH3), 2.46 and 2.45 (2 × 3H, 2 × s, 3-CH3 and
CH3CO) and 2.04 (3H, s, OCOCH3) ; m/z (%) 343(5, M+), 284(100) and 91 (95).
Ethyl 5-acetoxymethyl-4-acetyl-3-ethylpyrrole-2-carboxylate Crystallised from ether/petrol as fawn needles (61%) with m.p. 97-98ºC
(Found: C, 59.44; H, 6.78; N, 4.80. C14H19NO5 requires: C, 59.76; H,
6.81; N, 4.98%); δH (CDCl3) 9.40 (1H, br 8, 1-NH), 5.38 (2H, s,
CH2OAc), 4.38 (2H, q, J 7, CO2CH2), 3.10 (2H, q, J 7.5, 3-cH2), 2.54
(3H, s, COCH3), 2.18 (3H, s, OCOCH3), 1.40 (3H, t, J 7.5, CO2CH2CH3),
1.23 (3H, t, J 7.5, 3-CH2CH3); m/z (%) 281 (42, M+), 238(61), 221(89),
206(58), 192(92), 175(95), 160(81), 147(59), 43(100); ʋmax (KBr disc)/cm 3277, 1738, 1674, 1657.
Synthesis of the 3-(Pyrrolylmethyl)indole, 2- (Pyrrolylmethyl)benzofuran and 3-(Pyrrolylmethyl)benzothiophene - General procedure.
A solution of the 5-acetoxymethyl-4-acetylpyrrole (1.0 mmol) and indole (1.0 mmol) in 1,2-dichloroethane (10 cm ) was heated at gentle reflux and stirred with Montmorillonite clay (1 g) for 1.5-2 h. After filtration from clay and washing well with 1,2-dichloroethane, evaporation of the combined filtrates under reduced pressure gave an oil. This oil was submitted to flash chromatography on silica, eluting with ethyl acetate in light petroleum to give 3-(3'-acetyl-5'-ethoxycarbonyl-4'-methylpyrrol-2'-ylmethyl) indole. It gave colourless crystals from ethyl acetate-light petroleum (0.1465 g, 45%). m.p.
180-182°C (Found: C,70.5; H, 6.25; N, 8.65. C19H20N2O3 requires C,
70.4; H, 6.21; N, 8.64%); δH(CDCl3) 8.78 (1H, s, pyr-NH), 8.27 (1 H, s, ind-NH), 7.45 (1H, d, J7,4-H) 7.42 (1H, d, J7, 7-H), 7.25 (1H, t, J7, 6-H), 7.14 (1H, t, J7, 5-H), 7.10 (1H, s, 2-H), 4.45 (2H, s, 3-CH ), 4.22 (2H, q, OCH2CH3), 2.63 (3H, s, 4'-CH ) 2.53 (3H, S, CH CO) and 1.25 (3 H, t, OCH2CH3) ; m/z (%) 324(100,M+) 309(48),
277(25), 263(54), 250(38),235(30),207(48), 139(24), 130(30), 117(67) and
90(16); ʋmax(CHCl3)/cm-1 3490, 3430, 1680 and 1650.
Benzofuran (1.0 mmol) when used instead of indole, after chromatography, gave 2-(3'-acetyl-5'-ethoxycarbonyl-4'-methylpyrrol-2'-ylmethyl)benzofuran (0.106 g, 32.6%), m.p. 124-127ºC (Found: C, 70.1;
H, 6.1; N, 4.15 C19H19NO4 requires C, 70.14; H, 5.89; N, 4.31%); δH(CDCl3) 9.25 (1 H, s, NH), 7.50(1 H, d, J7.3, 4-H) 7.44 (1H, d,
J7.3, 7-H) 7.28-7.18 (2 H, m, 6-H and 5-H), 6.57 (1 H, 8, 3-H), 4.50
(2 H, S, 2-CH ), 4.31 (2 H, q, OCH2CH3) 2.62 (3 H, s, 4'-CH ) 2.50 (3
H, s, CH3CO) and 1.35 (3 H, t, OCH2CH3); saturation of the singlet 3-H at δ 6.51 enhanced the signals due to 4H at 07.50 (2.7%) and 2-CH2 at δ 4.50 (0.8%); m/z (%) 325(100,M+), 310(4),279(29), 264(17), 251(59),
236(27), 208(19), 193(9), 131(7) and 118(7); and the 2,3-bis
(3 '-acetyl-5'-ethoxycarbony1-4'-methylpyrrol-2'-ylmethyl)benzofuran
(0.0238 g, 8.94%) m.p. 255-257°C; δH (CDCl3) 10.09 (1 H, 8, NH) 9.95
(1H, s, NH), 7.32 (1H, d, J7.7, 4-H), 7.27 (1 H, d, J7.7, 7-H), 7.17 (1 H, t, J7.7, 6-H), 7.08 (1 H, t, J7.7, 5-H) 4.45 (2 H, s, 2-CH2), 4.40 (2 H, 8, 3-CH2), 4.36 (2H, q, OCH2CH3), 4.27 (2 H,q,OCH2CH3), 2.64(3 H,s,4'-CH3), 2.63(3H,s,4'-CH3), 2.58 (3H, s, CH3CO), 2.54 (3 H, s, CH3CO), 1.39 (3H, t, OCH2CH3) and 1.31 (3 H, t, OCH2CH3); m/z (%) 532(11,M+), 490(24), 444(9), 397(6), 324(100), 282(18), 278(27), 236(20), 209(28) and 162(28) (Found: M+, 532.2210. C30H32N2O7 requires M, 532.2209).
When benzothiophene (1.0 mmol) was used in the same way as indole, chromatography using ethyl acetate in dichloromethane as eluent gave colourless crystals of 3-(3'-acetyl-5'-ethoxycarbonyl-4'-methylpyrrol-2'-ylmethyl)benzothiophene (0.0963g, 28.2%) m.p.125-128°C (Found: C, 6675; H, 5.8; N,4.1 C19H19NO3S requires C, 66.84; H, 5.61;
N, 4.10%); δH(CDCl3) 8.72 (1 H, br, s, NH), 7.88 (1 H, m, 4-H), 7.63
(1 H, m, 7-H), 7.37 (2 H, m, 6-H and 5-H), 7.20 (1 H, 8, 2-H), 4.54 (2 H, s, 3-CH2), 4.23 (2 H, q, OCH2CH3), 2.62 (3 H, 8, 4'-CH3), 2.53 (3 H, s, CH3CO) and 1.28 (3 H, t, OCH CH ) ; saturation of the 3-CH2 protons at δ 4.54 enhanced the signals due to NH at δ 8.72 (3.3%), 4-H
at δ 7.88 (7.7%), 2-H at δ 7.20(6%) and CH3CO at δ 2.53 (1.3%); m/z
(%) 341(100,M+), 326(9), 298(6), 295(20), 230(39), 267(46), 252(32),
224(27), 194(26) and 148(22); and the 2,3-bis (3'-acetyl-5'-ethoxycarbonyl-4'-methylpyrrol-2'-ylmethyl)benzothiophene as a pale yellow solid (0.0264 g, 9.6%), m.p. 206-209°C (Found: C, 65.6; H, 5.8; N 5.1
C30H32N2O6S requires C, 65.67; H, 5.88; N, 5.11%), δH(CDCl3) 9.77 (1H, br, s, NH), 9.43 (1H, br, s, NH), 7.70 (1 H, m, 4-H), 7.49(1 H, m, 7-H), 7.26 (2 H, m, 6-H and 5-H), 4.55 (2 H, s, CH2), 4.53(2 H, s, CH2), 4.32 (2 H, q, OCH2CH3), 4.24 (2 H, q, OCH2CH3), 2.61 (3 H, s, 4'-CH3), 2.60 (3 H, 8, 4'-CH3), 2.57 (3H, S, CH3CO) 2.49 (3 H, s, CH3CO), 1.35 (3H,t,OCH2CH3) and 1.28 (3H H,t,OCH2CH3) ;m/z(%) 548(5,M+), 530(11), 340(100), 294(27) and 162(10).
Example 2
Synthesis of 3-(Pyrrolylmethyl)benzothiophenes and 3-(Pyrrolylmethyl)indoles a) 3-(3'-Acetyl-5'-benzyloxycarbonyl-4'-methylpyrrol-2'-ylmethyl) benzothiophene
A solution of the 5-acetoxymethyl-4-acetylpyrrole (0.33g; 1.0 mmol) and benzothiophene (0.14 g; 1.05 mmol) in 1,2-dichloroethane (10 cm ) was heated at reflux and stirred with Montmorillonite K10 clay (1 g) for 2.5 h. After cooling and filtration from the clay, which was washed well with 1,2-dichlorethane, the combined filtrates were evaporated under reduced pressure to leave a yellow oil. Flash chromatography on silica, eluting with diethyl ether/light petroleum ( 1:2 ) gave the title compound as a colourless solid. δH(CDCl3) 8.72 (1H, s, NH), 7.92-7.84 (1H, m, 4-H), 7.69-7.58
(1H, m, 7-H), 7.43-7.16 (8H, m, 2-H, 5-H, 6-H, ArH), 5.23 (2H, 8, CH2Ph), 4.50 (2H, s, 3-CH2), 2.61 (3H, s, 4'-CH3) and 2.50
(3H, s, CH CO); m/z (%) 403 (M+, 100); ʋmax (KBr Disc) /cm-1
3290, 1690 and 1659. b) 3-(3'-Acetyl-5'-ethoxycarbonyl-4'-methylpyrrol-2'-ylmethyl)-5- cyanoindole
A solution of the 5-acetoxymethyl-4-acetylpyrrole (0.7 g, 2.6 mmol) and 5-cyanoindole (0.41 g, 2.9 mmol) in 1,2-dichloroethane (50 cm ) was heated at reflux and stirred with Montmorillonite K10 clay (2.1 g) for 6 h. After cooling and filtration from the clay, which was washed well with 1,2-dichloroethane, the combined filtrates were evaporated under reduced pressure to leave an orange solid. Crystallisation from dichloromethane/ ethyl acetate yielded a small amount of analytically pure title compound as cream crystals. The evaporated mother liquors were submitted to flash chromatography on silica, eluting with dichloromethane/ethyl acetate (9:1) to give further product (0.65g, 71%). m.p. 213-214°C (Found: C, 68.60; H, 5.46; N, 11.99. C20H19N3O3 requires C, 68.75; H, 5.48; N, 12.03%) δH([2H6]-DMSO) 12.05 (1H, s, 1'-NH), 11.38 (1H, s, 1-NH), 8.20 (1H, m, 4-H), 7.50 (1H, dd, J0.7 and 8.5, 7-H), 7.39 (1H, dd, J1.7 and 8.5, 6-H), 7.18 (1H, s, 2-H), 4.32 (2H, s, 3-CH 4.27 (2H, q, CH2CH3), 2.33 (3H, s, 4'-CH3), 2.51 (3H, s, CH3CO) and
1.30 (3H, t, CH2CH3); m/z (%) 350(M+, 70), 302(16), 279(18), 237(35), 208(100) and 181(20); ʋmax (KBr Disc)/cm -1 3309, 2218 and 1665. c) 3-(3'-Acetyl-5'-ethoxycarbonyl-4'-methylpyrrol-2'-ylmethyl)
indole-5-carboxylic acid
A solution of the 5-acetoxymethyl-4-acetylpyrrole (0.74g, 2.8 mmol) and indole-5-carboxylic acid (0.5g, 3 mmol) in toluene (50 cm3) was stirred at room temperature with Montmorillonite K10 clay (1 g) for 10 days. After cooling and filtration from the clay, which was washed well with toluene, the combined filtrates
were evaporated under reduced pressure to leave an orange solid.
Crystallisation from ethyl acetate/cyclohexane yielded the title compound as a grey powder (0.15g, 15%). m.p. 227-228ºC (Found:
C, 64.96; H, 5.58; N, 7.34. C20H20N2O5 requires C, 65-21; H,
5.47; N, 7.60%); δH([2H6]-DMSO) 12.35 (1H, br, CO2H) 12.04 (1H, s, 1'-NH), 11.17 (1H, s, 1-NH), 8.31 (1H, d, J1.6, 4-H), 7.70
(1H, dd, J1.6 and 8.7, 6-H), 7.37 (1H, d, J8.7, 7-H), 6.98 (1H, s, 2-H), 4.36 (2H, 8, 3-CH2), 4.26 (2H, q, CH2CH3), 2.51 and
2.31 (2 × 3H, 2 × s, 4'-CH and CH3CO) and 1.29 (3H, t,CH2CH3); m/z (%) 369(22(M+1)+), 351(37), 323(18), 305(19), 232(19),
208(60) and 181(20), 162(100); ʋmax(KBr Disc) /cm-1 3359 and
1676. d) 3-(3'-Acetyl-5'-ethoxycarbonyl-4'-methylpyrrol-2'-ylmethyl)-5- bromoindole
A solution of the 5-acetoxymethyl-4-acetylpyrrole (1.3g, 4.9 mmol) and 5-bromoindole (1.09g, 5.6 mmol) in 1,2-dichloroethane (100 cm3) was heated at reflux and stirred with Montmorillonite K10 clay (3 g) for 5 h. After cooling and filtration from the clay, which was washed well with 1,2-dichloroethane, the combined filtrates were evaporated under reduced pressure to leave a yellow solid. Crystallisation from dichloromethane/ light petroleum/acetone yielded the title compound as cream crystals (0.33 g, 17%). m.p. 181-183 C (Found: C, 56.24; H, 4.70; N, 6.86. C19H19BrN2O3 requires C, 56.59; H, 4.75; N,
6.95%); δH([2H6]-DMSO) 12.00 (1H, s, 1'-NH), 11.00 (1H, s, l-NH), 7.82 (1H, d, J1.9, 4-H), 7.31 (1H, d, J8.6, 7-H), 7.16
(1H, dd, J1.9 and 8.6, 6-H), 7.02 (1H, s, 2-H), 4.30 (2H, s,
3-CH ), 4.28 (2H, q, CUHU2CH3),2.51 and 2.33 (2 × 3H, 2 × S,
4'-CH and CH3CO) 1.31 (3H, t, CH2CH3) m/z (%) 404 and 402(100
M+), 389 and 387(24), 357(24), 330 and 328(32), 206(36) and
178 (26) ; ʋmax (KBr Disc ) /cm-1 3373 and 1672.
Example 3 a) Ethyl 3,4-dimethylPyrrolo[3,2,-b]carbazole-2-carboxylate
A solution of the 3-(pyrrolylmethyl)indole (0.108 g 0.33 mmol) was heated at gentle reflux in 1,2-dichloroethane (10 cm3) and stirred with Montmorillonite K10 clay (1 g) for 2 h. TLC then showed a single compound had been formed and that reaction was complete. After filtration from clay and washing well with
1,2-dichloroethane, evaporation of the combined filtrates under reduced pressure gave a yellow solid which crystallised from ethyl acetate to give the pyrrolo[3,2-b]-carbazole as yellow crystals (0.076 g; 75%), m.p. 209.5-211ºC (Found: C, 74.6; H,
6.14; N, 9.03. C19H18N2O2 requires C.74.5; H, 5.92; N, 9.14%); δH[2H6-J-DMSO) 11.22 (1H, s, 1-NH), 10.70 (1 H, 8, 5-NH), 8.06
(1H, d, J7, 9-H), 7.85 (1H, s, 10-H), 7.40 (1H, d, J7, 6-H),
7.35 (1H, t, J7, 7-H), 7.08 (1H, t, J7, 8-H), 4.35 (2H, q,
OCg CH ) 2.91 and 2.90 (2 × 3H, 2 × S, 3-CH and 4-CH and 1.35
(3 H, t, OCH CH ) . Saturation of the 10-H at δ 7.85 enhanced the singlets due to 1-NH at δ 11.22 (3%) and 9-H at δ 8.06 (4%); m/z(%) 306(56,M+), 260(100), 323(39), 205(15), 140(18) and
130(26); ʋmax ( CHCl3) /cm-1 3480 and 1700; λmax (EtOH) /nm 226 , 268 ,
310sh, 327sh, 340, 390 and 410sh. b) Ethyl 3,4-dimethyl-1H-benzofuro[3,2,-f]indole-2-carboxylate Toluene-p-sulfonic acid (50 mg) was added to a solution of the
2-(pyrrolylmethyl)benzofuran (0.100 g 0.31 mmol) in toluene (10 cm3 ), and the reaction mixture was heated under reflux for 3 h.
On cooling, the product crystallised out, and after filtration and washing with ethanol gave the title compound as pale yellow crystals (0.084 g, 88.8%), m.p. 262-265ºC (Found: C, 74.25; H, 5.55; N, 4.6 C19H17NO3 requires C. 74.25; H, 5.56; N, 4.56%);
δH([2H6]-DMSO) 11.52 (1 H, s, 1-NH). 8.18 (1 H, d, J7.5, 5-H),
7.62 (1 H, d, J7.5, 8-H), 7.46 (1 H, t, J7.5, 7-H), 7.38 (1H, t,
J7.5, 6-H) 7.38 (1 H, s, 10-H), 4.37 (2 H, q, OCH2CH3), 3.14 (3
H, s, 4-CH3), 2.91 (3 H, s, 3-CH3) and 1.39 (3 H, t, OCH2CH3); saturation .of the 4-CH3 at δ 3.14 enhanced the signals due to
5-H at δ 8.18 (4.5%) and 3-CH3 at δ 2.91 (2.6%); m/z (%) 307
(53,M+), 261(100), 233(31) and 205(9); ʋmax(Nujol)/cm-1 3350 and
1686; λmax(EtOH)/nm 240, 269,293,330 and 344.
c) Ethyl 3,4-dimethyl-1H-[1]benzothieno[2,3-f]indole-2-carboxylate
Toluene-p-sulfonic acid (45 mg) was added to the solution of the 3-(pyrrolylmethyl)benzothiophene (0.100 g, 0.29 mmol) in toluene (10 cm ) and the reaction mixture was heated under reflux for 3 h. Evaporation of the solvent and washing the resulting solid with ethanol gave the title compound as a pale yellow solid (0.0758 g, 80%), m.p. 191-193 °C (Found: C,70.3; H 5.5; N, 4.2.
C19H17NO2S requires C, 70.6; H, 5.30; N, 4.33%); δH( [2H6]-DMSO)
11.64 (1 H, s, NH), 8.25(1 H, m, 9-H), 8.12 (1 H, s, 10-H) 7.95 (1 H, m, 6-H), 7.48 (2 H, m, 7-H and 8-H), 4.38 (2 H, q,
OCH2CH3), 2.87 and 2.85 (2 × 3H, 2 × S, 3-CH3) and 1.37 (3 H, t, OCH2CH3); m/z (%) 323(53,M+), 277(100), 249(33), 221(15), 139(7) and 111(11); ʋmax(Nujol)/cm-1 3350 and 1686; λmax(EtOH)/ nm
240,269,293,330 and 344. d) Benzyl 3,4-dimethyl-1H-[1]benzothieno[2,3-f]indole-2-carboxylate
Toluene-p-sulphonic acid (40 mg) was added to the solution of the 3-(pyrrolylmethyl)benzothiophene (0.100 g, 0.25 mmol) in toluene (12 cm3 ) and the reaction heated under reflux for 6 h.
Evaporation of the solvent and washing the resultant solid with ethanol gave the title compound as a pale yellow solid (0.02 g,
20%) m.p. 203-204°C (Found: C, 74.7; H, 4.9; N, 3.6; C24H19NO2S requires C, 74.8; H, 5.0; N, 3.6%); δH ([2H6]-DMSO) 11.64 (1H,
s , NH) 8.27-8.15 ( 1H, m, 9-H) , 8.10 ( 1H, s , 10-H) , 7.90-7.89 (1H, m, 6-H) . 7.60-7.30 (7H, m, 7-H, 8-H, ArH) , 5.40 (2H, s ,
CH ) and 2.87 ( 6H, s, 2 × CH3) ; m/z ( % ) 385 ( 100 , M ) , 277 ( 89 ) , 248(25), 221(15) and 91(28); ʋmax (KBr Disc/cm-13331 and 1672. e) Ethyl 8-cyano-3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylate
A mixture of the 3-(pyrrolylmethyl) indole (0.6 g, 1.7 mmol) and Montmorillonite K10 clay (2 g) in toluene was stirred and heated at reflux for 24 h. After cooling and filtration from the clay, which was washed well with toluene, the combined filtrates were evaporated under reduced pressure to leave a brown solid which was submitted to flash chromatography on silica, eluting with eyelohexane/ethyl acetate (3:1) to give a yellow solid.
Crystallisation from cyclohexane/ethyl acetate yielded the title compound as a yellow powder (0.030 g, 5%). m.p. >240ºC (Found:
C, 71.54; H, 5.18; N, 12.78, C H N O .0.2H O requires C,
71.71; H, 5.24; N, 12.54%) δH([2H6]-DMSO) 11.39 (1H, s, 1-NH),
11.29 (1H, s, 5-NH), 8.65 (1H, d, J1.7, 9-H), 8.01 (1H, s,
10-H), 7.71 (1H, dd, J1.7 and 8.6, 7-H), 7.50 (1H, d, J8.6,
6-H), 4.36 (2H, q, CH2CH3), 2.91 and 2.89 (2 × 3H, 2 × s, 4-CH3 and 3-CH3) and 1.38 (3H, t, CH2CH3); m/z (%) 331 (52,M+)
285(100), 256(32), 229(12), 167(14) and 149(40); ʋmax(KBr
Disc)/cm-13414, 3550, 2212 and 1664. f) 3,4-Dimethyl-2-ethoxycarbonylpyrrolo[3,2-b]carbazole-8- carboxylic acid
A mixture of the 3-(pyrrolylmethyl)indole (O.lg, 0.3 mmol) and Montmorillonite K10 clay (0.34 g) in toluene (15 cm3 ) was stirred and heated at reflux for 6 h. After cooling and filtration from the clay, which was washed well with toluene, the combined filtrates were evaporated under reduced pressure to leave an orange solid. Crystallisation from methanol/dichloromethane yielded the title compound as a yellow powder (0.027 g,
28% ) . m.p. >260°C (Found: C, 68.12 ; H, 5.19; N, 7.91
C20H18N2O4.0. 05H2O requires C, 68.39; H, 5.19; N, 7.98%); δH
(2[H]6-DMSO) 12.42 (1H, br, COOH), 11.30 (1H, s, 1-NH), 11.09
(1H, s, 5-NH), 8.68 (1H, s, 10-H), 8.07-7.93 (2H, m, 7-H and
9-H), 7.45 (1H, d, J9, 6-H), 4.38 (2H, q, CH3CH3), 2.94 and 2.90
(2 × 3H, 2 × s, 4-CH and 3-CH3) and 1.39 (3H, t, CH2CH3); m/z
(%) 350(100, M+), 304(100), 278(40), 232(35) and 181(38); ʋmax
(KBr Disc)/cm-1 3459, 1697 and 1674.
Ethyl 8-bromo-3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylate
A mixture of the 3-(pyrrolylmethyl) indole (0.3g, 0.74 mmol) and Montmorillonite K10 clay (1 g) in toluene was stirred and heated at reflux for 6 h. After cooling and filtration from the clay, which was washed well with toluene, the combined filtrates were evaporated under reduced pressure to leave a brown solid which was submitted to flash chromatography on silica, eluting with dichloromethane/light petroleum (7:3) to give a yellow solid.
Crystallisation from cyclohexane/ethyl acetate yielded the title compound as a yellow powder (0.070 g, 24%). m.p. 204-205ºC
(decomp.) (Found: C,59.17; H, 4.43; N, 7.30. C19H17Brn2O2 requires C, 59.23; H, 4.45; N, 7.27%); δH([2H6]-DMSO) 11.26 (1H, s, 1-NH), 10.79 (1H, 8, 5-NH), 8.30 (1H, d, J2.2, 9-H), 7.92
(1H, s, 10-H), 7.47 (1H, dd, J2.2 and 8.8, 7-H), 7.35 (1H, d,
J8.8, 6-H), 4.36 (2H, q, CH2CH3), 2.89 and 2.88 (2 × 3H, 2 × s,
4-CH and 3-CH3) and 1.38 (3H, t, CH2CH3); m/z (%) 386 and
384(100, M+), 340 and 338(70), 232(60) and 181(50); ʋmax(KBr
Disc)/cm-13350, 1705 and 1663.
Example 4
One-pot Synthesis of the Pyrrolocarbazoles - General procedure.
A solution of indole (1.0 mmol) and the 5-acetoxymethyl-4-acetylpyrrole (1.0 mmol) in 1,2-dichloroethane (10 cm3) was heated under gentle reflux and stirred with Montomorillonite K10 clay (1 g) for 3-4 h. The colour of clay turned light brown and the reaction was followed to completion by TLC. After filtration from clay and washing well with 1,2-dichloroethane, evaporation of the combined filtrates gave the pyrrolo[3,2-b]carbazoles which were obtained as yellow crystals after crystallisation from dichloromethane or ethyl acetate. a) Ethyl 3,4-dimethylpyrrolo[3,2,-b]carbazole-2-carboxylate
(0.199 g, 65%) was obtained from the reaction of indole and the 5-acetoxymethyl-4-acetylpyrrole. It was identical in all respects to the pyrrolo[3,2-b]carbazole from example 1. b) Benzyl 3,4-dimethylpyrrolo[3.2-b]carbazole-2-carboxylate
(0.179 g, 48.8%) was obtained from the reaction between indole and the 5-acetoxymethyl-4-acetylpyrrole, it had m.p. 229-232ºC
(Found: C, 78.2; H, 5.65; N, 7.8. C24H20N2O2 requires C, 78.23;
H, 5.47; N,7.60%); δH[2H6-]-DMSO-d6) 11.29 (1 H, s, 1-NH), 10.65
(1 H, s, 5-NH),8.08 (1 H, d, J8, 9-H), 7.89 (1 H, s, 10-H), 7.56-7.34 (7 H, m, ArH, 6-H and 7-H), 7.08 (1 H, t, J7, 8-H), 5.42 (2 H, s, CH2Ph) and 2.92 (6H, s, 3-CH3 and 4-CH3); m/z (%) 368(74,M+), 354(10), 260(100), 246(13), 231(20) and 91(31).
The pyrrolo[3,2-b]carbazole (0.166 g, 45%) was also obtained from the reaction of indole and the 4-acetyl-5-(ethoxymethyl)- pyrrole.
c) Ethyl 8-methoxy-3.4-dimethylpyrrolo[3.2-b]carbazole-2-carboxylate
Was obtained from the reaction of 5-methoxyindole and the 5-acetoxymethyl-4-acetyl pyrrole, it had m.p. 119-122ºC (Found:
C,71.6; H, 6.0; N, 8.05. C20H20N2O3 requires C, 71.4; H, 5.99;
N, 8.33%); (δH([2H -DMSO) 11.20 (1 H, s, 1-NH), 10.38 (1 H, 8,
5-NH), 7.85(1 H, s, 10-H), 7.62 (1 H, d, J 2.5, 9-H), 7.31 (1 H, d, J9, 6-H), 7.01 (1 H, dd, J9 and 2.5, 7-H), 4.38 (2 H, q, OCH CH ), 3.88 (3 H, 8, OCH ), 2.89 and 2.87 (2 × 3H, 2 × S, 3-CH3 and 4-CH3) and 1.39 (3 H, t, OCH2CH3); m/z (%) 336(60,M+), 290(100), 275(5), 262(4), 247(23), 219(8) and 145(9). d) Benzyl 8-methoxy-3,4-dimethylpyrrolo[3.2-b]carbazole-2-carboxylate
(0.139 g, 35%) was obtained from the reaction of 5-methoxy indole and the 5-acetoxymethyl-4-acetylpyrrole, it had m.p.
212-215°C (Found: C, 75.4; H, 5.55; N, 6.95. C25H22N2O3 requires
C, 75.4; H, 5.57; N, 7.03%); δH([2H6]-DMSO) 11.29 (1 H, s,
1-NH), 10.38 (1H ,s,5-NH), 7.88 (1 H, 8, 10-H), 7.65 (1 H, d, J2.5, 9-H), 7.58-7.36 (5H, m, ArH), 7.32 (1H, d, J9, 6-H), 7.02 (1 H, dd, J9 and 2.5, 7-H), 5.43 (2 H, s, CH3Pn), 3.88 (3 H, s, OCH3), 2.92 (3 H, a, 4-CH3) and 2.89 (3H, s, 3-CH3); m/z (%) 398(73,M+), 290(100), 262(10), 247(15), 219(7) and 91(17). e) Ethyl 8-fluoro-3,4-dimethylpyrrolo[3.2-b]carbazole-2-carboxylate
(0.131 g, 40.5%) was obtained from the reaction of 5-fluoroindole and the 5-acetoxymethyl-4-acetylpyrrole, it had m.p.
231-234°C (Found: C, 70.5; H, 5.3; N, 8.4. C19H17FN2O2 requires
C, 70.4;,H, 5.28; N, 8.64%); δH[2H6]-DMSO) 11.27 (1 H, s, 1-NH),
10.64 (1H, 8, 5-NH), 7.93 (1 H, dd, J9 and 2.5, 9-H), 7.88 (1 H, s, 10-H), 7.36(1 H, dd, J9 and 6,6-H), 7.19 (1 H, dt, J9 and 2.5, 7-H), 4.36 (2 H, q, OCH2CH3), 2.88 (6 H, s, 3-CH3 and
4-CH3) and 1.37 (3 H, t, OCH2CH3); m/z (%) 324(50,M+) 278(100), 250(31), 220(10), 139(8), 125(7) and 111(8) f) Benzyl 8-fluoro-3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylate
(0.155 g, 40%) was obtained from 5-fluoroindole and the 5- acetoxymethyl-4-acetylpyrrole, it had m.p. 217-219ºC (Found: C,
74.6; H, 4.95; N, 7.3. C24H19FN2O2 require8 C, 74-6; H, 4.96; N,
7.25); δH[2H-]-DMSO) 11.36 (1 H, s, 1-NH), 10.86 (1 H, s, 5-NH),
7.94 (1 H, dd, J9 and 2.5, 9-H), 7.89 (1 H, s, 10-H), 7.56-7.38 (5 H, m, ArH), 7.39 (1 H, dd, J9 and 4, 6-H), 7.21 (1 H, dt, J9 and 2.5, 7-H), 5.42 (2 H, s, CH Ph), 2.91 and 2.90 (2 × 3H, 2 × S, 3-CH3 and 4-CH3); m/z (%) 386(68,M+), 278(100), 249(22) and 91(43). g) Ethyl 3,4,6-trimethylpyrrolo[3,2-b]carbazoIe-2-carboxylate
(0.206 g, 64.4%) was obtained from the reaction of 7-methylindole and the 5-acetoxymethyl-4-acetylpyrrole, it had m.p. 230ºC
(decomp.) (Found: C, 74.9; H, 6.25; N, 8.65. C20H20N2O2 requires
C, 75.0; H, 6.29; N, 8.74%); δH([2H-]-DMSO) 11.20 (1 H, 8,1-NH),
10.11 (1 H, s, 5-NH), 7.89(1 H, d, J7.5, 9-H), 7.84 (1 H, S, 10-H), 7.18 (1 H, d, J7.5, 7-H), 7.01 (1 H, t, J7.5, 8-H), 4.37 (2 H, q, OCH2CH3), 2.98 (3 H, s, 4-CH3), 2.91 (3 H, s, 3-CH3),
2.58 (3 H, s, 6-CH3) and 1.34 (3 H, t, OCH2CH3); m/z (%)
320(54,M+), 274(100), 246(30), 230(5), 137(9), 123(7) and
109(6). h) Benzyl 3,4,6-trimethylpyrrolo[3,2-b]carbazole-2-carboxylate
(0.167 g. 43.7%) was obtained from the reaction of 7-methylindole and the 5-acetoxymethyl-4-acetylpyrrole, it had m.p. 222ºC
(decomp.) (Found: C, 78.5; H, 5.9; N.7.25. C25H22M2O2 requires
C, 78.5; H, 5.80; N, 7.33%); δH([2H-]-DMSO) 11.27 (1 H, s,
1-NH), 10.11 (1 H, s, 5-NH), 7.89 (1 H, d, J7, 9-H), 7.85 (1 H, s, 10-H), 7.56-7.35 (5 H, m, ArH), 7.18 (1H, d, J7, 7-H), 7.08
(1H, t, J, 8-H), 5.43 (2H, S, CH2Ph), 2.99 (3H, s, 4-CH3), 2.93
(3 H, s, 3-CH3) and 2.59 (3 H, s, 6-CH3); m/z (%) 382(71,M+)
274(100), 246(19) and 91(22). i) Benzyl 3-(2-methoxycarbonylethyl)-4-methylpyrrolo[3.2-b]carbazole-2-carboxylate
(0.230 g, 52.3%) was obtained from indole and the 5-acetoxymethyl-4-acetylpyrrole, it had m.p. 211-213ºC (Found: C, 73.7;
H, 5.6; N, 6.2. C27H24N2O4 requires C, 73.6; H, 5.49; N, 6.36%); δH([2H6]-DMSO) 11.51 (1 H, s, 1-NH), 10.71 (1 H, S, 5-NH), 8.75
(1 H, d, J7.5, 9-H). 7.92 (1 H, s, 10-H), 7.57-7.44 (7 H, m, ArH, 6-H and 7-H), 7.18 (1 H, t, J7.5, 8-H), 5.43 (2 H, s, CH3Ph), 3.63 (3 H,s,OCH3), 3.59 (2 H, partially obscured, t, CH2CH3CO), 2.88 (3 H, s, 4-CH3) and 2.65 (2 H, t, CH2CO); m/z(%) 440(100,M+), 332(20), 290(47), and 91(57). j) Ethyl 3,4,5-trimethylρyrrolo[3,2-b]carbazole-2-carboxylate
(0.140 g, 16%) was obtained from the reaction (2.65 mmol scale) between N-methylindole and the 5-acetoxymethyl-4-acetylpyrrole, it had m.p. 208ºC (decomp.) (Found: C, 75.12; H, 6.40; N, 8.69,
C20H20N2O2 requires C, 74.98; H, 6.29; N, 8.74%); δH([2H6]-DMSO)
11.28 (1H, s, 1-NH), 8.08 (1H d, J7.9, 9-H), 7.88 (1H, 8, 10-H),
7.44 (2 H, m, 6-H, 7-H), 7.07-7.17 (1 H, m, 8-H), 4.36 (2 H, q, CH2CH3), 4.01 (3H, s, 5-CH3), 3.13 (3H, s, 4-CH3), 2.90 (3H, 8,
3-CH3), and 1.38 (3H, t, CH2CH3); m/z (%) 320(72,M+), 274(100),
245(16), 149(28) and 137(12); ʋmax(KBr Disc)/cm-1 3329 and 1670. k) Benzyl 3,4,5-trimethylPyrrolo[3.2-b]carbazole-2-carboxylate
(0.220 g, 57%) was obtained from the reaction between N-methyl indole and the 5-acetoxymethyl-4-acetylpyrrole in toluene at
55ºC, catalysed by toluene-4-sulphonic acid, it had m.p. 228-229°C (Found: C, 77.17 ; H, 5.73 ; N, 7.09. C25H22N2O2 · 0.33 H2O requires C, 77.31; H, 5.88 ; N, 7.21% ) ; δH( [ 2H6 ] -DMSO) 11.28 ( 1H, 8, 1-NH) , 8.03 ( 1H, d, J7.5 , 9-H) , 7.88 ( 1H, s , 10-H) , 7.56-7.34 ( 7H, m, ArH, 6-H, 7-H) , 7.15-7.07 ( 1H, m, 8-H) , 5.40 (2H, s, CH2Ph) , 4.02 ( 3H, s , 5-CH3) , 3.14 ( 3H, s, 4-CH3 ) and 2.91
(3H, s, 3-CH3); m/z (%) 382(72,M+), 291(4), 274(100) and 91(34); ʋmax(KBr Disc)/cm-1 3337 and 1674. l) Ethyl 1,3,4-trimethylpyrrolo[3.2-b]carbazole-2-carboxylate
(0.060 g, 7%) was obtained from the reaction (2.5 mmol scale) between indole and the 5-acetoxymethyl-4-acetylpyrrole, it had m.p. 188-189°C (Found: C, 74.86; H, 6.32; N, 8.65), C20H20N2O2 requires C, 75.98; H, 6.29; N, 8.74%); δH([2H6]-DMSO) 10.66 (1
H, s, 5-NH), 8.14 (1 H, d, J7.7, 9-H), 8.03 (1H, s, 10-H),
7.45-7.31 (2H, m, 6-H, 7-H), 7.06-7.15 (1 H, m, 8-H), 4.38 (2 H, q, CH2CH3), 3.98 (3H, s, 1-CH3), 2.91 (3H, s, 4-CH3), 2.83 (3H, s, 3-CH3) and 1.38 (3H, t, CH2CH3); m/z (%) 320(M+,100),
306(10), 292(30), 247(8) and 231(10); ʋmax(KBr Disc)/cm-1 3385 and 1657. m) Benzyl 1,3,4-trimethylpyrrolo[3,2-b]carbazole-2-carboxylate
(0.240 g, 28%) was obtained from the reaction (2.7 mmol scale) between indole and the 5-acetoxymethyl-4-acetylpyrrole, it had m.p. 186-187°C (Found: C, 78.63; H, 5.83; N, 7.32, C25H22N2O2 requires C, 78.51; H, 5.80; N, 7.32%); δH([2H6]-DMSO) 10.66 (1H, s, 5-NH), 8.14 (1 H, d, J7.4, 9-H), 8.02 (1 H, S, 10-H),
7.56-7.31 (7H, m, ArH, 6-H, 7-H), 7.06-7.15 (1 H, m, 8-H), 5.41
(2H, s, CH2Ph), 3.98 (3H, s, 1-CH3), 2.90 (3H, s, 4-CH3) and
2.83 (3H, s, 3-CH3); m/z (%) 382 (M ,100), 338(10), 291.44).
247(18) and 231(10); ʋmax(KBr Disc) /cm-1 3443 and 1697. n) Ethyl 1,3,4,5-tetramethylpyrrolo[3,2-b]carbazole-2-carboxylate
(0.220 g, 30%) was obtained from the reaction (2.2 mmol scale) between N-methylindole and the 5-acetoxymethyl-4-acetylpyrrole, it had m.p. 165.5-167ºC (decomp.) (Found: C, 75.50: H, 6.65; N,
8.30, C21H22N2O2 requires C, 75.47; H, 6.63; N, 8.38%);
δH([ 2H6 ]-DMSO 8.15 (1 H, d, J7.5, 9-H), 8.07 (1H, s, 10-H),
7.50-7.38 (2H, m, 6-H, 7-H), 7.09-7.19 (1 H, m, 8-H), 4.38 (2 H, q, CH2CH3), 4.03 (3H, s, 5-CH3), 3.96 (3H, s, 1-CH3), 3.14 (3H, s, 4-CH3), 2.84 (3H, s, 3-CH3) and 1.39 (3H, t, CH2CH3); m/z (%)
334 ( 100,M+), 306(18) and 245(6); ʋmax(KBr Disc)/cm-11690 and
1528. o) Benzyl 1,3,4,5-tetramethylpyrrolo[3.2-b]carbazole-2-carboxylate
(0.070 g, 13%) was obtained from the reaction (1.4 mmol scale) between N-methylindole and the 5-acetoxymethyl-4-acetylpyrrole, it had m.p. 196-198°C (Found: C, 78.45; H, 6.16; N, 6.94, C26H24N2O2 requires C, 78.76; H, 6.10; N, 7.07%); δH([ 2H6 ]-DMSO)
8.15 (1 H d, J7.8, 9-H), 8.07 (1 H, s, 10-H), 7.59-7.29 (7H, m, ArH, 6-H, 7-H), 7.10-7.20 (1 H, m, 8-H), 5.42 (2H, s, CH2Ph) ,
4.03 (3H, s, 5-CH3), 3.97 (3H, s, 1-CH3), 3.13 (3H, 8, 4-CH3) and 2.83(3H, s, 3-CH3); m/z (%) 396(100,M+), 305(38), 245(16) and 235(10); ʋmax KBr Disc)/cm-1 1696 and 1529. p) Benzyl 3,4-dimethyl-5-(4-toluenesulphonyl.pyrrolo[3,2-b]carbazole-2-carboxylate
(0.012 g, 4%) was obtained from the reaction (0.6 mmol scale) between N-(4- toluenesulphonyl)indole and the 5-acetoxymethyl- 4-acetylpyrrole, it had m.p. 270ºC (Found: C, 70.83; H, 5.01; N,
5.23, C31H26N2O4S requires C, 71.24; H, 5.01; N, 5.36%); δ H([2H6]-DMSO) 11.58 (1H, s, N-H), 8.28-8.08 (3H, m, 6-H, 9-H,
10-H), 7.66-7.21 (11H, m, ArH, TsH, 7-H, 8-H), 5.40 (2H, s, CH2Ph), 3.04 (3H, 8, 4-CH3), 2.88 (3H, s, 3-CH3) and 2.20 (3H, s, Ts-CH ); m/z (%) 523(30, (M+l), 446(20), 367(30), 348(56),
33(100), 295(30) and 274(90); ʋmax(KBr Disc) /cm-1 3558 and 1666.
q) Ethyl 7-acetoxy-3.4-dimethyl-6-methoxypyrrolo[3,2-b]carbazole -2-carboxylate
(7%) obtained from the reaction between 6-acetoxy-7-methoxyindole and the 5-acetoxymethyl-4-acetylpyrrole, it had m.p.
241-244°C. δH (CDCl.) 8.59 (1H, s, br, NH), 7.78 (1H, s, br,
NH), 7.76 (1H, 8, 10-H), 7.74 (1H, d, J8, 9-H), 6.88 (1H, d, J8, 8-H), 4.44 (2H, q, CH2CH3), 4.04 (3H, s, 6-OCH3), 2.96 and 2.92 (2 × 3H, 2 × s, 4-CH3 and 3-CH3), 2.42 (3H, s, 7-CH3COO) and
1.46 (3H, t, CH2CH3); m/z (%) 394 (100, M+), 352 (47), 348 (33), 306 (87), 263 (21) and 87 (73); ʋmax(Nujol)/cm-1 3413, 3341,
1739 and 1675; ʋmax (MeOH)/nm 405, 386, 339, 325, 305, 269, 240 and 226. (Found: M+, 394, 1529. C H N O requires 394.1529). r) Ethyl 9-methoxy-3,4,5-trimethylpyrrolo[3,2-b]carbazole-2-carboxylate obtained from the reaction between 4-methoxy-l-methylindole and the 5-acetoxymethyl-4-acetylpyrrole, it had m.p. 263-266 C
(Found: C, 71.84; H, 6.34; N, 7.91. C21H22N2O3 requires C,
71.98; H, 6.33; N, 7.99%; δH (CDCl3) 8.60 (1H, s, br, NH), 8.15
(1H, s, 10-H), 7.40 (1H, t, J8, 7-H), 6.95 (1H, d, J8, 6-H), 6.66 (1H, d, J8, 8-H), 4.43 (2H, q, OCH CH ), 4.10 and 4.04 (2 × 3H, 2 × s, N-CH and OCH3), 3.19 (3H, s, 4-CH3), 2.98 (3H, s, 3-CH3) and 1.46 (3H, t, OCH2CH3); m/z (%) 350 (74, M+), 304
(100), 276 (17), 223 (10) and 152 (19).
s) Benzyl 8-chloro-3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylate
(0.069 g; 17%) was obtained from the reaction between
5-chloroindole and the 5-acetoxymethyl-4-acetylpyrrole, it had m.p. 215-220ºC. (decomp.). (Found: C, 71.42; H, 4.96; N, 7.11,
C24H1 9ClN2O2 requires C, 71.55; H, 4.75; N, 6.95%); δH
(2[H]6-DMSO) 11.39 (1H, B, 1-NH), 10.84 (1H, s, 5-NH), 8.17 (1H, s, 9H), 7.93 (1H, s, 10-H), 7.54 (1H, d, J7, 7-H), 7.48-7.34
(6H, m, ArH and 6-H), 5.42 (2H, s, CH2Ph) and 2.88 (6H, s, 3-CH3 and 4-CH3); m/z (%) 402 (30, m+), 358(5), 294 (65), 267 (25) and
91 (100). The crystallisation liquors were submitted to flash chromatography on silica. Elution with ethyl acetate/light petroleum yielded further title compound which was crystallised from ethyl acetate (0.030g; 7%) and 3- (3 '-acetyl-5'-benzyloxycarbonyl-4'-methylpyrrol-2'-ylmethyl)-5-chloroindole as cream coloured crystals (0.152g; 36%) m.p. 141-143ºC (Found: C, 68.20;
H, 5.18; N, 6.60; C24H21ClN2O3 requires C, 68.49; H, 5.03; N,
6.65); δH (CDCl3) 8.72 (1H, s, 1'-NH), 8.26 (1H, s, 1-NH), 7.38
(1H, d, J2, 4-H), 7.35 (6H, m, ArH and 6-H), 7.18 (1H, dd, J8 and 2, 2-H), 7.09 (1H, d, J2, 2-H), 5.23 (2H, s, CH Ph), 4.39 (2H, s, 3-CH3), 2.64 (3H, s, 4'-CH3) and 2.52 (3H, s, CH3CO); m/z (%) 420 (20, M+), 405 (10), 311 (20), 151 (15) and 91 (100). t) Ethyl 3,4-dimethyl-8-hydroxypyrrolo[3.2-b]carbazole-2-carboxylate
Obtained from the reaction between 5-hydroxyindole and the
5-acetoxymethyl-4-acetylpyrrole and crystallised from methanol, it had m.p. 250°C (decomp.) δH[ 2H6 ]-DMSO) 11.11 (1H, s, 1-NH),
H o
10.21 (1H, s, 5-NH), 8.83 (1H, s, OH), 7.73 (1H, s, 10-H), 7.37
(1H, d, J2.5, 9-H), 7.21 (1H, d, J8, 6-H), 6.87 (1H, dd, J8 and
2.5, 9-H), 7.21 (2H, q, OCH2CH3), 2.87 (3H, 8, 4-CH3), 2.84 (3H, s, 3-CH3) and 1.38 (3H, t, OCH2CH3) ; m/z (%) 322 (69, M+), 276
(100), 248 (24), 220(3) and 138 (15); (Found: M+, 322. 1322 C19H18N2.O3 requires M, 322. 1317). Also isolated from the reaction was 3-(3'-acetyl-5'-ethoxycarbonyl-4'-methylpyrrol-2'- ylmethyl)-5-hydroxyindole, it had m.p. 99-102 C (Found: C, 66.89:
H, 6.17; N, 8.03. C19H20N2O4 requires C, 67.04; H, 5.92; N,
8.23%) δH(CDCl3) 8.84 (1H, S, 1'-NH), 8.14 (1H, s, 1-NH), 7.20
(1H, d, J8, 7-H), 7.10 (1H, d, J 2.5, 2-H), 6.81 (1H, d, J1.5,
4-H), 6.79 (1H, dd, J1.5 and 8, 6-H), 5.60 (1H, s, br, 5-OH), 4.31 (2H, s, CH2), 4.21 (2H, q, OCH2CH3 , 2.58 (3H, s, 4'-CH3) 2.48 (3H, s, 3'-COCH3) and 1.27 (3H, t, OCH3CH3); m/z (%) 340 (100, M+), 325 (44), 293 (21), 279 (35), 266 (35), 251 (31), 223 (25), 196 (5), 147 (20) and 133 (36). u) Benzyl 3 ,4-dimethyl-7-fluoropyrrolo[3,2-b]carbazole-2-carboxylate and Benzyl 3,4-dimethyl-8-fluoro-Pyrrolo[2,3-b]carbazole-2- carboxylate obtained as a mixture of isomers from the reaction between
6-fluoroindole and the 5-acetoxymethyl-4-acetylpyrrole.
Chromatographic separation yielded the [3.2-b]isomer (0.139g,
36%) m.p. 205ºC (decomp.) δH([ 2H6 ]-DMSO) 11.32 (1H, s, 1-NH),
10.85 (1H, 8, 5-NH), 8.08 (1H, dd, J9 and 6, 9-H), 7.86 (1H, s, 10-H), 7.57-7.35 (5H, m, ArH), 7.12 (1H, dd, J10 and 2, 6-H), 6.90 (1H, dt, J9 and 2, 8-H), 5.43 (2H, s, CH Ph), 2.91 (3H, s, 3-CH3) and 2.90 (3H, s, 4-CH3); m/z (%) 386 (55, M+), 342(5), 295 (4), 278(100), 249(45), 236(20), 222 (25) and 91 (95); (Found: MH+, 387. 1509. C24H20FN2O2 requires 387.1509); and the
[2,3-b]isomer m.p. 190-193°C δH(CDCl3) 8.54 (1H, s, br, 1-NH),
8.10 (1H, dd, J9 and 6, 5-H), 7.87 (1H, s, br, 9-NH), 7.51-7.34 (5H, m, ArH), 7.34 (1H, s, 10-H), 7.22 (1H, dd, concealed by 10-H, 8-H), 6.93 (1H, dt, J2 and 9, 6-H), 5.41 (2H, s, CH2Ph),
3.20 (3H, s, 4-CH ) and 3.00 (3H, s, 3-CH3); m/z (%) 386 (100, M+), 295 (12), 278 (96), 250 (27), 236 (7), 222(8) and 91 (59);
(Found: M+, 386.1433. C24H19FN2O2 reqμires 386.1431). v) Ethyl 3,4-dimethyl-7-fluoropyrrolo[3.2-b]carbazole-2-carboxylate and Ethyl 3,4-dimethyl-8-fluoropyrrole[2.3-b]carbazole-2-carboxylate obtained as a mixture of isomers from the reaction between 6-fluoroindole and the 5-acetoxymethyl-4-acetylpyrrole. Chromatographic separation yielded the [3.2-b]isomer which was
crystallised from dichloromethane, m.p. 231-234 C (Found: C,
70.45; H, 5.53; N, 8.66. C19H17FN2O2 requiree C, 70.36, H,
5.28; N, 8.64%); δH([ 2H6 ]-DMSO) 11.27 (1H, s, br, 1-NH), 10.82
(1H, a, br, 5-NH), 8.90 (1H, dd, J9 and 6, 9-H), 7.85 (1H, s, 10-H), 7.12. (1H, dd, J10 and 2, 6-H) 6.89 (1H, dt, J2 and 9,
8-H), 4.37 (2H, q, OCH2CH3 ) 2-89 (6H, s, 4-CH3), 1.39 (3H, t, OCH2CH3); m/z (%) 324 (60, M+), 278 (100), 250 (34), 222 (10) and 139 (7); (Found; M+, 324.1267. C19H17FN2O2 requires
324.1274); and the [2,3-b]isomer m.p. 262-265°C; δH( [2H-)-DMSO)
11.14 (1H, s, br, 1-NH), 11.06 (1H, s, br, 9-NH), 8.12 (1H, dd J 6 and 9, 5-H), 7.19 (1H, s, 10-H), 7.15 (1H, dd J10 and 2, 8-H), 6.92 (1H, dt, J2 and 9, 6-H), 4.36 (2H, q, OCH2CH3), 3.13 (3H, s, 4-CH3), 2.93 (3H, s, 3-CH3) and 1.39 (3H, t, OCH2CH3); m/z (%) 324 (72, M+), 278 (100), 250 (39), 222 (9), 139(6) and 125(7); (Found: M+, 324.1280. C19H17FN2O2 requires 324.1274). w) Ethyl 3,4-dimethyl-9-hydroxypyrrolo[3,2-b]carbazole-2-carboxylate and Ethyl 3,4-dimethyl-5-hydroxypyrrolo[3,2-b]carbazole-2- carboxylate obtained as a mixture of isomers from the reaction between 4-hydroxyindole and the 5-acetoxymethyl-4-acetylpyrrole.
Chromatographic separation yielded the [ 3,2-b]isomer which was crystallised from ethyl acetate/light petroleum, m.p. 260-262ºC (decomp.) δd([ 2H6 ]-DMSO) 11.13 (1H, s, 1-NH), 10.56 (1H, s,
5-NH), 10.00 (1H, s, OH), 8.02 (1H, 8, 10-H), 7.12 (1H, t, J7.5, 7-H), 6.83 (1H, d, J7.5, 6-H), 6.48 (1H, d, J7.5, 8-H), 4.39 (2H, q, OCH2CH3), 2.87 (3H, 8, 4-CH3), 2.85 (3H, s, 3-CH3) and 1.38 (3H, t, OCH2CH3); m/z (%) 322 (61, M+), 276 (100), 248 (20), 219 (5) and 138 (11); (Found: M+, 322.1305. C19H18N2O3 requires 322.1317); and the [2,3-b]isomer which was crystallised from ethyl acetate, m.p. 251-254ºC (decomp.) δH([ 2H6 ]-DMSO)
10.95 (1H, s, 1-NH), 10.85 (1H, s, 9-H), 9.89 (1H, s, OH), 7.08 (1H, t, J7.5, 7-H), 7.07 (1H, s, 10-H), 6.77 (1H, d, J7.5, 8-H), 6.52 (1H, d, J7.5, 7-H), 4.32 (2H, q, OCH2CH3), 3.44 (3H, s,
4-CH3), 2.92 (3H, s, 3-CH3) and 1.37 (3H, t, OCH2CH3); m/z (%)
322 (65, M+), 276 (100), 248 (88), 219 (15), 205(10), 191 (10),
178 (5), 165 (5), 138 (10) and 115 (10); (Found:M+, 322.1317.
C19H18N2O3 requires 322.1317). x) Ethyl 6,99-dimethoxy-3,4-dimethylpyrrole[3,2-b]carbazole-2- carboxylate and Ethyl 5,8-dimethoxy-3,4-dimethylpyrrolo[2.3-b] carbazole-2-carboxylate obtained as a mixture of isomers from the reaction between
4,7-dimethoxyindole and the 5-acetoxymethyl-4-acetylpyrrole.
Chromatographic separation yielded the [3.2-b]isomer (13.7%) m.p. 256-258°C. (Found: C, 68.98; H, 6.23; N, 7.89. C21H22N2O4 requires C, 68.84; H, 6.05; N, 7.65%). δH (CDCl3) 8.58 (1H, s, br, NH), 8.08 (1H, s, 10-H), 7.84 (1H, s, br, NH), 6.82 (1H, d, J8, 7-H), 6.50 (1H, d, J8, 8-H), 4.43 (2H, q, OCH2CH3), 4.05
(3H, s, 9-OCH3), 3.98 (3H, s, 6-OCH3), 2.96 (3H, 8, 4-CH3), 2.92 (3H, s, 3-CH3) and 1.44 (3H, t, OCH2CH3); m/z (%) 366 (73, M+),
326 (100), 305 (11), 290 (11), 277 (23), 262 (15), 183(10), 160
(17), 152 (19) and 131(7); ʋmax (Nujol)/cm-1 3474, 3323 and
1674; λ (MeOH)/nm 415, 387, 344, 330(sh), 305(sh), 266, 246 and 220; and the [2,3-b]isomer (9.3%) m.p. 193-195°C. (Found: C,
69.03; H, 6.29; N, 7.42. C2ιH22N2O4 requires C , 68.84, H, 6.05;
N, 7.65%); δH (CDCl3) 8.44 (1H, s, br, NH), 8.10 (1H, s, br,
NH), 7.06 (1H, s, 10-H), 6.82 (1H, d, J8, 7-H), 6.56 (1H, d, J8, 6-H), 4.40 (2H, q, OCH2CH3), 3.98 (3H, 8, OCH3), 3.97 (3H, 8, OCH3), 3.42 (3H, s, 4-CH3), 3.00 (3H, s, 3-CH3) and 1.43 (3H, t, OCH2CH3 ); m/z (%) 366 (100, M+), 320 (82), 292 (20), 277 (24), 262 (10), 183 (14), 160 (28), 131 (3); ʋmax (Nujol)/cm-1 3457,
3345 and 1660; ʋmax (MeOH)/nm 381, 365, 293, 247 and 219. y) Ethyl 7-methoxy-3.4-dimethylpyrrole[3,2,b1carbazole-2-carboxylate and Ethyl 7-methoxy-3.4-dimethylpyrrole[2,3-b]carbazole-2- carboxylate
obtained as a mixture of isomers from the reaction between 6-methoxyindole and the 5-acetoxymethyl-4-acetylpyrrole. Flash chromatography on silica, eluting with ethyl acetate/cyclohexane
(1:1) yielded the [3,2-blisomer which was crystallised from ethylacetate/cyclohexane, m.p. 239-241ºC (decomp.) δH([2H6]- DMSO) 11.09 (1H, s, 1-NH), 10.49 (1H, s, 5-NH), 7.91 (1H, d, J
8.7, 9-H), 7.73 (1H, s, 10-H), 6.88 (1H, d, J2.3, 6-H), 6.68
(1H, dd, J 8.7 and 2.3, 8-H), 4.35 (2H, q, OCH2CH3), 3.84 (3H, s, 7-OCH3), 2.87 (3H, s, 3-CH3), 2.86 (3H, s, 4-CH3) and 1.37
(3H, t, OCH2CH3); m/z (%) 336 (84, M+), 290 (100), 262 (32), 247
(16) and 219 (16); ʋmax (KBr Disc)/cm-13342, 1674 and 1628; and the [2,3-blisomer which was crystallised from ethyl acetate/cyclohexane, m.p. 260 C (decomp.) δH([2H6]-DMSO) 10.98
H o
(1H, s, 1-NH), 10.74 (1H, s, 9-NH), 8.00 (1H, d, J8.7, 5-H), 7.13 (1H, s, 10-H), 6.87 (1H, d, J2.7, 8-H), 6.70 (1H, dd, J8.7 and 2.7, 6-H), 4.34 (2H, q, OCH2CH3), 3.83 (3H, s, 7-OCH3), 3.10 (3H, s, 4-CH3), 2.91 (3H, 8, 3-CH3) and 1.37 (3H, t, OCH2CH3); m/z (%) 336 (56, M+), 290 (70), 262 (26), 145 (16), 129 (14); ʋmax (KBr Disc) /cm-13379, 3339 and 1663. z) Ethyl 3-ethyl-4-methylpyrrolo[3.2-b]carbazole-2-carboxylate
(0.956g, 27%) was obtained from the reaction (11 mmol scale) between indole and the ethyl 5-acetoxymethyl-4-acetyl-3-ethylpyrrole-2-carboxylate, after recrystallisation from toluene, it had m.p. 248-249ºC (decomp.) (Found: C, 74.93; H, 6.35; N, 8.60.
C20H20N2O2 requires: c, 74.98; H, 6.29; N, 8.74%); δH([2H6]- DMSO) 11.27 (1H, S, 1-NH), 10.63 (1H, s, 5-NH), 8.09 (1H, d, J 8, 9-H), 7.93 (1H, 8, 10-H), 7.31-7.47 (2H, m, 6-H, 7-H), 7.09 (1H, ddd, J 8, 5.5, 2, 8-H), 4.40 (2H, q, J 7, CO2CH2), 3.37 (2H, q, J 7, 3-CH2), 2.91 (3H, S, 4-CH3), 1.41 (3H, t, J 7, CO2CH2CH3), 1.30 (3H, t, J 7.5, 3-CH2CH3); m/z (%) 320(100, M+), 274(96);
ʋmax (KBr disc) /cm-1 3344, 3327, 1680, 1664, 1238.
Example 5
Pyrrolo[3.2-b]carbazole-2-carboxylic Acids General procedure.
To a solution of the benzyl pyrrolo[3,2-b}carbazole-2-carboxylate in dry tetrahydrofuran (THF) (10 cm3) was added 10% Pd-on-C (50 mg). The reaction mixture was hydrogenated at one atmos. pressure and room temperature. After uptake of H2 had ceased, the catalyst was removed by filtration through Celite and washed well with THF, and the combined filtrates were evaporated under reduced pressure.
Crystallisation of the resulting solid from acetone, methyl ethyl ketone or aqueous methanol gave the pyrrolo[3,2-b]carbazole-2-carboxylic acids as yellow crystals. a) 3,4-Dimethylpyrrolo[3,2-b]carbazole-2-carboxylic acid
(0.234 g, 84.3%) m.p. 237°C (decomp.); δH([2H6]-DMSO) 12.74 (1
H, br, s, CO H), 11.13 (1 H, s, 1-NH), 10.60 (1 H, s, 5-NH),
8.05 (1 H, d, J7.5, 9-H), 7.87 (1 H, s, 10-H), 7.42 (1 H, d,
J7.5, 6-H), 7.36 (1 H, t, J7.5, 7-H), 7.08 (1 H, t, J7.5, 8-H),
2.92 and 2.91 (2 × 3H, 2 × S, 3-CH and 4-CH3); m/z(%)
278(30,M+) 260(39), 234(100), 218(19), 204(8), 167(8) 149(16),
130(10) and 117(25) (Found: M+, 278.1060.C17H14N2O2 requires
M,278.1055). b) 8-Fluoro-3,4-dimethylpyrrolo[3,2-b]2arbazole-2-carboxylic acid
(0.0845 g, 85.6%) m.p. 236-239 °C, δH([2H6]-DMSO) 12.80 (1 H, br, s, CO H), 11.19 (1 H, s, 1-NH), 10.60 (1 H, s, 5-NH), 7.91 (1 H, dd, J9 and 2.5, 9-H), 7.86 (1 H, s, 10-H), 7.37 (1 H, dd, J9 and 4, 6-H), 7.20 (1 H, dt, J9 and 2.5, 7-H) and 2.89 (6 H, s, 2×CH3); m/z (%) 296(51,M+), 278(71), 252(100), 250(37), 236(19), 222(13), 139(22), 12b(36) and 111(28) (Found: M+, 296.0960. C17H13FN2O2 requires M, 296.0961)
c) 3,4,6-Trimethylpyrrolo[3,2-b]carbazole-2-carboxylic acid
(0.065 g, 85%) m.p. 230 C (decomp.) (Found: C,74.2; H,5.55; N,9.4 C18H16N2O2 requires C, 74.0; H, 5.52; N, 9.58%);
δH([2H6]-DMSO) 12.80 (1 H, br, s, CO2H), 11.01 (1 H, s, 1-NH),
10.08 (1 H, s, 5-NH), 7.90 (1 H, d, J7.5, 9-H), 7.82 (1 H, 8, 10-H), 7.16 (1 H, d, J7.5, 7-H), 7.01 (1 H, t, J7.5, 8-H), 2.97
(3 H, s, 4-CH3), 2.92 (3H, s, 3-CH3) and 2.58 (3 H, s, 6-CH3); m/z(%) 292(72, M+), 274(100), 246(50), 230(11),137(25), 122(24) and 109(30). d) 3-(2-Methoxycarbonylethyl)-4-methylpyrrolo[3.2-b]carbazole-2-carboxylic acid (0.0673 g, 84.6%) m.p. 255ºC (decomp.) (Found: C, 68.4; H,5.3;
N,7.75.C20H18N2O4 requires C, 68.6; H,5.18; N, 8.00%);
δH([2H6]-DMSO) 12.88 (1 H, br, s, CO2H), 11.34 (1 H,s,1-NH),
10.65 (1 H, s, 5-NH), 8.06 (1 H, d, J 7.5, 9-H), 7.88 (1 H, s, 10-H), 7.42 (1 H, d, J 7.5, 6-H), 7.36 (1 H, t, J7.5, 7-H) 7.07 (1 H, t, J7.5, 8-H), 3.66 (3 H, s, OCH3), 3.63 (2 H, partially obscured t,CH2CH2CO), 2.89 (3 H, s, 4-CH3), 2.66 (2 H, t, CH3CO); m/z(%) 350(100,M+), 332(17), 306(30), 290(63), 272(22), 259(32) and 233(47). e) 1,3,4-Trimethylpyrrolo[3,2,b]carbazole-2-carboxylic acid
(0.060 g, 44%) m.p. 215-216°C (decomp.) (Found: C, 73.69; H, 5.51; N, 9.41; C18H16N2O2 requires c, 73.95; H, 5.52; N, 9.58); δH([2H6]-DMSO) 12.94 (1H, br, 8, COOH), 10.63 (1H, s, 5-NH),
8.13 (1H, d, J 7.9, 9-H), 8.00 (1H, s, 10-H), 7.45-7.30 (2H, m,
6-H, 7-H), 7.14-7.04 (1H, m, 8-H), 3.99 (3H, s, 1-CH3), 2.91
(3H, s, 4-CH3) and 2.85 (3H, s, 3-CH3); m/z (%) 292(95,M+),
275(10), 247(40), 232(30), 180(100) and 135(100); ʋmax(KBr
Disc)/cm-13375, 2930 and 1709.
f) 3.4.5-Trimethylpyrrolo[3.2-b]carbazole-2-carboxylic acid
(0.015 g, 18%) m.p. 239-240°C (decomp.) (Found: C, 74.11; H,
5.38; N, 9.39; C18H16N2O2 requires C, 73.95; H, 5.52; N, 9.58; δH([2H6]-DMSO) 11.15 (1H, 8, 1-NH), 8.04 (1H, d, J 7.5, 9-H)
7.88 (1H, s, 10-H), 7.48-7.41 (2H, m, 6-H, 7-H), 7.17-7.06 (1H, m, 8-H), 4.03 (3H, s, 5-CH3), 3.16 (3H, s, 4-CH ) and 2.93 (3H, s, 3-CH3); m/z (%) 292(90,M+), 274(75), 232(70), 197(35),
181(60), 149(30) and 130(100); ʋmax(KBr Disc)/cm 3454, 2926 and 1670. g) 1,3,4,5-Tetramethylpyrrolo[3,2-b]carbazole-2-carboxylic acid
(0.030 g, 32%) m.p. 215-217°C (decomp.) (Found: C, 74.44; H,
6.00; N, 9.14; C19H18N2O2 requires C, 74.49; H, 5.92; N, 9.14); δH([2H6)-DMSO) 12.98 (1H, br, s, COOH), 8.14 (1H, d, J7.6, 9-H),
8.04 (1H, s, 10-H), 7.48-7.38 (2H, m, 6-H, 7-H), 7.18-708 (1H, m, 8-H), 4.01 (3H, 8, 5-CH3), 3.97 (3H, s, 1-CH3), 3.12 (3H, s,
4-CH ) and 2.84 (3H, s, 3-CH3); m/z (%) 306(100,M+), 279(25), 232(38), 197(34), 181(80) and 149(25); ʋmax(KBr Disc)/cm 1935 and 1659. h) 3,4-Dimethylpyrrolo[3.2-b]carbazole-2-carboxylic acid The ethyl ester (500mg, 1.6mmol) in water (15 cm3 ) and methanol
(35 cm3) was heated to reflux and sufficient methanol to achieve dissolution was added. Caesium carbonate (5.32g; 16mmol) was added and the mixture was heated to reflux under nitrogen for
18h. After cooling, solvent was removed in vacuo to leave approximately 20 cm of solution which was brought to pH3 by the addition of 0.1M hydrochloric acid whereupon the title compound precipitated out. Filtration, washing with water and drying under vacuum yielded analytically pure product (437mg; 96%) which was spectroscopically identical to that obtained in
Example 5a.
Example 6
Pyrrolo[3.2-b]carbazole-2-carbσxyclic Acid Esters - General procedure
The pyrrolo[3,2-b]carbazole-2-carboxylic acid (1.0 mmol) and N,N'-carbonyl diimidazole (1.1 mmol) were dissolved in freshly distilled tetrahydrofuran under a nitrogen atmosphere. The resulting suspension was stirred at room temperature for at least one hour, and complete conversion of the acid to the imidazolide intermediate was verified by TLC. The alcohol or phenol (1.5-2.0 mmol, i.e. an excess) was added in one portion; and the resulting mixture was heated to reflux until TLC showed complete consumption of the imidazolide intermediate. The product was obtained by column chromatography on silica, followed by recrystallisation. a) Phenyl 3.4-dimethylpyrrolo[3.2-b]carbazole-2-carboxylate was obtained from the reaction of the imidazolide intermediate with phenol. Chromatography (eluting with 10% acetone/90% petrol) followed by recrystallisation from acetone-petrol gave orange crystals (0.230 g, 65%) m.p. >230ºC (decomp.) (Found:
C,78.17; H, 5.09; N, 7.77. C23H18N2O2 requires C, 77.95; H,
5.12; N, 7.90%); δ([2H6]-DMSO) 11.55 (1H, S, 1-NH), 10.64 (1H, s, 5-NH), 8.10 (1H, d, J 7.5, 9-H), 7.94 (1H, s, 10-H), 7.30-7.58 (7H, m, PhH, 6-H, 7-H), 7.09 (1H, ddd, J 7.5, 5.5, 2,
8-H) and 2.97 and 2.95 (2 × 3H, 2 × s, 3-CH3 and 4-CH3); m/z(%)
355 (40,M+); ʋmax(KBr Disc)/cm-1 3396, 1701 and 1180. b) [(2-Dlmethylamino)ethyl] 3 ,4-dimethylpyrrolo[3.2-b]carbazole -2-carboxylate was obtained from the reaction of the imidazolide intermediate with (2-dimethylamino)ethanol. Chromatography (eluting with 10% methanol/90% DCM) gave a yellow solid (O.350 g, 99%).
Recrystallisation of a portion from DCM gave yellow crystals with m.p. 174.0-175.7ºC (decomp.) (Found: C, 70.46; H, 6.48; N,
11.76. C21H23N3O2.0.15CH Cl2 requires C, 70.29; H, 6.45; N,
11.55%); δH([2H6]-DMSO) 11.18 (1H, s, 1-NH), 10.60 (1H, s,
5-NH), 8.07 (1H, d, J 8, 9-H), 7.89 (1H, s, 10-H), 7.30-7.43
(2H, m, 6-H, 7-H), 7.09 (1H, ddd, J 8, 6, 2.5, 8-H), 4.41 (2H, t, J 6, OCH2), 2.91 (6H, 8, 3-CH3 and 4-CH3), 2.69 (2H, t, J
6.0, NCH2) and 2.27 (6H, 8, N(CH3 ) 2); m/z(%) 350(46, (M+l)+),
261(68) and 133(100); ʋmax(KBr Disc)/cm-13377, 1661 and 1238. c) [(3-Dimethylamino)phenyl] 3.4-dimethylpyrrolo[3.2-b]carbazole-2- carboxylate was obtained from the 0.95 mmol scale reaction of the imidazolide intermediate with (3-dimethylamino)phenol. Chromatography (eluting with 10% ethyl acetate/90% toluene) followed by recrystallisation from ethyl acetate gave yellow crystals (0.272 g, 72%) m.p. 240-242°C (decomp.) (Found: C, 75.37; H, 5.71; N,
10.36. C25H23N3O2 requires C, 75.55; H, 5.83; N, 10.57%; δH
([2H6]-DMSO) 11.49 (1H, s, 1-NH), 10.64 (1H, s, 5-NH), 8.08 (1H, d, J 8, 9-H), 7.91 (1H, s, 10-H), 7.34-7.48 (2H, m, 6-H, 7-H),
7.27 (1H, t, J 8, 5'-H), 7.10 (1H, ddd, J 8, 6, 2, 8-H),
6.56-6.70 (3H, m, 2'-H, 4'-H, 6'-H), 2.96 (3H, s) and 2.94
(9H,s) (3-CH3,4-CH3, N(CH3)2); m/z (%) 398(38, M+l)+, 261(25),
232(21) and 217(100); ʋmax(KBr Disc)/cm-1 3350, 1674, 1610 and
1232. d) (3-Pyridyl) 3.4-dimethylpyrrolo[3.2-b]carbazole-2-carboxylate was obtained from the reaction of the imidazolide intermediate with 3-hydroxypyridine. Chromatography (eluting with 50% ethyl acetate/50% petrol) followed by recrystallisation from acetone gave yellow crystals (0.230 g, 65%) with m.p. >270 C (decomp.)
(Found: C, 73.88; H, 4.76; N, 11.50. C22H17N3O2.0.2H2O requires: C, 73.61; H, 4.89; N, 11.71%); δH ([2H6]-DMSO) 11.59
(1H, s, 1-NH), 10.65 (1H, s, 5-NH), 8.63 (1H, d, J 2, 2'-H),
8.55 (1H, dd, J 4, 1, 6'-H), 8.10 (1H, d, J 8, 9-H), 7.90 (1H,
8, 10-H), 7.86 (1H, ddd, J 8, 3, 1, 5'-H), 7.58(1H, dd, J 8, 5,
4'-H), 7.32-7.45 (2H, m, 6-H, 7-H), 7.09(1H, ddd, J 8, 6, 2,
8-H) and 2.97 and 2.94 (2 x 3H, 2 × s, 3-CH and 4-CH3); m/z (%)
356(15, (M+l)+). ʋmax(KBr Disc)/cm-1 3377, 1715 and 1173. e) (4-Carbamoylphenyl) 3.4-dimethylpyrrolo[3.2-b]carbazole-2- carboxylate was obtained from the reaction of the imidazolide intermediate with 3-hydroxybenzamide. Recrystallisation from ethanol gave a yellow powder, and an impure residue. The latter material was further purified by column chromatography on silica (eluted with
5%) methanol/95% DCM then 10% methanol/90% DCM) followed by recrystallisation from ethanol. (0.262 g, 66%) m.p. >250ºC
(decomp.) (Found: C, 71.72; H, 4.81; N, 10.26. C24H19N3O3·0.2H2O requires C, 71.88; H, 4.88; N, 10.48%); δH ([2H6]-DMSO 11.56
(1H, s, 1-NH), 10.63 (1H, s, 5-NH), 7.90-8.12 (5H, m, 9-H, 10-H,
3'-H, 5'-H, amide N-H), 7.33-7.49 (5H, m, 6-H, 7-H, 2'-H, 6'-H, amide N-H), 7.09 (1H, ddd, J 8.5, 6, 1.5, 8-H) and 2.95 and 2.93
(2 × 3H, 2 × s, 3-CH and 4-CH3); m/z (%) 398(10, (M+l)+),
279(100); ʋmax(KBr Disc)/cm-1 3423, 1717, 1695 and 1171. f) (Pyridyl-4-methyl) 3,4-dimethylpyrrolo[3.2-b]carbazole-2- carboxylate was obtained from the reaction of the imidazolide intermediate with 4-pyridylcarbinol. Chromatography (eluting with ethyl acetate/petrol, gradient 60%, 80%, 100% ethyl acetate, then methanol/ethyl acetate, gradient 10%, 20%) followed by recrystallisation from tetrahydrofuran gave orange crystals (0.168 g, 46%) with m.p. >240ºC(decomp.) (Found: C, 72.16; H,
5.12; N, 10.73. C23H19N3O2.0.7H2O requires C, 72.31; H, 5.38; N,
11.00%); δH([2H6]-DMSO 11.31 (1H, s, 1-NH), 10.62(1H, s, 5-NH),
8.62 (2H, dd, J 4.5, 0.5, 2'-H, 6'-H), 8.08 (1H, d, J 7.5, 9-H), 7.89 (1H, s, 10-H), 7.53(2H, d, J 5.5, 3'-H, 5'-H), 7.32-7.43 (2H, m, 6-H, 7-H), 7.07 (1H, ddd, J 8, 5, 1, 8-H), 5.45 (2H, s,
ArCH2) and 2.94 and 2.92 (2 × 3H, 2 × 3-CH3 and 4-CH3); m/z (%)
369(27, (M+l)+), 327(70) and 295(100); ʋmax(KBr Disc)/cm-13400,
1709 and 1232. g) (1,3-Dibenzyloxypropyl-2) 3,4-dimethylpyrrolo[3,2-b]carbazole-2- carboxylate was obtained from the 1.5 mmol scale reaction of the imidazolide intermediate with (l,3-dibenzyloxy-2-propanol). Chromatography
(eluting with 20% ethyl acetate/80% toluene then 40% ethyl acetate/60% toluene) followed by recrystallisation from ethyl acetate-ether-petrol gave yellow crystals (0.776 g, 97%) m.p.
124.8-126°C (decomp.) (Found: C, 76.35; H, 6.07; N, 5.12.
C34H32N2O4 requires C, 76.67; H, 6.06; N, 5.26%;
δH([2H6]-DMSO) 11.18 (1H, s, 1-NH), 10.60 (1H, s, 5-NH), 8.06
(1H, d, J 7.5, 9-H), 7.88 (1H, s, 10-H), 7.22-7.42 (12H, m, 2 × PhH5, 6-H, 7-H), 7.07 (1H, ddd, J 8, 6.5,1.5, 8-H), 5.44 (1H, quintet, J 5, 1'-H), 4.60 and 4.53 (2 × 2H, 2 × dd, J 12, 2 × PhCH2O), 3.77 (4H, d, J 5.5, OCH(CH2)2) and 2.91 and 2.89 (2 ×
3H, 2 × s, 3-CH and 4-CH3); m/z(%) 532(50,M+), 260(65) and 91(100); ʋmax(KBr Disc) /cm-13358, 1681 and 1234. h) (4-Methylsulphinylphenyl) 3.4-dimethylpyrrolo[3,2-b]carbazole-2- carboxylate was obtained from the reaction of the imidazolide intermediate with 4-methylsulphinylphenol. Chromatography (eluting with ethyl acetate/petrol, gradient 90%, 95%, 98%, 100% ethyl acetate, then 10% methanol/ethyl acetate) followed by recrystallisation from tetrahydrofuran gave a yellow powder (0.261 g, 63%) m.p. >230ºC (decomp.) (Found: C, 68.40; H, 4.81;
N, 6.44. C24H20N2O3S.0.3H2O requires C, 68.32; H, 4.92; N,
6.64%; δH([2H6]-DMSO) 11.59 (1H, β, 1-NH), 10.68 (1H, 8, 5-NH),
8.10 (1H, d, J 8, 9-H), 7.93 (1H, s, 10-H), 7.82(2H, d, J 9.5,
3'-H, 5'-H), 7.59 (2H, d, J 9.5, 2'-H, 6'-H), 7.33-7.45 (2H, m,
6-H, 7-H), 7.09 (1H, ddd, J 8, 6, 2.5, 8-H), 2.99 and 2.95 (2 ×
3H, 2 × s, 3-CH3 and 4-CH3) and 2.82 (3H, s, CH3SO); m/z (%)
417(2, M+l+), 261(100) and 233(75); ʋmax (KBr Disc/cm-1 3427,
3288, 1717 and 1200. i) Methyl 3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylate was obtained from the reaction of the imidazolide intermediate with methanol. Chromatography (eluting with 30% ethyl acetate/petrol), followed by recrystallisation from ethyl acetate gave a yellow powder (0.188g, 64%) with m.p. 211-213ºC
(decomp.) (Found: C,74.06, H, 5.49, N,9.42, C18H16N2O2 requires:
C, 73.95; H, 5.52; N, 9.58%); δH([2H6]-DMSO) 11.25 (1H, s,
1-NH), 10.62 (1H, 8, 5-NH), 8.08 (1H, d, J 8, 9-H), 7.89 (1H, 8, 10-H), 7.33-7.58 (2H, m, 6-H, 7-H), 7.09 (1H, ddd, J 8, 6, 1,
8-H), 3.92 (3H, s, OCH3), 2.92 and 2.91 (2 × 3H, 2 × s, 3-CH3 and 4-CH3); m/z (%) 292(68, M+), 260(100), 232(39); ʋmax (KBr disc)/cm-1 3342, 1684 and 1236. j) [(2-Methylsulphonyl)ethyl]3.4-dimethylpyrrolo[3,2-b]carbazole-2- carboxylate
Was obtained from the reaction of the imidazolide intermediate with (2-methylsulphonyl)ethanol. Chromatography (gradient elution with ethyl acetate/petrol, 30% - 100%) followed by recrystallisation from acetone gave fine yellow crystals (0.222 g, 58%) with m.p. 255-255ºC (decomp.) (Found: C, 62.23; H, 5.25;
N, 7.08. C20H20N2O4S requirea C, 62.48; H, 5.24; N, 7.29%); δH[2H6]-DMSO 11.19 (1H, s, 1-NH), 10.60 (1H, 8, 5-NH), 8.09 (1H, d, J 7.5, 9-H), 7.89 (1H, β, 10-H), 7.32-7.45 (2H, m, 6-H, 7-H), 7.09 (1H, ddd, J 7.5, 5.5, 3, 8-H), 4.69 (2H, t, J 5.5, OCH2), 3.69 (2H, t, J 5.5, SO2CH2), 3.12 (3H, s, SO2CH2), 2.93 (6H, s,
3-CH, and 4-CH3); m/z (%) 384 (17, M+), 260(13), 59(100); ʋmax
(KBr disc)/cm-1 3387, 1661, 1234. k) Tert-butyl 3 ,4-dimethylpyrrolo[3.2-b]carbazole-2-carboxylate
The pyrrolo[3,2-b]carbazole-2-carboxylic acid (0.86 mmol) and triphenylphosphine (0.91 mmol, 1.05 eq.) were dissolved in freshly distilled tetrahydrofuran under a nitrogen atmosphere.
Tertiary-butanol (2.12 mmol, 2.5 eq.) was added by syringe, and finally diethyl azodicarboxylate (0.95 mmol, 1.1 eq.) was added dropwise over 10 minutes. The resulting suspension was stirred at room temperature for two hours, by which time TLC showed complete consumption of the starting acid. The title compound was obtained from the crude reaction mixture in several stages: column chromatography on silica, eluting with 20% ether/80% petrol then 50% ether/50% petrol; column chromatography on silica (eluting with 25% ether/75% petrol then 40% ether/60% petrol); and finally, recrystallisa- tion from DCM gave yellow powder (0.030 g, 10%) m.p. 187-189°C (decomp.) (Found: C, 73.24;
H, 6.53; N, 7.93. C21H22N2O2.0.15CH2Cl2 requires C, 73.18; H,
6.47; N, 8.07%); δH([2H6]-DMSO) 10.95 (1H, s, 1-NH), 10.57 (1H, s, 5-NH), 8.05 (1H, d, J 8, 9-H), 7.88 (1H, s, 10-H), 7.29-7.43
(2H, m, 6-H, 7-H), 7.05 (1H, ddd, J 8, 6, 1, 8-H), 2.89 and 2.87
(2 × 3H, 2 × s, 3-CH3 and 4-CH3) and 1.59 (9H, s, C(CH3)3); m/z
(%) 355(62, M+1+), 278(90), 233(38), 126(32), 91(78) and
57(100); ʋmax (KBr Disc)/cm-13337, 1664 and 1240.
Example 7
Pyrrolo[3,2-b]carbazole-2-carboxylic Acid Amides a) 3.4-Dimethyl-2-(1-imidazolvcarbonyl)pyrrolo[3,2-b]carbazole
3,4-Dimethylpyrrolo[3,2-b]carbazole-2-carboxylic acid (0.280 g, 1.0 mmol) and N,N'-carbonyldiimidazole (0.164 g, 1.0 mmol) were dissolved in freshly distilled tetrahydrofuran (5 cm3) under a
nitrogen atmosphere. The resulting suspension was stirred at room temperature for two hours, and complete conversion of the acid to the imidazolide was verified by TLC. The THF was removed and the residue recrystallised from ethyl acetate to give the pr.oduct as a yellow solid (0.125 g, 38%) m.p. 252ºC
(decomp.) (Found: C, 73.17; H, 4.87; N, 16.80, C20H16N4O requires: C, 73.15; H, 4.91; N, 17.06%); δH([2H6]-DMSO) 11.53
(1H, s, 1-NH), 10.20 (1H, 8, 5-NH), 8.30 (1H, s, 2'-H), 8.12 (1H, d, J8, 9-H), 7.94 (1H, s, 10-H), 7.79 (1H, 8, 5'-H), 7.33-7.47 (2H, m, 6-H, 7-H), 7.19 (1H, s, 3'-H), 7.09 (1H, ddd,
J8, 6, 2, 8-H), 2.95 (3H, s, 3-CH3), 2.73 (3H, 8, 4-CH3); m/z (%) 261 (40); ʋmax (KBr Disc)/cm-13427, 1699 and 1242. b) Ethyl 3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxamide
3,4-Dimethylpyrrolo[3,2-b]carbazole-2-carboxylic acid (0.278 g, 1.0 mmol) was dissolved in dimethoxyethane (10 cm3) to give a yellow solution. To this were added diisopropylethylamine (0.260 g, 2.0 mmol), ethylamine hydrochloride (0.245 g, 3.0 mmol) and the tetrafluorborate salt of O-benzotriazolyl-N,N,N', N'- tetramethyluronium (TBTU) (0.482 g, 1.5 mmol) to give a white suspension in the yellow solution. The reaction mixture was stirred at room temperature for 24 h by which time TLC showed no remaining acid. The solvent was removed in vacuo to give a yellow-brown solid. This was subjected to column chromatography on silica eluting firstly with DCM and then with 10% EtOAc/90% DCM to give the ethylamide product as a yellow solid (0.240 g, 79%). To remove a trace impurity, a portion was recrystallised from dichlororethane/petrol to give the compound analytically pure as a yellow powder with m.p. 235ºC (decomp.) (Found: C, 73.21; H, 6.10; N, 13.33. C19H19N3O.0.1C2H4Cl2 requires: C, 73.15; H, 6.20; N, 13.32%); δH([2H6]-DMSO) 10.72
(1H, 8, 1-NH), 10.57 (1H, 8, 5-NH), 8.09 (1H, d, J8, 9-H), 7.93 (1H, t, J5, amide N-H), 7.83 (1H, s, 10-H), 7.27-7.41 (2H, m, 6-H, 7-H), 7.06 (1H, d, J8, 8-H), 3.35 (2H, q, J 7.5, CH2CH3),
2.89 and 2.71 (2 × 3H, 2 × s, 3-CH3 and 4-CH3), 1.18 (3H, t, J 7.5, CH2CH3); m/z (%) 305 (65,M+), 260 (100); ʋmax (KBr
Disc)/cm 3314, 1603 and 1545. c) 3,4-Dimethylpyrrolo[3,2-b]carbazole-2-carboxamide
3,4-Dimethylpyrrolo[3,2-b)carbazole-2-carboxylic acid (0.556 g,
2.0mmol) was dissolved in dimethoxyethane (20ml) to give a yellow solution. To this were added diisopropylethylamine
(0.520 g, 4.0 mmol), ammonium hydrochloride (0.321 g, 6.0mmol) and the tetrafluorborate salt of O-benzotriazolyl-N,N,N',N', -tetramethyluronium (TBTU) (0.963 g, 3.0mmol) to give a white suspension in the yellow solution. The reaction mixture was stirred at room temperature for 24 hours, by which time TLC showed no remaining acid. The solvent was removed in vacuo to give a yellow-brown solid. This was subjected to column chromatography on silica (eluting with ethyl acetate/DCM, gradient 10%-30%) to give the amide product as a yellow solid
(0.350 g, 63%). To remove a trace impurity, a portion was recrystallised from ethyl acetate/petrol and then purified by preparative HPLC (column size 25 cm × 2.12 cm i.d., packed with
C8 Zorbax, gradient elution: 5% acetonitrile/95% water to 95% acetonitrile/water; detected at 340 nm) to give a yellow powder with m.p. 240°C (decomp.) δH([2H6]-DMSO) 10.82 (1H, s, 1-NH),
10.54 (1H, s, 5-NH), 8.08 (1H, d, J 7.5, 9-H), 7.84 (1H, s, 10-H), 7.29-7.43 (4H, m, 6-H, 7-H, NH2), 7.07 (1H, ddd, J 8, 5.5, 2, 8-H), 2.89 and 2.85 (2 × 3H, 2 × s, 3-CH and 4-CH3); m/z (%) 277 (62, M+), 260 (100), 232 (44); ʋmax (KBr disc) /cm-1
3317, 1628, 1595; (Found: M+, 277.1205, C17H15N3O requires 277.1215). d) Phenyl 3,4-dimethylpyrrolo[3.2-b]carbazole-2-carboxamide
3,4-Dimethylpyrrolo[3,2-b]carbazole-2-carboxylic acid (0.278 g, 1.0 mmol) was dissolved in dimethoxyethane (10ml) to give a
yellow solution. To this were added diisopropylethylamine (0.130 g, 1.0mmol), aniline (0.190 g, 2.0mmol) and the tetrafluorborate salt of O-benzotriazolyl-N,N,N',N',-tetramethyluronium (TBTU) (0.482 g, 1.5mmol) to give a white suspension in the yellow solution. The reaction mixture was stirred at room temperature for 42 hours, by which time TLC showed no remaining acid. The solvent was removed in vacuo to give a yellow solid, which was dissolved in ethyl acetate and the resulting solution washed with water. The organic layer was dried over MgSO4, concentrated, and subjected to column chromatography on silica, eluting with EtOAc/petrol (gradient elution 5%-100%) followed by recrystallisation from acetone to give the phenylamide product as a yellow powder (0.10 g, 30%) with m.p. 260 C (decomp.) (Found: C, 77.79; H, 5.26; N, 11.64. C23H19N3O requires: C, 78.16; H, 5.42; N, 11.89%); δH([2H6]-DMSO) 11.10 (1H, S, 1-NH), 10.59 (1H, 8, 5-NH), 9.96 (1H, 8, amide N-H), 8.10 (1H, d, J 7.5, 9-H), 7.89 (1H, s, 10-H), 7.79 (2H, d, J 9, 2'-H, 6'-H), 7.29-7.45 (4H, m,6-H, 7-H, 3'-H,
5'-H), 7.00-7.14 (2H, m, 8-H, 4'-H), 2.93 and 2.88 (2 × 3H, 2 × s, 3-CH3); m/z (%) 353 (46, M+), 260 (100); ʋmax (KBr disc)/cm-1 3310, 1614, 1595 and 1317.
3,4-Dimethyl-2-(hydrazinocarbonyl)pyrrolo[3,2-]carbazole
Ethyl 3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylate (500 mg) and 95% hydrazine (5 cm3) were stirred and heated at 120ºC for
6h in a Readi-Vial. The mixture was allowed to stand overnight, cooled in ice and filtered. The resulting yellow solid was washed carefully with water and dried. Yield of title compound 350mg (73%), no sharp m.p. but decomposes at 285ºC. (Found: C,
69;19; H, 5.57; N, 19.38. Calc. for C17H16N4O. 0.1H2O requires
C, 69.42; H, 5.55; N, 19.05%); δH[2H6]-DMSO) 10.80 (1H, s, exchangeable, NH), 10.55 (1H, s, exchangeable, NH), 9.20 (1H, 8, exchangeable, NH), 8.06 (1H, d, J7.5 9-H), 7.81 (1H, s, 10-H),
7.42-7.28 (2H, m, 6-H and 7-H) 7.12-7.01 ( 1H, m, 8-H, 4.5 (2H, br, s, exchangeable, NH2), and 2.4 and 2.3 (2 × s, 4-CH and
3-CH2), m/z 293 (M+1)+, FAB)].
Example 8
2-Acetyl-3.4-dimethylpyrrolo[3.2-b]carbazole
Step 1
2,4-Diacetyl-3,5-dimethylpyrrole was prepared from acetylacetone and hydroxylamine-O-sulphonic acid according to the procedure of Y.Tamura, S. Kato and M.Ikeda (Chem & Ind., 1971, 767).
Step 2
2-Acetoxymethyl-3,5-diacetyl-4-methylpyrrole
To a stirred mixture of 2,4-diacetyl-3,5-dimethylpyrrole (1.0 g), dichloromethane (35 cm3) and potassium carbonate (7.73 g) at 0-5ºC was added a solution of sulphuryl chloride (0.79 g) in dichloromethane (15 cm3). The temperature of the mixture was maintained at 0.5ºC during the addition by external cooling and then the mixture was stirred at this temperature until adjudged complete by t.I.e. (ca 2h). The mixture was then filtered and evaporated to give crude 2-chloromethyl-3,5-diacetyl-4-methylpyrrole. This material was dissolved in acetic acid (10 cm3), sodium acetate (1.83g) added, and then more acetic acid (10 cm3) added. The mixture was stirred overnight at room temperature, evaporated in vacuo. and the residue stirred with ice-cold water for 2h. A solid was collected by filtration and the filtrate extracted twice with ethyl acetate. The extracts were dried (MgSO4), evaporated and the residue combined with the solid above, to give the crude product. Chromatography on silica eluting with ethyl acetate-hexane (1:1) gave 0.075 g. of pure product as an off-white solid m.p. 112.5-114.5ºC; m/z 2388
(M++1, FAB), δH, CDCl3) 2.16 (3H, S, OCOCH3), 2.50 (3H, s, CH3), 2.53 (3H, 8, CH3), 2.62 (3H, s, CH3), 5.38 (2H, s, OCH2).
Step 3
To a solution of 2-acstoxymethyl-3,5-diacetyl-4-methylpyrrole (0.200 g) and indole (0.098 g) in dichloroethane (90 cm3) was added Montmorillonite KlO clay (0.30 g). The mixture was stirred and heated at reflux for 80 h. After cooling the clay was removed by filtration and the filtrate concentrated to ca 20 cm in vacuo. The crude product was removed by filtration and then chromatographed on silica. Elution with chloroform- methanol (60:1) yielded 0.08 g of the title compound as a yellow solid m.p. 258-260°C, m/z (EI) 276 (M+) δH([2H6]-DMSO) 2.58
(3H, S, COCH3), 2.88 (3H, 8, CH3), 2.92 (3H, s, CH3), 7.05 (1H, m, 8-H), 7.38 (2H, m, 6-H, 7-H), 7.85 (1H, s, 10-H), 8.08(1H, J, 8 Hz, 9-H), 10.6 (1H, s, NH), 11.17 (1H, s, NH) . (Found: C, 77.0; H, 5.74; N, 9.76; C18H16N2O. 0.14 EtOAc requires C, 77.2; H, 5.98; N, 9.70%.)
Example 9
Ethyl 1 , 5-dihvdroindeno[2,1-f]indole-2-carboxylate
Step 1
Ethyl 2-azido-3-fluoren-2-ylacrylate
Sodium (1.7eq) was added to absolute ethanol stirred under nitrogen at room temperature. When dissolution was complete the reaction was cooled to -10ºC and fluorene-2-carboxaldehyde (1eq) and ethyl azidoacetate (3eq) dissolved together in the minimum of tetrahydrofuran were added dropwise. The mixture was stirred at -10ºC for 20h and then quenched by the addition of
water and dichloromethane. The combined organic extracts were dried (MgSO4) and evaporated in vacuo. Flash chromatography yielded the pure product (37%) ʋmax (CHCl3)/cm-12120 and 1765.
Step 2
Ethyl 2-azido-3-fluoren-2-ylacrylate suspended in dry toluene was heated at reflux for lh, and the resulting solution was then evaporated to dryness in vacuo. The resulting mixture of ethyl 1,5-dihydroindino[2,1-f]indole-2-carboxylate and ethyl 1,10-dihydroindino[1,2-g]-indole-2-carboxylate was crystallised from ethanol, thus removing most of the [1,2 g] isomer and leaving the title compound (contaminated with approximately 30% of the [1,2-g] isomer) in the mother liquors which were evaporated to dryness. δH (CDCl3) 9.11 (1H, s, br, 1-NH), 7.82-7.76 (3H, m), 7.56-7.52 (1H, m), 7.37 (H, dd, J 1 and 7),
7.34-7.28 (1H, m), 7.25 (1H, dd, J 1 and 2), 4.45 (2H, q,
OCH2CH3), 3.97 (2H, s, CH2) and 1.46 (3H, t, OCH2CH3).
Example 10
Effect of compounds of the invention in detransformation ("flattening") assay using HT1080scc2 and HT10801c cell lines.
Cell Lines and Culture Conditions
Transformed and revertant HT1080 sub-lines, HT1080scc2 and HT10801c were obtained from the Institute of Cancer Research, Chester Beatty Laboratories, Fulham Road, London. They were maintained routinely in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% foetal calf serum (FCS) and 1% penicillin/streptomycin solution containing 10,000 units per ml. All reagents were obtained from Gibco Ltd.
Cells were incubated in tissue culture grade plastic vessels at 37ºC in 5 percent CO2 in air.
Assays for compound activity
Assays for cell proliferation/cytotoxity were carried out in tissue culture grade 96 well microtitre plates (Costar). Cells in log growth were added to the plates at a concentration of 1×10 cells per well on day 0 and serially diluted compounds were then added on day 1. Plates were then incubated at 37ºC in 5% CO in air for a further 4 days.
For quantitation of cell growth, the methylene blue biomass staining method was used, the test being read on a Multiscan plate reader at wavelength of 620nm. The morphology of the cells was checked microscopically under phase-contrast immediately before the fixation and staining with methylene blue, and by ordinary light microscopy thereafter. IC50 values for active compounds were obtained using the computer programme, GS1 and dose-response slopes were also plotted.
When compounds were tested for activity in a colony forming assay the methods used were identical to those described earlier except that serially diluted compound was added to the sloppy agar when the test was set up, and replenished at the same concentration on day 7. The test results were read on day 14.
Results
Comparative growth and morphology of HT1080scc2 and HT10801c
Growth rates in terms of cell number were similar for both lines to day 4 but thereafter HT1080scc2 cells continued to divide to reach saturation densities approximately 2 to 3 times higher than HT10801c by day 5.
Phenotypic differences between the 2 lines were clearly evident. HT10801C cells displayed a much flatter morphology than the transformed cells and only a few mitotic cells were seen in confluent areas of the cultures. HT1080scc2 cells however continued to divide with numerous mitotic cells visible after confluence.
Grown under anchorage independent conditions in soft agar, HT1080scc2 produced several large colonies whereas HT10801c cells failed to produce any colonies greater than 0.1mm in diameter.
Effects of selected compounds
A number of compounds of the invention were evaluated against the cell lines.
The compounds of the invention exhibited low toxicity with IC50 values in the range 50-100μM.
Below the results of the "flattening" assay for compounds of cell invention are shown:-
Compound SCC2 flattening (μM)
Example 3 0.04
Example 4(a) 0.04
Example 4(b) 0.04
Example 4(f) 0.8
Example 4(e) 0.8
Example 4(h) 25
Example 4(g) 25
The compounds are effective at achieving "flattening" ie de-transformation, at levels significantly below their toxicity level.
The same compounds were also tested in assays using MCF7 human breast cancer cells, A431 epidermoid carcinoma cells and A285 melanoma cells. In all cases the compounds were effective in the range 1-5μM.
Claims (10)
1) A compound of the formula (i)
and salts and physiologically functional derivatives thereof, wherein A is
, ,
X is O, S, SO, SO2, CH2, CO or NR7, wherein R7 is H, alkyl, aralkyl, aryl, alkenyl, acyl, alkynyl, sulphonyl, or substituted sulphonyl;
Y is O, S, SO, SO2, CH2, CO or NR7; R1 is COR8, COOR8, CHO,CH2OH, CH2OR9, CONH2,COHHNR10R11 ,
CONHR10, CONR10R11, COO(CH2)n NR10R11 wherein R8 is H, alkyl, aryl, substituted aryl or aralkyl, R9 is acyl or substituted acyl, R10 and R11 are independently hydrogen, alkyl or aryl and n is 1 to 4; R2 is H, COOR8, alkyl,aryl, substituted aryl or CH2CH2CO2R12 wherein R12 is alkyl or aryl; R3 and R4 are independently H,hydroxy, alkyl, haloalkyl alkoxy, halo, cyano, nitro, amino, alkyl amino, dialkyl amino, substituted alkyl, carboxyl or CO2R12; R5 is H, alkyl, substituted alkyl, aralkyl, nitro, amino, halo, cyano, CHO or COOR8; R6 is H, alkyl, aryl, aralkyl, nitro, halogen, CHO or COR13 wherein R13 is alkyl or aryl with the proviso that (i) when R2, R3, R4, R5 and R6 are all H and A is
wherein Y is NH and X is O or S, then R1 is not CO2H or CO2Et; and
( ii) when R2, R3, R4, R5 and R6 are all H and A is
wherein Y is NH, and X is O then R is not CHO;
and
(iii) Y is not O when X is 0.
2) A compound according to claim 1 in which X is O, S or NR7 , wherein R7 is H, alkyl, sulphonyl or
toluene sulphonyl;
Y is NR7; R1 is COR8, COOR8, CH2OR9, CONH2, CONHNR10R11, CONHR10,
CONR10R11, COO(CH2)n NR10R11 wherein R8 is H, alkyl, aryl, substituted aryl or aralkyl, R9 is acyl or substituted acyl, R 10 and R11 are independently hydrogen, alkyl or aryl and n is 1 to 4 caroon atoms;
R 2 is COOR8, alkyl or CH2CH2CO2R12 wherein R12 is alkyl or aryl; R3 and R4 are independently H, hydroxy, alkyl, alkoxy, halo, cyano, suostituted alkyl or carboxyl; R5 is H or alkyl; R6 is H, alkyl or aryl; together with salts and physiologically functional derivatives thereof .
A compound according to claims 1 or 2 in which
X is S or NH;
Y is NH;
A is
R 1 is COOR8 wherein R8 is alkyl, or aralkyl;
R2 is H or alkyl;
R3 is- H, alkoxy, or halo;
R4 is H, alkoxy or halo;
R5 ia alkyl; R6 is hydrogen; and salts and physiologically functional derivatives thereof. 4. A compound selected from
3-pyridyl 3 ,4-dimethylpyrrolo(3,2-b]earbazole-2-earboxylate;
[(3-dimethylamino)phenyl]3,4-dimethylpyrrolo(3,2-b]carbazole-2- carboxylate;
benzyl 1,3,4-trimethylpyrrolo(3,2-bJcarbazole-2-carboxylate;
phenyl 3 , 4-dimsthylpyrrolo( 3,2-bJcarbazole-2-carboxylate;
3,4-dimethyl-2-(1-imidazolylcarbonyl)pyrrolo(3,2-b]carbazole; ethyl 3, 4-dimethylpyrrolo [3,2,-b]carbazole-2-carboxylate;
ethyl 3,
4-dimethylbenzothieno{4,
5-f.]indole-2-carboxylate;
benzyl 3,4-dimethylpyrrolo[3,2-b]carbazole-2-carboxylate;
benzyl 8-fluoro-3,4-dimethylpyrrolo{3,2-b]carbazole-2-carboxylate;
ethyl 8-fluoro-3,4-disethylpyrrolo[3,2-b]carbazole-2-carboxylats; benzyl 3,4,6-trimethylpyrrolo(3,2-b]carbazole-2-carboxylate;
ethyl 3,4,6-trimethylpyrrolo[3,2-b]carbazole-2-carboxylate;
8-fluoro-3,4-disethylpyrrolo(3,2-b]carbazole-2-carboxylic acid;
3,4-dimethylpyrrolo(3,2-b]carbazole-2-carboxylic acid;
ethyl 8-msthoxy-3,4-disethylpyrrolo(3,2-b]carbazole-2-carboxylate;
3,4,6-trimethylpyrrolo(3,2-b]carbazole-2-carboxylic acid; and benzyl 8-methoxy-3, 4-dissthylpyrrolo[3,2-b]carbazole-2-carboxylate; and salts and physiologically functional derivatives thereof
A pharmaceutical formulation which comprises a cααipound of formula (I) according to claim 1 together with a pharmaceutically acceptable carrier thereof.
6. A compound of formula (I) according to claims 1 to 4 for use in medicine.
7. Use of a compound of formula (I) or a pharmaceutically acceptable salt or physiologically functional derivative thereof for the manufacture of a medicament for the treatment of tumours.
A method of treatment of tumours in animals, which comprises the administration of an effective amount of a compound of formula (I) or a salt or physiologically functional derivative thereof.
A process for preparing compounds of general formula (I) as described in claim 1 which process comprises :
(a) catalysed ring closure of compounds of formula (IV) in the presence of a strong acid
wherein X, Y, R1, R2, R3, R4 and R5 are as defined herein; or
(b) conversion of one compound of formula (I) into another compound of formula (I).
10. Novel intermediates of formula (II), (III), (IV) or (V)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB929215361A GB9215361D0 (en) | 1992-07-20 | 1992-07-20 | Heterocyclic compounds |
GB9215361 | 1992-07-20 | ||
PCT/GB1993/001512 WO1994002483A1 (en) | 1992-07-20 | 1993-07-19 | Tetracyclic compounds process and intermediates for their preparation and their use as antitumour agents |
Publications (1)
Publication Number | Publication Date |
---|---|
AU4579593A true AU4579593A (en) | 1994-02-14 |
Family
ID=10718981
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU45795/93A Abandoned AU4579593A (en) | 1992-07-20 | 1993-07-19 | Tetracyclic compounds process and intermediates for their preparation and their use as antitumour agents |
Country Status (23)
Country | Link |
---|---|
EP (1) | EP0651750A1 (en) |
JP (1) | JPH08502037A (en) |
KR (1) | KR950702563A (en) |
CN (1) | CN1088209A (en) |
AU (1) | AU4579593A (en) |
BG (1) | BG99359A (en) |
CA (1) | CA2140662A1 (en) |
CZ (1) | CZ14995A3 (en) |
FI (1) | FI950229A (en) |
GB (1) | GB9215361D0 (en) |
HR (1) | HRP931066A2 (en) |
HU (1) | HUT76787A (en) |
IL (1) | IL106385A0 (en) |
MX (1) | MX9304355A (en) |
NO (1) | NO950202L (en) |
NZ (1) | NZ254207A (en) |
PL (1) | PL307169A1 (en) |
RU (1) | RU95104939A (en) |
SI (1) | SI9300390A (en) |
SK (1) | SK7595A3 (en) |
WO (1) | WO1994002483A1 (en) |
YU (1) | YU50293A (en) |
ZA (1) | ZA935213B (en) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9401921D0 (en) * | 1994-02-01 | 1994-03-30 | Wellcome Found | Heterocyclic compounds |
GB9413758D0 (en) * | 1994-07-07 | 1994-08-24 | Wellcome Found | Heterocyclic compounds |
GB9804343D0 (en) * | 1998-02-27 | 1998-04-22 | Univ Cardiff | Chemical compounds |
US7419992B2 (en) | 2001-02-14 | 2008-09-02 | Wisconsin Alumni Research Foundation | Use of aryl hydrocarbon receptor ligand as a therapeutic intervention in angiogenesis-implicated disorders |
ES2327615T3 (en) * | 2001-02-14 | 2009-11-02 | Wisconsin Alumni Research Foundation | PREPARATIONS AND USE OF A BINDING RECEIVER OF AH, METHYL ESTER OF ACID 2- (1'H-INDOL-3'-CARBONIL) -TIAZOL-4-CARBOXILICO. |
GB0302094D0 (en) | 2003-01-29 | 2003-02-26 | Pharmagene Lab Ltd | EP4 receptor antagonists |
GB0324269D0 (en) | 2003-10-16 | 2003-11-19 | Pharmagene Lab Ltd | EP4 receptor antagonists |
US20100130579A1 (en) * | 2006-10-23 | 2010-05-27 | Georgetown University | Cancer therapy |
WO2010019271A1 (en) | 2008-08-15 | 2010-02-18 | Georgetown University | Fluorescent regulators of rassf1a expression and human cancer cell proliferation |
JP2012051804A (en) * | 2008-12-26 | 2012-03-15 | Kyoto Univ | Eg5 INHIBITOR |
KR20160066490A (en) | 2014-12-02 | 2016-06-10 | 주식회사 씨앤드씨신약연구소 | Heterocyclic derivatives and use thereof |
US11053255B2 (en) | 2015-06-22 | 2021-07-06 | Georgetown University | Synthesis of mahanine and related compounds |
CN109776550B (en) * | 2019-01-17 | 2021-08-10 | 广东轻工职业技术学院 | Alkaloid compound derived from marine fungi and application thereof in preparation of food preservative |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0300688A1 (en) * | 1987-07-21 | 1989-01-25 | FISONS plc | Pyrrole derivatives, process for their preparation and pharmaceutical compositions containing them |
GB2239013B (en) * | 1989-11-01 | 1993-06-02 | Toyama Chemical Co Ltd | Novel isoindole derivatives and salts thereof,processes for producing the same and antitumor agent comprising the same |
WO1991014688A1 (en) * | 1990-03-20 | 1991-10-03 | The Wellcome Foundation Limited | Hetero polycyclic biocidal compounds and their use in medecine |
-
1992
- 1992-07-20 GB GB929215361A patent/GB9215361D0/en active Pending
-
1993
- 1993-07-19 MX MX9304355A patent/MX9304355A/en not_active IP Right Cessation
- 1993-07-19 CZ CZ95149A patent/CZ14995A3/en unknown
- 1993-07-19 CN CN93116598A patent/CN1088209A/en active Pending
- 1993-07-19 EP EP93916105A patent/EP0651750A1/en not_active Withdrawn
- 1993-07-19 ZA ZA935213A patent/ZA935213B/en unknown
- 1993-07-19 PL PL93307169A patent/PL307169A1/en unknown
- 1993-07-19 WO PCT/GB1993/001512 patent/WO1994002483A1/en not_active Application Discontinuation
- 1993-07-19 IL IL106385A patent/IL106385A0/en unknown
- 1993-07-19 JP JP6504272A patent/JPH08502037A/en active Pending
- 1993-07-19 KR KR1019950700226A patent/KR950702563A/en not_active Application Discontinuation
- 1993-07-19 HU HU9500171A patent/HUT76787A/en unknown
- 1993-07-19 CA CA002140662A patent/CA2140662A1/en not_active Abandoned
- 1993-07-19 NZ NZ254207A patent/NZ254207A/en unknown
- 1993-07-19 HR HR931066A patent/HRP931066A2/en not_active Application Discontinuation
- 1993-07-19 AU AU45795/93A patent/AU4579593A/en not_active Abandoned
- 1993-07-19 RU RU95104939/04A patent/RU95104939A/en unknown
- 1993-07-19 SK SK75-95A patent/SK7595A3/en unknown
- 1993-07-19 SI SI9300390A patent/SI9300390A/en unknown
- 1993-07-20 YU YU50293A patent/YU50293A/en unknown
-
1995
- 1995-01-18 BG BG99359A patent/BG99359A/en unknown
- 1995-01-19 FI FI950229A patent/FI950229A/en unknown
- 1995-01-19 NO NO950202A patent/NO950202L/en unknown
Also Published As
Publication number | Publication date |
---|---|
NZ254207A (en) | 1997-03-24 |
KR950702563A (en) | 1995-07-29 |
ZA935213B (en) | 1995-01-19 |
GB9215361D0 (en) | 1992-09-02 |
IL106385A0 (en) | 1993-11-15 |
CN1088209A (en) | 1994-06-22 |
RU95104939A (en) | 1996-10-27 |
FI950229A0 (en) | 1995-01-19 |
YU50293A (en) | 1997-01-08 |
SK7595A3 (en) | 1995-07-11 |
EP0651750A1 (en) | 1995-05-10 |
PL307169A1 (en) | 1995-05-15 |
BG99359A (en) | 1995-11-30 |
FI950229A (en) | 1995-03-10 |
WO1994002483A1 (en) | 1994-02-03 |
SI9300390A (en) | 1994-03-31 |
JPH08502037A (en) | 1996-03-05 |
MX9304355A (en) | 1994-04-29 |
HUT76787A (en) | 1997-11-28 |
NO950202D0 (en) | 1995-01-19 |
HU9500171D0 (en) | 1995-03-28 |
CA2140662A1 (en) | 1994-02-03 |
HRP931066A2 (en) | 1995-12-31 |
NO950202L (en) | 1995-01-19 |
CZ14995A3 (en) | 1995-10-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5156150B2 (en) | Novel polycyclic compounds and their use | |
AU776475C (en) | Isomeric fused pyrrolocarbazoles and isoindolones | |
JP4555476B2 (en) | Condensed azepinone-type cyclin-dependent kinase inhibitor | |
AU4579593A (en) | Tetracyclic compounds process and intermediates for their preparation and their use as antitumour agents | |
CA2140440A1 (en) | 2-thioindoles (selenoindoles) and related disulfides (selenides) which inhibit protein tyrosine kinases and which have antitumor properties | |
KR20020015077A (en) | Condensed indoline derivatives and their use as 5HT, in particular 5HT2C, receptor ligands | |
FI79319B (en) | FOERFARANDE FOER FRAMSTAELLNING AV NYA TERAPEUTISKT ANVAENDBARA TRICYKLISKA OXINDOLKARBOXAMIDDERIVAT. | |
US5679694A (en) | Tetracyclic compounds, intermediates for their preparation and their use as antitumor agents | |
EP0591058B1 (en) | Ellipticine derivatives with antitumor activity | |
KR19990007836A (en) | Fused Imidazo [1, 2-a] pyridine | |
US6201129B1 (en) | Tricyclic derivatives and their use as anti-cancer agents | |
WO2001014384A1 (en) | Tricyclic dihydrobenzofuran derivatives, process for the preparation thereof and agents | |
JP3497168B2 (en) | 2-aminoalkyl-5-aminoalkylamino-substituted isoquinoindazol-6- (2H) -ones having antitumor activity | |
US6013807A (en) | Antitumor tetracyclic compounds | |
NO310149B1 (en) | Condensed polycyclic heterocycle derivatives, preparation and use of such compounds, as well as preparations and agents comprising such compounds | |
CN113195492B (en) | Novel compound with three-fused ring structure, preparation method and application thereof | |
WO1995021171A1 (en) | Tricyclic derivatives and their use as anti-cancer agents | |
IE910333A1 (en) | Hexahydropyrrolo[2,3-b]indole carbamates, -ureas, -amides¹and related compounds, a process for their preparation and¹their use as medicaments | |
JP2003506376A (en) | 1,2-dihydro-1-oxo-pyrazino [1,2-a] indole derivatives | |
KR20050056251A (en) | Novel substituted [1,4]benzodioxino[2,3-e]isoindole derivatives, method for preparing same and pharmaceutical compositions containing same | |
CA2211979A1 (en) | Tricyclic derivatives and their use as anti-cancer agents | |
JANIN et al. | Synthesis and biological properties of new benz [h] isoquinoline derivatives | |
LU84775A1 (en) | NOVEL CONDENSED CYCLOALIPHATIC DERIVATIVES OF PYRIDO (1,2-A) SUBSTITUTED PYRIMIDINES | |
JP2001514653A (en) | Heterocyclic substances | |
AU5355290A (en) | Hetero polycyclic biocidal compounds and their use in medicine |